scholarly journals The immune system cell populations were increased in salt-induced hypertensive rats without an increase in the serum testosterone level (Short communication)

2018 ◽  
Vol 105 (2) ◽  
pp. 110-115
Author(s):  
FO Awobajo ◽  
AE Okafor ◽  
HO Adebayo
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Testosterone Level ◽  
Serum Testosterone ◽  
Male Rats ◽  
Feed Consumption ◽  
Serum Testosterone Level ◽  
Dietary Salt ◽  
Blood Samples ◽  
Cell Counts

The consumption of dietary salt has significantly increased globally, especially in the developed countries. High dietary salt intake has been linked to onset and complications in hypertension with a dimorphism tendency. There is scanty information about the influence of high salt diet on the immune cell population and androgen level in circulation. Male Sprague–Dawley rats of 8 weeks old were used for this study. They were divided into control (fed 0.1% salted feed) and salt-loaded groups (fed 8% salted feed) for 8 weeks. All experimental rats were allowed access to clean drinking water; daily feed consumption was measured in addition to weekly weight. On confirmation of hypertension using PowerLab® data acquisitions system, the rats were sacrificed and blood samples were collected into EDTA and sterile sample bottles. EDTA-blood samples were used for white blood cell and CD4 counts while the serum was used for hormonal assays. All salt-loaded rats became hypertensive, with a significant increase in total white blood cell, lymphocyte, neutrophil, monocyte, and CD4 cell counts. However, the eosinophil count was significantly decreased in salt-loaded rats. This study showed no change in the serum testosterone in salt-loaded male rats compared with control. In summary, dietary salt loading while precipitating hypertension also activated increased production of white blood cells and CD4 cells without any change in the serum testosterone level.

scholarly journals Free serum testosterone level in male rats treated with tribulus alatus extracts

2007 ◽  
Vol 33 (4) ◽  
pp. 554-559 ◽  
Author(s):  
Walid H. El-Tantawy ◽  
Abeer Temraz ◽  
Omayma D. El-Gindi
Keyword(s):  
Testosterone Level ◽  
Serum Testosterone ◽  
Male Rats ◽  
Serum Testosterone Level ◽  
Free Serum

Transient Rise of Serum Testosterone Level after Single Sildenafil Treatment of Adult Male Rats

2012 ◽  
Vol 9 (10) ◽  
pp. 2534-2543 ◽  
Author(s):  
Marija M. Janjic ◽  
Natasa J. Stojkov ◽  
Maja M. Bjelic ◽  
Aleksandar I. Mihajlovic ◽  
Silvana A. Andric ◽  
...  
Keyword(s):  
Adult Male ◽  
Testosterone Level ◽  
Serum Testosterone ◽  
Male Rats ◽  
Serum Testosterone Level ◽  
Transient Rise

A New Study of Intraosseous Blood for Laboratory Analysis

2010 ◽  
Vol 134 (9) ◽  
pp. 1253-1260 ◽  
Author(s):  
Larry J. Miller ◽  
Thomas E. Philbeck ◽  
Diana Montez ◽  
Cathy J. Spadaccini
Keyword(s):  
Blood Cell ◽  
Critically Ill ◽  
White Blood Cell ◽  
Critically Ill Patients ◽  
Blood Chemistry ◽  
Laboratory Analysis ◽  
Blood Samples ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
White Blood Cell Counts

Abstract Context.—Intraosseous (IO) blood is frequently used to establish a blood chemistry profile in critically ill patients. Questions remain regarding the reliability of IO blood for laboratory analysis and established criteria regarding the amount of marrow/blood to waste before taking an IO sample are not available. Objectives.—To evaluate IO-derived blood for routine laboratory blood tests needed in the care of critically ill patients and to determine the amount of marrow/blood to waste before drawing blood from the IO space for laboratory analysis. Design.—Blood samples were drawn from peripheral veins of 10 volunteers. Within 5 minutes, 2 IO blood samples were obtained; one following 2 mL of waste and another following 6 mL of waste. Samples were analyzed for complete blood count and chemistry profile. Values were analyzed using Pearson correlation coefficients. Levels of significance were determined using the t distribution. Mean values for the draws were calculated and compared, with the intravenous blood sample serving as a control for the IO samples. Results.—There was a significant correlation between intravenous and IO samples for red blood cell counts and hemoglobin and hematocrit levels but not for white blood cell counts and platelet counts. There was a significant correlation between intravenous and IO samples for glucose, blood urea nitrogen, creatinine, chloride, total protein, and albumin concentrations but not for sodium, potassium, CO2, and calcium levels. Conclusions.—When venous blood cannot be accessed, IO blood aspirate may serve as a reliable alternate, especially for hemoglobin and hematocrit levels and most analytes in a basic blood chemistry profile. Exceptions are CO2 levels and platelet counts, which may be lower, and white blood cell counts, which may appear elevated.

scholarly journals Leukocyte Counts Based on DNA Methylation at Individual Cytosines

2018 ◽  
Vol 64 (3) ◽  
pp. 566-575 ◽  
Author(s):  
Joana Frobel ◽  
Tanja Božić ◽  
Michael Lenz ◽  
Peter Uciechowski ◽  
Yang Han ◽  
...  
Keyword(s):  
Dna Methylation ◽  
T Cells ◽  
Blood Cell ◽  
White Blood Cell ◽  
Absolute Quantification ◽  
Blood Samples ◽  
Cell Numbers ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
White Blood Cell Counts

Abstract BACKGROUND White blood cell counts are routinely measured with automated hematology analyzers, by flow cytometry, or by manual counting. Here, we introduce an alternative approach based on DNA methylation (DNAm) at individual CG dinucleotides (CpGs). METHODS We identified candidate CpGs that were nonmethylated in specific leukocyte subsets. DNAm levels (ranging from 0% to 100%) were analyzed by pyrosequencing and implemented into deconvolution algorithms to determine the relative composition of leukocytes. For absolute quantification of cell numbers, samples were supplemented with a nonmethylated reference DNA. RESULTS Conventional blood counts correlated with DNAm at individual CpGs for granulocytes (r = −0.91), lymphocytes (r = −0.91), monocytes (r = −0.74), natural killer (NK) cells (r = −0.30), T cells (r = −0.73), CD4+ T cells (r = −0.41), CD8+ T cells (r = −0.88), and B cells (r = −0.66). Combination of these DNAm measurements into the “Epi-Blood-Count” provided similar precision as conventional methods in various independent validation sets. The method was also applicable to blood samples that were stored at 4 °C for 7 days or at −20 °C for 3 months. Furthermore, absolute cell numbers could be determined in frozen blood samples upon addition of a reference DNA, and the results correlated with measurements of automated analyzers in fresh aliquots (r = 0.84). CONCLUSIONS White blood cell counts can be reliably determined by site-specific DNAm analysis. This approach is applicable to very small blood volumes and frozen samples, and it allows for more standardized and cost-effective analysis in clinical application.

scholarly journals Evaluating Accuracy of DNA Pool Construction Based on White Blood Cell Counts

2021 ◽  
Vol 12 ◽  
Author(s):  
Amy N. Abrams ◽  
Tara G. McDaneld ◽  
John W. Keele ◽  
Carol G. Chitko-McKown ◽  
Larry A. Kuehn ◽  
...  
Keyword(s):  
Blood Cell ◽  
Dna Extraction ◽  
White Blood Cell ◽  
Univariate Analysis ◽  
White Blood Cells ◽  
Individual Animal ◽  
Blood Samples ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
White Blood Cell Counts

Pooling individual samples prior to DNA extraction can mitigate the cost of DNA extraction and genotyping; however, these methods need to accurately generate equal representation of individuals within pools. The objective of this study was to determine accuracy of pool construction of blood samples based on white blood cell counts compared to two common DNA quantification methods. Fifty individual bovine blood samples were collected, and then pooled with all individuals represented in each pool. Pools were constructed with the target of equal representation of each individual animal based on number of white blood cells, spectrophotometric readings, spectrofluorometric readings, and whole blood volume with 9 pools per method and a total of 36 pools. Pools and individual samples that comprised the pools were genotyped using a commercially available genotyping array. ASReml was used to estimate variance components for individual animal contribution to pools. The correlation between animal contributions between two pools was estimated using bivariate analysis with starting values set to the result of a univariate analysis. Adonis test on distance matrix from the animal correlation showed clustering with method, and higher correlations between methods than within (P < 1 × 10–6). White blood cell count was predictive of sample representation when compared to pooling based on DNA concentration. Therefore, constructing pools using white blood cell counts prior to DNA extraction may reduce cost associated with DNA extraction and genotyping and improve representation of individuals in a pool.

scholarly journals Effect of Quinolones on Serum Testosterone Level in Male Albino Rats

2017 ◽  
Vol 23 (1) ◽  
Author(s):  
Rubina Iqbal ◽  
Saud Iqbal ◽  
Shahzad Anjum
Keyword(s):  
Testosterone Level ◽  
Morphological Changes ◽  
Serum Testosterone ◽  
Serum Testosterone Level ◽  
Term Therapy ◽  
Control Group ◽  
Albino Rats ◽  
Distilled Water ◽  
Blood Samples ◽  
Testosterone Levels

<p><strong>Objective:</strong><strong>  </strong>To determine the toxic effects of quinolones on serum testosterone level in male albino rats.</p><p><strong>Methods:</strong><strong>  </strong>Eighty male albino rats were randomly divided into A, B, C and D groups each group having 20 albino rats. These groups were further subdivided into A1, A2, B1, B2, C1, C2, D1 and D2 having 10 albino rats each. Ciprofloxcin, ofloxacin and enoxacin dissolved in distilled water were given at 135mg/kg/ day, 72mg/kg/day and 12.5mg/kg/day to groups A, B and C respectively for 12 weeks. Only distilled water was given to group D which was control group for the same time period. Blood samples were drawn for testosterone hormone level estimation at 0, 14<sup>th</sup>, 28<sup>th</sup> and 42<sup>nd</sup> day in subgroups Al, B1, Cl and D1 and then the animals in said groups were sacrificed on 42<sup>nd</sup> day to identify testicular morphological changes. Rats in subgroup A2, B2, C2 and D2 were kept alive till 84<sup>th</sup> day after stopping drugs at 42 days to find out if there is any change in levels of testosterone after discontinuation of the treatment. Blood samples for testosterone hormone estimation were taken at 0, 14<sup>th</sup>, 28<sup>th</sup>, 42<sup>nd, </sup>56<sup>th</sup>, 70<sup>th</sup> and 84<sup>th</sup> day from subgroups A2, B2, C2 and D2. Testosterone hormone estimation assay was performed on the collected samples.</p><p><strong>Results:</strong><strong>  </strong>Testosterone assay showed significant decrease in serum testosterone levels in all experimental groups, when compared with control group. These levels did not return back to normal even after withdrawal of drugs. This study suggests a gonadotoxic potential of quinolones.</p><p><strong>Conclusion:  </strong>Quinolones reduce serum testosterone levels and should be used carefully for long term therapy.<strong></strong></p>

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