scholarly journals Assessment of the Percentages of T-lymphocyte Subsets in the Peripheral Blood of Mediterranean Spotted Fever Patients

2020 ◽  
Vol 18 (2) ◽  
pp. 111-119
Author(s):  
Iv. Baltadzhiev ◽  
P. Pavlov
Keyword(s):  
T Cells ◽  
Disease Severity ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
Spotted Fever ◽  
Healing Process ◽  
Cell Subset ◽  
Rickettsia Conorii ◽  
Mediterranean Spotted Fever ◽  
T Lymphocyte Subsets

Purpose: Mediterranean spotted fever (MSF) is a rickettsial disease. The aim was to evaluate the host immunе response to Rickettsia conorii. Material and methods: 62 patients were assigned into three groups: with mild, moderate or severe clinical forms of MSF. Controls were 32 healthy individuals. The diagnosis of MSF was confirmed by the indirect immunofluorescence assay. Immunophenotyping was performed using Epics XL-MCL Coulter. Results: The percentage of immune competent (CD3+) cells decreased, whereas that of helper/inducer (CD3+CD4+) and suppressor/cytotoxic (CD3+CD8+) did not change compared to controls. All three T-cell subset percentages did not parallel the disease severity. Naïve T-cells (CD4+CD45RA+) showed reduced levels, whereas activated memory (CD4+CD45RO+) T-cells did not change significantly. The percentage of activated (CD3+HLA-DR+) T-cells increased regardless of the disease severity, till the rise of stimulatory molecules (CD38+total) matched the disease severity forms. The percentage of costimulatory CD28-molecules corresponded to the disease severity as their levels increased significantly in mild forms and showed an evident downward trend towards the severe ones. Conclusion: Reduced T-lymphocyte subsets are likely related to trans-migration into perivascular inflammatory foci. The increased percentage of T-lymphocytes armed with stimulatory molecules probably reflects the mobilization of cell-mediated immune response in the healing process.

scholarly journals Clinical Investigations. T-Lymphocyte Subset Absolute Counts in the Peripheral Blood of Mediterranean Spotted Fever Patients: Relations to Disease Severity / Абсолютное Количество Субпопуляций Т-Лимфоцитов В Перифери- Ческой Крови Пациентов Со Средиземноморской Пятнистой Лихо- Радкой: Установление Связи С Тяжестью Заболевания

Folia Medica ◽  
2015 ◽  
Vol 57 (2) ◽  
pp. 93-103 ◽  
Author(s):  
Ivan G. Baltadzhiev ◽  
Pavel I. Pavlov
Keyword(s):  
T Cell ◽  
Disease Severity ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
Spotted Fever ◽  
Lymphocyte Subset ◽  
T Cell Subsets ◽  
Mediterranean Spotted Fever ◽  
T Lymphocyte Subsets ◽  
The Absolute

AbstractINTRODUCTION: Mediterranean spotted fever (MSF) in Bulgaria is caused by Rickettsia conorii conorii. Aim: This study aims at investigating the absolute counts of T-lymphocyte subsets in the peripheral blood of patients with MSF in order to establish relationships with disease severity. MATERIALS AND METHODS: The absolute counts of T-lymphocyte subsets were tested in the blood of 62 patients in the acute stage of MSF. They were assigned into three age and sex matched groups, based on the severity of disease - with mild, moderate or severe forms. Controls were 32 age and sex matched healthy individuals. The diagnosis was confirmed by an immunofluorescence assay. Immunophenotyping was performed using Epics XL-MCL Coulter, USA flow-cytometer. RESULTS: The absolute counts of immune competent (CD3+) cells, as well as the counts of helper/inducer (CD3+CD4+) and suppressor/ cytotoxic (CD3+CD8+) T-cell subsets decreased in parallel with disease severity. Naïve (CD4+CD45RA+) and activated memory (CD4+CD45RO+) T-cell subsets were reduced, particularly in severe MSF. Taken as a whole, the counts of activated (CD3+HLA-DR+) and that of presenting accessory (CD28+) or stimulatory (CD38+) molecules Т-cell subsets was increased, but in the first two subsets the trend from mild to severe forms of the disease was descending. CONCLUSION: Reduced T-lymphocyte subset counts are likely related to trans-migration into perivascular inflammatory foci. The increased number of T-lymphocytes bearing activation molecules reflects a mobilization of the cell-mediated immune response. An important issue of this study is the possible prognostic value of T-cell subsets counting, predicting the evolution of a clinical condition to clinical forms, according to the disease severity.

scholarly journals Prediction of the clinical outcome of COVID-19 patients using T lymphocyte subsets with 340 cases from Wuhan, China: a retrospective cohort study and a web visualization tool

2020 ◽  
Author(s):  
Qibin Liu ◽  
Xuemin Fang ◽  
Shinichi Tokuno ◽  
Ungil Chung ◽  
Xianxiang Chen ◽  
...  
Keyword(s):  
T Cells ◽  
Cohort Study ◽  
T Lymphocyte ◽  
Retrospective Cohort ◽  
Logistic Regression Model ◽  
Lymphocyte Subsets ◽  
Helper T Cells ◽  
T Lymphocyte Subsets ◽  
Web Visualization ◽  
Death Cases

AbstractBackgroundWuhan, China was the epicenter of the 2019 coronavirus outbreak. As a designated hospital, Wuhan Pulmonary Hospital has received over 700 COVID-19 patients. With the COVID-19 becoming a pandemic all over the world, we aim to share our epidemiological and clinical findings with the global community.MethodsIn this retrospective cohort study, we studied 340 confirmed COVID-19 patients from Wuhan Pulmonary Hospital, including 310 discharged cases and 30 death cases. We analyzed their demographic, epidemiological, clinical and laboratory data and implemented our findings into an interactive, free access web application.FindingsBaseline T lymphocyte Subsets differed significantly between the discharged cases and the death cases in two-sample t-tests: Total T cells (p < 2·2e-16), Helper T cells (p < 2·2e-16), Suppressor T cells (p = 1·8-14), and TH/TS (Helper/Suppressor ratio, p = 0·0066). Multivariate logistic regression model with death or discharge as the outcome resulted in the following significant predictors: age (OR 1·05, p 0·04), underlying disease status (OR 3·42, p 0·02), Helper T cells on the log scale (OR 0·22, p 0·00), and TH/TS on the log scale (OR 4·80, p 0·00). The McFadden pseudo R-squared for the logistic regression model is 0·35, suggesting the model has a fair predictive power.InterpretationWhile age and underlying diseases are known risk factors for poor prognosis, patients with a less damaged immune system at the time of hospitalization had higher chance of recovery. Close monitoring of the T lymphocyte subsets might provide valuable information of the patient’s condition change during the treatment process. Our web visualization application can be used as a supplementary tool for the evaluation.FundingThe authors report no funding.

Immune profiles of T lymphocyte subsets in adipose tissue of obese mouse induced by high-fat diet

2017 ◽  
Author(s):  
Guojun Zhang ◽  
Xia Wang ◽  
Huanhuan Feng ◽  
Aiping Sun ◽  
Shuming Sun ◽  
...  
Keyword(s):  
T Cells ◽  
T Lymphocyte ◽  
Cd8 T Cells ◽  
High Fat Diet ◽  
Lymphocyte Subsets ◽  
Density Lipoprotein ◽  
High Fat ◽  
T Lymphocyte Subsets ◽  
Adipose Tissues ◽  
Fat Pads

In this study, we analyzed high-fat diet (HFD)-induced time-course changes in proportions of T lymphocyte subsets in adipose tissue.Mice were fed with normal-fat diet (NFD) or HFD for 20 weeks. An autoanalyzer was used to assay plasma concentrations of glucose, cholesterol, triglycerides, low-density lipoprotein (LDL), and high-density lipoprotein (HDL). Stromal vascular cells were isolated from epididymal adipose tissues and labeled with antibodies for cluster of differentiation (CD) 3, CD4, CD8, interferon -g, interleukin (IL)-4, and IL-17 for fluorescence-activated cell sorter. We discovered that weight of epididymal fat pads and perirenal fat in HFD group were higher than that in NFD group. Significant changes in glucose, cholesterol, triglyceride, LDL, and HDL content were detected in sera of mice fed with HFD compared with those provided with NFD. Proportions of CD3+, CD4+, and CD8+ T cells increased significantly in adipose tissues of HFD mice compared with those of NFD mice. Proportions of T helper (Th)1 and Th17 sublineages also increased significantly in HFD group. Long-time HFD-induced obesity can increase proportions of CD3+ T, CD4+ T, and CD8+ T cells in epididymal fat pads, disrupt balance of CD4+ T lymphocyte subsets, and induce progressive Th1 and Th17 biases.

scholarly journals Effects of herpes virus carrier status on peripheral T lymphocyte subsets

Blood ◽  
1987 ◽  
Vol 70 (2) ◽  
pp. 516-523 ◽  
Author(s):  
JW Gratama ◽  
AM Naipal ◽  
MA Oosterveer ◽  
T Stijnen ◽  
HC Kluin-Nelemans ◽  
...  
Keyword(s):  
T Cells ◽  
T Lymphocyte ◽  
Herpes Virus ◽  
Lymphocyte Subsets ◽  
T Cell Subsets ◽  
Carrier Status ◽  
Nonspecific Esterase ◽  
T Lymphocyte Subsets ◽  
Herpès Virus ◽  
Virus Carrier

Abstract We studied the effects of herpes virus carrier status on peripheral blood T lymphocyte subsets in 334 healthy individuals. IgG-class antibodies against cytomegalovirus (CMV), Epstein-Barr virus (EBV), herpes simplex virus (HSV), and varicella-zoster virus (VZV) were used as markers for the carrier status of those viruses. CMV carrier status was associated with significant increases in the numbers of some T cell subsets, whereas the carrier status of EBV, HSV, and VZV had no significant effects. The 159 CMV-seropositive individuals had higher numbers of HNK1+ T cells than did the 175 CMV-seronegative individuals [mean (SD), 292 (196)/microL v 164 (89)/microL, respectively], including the CD4+HNK1+ T cells [38 (48)/microL v 9 (13)/microL, respectively] and the CD8+HNK1+ T cells [166 (146)/microL v 73 (54)/microL, respectively]. Morphological and cytochemical studies showed that the expression of HNK1 by the CD4+ and CD8+ T cells was associated with the occurrence of azurophilic cytoplasmatic granules and a loss of nonspecific esterase activity. The numbers of CD4+HNK1+ and CD8+HNK1+ T cells increased proportionally to the levels of the IgG- class CMV antibody titers. We suggest that the increased numbers of CD4+HNK1+ and CD8+HNK1+ granular T cells in CMV carriers reflect the persistent interaction between CMV and the immune system of its hosts.

scholarly journals Characteristics of lymphocyte subsets and their predicting values for the severity of COVID-19 patients

2020 ◽  
Author(s):  
Jingrong Wang ◽  
Xingqi Dong ◽  
Boting Zhang ◽  
Xinping Yang ◽  
Zhi Li ◽  
...  
Keyword(s):  
T Cells ◽  
Clinical Outcomes ◽  
T Lymphocyte ◽  
Roc Analysis ◽  
Lymphocyte Subsets ◽  
Laboratory Data ◽  
Serum Levels ◽  
Inflammatory Biomarkers ◽  
T Lymphocyte Subsets ◽  
Severity Of The Disease

AbstractSevere COVID-19 patients showed worse clinical outcomes compared to mild and moderate patients. However, effective indicators are still lacking to predict the severity of the disease. In the present study, we retrospectively analyzed the clinical and laboratory data of 16 COVID-19 patients and found that the absolute counts of three T-cells (CD3+, CD4+, and CD8+) were significantly lower in the moderate and severe patients than those in mild patients and were significantly lower in severe patients than in moderate patients on admission. With the recovery of the COVID-19, serum levels of inflammatory biomarkers (CRP, PCT, and IL6) of moderate and severe patients gradually decreased. In contrast, the counts of lymphocytes and their subsets including CD3+, CD4+, and CD8+ T cells gradually increased in severe patients, and eventually showed comparable levels with moderate patients. ROC analysis showed that the counts of CD3+, CD4+, and CD8+ T-cells with AUC > 0.9 have potential values for predicting the severity of COVID-19 patients. In conclusion, the reduction of CD3+, CD4+, and CD8+ T-cells is related to the severity of COVID-19 and dynamic detection of these T-lymphocyte subsets may help predict the outcome of the patients.

Changes in L-Selectin Expression by T Lymphocytes after Extracorporeal Photochemotherapy.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 1961-1961
Author(s):  
Manuel Ramirez ◽  
Marta Gonzalez-Vicent ◽  
Antonio Perez-Martinez ◽  
Julian Sevilla ◽  
Luis Madero ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Lymphocytes ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
Receptor Activation ◽  
P Value ◽  
T Lymphocyte Subsets ◽  
Extracorporeal Photochemotherapy ◽  
Steroid Refractory

Abstract Extracorporeal photochemotherapy (ECP) is an emerging treatment modality for steroid-refractory acute and chronic graft versus host disease (GVHD). The mechanisms by which ECP works are still not fully understood, and modulation of dendritic cell subpopulations, a shift of cytokine profile from Th1 to Th2 and an increase of T-cell regulatory (Treg) cells have been related to the ECP beneficial effect. Changes on T-lymphocyte subsets other than Treg have been reported after ECP (Biol Blood Marrow Transplant2006; 12(1 Suppl 2):22–30.). We analyzed the effect of ECP on the T lymphocyte subsets of sixteen children receiving this form of therapy. Steroid refractory GVHD was defined as failure to respond to 2 mg/Kg/day of methylprednisolone after 5 days (acute GVHD) or failure to respond to steroids or flare of disease activity upon tapering (chronic GVHD). ECP was performed by an alternative approach to that of the classical UVAR XTS system. This alternative method is based on a continuous-flow cell separator (COBE Spectra) that allow to process more mononuclear cells in a smaller volume and it is less time-consuming, both important advantages for pediatric patients. We studied the L-selectin (CD62L) and the CD45RA expression on CD4 and CD8 lymphocytes by flow cytometry. This combination allowed us to distinguish naive (TN, CD62L+CD45RA+), central memory (TCM, CD62L+CD45RA+), effector memory (TEM, CD62L+CD45RA+), and terminal differentiated T cells (TT, CD62L+CD45RA+), as described in Science2000;290:92–97. We compared the proportion of each of these four subpopulations, as well as the L-selectin positive and L-selectin negative ones, in samples collected from peripheral blood before the first (PRE) and after the last (POST) ECP procedures. Statistical analyses were done by the Wilcoxon signed-rank test. Results are shown in the following tables: CD4 SUBSETS & ECP PRE POST p value TN 6.58 ± 2.39 6.18 ± 2.96 0.2003 TCM 58.17 ± 4.49 43.85 ± 4.78 0.0268 TEM 34.64 ± 4.51 46.86 ± 4.89 0.0356 TT 0.6 ± 0.18 3.11 ± 1.46 0.1704 CD62Lpos 64.76 ± 4.4 50.03 ± 5.45 0.0268 CD62Lneg 35.23 ± 4.4 49.97 ± 5.45 0.0268 CD8 SUBSETS & ECP PRE POST p value TN 16.95 ± 3.94 12.53 ± 4.05 0.2343 TCM 28.8 ± 4.95 12.27 ± 2.86 0.0013 TEM 46.62 ± 5.79 51.4 ± 4.22 0.1477 TT 11.17 ± 3.34 23.52 ± 4.79 0.0105 CD62Lpos 45.75 ± 6.1 24.8 ± 5.34 0.0174 CD62Lneg 53.8 ± 6.07 74.92 ± 5.31 0.0174 The clinical outcome of the patients was always positive, with more than 50% achieving complete remission. The proportion of L-selectin expressing T lymphocytes significantly diminished after ECP, both in CD4 and in CD8 cells. The reason for these changes are currently unknown. L-selectin is an important T-cell homing receptor for T-cell entry into lymph nodes via high endothelial venules. Expression of CD62L is rapidly lost following T-cell receptor activation, leading to exit from the lymph node into the periphery and sites of inflammation. CD62Lneg and CD62Lpos also differ in their functional abilities, such as cytokine secretion and cytolytic potential. Our results suggest that ECP may have an impact in the trafficking patterns of T lymphocytes, redirectioning T cells from lymphoid to extralymphoid organs. In addition, ECP was associated to a redistribution of the pool of non-naive T lymphocytes.

Association Of Chemokine Receptor CCR5, CCR6 and CCR7 Expressions On T Lymphocyte Subsets In Recipients After Allo-HSCT With Acute GvHD

Blood ◽  
2013 ◽  
Vol 122 (21) ◽  
pp. 4596-4596 ◽  
Author(s):  
Meng Wang ◽  
Han-Yun Ren ◽  
Yu-Jun Dong ◽  
Ze-Yin Liang ◽  
Zhi-Xiang Qiu ◽  
...  
Keyword(s):  
T Cells ◽  
Stem Cell ◽  
T Lymphocyte ◽  
Chemokine Receptors ◽  
Acute Gvhd ◽  
Lymphocyte Subsets ◽  
Control Group ◽  
T Lymphocyte Subsets ◽  
Hematopoietic Stem ◽  
Grade Ii

Introduction To study the correlation of chemokine receptors expression on T lymphocyte subsets in recipients after allogeneic hematopoietic stem cell transplantation (allo-HSCT) with acute graft-versus-host disease (acute GVHD). Methods Forty-four recipients of family donor allogeneic hematopoietic stem cell transplantation were included in this study. According to the severity of acute GVHD, the recipients were divided into Grade II-IV acute GVHD group (n=16) and Grade 0-I acute GVHD group (n=28). Additionally,fifty healthy donors were also included in this study as the control group (n=50). The expression of chemokine receptors (CCR2,CCR5,CCR6,CCR7,CCR9 and CXCR3) on CD4+ and CD8+T cells in the peripheral blood after transplantation were detected using flow cytometry (FCM) respectively. The differences of the chemokine receptors expression between each group were compared. Results In both Grade 0-I acute GVHD group (on the 30th day after transplantation) and Grade II-IV acute GVHD group (at the peak of GVHD), CCR5 expression was significantly higher than that in the control group (p<0.01), and the expression of CCR7 was significantly lower than that in the control group (p<0.01). However, compared with the Grade 0-I acute GVHD group, the Grade II-IV acute GVHD group showed lower CD4+/CD8+ cell ratio (0.36 ± 0.30 vs 0.76 ± 0.61, p=0.003), higher CD4+CCR5+ ratio (65.20 ± 20.17% vs 50.82 ± 28.59%, p=0.048), decreased expression of CCR6 and CCR7 on CD4+ T cells (17.02 ± 12.43% vs 34.69 ± 18.58%, p=0.006; 20.28 ± 4.37% vs 25.45 ± 10.47%, p=0.025). Conclusions Chemokine receptors on T cell subsets are involved in the pathogenesis of acute GVHD. Expression of chemokine receptors can be used as a powerful marker for early diagnosis and differential diagnosis of acute GVHD. And targeting chemokine receptors might be the new direction of prevention and treatment of acute GVHD. Disclosures: No relevant conflicts of interest to declare.

Impact of mouse pregnancy on thymic T lymphocyte subsets

2012 ◽  
Vol 24 (8) ◽  
pp. 1123 ◽  
Author(s):  
María E. Cortina ◽  
Silvana Litwin ◽  
María E. Roux ◽  
Silvia Miranda
Keyword(s):  
T Cells ◽  
Beneficial Effect ◽  
Progenitor Cells ◽  
Pregnancy Outcome ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
T Lymphocyte Subsets ◽  
Cross Breeding ◽  
Young Females ◽  
Hormonal Effects

It has been reported that fetal lymphoid progenitor cells are acquired during gestation and are able to develop in the maternal mouse thymus into functional T cells. Moreover, previous pregnancies increase the number of fetal cells in the mother. In the present study, we investigated whether mouse pregnancy induces changes in T lymphocyte subsets in the maternal thymus. We determined the T lymphocyte subsets in two allogeneic cross-breedings, namely CBA/J × BALB/c (normal) and CBA/J × DBA/2 (abortion prone), and investigated the effects of the age and parity of the female, as well as pregnancy outcome, on thymocyte populations. In addition, hormonal effects were evaluated in a syngeneic combination (CBA/J × CBA/J). We found that during pregnancy both hormonal and allogeneic stimuli induced a reduction in the CD4+CD8+ subset with an increase in the CD4+CD8– population. Only young females of the normal combination exhibited an increase in the CD4–CD8+ population. All young mice showed an increase in CD4+CD25+FoxP3+ T cells. Interestingly, the γδT thymus pool was increased in all females of the normal allogeneic pregnancy only, suggesting the participation of this pool in the observed beneficial effect of multiparity in this cross-breeding. Our results demonstrate that allogeneic pregnancies induce important variations in maternal thymocyte subpopulations depending on the age of the female and the male component of the cross-breeding.

scholarly journals T-Lymphocyte Subsets Alteration, Infection and Renal Outcome in Advanced Chronic Kidney Disease

2021 ◽  
Vol 8 ◽  
Author(s):  
Jiachuan Xiong ◽  
Yu Qiao ◽  
Zhikai Yu ◽  
Yinghui Huang ◽  
Ke Yang ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
Renal Outcome ◽  
T Lymphocyte Subsets ◽  
Cox Regression Analysis ◽  
Cell Ratio ◽  
Low Level ◽  
Level Group

Background: T-lymphocyte subsets reflect patients' immune status and are associated with adverse outcomes in various diseases. However, the association between T-lymphocyte subsets and major infection and renal outcome in chronic kidney disease (CKD) patients has not been well-addressed.Methods: Patients diagnosed with stage 3–5 of non-dialysis CKD were recruited, and healthy subjects were selected as the controls. T-lymphocyte subsets (CD3+, CD4+, CD8+) were detected by flow cytometry, and the CD4+/CD8+ T cell ratio was then calculated. Patients were divided into the normal-level group and the low-level group according to the clinical reference value. The primary outcomes were the major infection and renal outcome.Results: A total of 410 CKD patients were enrolled; the average age was 47.25 years. Compared to the healthy controls, the level of CD3+, CD4+, CD8+ T cells, and the CD4+/CD8+ T cell ratio were significantly decreased in CKD patients (p &lt; 0.05). During the median follow-up of 2.56 (quartile interval 1.24–3.46) years, major infections occurred in 15.10% of the CKD patients. The incidence of infection was significantly higher in the low-level group of CD3+, CD4+ T cells, and CD4+/CD8+ T cell ratio compared with the normal level groups. Kaplan-Meier analysis showed that the lower level of CD3+, CD4+ T cells, and CD4+/CD8+T cell ratio is associated with a greater risk of infection. Cox regression analysis further confirmed that low CD3+, CD4+ T cells, and CD4+/CD8+ T cell ratio were independent risk factors of infection in CKD patients. Moreover, during the follow-up, renal events occurred in 37.50% of patients. Kaplan-Meier analysis indicated that low levels of CD3+, CD4+, and CD8+ T cells are significantly associated with renal outcome in CKD patients. Cox regression analysis showed that low level of CD3+ T cells (HR = 2.407, 95% CI: 1.664–3.482, p &lt; 0.001), CD4+ T cells (HR = 2.397, 95% CI: 1.633–3.518, p &lt; 0.001) and CD8+ T cells (HR = 2.416, 95% CI: 1.476–3.955, p &lt; 0.001) were independent risk factors for renal outcome after multivariable-adjusted.Conclusion: CKD patients had a defect in T-lymphocyte subpopulation. T-lymphocyte subsets were closely associated with infection and renal outcome in CKD patients. Suggesting T-lymphocyte subsets are independent predictors of infection and renal outcome in CKD patients.

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