scholarly journals Antitumor chemical constituents of Toddalia asiatica (Linn) Lam root bark and its rational alternative medicinal parts by multivariate statistical analysis

2020 ◽  
Author(s):  
Cairong Luo ◽  
Jie Liu ◽  
Yan Liang ◽  
Xiangchun Shen ◽  
Xiaoyan Zhang ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Chemical Constituents ◽  
Stem Bark ◽  
Root Bark ◽  
Multivariate Statistical ◽  
Hplc Fingerprint ◽  
Methoxycinnamic Acid ◽  
Rational Alternative ◽  
Toddalia Asiatica ◽  
Mcf 7

Abstract Toddalia asiatica (Linn) Lam (T. asiatica) as a traditional Miao medicine was investigated to find rational alternative medicinal parts for T. asiatica root bark and its antitumor chemical constituents by quantitative pharmacognostic microscopy, high performance liquid chromatography (HPLC) fingerprint and multivariate statistical analysis. A bivariate correlation analysis method based on microscopic characteristics and content of chemical constituents was established for the first time, there were some regular discoveries between powder microscopic characteristics and common chromatographic peaks of T. asiatica through quantitative pharmacognostic microscopy, cork cells, calcium oxalate square crystal, brown clump, starch granule and phloem fiber, as powder microscopic characteristics may be placed where the main chemical constitutes were enriched. Scores plot of principal component analysis (PCA) and dendrogram of hierarchical clustering analysis (HCA) showed that 18 T. asiatica samples were distinguished correctly, clustered clearly into two main groups as follows: S01∼S03 (root bark) and S07∼S09 (stem bark) in cluster 1, S04∼S06 and S10∼S18 in cluster 2. Nineteen common peaks were obtained in HPLC fingerprint of T. asiatica, loadings plot of PCA indicated seven compounds played important roles in different part of samples (P10 > P08 > P07 > P14 > P16 > P17 > P19), peaks 04, 06, 07, 08, 10 were identified as hesperidin, 4-methoxycinnamic acid, toddalolactone, isopimpinlline and pimpinellin. MTT assay was used to determine the inhibitory activity of different medicinal parts of T. asiatica on human breast cancer MCF-7 cells, all parts of T. asiatica had different inhibitory effects on MCF-7 cell lines, root and stem barks of T. asiatica showed the best inhibitory activity. The relationship between chemical constituents and the inhibitions on MCF-7 cell had been established, significant antitumor constituents of T. asiatica were identified by correlation analysis, the order of the antitumor effect of the main compounds was P07 (toddalolactone) > P16 > P06 (4-methoxycinnamic acid), P11 > P18 > P10 (pimpinellin) > P08 (isopimpinellin) > P01 > P19 > P14 > P04 (hesperidin) > P17, which were antitumor chemical constituents of T. asiatica root bark. T. asiatica stem bark was the most rational alternative medicinal part for T. asiatica root bark.

scholarly journals Studies on the Chemical Constituents of Rutaceous Plants. LXVI. The Chemical Constituents of Toddalia asiatica (L.) LAM. (T. aculeata PERS.). (1). Chemical Constituents of the Root Bark

1991 ◽  
Vol 111 (7) ◽  
pp. 365-375 ◽  
Author(s):  
Hisashi ISHII ◽  
Junichi KOBAYASHI ◽  
Munekazu ISHIKAWA ◽  
Joju HAGINIWA ◽  
Tsutomu ISHIKAWA
Keyword(s):  
Chemical Constituents ◽  
Root Bark ◽  
Toddalia Asiatica

scholarly journals Comparison of Chemical Constituents in Magnoliae Officinalis Cortex Processed by “Sweating” and “Non Sweating” based on Ultra Fast Liquid Chromatography-Triple Quadrupole-Time of Flight Mass Spectrometry and Gas Chromatography-Triple Quadrupole Mass Spectrometry Combined with Multivariate Statistical Analysis

2018 ◽  
Vol 13 (8) ◽  
pp. 1934578X1801300
Author(s):  
Hui Zhao ◽  
Ying Yan ◽  
Cheng-cheng Wang ◽  
Li-si Zou ◽  
Xun-hong Liu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Statistical Analysis ◽  
Chemical Constituents ◽  
Triple Quadrupole ◽  
Quadrupole Mass ◽  
Multivariate Statistical ◽  
Flight Mass Spectrometry ◽  
Fast Liquid Chromatography

Magnoliae Officinalis Cortex (MOC) is a commonly used traditional Chinese herbal medicine, which is always preliminarily processed by “sweating”. To explore the effects of primary processing on chemical constituents in MOC and the potential chemical markers for differentiating the samples processed by “sweating” and “non sweating”, a method is proposed based on ultra fast liquid chromatography-triple quadrupole-time of flight mass spectrometry (UFLC-Triple TOF MS/MS) and gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS) coupled with multivariate statistical analysis. The obtained data were analyzed by principal component analysis and partial least-squares discriminant analysis. The nonvolatile constituents were identified according to MS accurate mass and MS/MS spectrometry fragmentation information, combined with the software of database search and literatures comparison. The volatile constituents were identified according to the NIST05 library and literatures. All of the results demonstrated that the chemical constituents in MOC samples processed by “sweating” and “non sweating” were clearly distinguished. Seventeen nonvolatile differential constituents and five volatile differential constituents were identified and presented in different change laws. This study will provide the basic information for revealing the difference of chemical constituents in MOC processed by “sweating” and “non sweating” and comprehensive evaluation of its quality.

scholarly journals UPLC-QTOF/MS-Based Nontargeted Metabolomic Analysis of Mountain- and Garden-Cultivated Ginseng of Different Ages in Northeast China

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 33 ◽  
Author(s):  
Hailin Zhu ◽  
Hongqiang Lin ◽  
Jing Tan ◽  
Cuizhu Wang ◽  
Han Wang ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Multivariate Statistical Analysis ◽  
Chemical Constituents ◽  
Octadecenoic Acid ◽  
Chemical Components ◽  
Metabolomic Analysis ◽  
Multivariate Statistical ◽  
Similar Chemical ◽  
Different Ages ◽  
First Time

Aiming at further systematically comparing the similarities and differences of the chemical components in ginseng of different ages, especially comparing the younger or the older and mountain-cultivated ginseng (MCG), 4, 5, 6-year-old cultivated ginseng (CG) and 12, 20-year-old MCG were chosen as the analytical samples in the present study. The combination of UPLC-QTOF-MSE, UNIFI platform and multivariate statistical analysis were developed to profile CGs and MCGs. By the screening analysis based on UNIFI, 126 chemical components with various structural types were characterized or tentatively identified from all the CG and MCG samples for the first time. The results showed that all the CG and MCG samples had the similar chemical composition, but there were significant differences in the contents of markers. By the metabolomic analysis based on multivariate statistical analysis, it was shown that CG4–6 years, MCG12 years and MCG20 years samples were obviously divided into three different groups, and a total of 17 potential age-dependent markers enabling differentiation among the three groups of samples were discovered. For differentiation from other two kinds of samples, there were four robust makers such as α-linolenic acid, 9-octadecenoic acid, linoleic acid and panaxydol for CG4–6 years, five robust makers including ginsenoside Re1, -Re2, -Rs1, malonylginsenoside Rb2 and isomer of malonylginsenoside Rb1 for MCG20 years, and two robust makers, 24-hydroxyoleanolic acid and palmitoleic acid, for MCG12 years were discovered, respectively. The proposed approach could be applied to directly distinguish MCG root ages, which is an important criterion for evaluating the quality of MCG. The results will provide the data for the further study on the chemical constituents of MCG.

scholarly journals Comparison of Chemical Constituents in Pseudostellariae Radix with Different Dosage Forms Based on HPLC-Q-Exactive Orbitrap/MS Combined with Multivariate Statistical Analysis

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Yujiao Hua ◽  
Xiaoyuan Liu ◽  
Fen Xie
Keyword(s):  
Statistical Analysis ◽  
Multivariate Statistical Analysis ◽  
Chemical Constituents ◽  
Isoquinoline Alkaloid ◽  
Dosage Forms ◽  
Chemical Compositions ◽  
Multivariate Statistical ◽  
Traditional Chinese Herbal Medicine ◽  
Q Exactive ◽  
Orbitrap Ms

Background. Pseudostellariae Radix (PR) is an important traditional Chinese herbal medicine with vast clinical consumptions, which has two different dosage forms, PR decoction pieces and PR formula granules. However, these two forms are bound to have an impact on the accumulation of the effective components in PR, so the effectiveness of clinical use cannot be guaranteed. Objective. To determine the effective composition of PR. Methods. In this research, PR decoction pieces and formula granules were collected, and their composition was detected by HPLC-Q-Exactive Orbitrap/MS; multivariate statistical analysis was used to distinguish differential metabolites between PR decoction pieces and formula granules. Results. A clear cut difference in the composition of the two groups was observed. 98 differential chemical constituents could be identified in the positive mode, while 52 differential chemical compositions could be identified in the negative mode. The differential chemical compositions were mainly concentrated in flavonoids, organic acids, fatty acids, and amino acids and present different change rules, mainly involved in the isoquinoline alkaloid biosynthesis metabolic pathways. Conclusions. This study provides basic information to reveal the influence law of different dosage forms on the metabolite synthesis and quality formation mechanism of PR.

scholarly journals Insight into Seasonal Change of Phytochemicals, Antioxidant, and Anti-Aging Activities of Root Bark of Paeonia suffruticosa (Cortex Moutan) Combined with Multivariate Statistical Analysis

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6102
Author(s):  
Shicong Yang ◽  
Xiaoyan Liu ◽  
Jingyu He ◽  
Menghua Liu
Keyword(s):  
Statistical Analysis ◽  
Multivariate Statistical Analysis ◽  
Chemical Compositions ◽  
Type I ◽  
Root Bark ◽  
Aging Effects ◽  
Multivariate Statistical ◽  
Cortex Moutan ◽  
Matrix Metalloproteinase 1

Chemical compositions, antioxidants, and anti-aging activities of Cortex Moutan (CM), from different collection periods and different producing areas, were measured and compared in order to obtain excellent CM extracts. The bioactivities of CM extracts were examined by an in vitro antioxidant method and a UVB irradiated human dermal fibroblast (HDF) model. Phytochemical properties were obtained from ultra-fast liquid chromatography quadrupole time-of-flight mass spectrometry (UFLC-Q-TOF-MS) prior to the multivariate statistical analysis. As for the results, the extracts of Heze CM (HZCM) and Luoyang CM (LYCM) collected in June had better in vitro antioxidant activities, significantly increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and reduced the content of malondialdehyde (MDA), compared to other CM extracts. HZCM and LYCM extracts could upregulate the relative expression of SOD and GSH-Px mRNA. The extract of HZCM collected in June could significantly repress the production of matrix metalloproteinase 1 (MMP-1) and improve the production of procollagen type I (PCOL)-I in UVB irradiated HDF. In total, 50 compounds, including 17 monoterpenoids, 19 flavonoids, 13 phenols, and 1 amino acid were identified or tentatively identified in the CM extracts. Gallic acid, p-hydroxybenzoic acid, oxypaeoniflorin, paeoniflorin, 1,2,3,4,6-O-pentagalloyl glucose, and paeonol were predominant compounds in the CM extracts. Taken together, CM collected from April to September had better antioxidant and anti-aging effects for external usage.

scholarly journals Hemostatic chemical constituents from natural medicine Toddalia asiatica root bark by LC-ESI Q-TOF MSE

2017 ◽  
Vol 11 (1) ◽  
Author(s):  
Xiaoyan Zhang ◽  
Wenbo Sun ◽  
Zhou Yang ◽  
Yan Liang ◽  
Wei Zhou ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Root Bark ◽  
Natural Medicine ◽  
Toddalia Asiatica

scholarly journals Metabolomics Combined with Multivariate Statistical Analysis for Screening of Chemical Markers between Gentiana scabra and Gentiana rigescens

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1228
Author(s):  
Gaole Zhang ◽  
Yun Li ◽  
Wenlong Wei ◽  
Jiayuan Li ◽  
Haoju Li ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Multivariate Statistical Analysis ◽  
High Performance ◽  
Ion Trap ◽  
Chemical Constituents ◽  
Chemical Components ◽  
Chemical Markers ◽  
Multivariate Statistical ◽  
Gentiana Scabra

Gentianae Radix et Rhizome (Longdan in Chinese, GRR) in Chinese Pharmacopoeia is derived from the dried roots and rhizomes of Gentiana scabra and G. rigescens, that have long been used for heat-clearing and damp-drying in the medicinal history of China. However, the characterization of the chemical components of two species and the screening of chemical markers still remain unsolved. In current research, the identification and characterization of chemical components of two species was performed using ultra-high-performance liquid chromatography (UHPLC) coupled with linear ion trap-Orbitrap (LTQ-Orbitrap) mass spectrometry. Subsequently, the chemical markers of two species were screened based on metabolomics and multivariate statistical analysis. In total, 87 chemical constituents were characterized in G. scabra (65 chemical constituents) and G. rigescens (51 chemical constituents), with 29 common chemical constituents being discovered. Thereafter, 11 differential characteristic components which could differentiate the two species were designated with orthogonal partial least squares discriminant analysis (OPLS-DA) and random forest (RF) iterative modeling. Finally, seven characteristic components identified as (+)-syringaresinol, lutonarin, trifloroside, 4-O-β-d-glu-trifloroside, 4″-O-β-d-glucopyranosy1-6′-O-(4-O-β-d-glucaffeoyl)-linearroside, macrophylloside a and scabraside were selected as the chemical markers for the recognition of two Gentiana species. It was implied that the results could distinguish the GRR derived from different botanical sources, and also be beneficial in the rational clinical use of GRR.

scholarly journals Antimicrobial Activity and Interactions of Toddalia asiatica Isolated Coumarins with Two Known Drugs

2020 ◽  
pp. 1-16
Author(s):  
I. Onjero ◽  
B. F. Juma ◽  
J. C. Korir ◽  
R. W. Kamau
Keyword(s):  
Antimicrobial Activity ◽  
Stem Bark ◽  
Additive Effect ◽  
Spectroscopic Techniques ◽  
Root Bark ◽  
Rhizopus Stolonifer ◽  
Toddalia Asiatica ◽  
The Individual ◽  
Coumarin 1 ◽  
First Time

Five coumarins namely, 5, 7-dimethoxy-6-(3'-hydroxy-3'-methylbutan-2-oxo) coumarin coumarin (1), Toddalolactone (2), Coumurrenol (3), gleinadiene (4) and Toddaculin (5) were isolated from either the stem and/or root bark of Toddalia asiatica , with compound 1 being reported for the first time. These were obtained using chromatographic methods and identified using spectroscopic techniques, as well as comparison of their physical data with already published results. Combinations of compound 3 and fluconazole displayed additive effect in inhibiting the growth of Penicillium digitatum with reduced MIC to 125 μg/mL compared to that of fluconazole alone at 250 μg /mL. Combination of compounds 1 and 3 also showed additive effect in inhibiting Rhizopus stolonifer lowering the MIC from 500 μg/mL (for both molecules) to 250 μg /mL. Interaction in antibacterial activity between two isolated compounds 1 and 3 was also evident. These lowered the MIC in action against Staphylococcus aureus to 250 μg/mL compared to individual compounds with MIC of 500 μg/mL while showing additive effect. All the crude extracts apart from that of stem bark hexane and the individual isolated compounds showed considerable activity against all the organisms tested.

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