On Dactylogyridae (Monogenea) of four species of characid fishes from Brazil

Material collected from the fishes from the dams and fish farms of the Departamento Nacional de Obras contra as Secas (DNOCS) and from the tanks of the Itaipu Hydroelectric Station was examined in order to analyze the index and the infestation of Monogenea. Specimens of tambaqui (Colossoma macropomum), pacu (Piaractus mesopotamicus), pirapitinga (Piaractus brachypomus) and the hybrid tambacu (Colossoma macropomum X Piaractus mesopotamicus) were examined and five species of Monogenea were identified including: Mymarothecium viatorum Boeger, Piasecki & Sobecka, 2002, Mymarothecium boegeri Cohen & Kohn, 2005, Notozothecium euzeti Kritsky, Boeger & Jégu, 1996, Notozothecium januachensis Belmont-Jegu, Domingues & Martins, 2004 and Anacanthorus penilabiatus Boeger, Husak & Martins, 1995. Infection prevalence and intensity of infection and new data concerning Monogenea are presented. Key words: Monogenea, Parasites, Brazil, freshwater fishes.

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Perulernaea gamitanae (Copepoda: Lernaeidae) parasitizing tambaqui (Colossoma macropomum) (Characidae) and the hybrids tambacu and tambatinga, cultured in northern Brazil

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/s0102-09352011000400026 ◽

2011 ◽

Vol 63 (4) ◽

pp. 988-995 ◽

Cited By ~ 10

Author(s):

M. Tavares-Dias ◽

L.R. Neves ◽

E.F. Santos ◽

M.K.R. Dias ◽

R.G. B. Marinho ◽

...

Keyword(s):

Fish Farm ◽

The Other ◽

Infestation Rate ◽

Fish Farms ◽

Colossoma Macropomum ◽

Piaractus Mesopotamicus ◽

Attachment Sites ◽

High Prevalence ◽

Northern Brazil ◽

New Hosts

The infestation rate in Colossoma macropomum, hybrid tambacu (C. macropomum x Piaractus mesopotamicus) and hybrid tambatinga (C. macropomum x Piaractus brachypomum) with Perulernaea gamitanae Thatcher and Paredes, 1985 from two fish farms in Amapá State, Brazil was studied. Lernaeid parasites (n=2887) were collected mainly on the tongue and the mouth cavity and also on cartilage of gill arches and filaments. Inflammation and fibrous nodules were observed on the attachment sites of the parasites. The infestation rate varied according to the fish farm and host. The prevalence of P. gamitanae was of 100% in hosts from one fish farm and was lower in the other fish farm. Higher intensity of P. gamitanae occurred in hybrids tambacu and tambatinga, but despite the high prevalence its intensity was moderate. This is the first report on epidemiology of P. gamitanae in cultured fishes from Brazilian Amazonia, and the occurrence of this crustacean parasite in two new hosts, the hybrids tambacu and tambatinga.

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Integrative MicroRNAome Analysis of Skeletal Muscle of Colossoma Macropomum (Tambaqui), Piaractus Mesopotamicus (Pacu), and the Hybrid Tambacu, based on Next-generation Sequencing Data.

10.21203/rs.3.rs-51788/v1 ◽

2020 ◽

Author(s):

Bruno EA Fantinatti ◽

Erika S Perez ◽

Bruna TT Zanella ◽

Jéssica S Valente ◽

Tassiana G de Paula ◽

...

Keyword(s):

Skeletal Muscle ◽

Target Genes ◽

Good Choice ◽

Next Generation Sequencing Data ◽

Small Rna Sequencing ◽

Sequencing Data ◽

Fish Farms ◽

Colossoma Macropomum ◽

Piaractus Mesopotamicus ◽

Specific Regulation

Abstract Background: Colossoma macropomum (tambaqui) and Piaractus mesopotamicus (pacu) are good fish species for aquaculture. The tambacu, individuals originating from the induced hybridization of the female tambaqui with the male pacu, present rapid growth and robustness, characteristics which have made the tambacu a good choice for Brazilian fish farms. Here, we used small RNA sequencing to examine global miRNA expression in the genotypes pacu (PC), tambaqui (TQ), and hybrid tambacu (TC), (Juveniles, n=5 per genotype), in order to better understand the relationship between tambacu and its parental species, and also to clarify the mechanisms involved in tambacu muscle growth and maintenance based on miRNAs expression. Results: Regarding differentially expressed (DE) miRNAs between the three genotypes, we observed 8 upregulated and 7 downregulated miRNAs considering TC vs. PC; 14 miRNAs were upregulated and 10 were downregulated considering TC vs. TQ, and 15 miRNAs upregulated and 9 were downregulated considering PC vs. TQ. The majority of the miRNAs showed specific regulation for each genotype pair, and no miRNA were shared between the 3 genotype pairs, in both up- and down-regulated miRNAs. Considering only the miRNAs with validated target genes, we observed the miRNAs miR-144-3p, miR-138-5p, miR-206-3p, and miR-499-5p. GO enrichment analysis showed that the main target genes for these miRNAs were grouped in pathways related to oxygen homeostasis, blood vessel modulation, and oxidative metabolism.Conclusions: Our global miRNA analysis provided interesting DE miRNAs in the skeletal muscle of pacu, tambaqui, and the hybrid tambacu. In addition, in the hybrid tambacu, we identified some miRNAs controlling important molecular muscle markers that could be relevant for the farming maximization.

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Integrative microRNAome analysis of skeletal muscle of Colossoma macropomum (tambaqui), Piaractus mesopotamicus (pacu), and the hybrid tambacu, based on next-generation sequencing data

BMC Genomics ◽

10.1186/s12864-021-07513-5 ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Bruno E. A. Fantinatti ◽

Erika S. Perez ◽

Bruna T. T. Zanella ◽

Jéssica S. Valente ◽

Tassiana G. de Paula ◽

...

Keyword(s):

Skeletal Muscle ◽

Target Genes ◽

Good Choice ◽

Next Generation Sequencing Data ◽

Small Rna Sequencing ◽

Sequencing Data ◽

Fish Farms ◽

Colossoma Macropomum ◽

Piaractus Mesopotamicus ◽

Specific Regulation

Abstract Background Colossoma macropomum (tambaqui) and Piaractus mesopotamicus (pacu) are good fish species for aquaculture. The tambacu, individuals originating from the induced hybridization of the female tambaqui with the male pacu, present rapid growth and robustness, characteristics which have made the tambacu a good choice for Brazilian fish farms. Here, we used small RNA sequencing to examine global miRNA expression in the genotypes pacu (PC), tambaqui (TQ), and hybrid tambacu (TC), (Juveniles, n = 5 per genotype), to better understand the relationship between tambacu and its parental species, and also to clarify the mechanisms involved in tambacu muscle growth and maintenance based on miRNAs expression. Results Regarding differentially expressed (DE) miRNAs between the three genotypes, we observed 8 upregulated and 7 downregulated miRNAs considering TC vs. PC; 14 miRNAs were upregulated and 10 were downregulated considering TC vs. TQ, and 15 miRNAs upregulated and 9 were downregulated considering PC vs. TQ. The majority of the miRNAs showed specific regulation for each genotype pair, and no miRNA were shared between the 3 genotype pairs, in both up- and down-regulated miRNAs. Considering only the miRNAs with validated target genes, we observed the miRNAs miR-144-3p, miR-138-5p, miR-206-3p, and miR-499-5p. GO enrichment analysis showed that the main target genes for these miRNAs were grouped in pathways related to oxygen homeostasis, blood vessel modulation, and oxidative metabolism. Conclusions Our global miRNA analysis provided interesting DE miRNAs in the skeletal muscle of pacu, tambaqui, and the hybrid tambacu. In addition, in the hybrid tambacu, we identified some miRNAs controlling important molecular muscle markers that could be relevant for the farming maximization.

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Resistência ao estresse e crescimento de larvas de peixes neotropicais alimentadas com diferentes dietas

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2007000100009 ◽

2007 ◽

Vol 42 (1) ◽

pp. 65-72 ◽

Cited By ~ 4

Author(s):

Ronald Kennedy Luz

Keyword(s):

Colossoma Macropomum ◽

Piaractus Mesopotamicus ◽

Astronotus Ocellatus

O objetivo deste trabalho foi avaliar o efeito da alimentação na resistência ao estresse e no crescimento de larvas das espécies de peixes neotropicais: Astronotus ocellatus (Oscar), Piaractus mesopotamicus (pacu) e Pseudoplatystoma coruscans (pintado). As larvas receberam diferentes tipos de alimentos (Artemia sp., larvas de Colossoma macropomum e dieta artificial Fry Feed Kyowa). Foram realizados testes de exposição ao ar e a taxa de sobrevivência, determinada 24 horas depois. A fim de avaliar o crescimento, medidas de peso foram realizadas em larvas dos diferentes tratamentos. Larvas de A. ocellatus alimentadas com náuplios de Artemia sp. apresentaram tendência de maior peso e resistência ao estresse, quando comparadas com o uso de dieta artificial. Larvas de P. mesopotamicus apresentaram melhores valores de peso e taxas de resistência ao estresse, quando alimentadas com Artemia sp. ou alimentação mista (Artemia sp. + dieta artificial). Em P. coruscans, o uso de larvas forrageiras resultou em indivíduos mais resistentes aos testes de exposição ao ar do que os que receberam apenas Artemia sp. Valores de peso, nos dois manejos alimentares, foram semelhantes entre si. O alimento vivo desempenha importante atuação no crescimento em peso e na melhora da resistência ao estresse das espécies estudadas.

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Epidemiology of acanthocephalan infections in crabs from the Bahía Blanca Estuary, Argentina

Journal of Helminthology ◽

10.1017/s0022149x11000678 ◽

2011 ◽

Vol 86 (4) ◽

pp. 446-452 ◽

Cited By ~ 5

Author(s):

L.F. La Sala ◽

A.M. Perez ◽

S.R. Martorelli

Keyword(s):

Risk Factors ◽

Carapace Length ◽

Carapace Width ◽

Infection Prevalence ◽

Intensity Of Infection ◽

Breeding Colony ◽

Bahía Blanca Estuary ◽

Feeding Area ◽

Olrog’S Gull ◽

Blanca Estuary

AbstractThis study was conducted in two populations of crabs, Cyrtograpsus angulatus and Neohelice granulata from the Bahía Blanca Estuary, in Argentina, to identify risk factors for infection by the acanthocephalan Profilicollis chasmagnathi and to assess the association between infection and mortality of these hosts. Cyrtograpsus angulatus and N. granulata crabs were sampled seasonally over the course of a year, and spring sampling included collection of dead crabs predated by Olrog's gulls in a nearby breeding colony. Potential risk factors for infection were assessed and the number of cystacanth larvae per crab was counted. In C. angulatus, the odds of infection increased by 7% for each millimetre increase in carapace length, and were nearly 17 times greater in crabs sampled from the Olrog's gull feeding area compared with those sampled from nests in the breeding colony. For every millimetre increase in carapace length in N. granulata, the odds of infection increased by 13% in crabs from the breeding colony, and by 32% in crabs from the feeding area. Mean intensity of infection in N. granulata increased by 16.5% for each additional millimetre of carapace width. The level of parasite aggregation was lowest in the largest C. angulatus and highest in N. granulata predated by Olrog's gull. The results show that host size is the most important factor influencing infection prevalence in both crab species and intensity of infection in N. granulata, and suggest the presence of parasite-induced mortality in the populations studied.

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Prevalence and intensity of Sarcocystis spp. infection in animals slaughtered for food in Lithuania

Veterinární Medicína ◽

10.17221/151/2017-vetmed ◽

2019 ◽

Vol 64 (No. 4) ◽

pp. 149-157 ◽

Cited By ~ 3

Author(s):

V Januskevicius ◽

G Januskeviciene ◽

P Prakas ◽

D Butkauskas ◽

S Petkevicius

Keyword(s):

Light Microscopy ◽

Infection Prevalence ◽

Intensity Of Infection ◽

Sarcocystis Spp ◽

High Prevalence ◽

Muscle Types ◽

Muscle Groups

The exact prevalence of Sarcocystis spp. infection in animals slaughtered for food is unknown in Lithuania. Therefore, the present study was initiated to evaluate Sarcocystis spp. infection in the carcasses of cattle (n = 206), sheep (n = 61), pigs (n = 73) and horses (n = 72) raised in Lithuania for food. The prevalence and intensity of Sarcocystis spp. infection were assessed under light microscopy by analysing 1 g of stained and squashed muscle samples. All the investigated muscle types (oesophagus, diaphragm, heart, neck, jaw, back, leg and tongue) were found to have been infected with microcysts rather than with macrocysts. A high prevalence of infection was established in cattle (44.9–98.1%) and sheep (100%), whereas the prevalence of this infection in pigs (30.1–50.0%) and horses (34.7–63.9%) was considered to be moderate. Significant differences in the infection prevalence were detected in the majority of muscle groups of cattle and in some muscle groups of pigs and horses. Similarly, significant differences in the median (Md) intensity of infection were observed in the majority of the muscle groups of cattle (Md = 4–29) and sheep (Md = 21–73) and only in some muscle groups of pigs (Md = 4.5–16) and horses (Md = 1–3). Cases of intense infection (> 40 cysts in a sample) were relatively often detected in sheep (44.9%) and cattle (19.1%), and rarely in pigs (3.7%). Hence, based on the varying rates of infection in the examined samples, the infection was identified as being intense in sheep and cattle, moderate in pigs and low in horses.

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Cross-amplification of heterologous microsatellite markers in Piracanjuba

Ciência Rural ◽

10.1590/0103-8478cr20170374 ◽

2017 ◽

Vol 47 (12) ◽

Cited By ~ 3

Author(s):

Pedro Luiz de Castro ◽

Ricardo Pereira Ribeiro ◽

Silvio Carlos Alves dos Santos ◽

Elenice Souza dos Reis Goes ◽

Felipe Pinheiro de Souza ◽

...

Keyword(s):

Genetic Variability ◽

Microsatellite Markers ◽

Oreochromis Niloticus ◽

Genetic Parameters ◽

Economic Value ◽

Current Analysis ◽

Genetic Studies ◽

Colossoma Macropomum ◽

Piaractus Mesopotamicus ◽

Cross Amplification

ABSTRACT: Brycon orbignyanus, popularly known in Brazil as piracanjuba, is a fish with great economic value but whose natural population drastically decreased in number during the last years. In this context, genetic variability studies of natural stocks and in restocking programs are fundamental for the adoption of conservation measures. Current analysis verifies the cross-amplification of heterologous primers in B. orbignyanus. Fifty-two primers of the species Brycon opalinus, Brycon hilarii, Brycon insignis, Prochilodus sp., Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus were tested. Primers with the best reproducibility were applied to a sample of 20 individuals and the genetic parameters were calculated. Nine primers provided good results for cross-amplification with B. orbignyanus, involving (BoM5 and BoM13) of Brycon opalinus, (Bh5, Bh6, Bh8, Bh13 and Bh16) of Brycon hilarii, (Bc48-10) of Brycon insignis and (Par80) of Prochilodus argenteus. Primers of Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus failed to provide amplification or provided non-specificity. Results demonstrated the possibility of using primers of different species and genera of B. orbignyanus, facilitating genetic studies on the species.

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Conhecimento ecológico tradicional da ictiofauna pelos quilombolas no Alto Guaporé, Mato Grosso, Amazônia meridional, Brasil

Boletim do Museu Paraense Emílio Goeldi Ciências Humanas ◽

10.1590/1981.81222018000200004 ◽

2018 ◽

Vol 13 (2) ◽

pp. 315-329 ◽

Cited By ~ 2

Author(s):

Joari Costa de Arruda ◽

Carolina Joana da Silva ◽

Nilo Leal Sander ◽

María Teresa Pulido

Keyword(s):

Mato Grosso ◽

Colossoma Macropomum ◽

Piaractus Mesopotamicus ◽

Hoplias Malabaricus ◽

Astronotus Ocellatus ◽

Pygocentrus Nattereri

Resumo O objetivo desta pesquisa foi estudar o conhecimento ecológico tradicional (CET) relacionado à pesca feita por grupo étnico autoidentificado como quilombola, por meio da análise de fatores que influenciam o consenso cultural e as técnicas da pesca artesanal. Participaram 24 homens e sete mulheres dos quilombos Retiro, Boqueirão, Casalvasco e Manga. Os métodos de amostragem utilizados foram: bola de neve, lista livre e observação participante. Os resultados incluem 47 etnoespécies (46 peixes e um réptil). O consenso cultural é composto por: Hoplias malabaricus, Cichla sp., Pseudoplatystoma fasciatum, Astronotus ocellatus, Satanoperca pappaterra, Brycon cephalus, Piaractus mesopotamicus, P. corruscans, Pimelodus sp., Colossoma macropomum, Pygocentrus nattereri, Mylossoma aureum, Astyanax sp. e Leporinus freiderici. Embora tenha sido maior a riqueza do conhecimento entre pessoas que residiram temporariamente em outros estados brasileiros, este aspecto não esteve relacionado à idade dos entrevistados. A manutenção e a transmissão de CET é propiciada por contato diário com peixes e com o rio desde criança, bem como por observação das práticas de pesca em grupo. O CET é parte da identidade e representa uma herança cultural que deve ser valorizada. É necessária a participação desses atores sociais nos planos de decisão e de gerenciamento de áreas protegidas.

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Parasitic infections in tambaqui from eight fish farms in Northern Brazil

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-4162-7592 ◽

2015 ◽

Vol 67 (4) ◽

pp. 1070-1076 ◽

Cited By ~ 11

Author(s):

M.K.R. Dias ◽

L.R. Neves ◽

R.G.B. Marinho ◽

M. Tavares-Dias

Keyword(s):

Water Quality ◽

Parasite Species ◽

Parasitic Infection ◽

Parasitic Infections ◽

Ichthyophthirius Multifiliis ◽

Fish Farms ◽

Colossoma Macropomum ◽

Northern Brazil

The aim of this study was to evaluate the parasites and parasite-host relationship in Colossoma macropomum from eight fish farms in Amapá State, Northern Brazil. A total of 12,242,559 parasites were recovered from hosts in the eight fish farms, and 87.4% of fish were infected by one or more parasite species, such as Ichthyophthirius multifiliis, Piscinoodinium pillulare, Trichodinasp. and Tetrahymena sp. (Protozoa), Anacanthorus spathulatus, Linguadactyloides brinkmanni, Mymarothecium boegeri and Notozothecium janauachensis (Monogenoidea), Procamallanus(Spirocamallanus) inopinatus (Nematoda) and Perulernaea gamitanae and Braga patagonica (Crustacea). Ichthyophthirius multifiliis presented dominance, but the greatest levels of parasitism were caused by I. multifiliis, P. pilulare, monogenoideans species and P. gamitanae, and all these parasites had an aggregate dispersion. The eight fish farms have differences in the parasitic infection levels, management and water quality, which are further discussed here.

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