scholarly journals The effect of Myrmecodia pendans extract toward gastric histology in white rats treated with toxic dose of gentamicin: a preliminary report

2020 ◽  
Vol 9 (3) ◽  
pp. 924
Author(s):  
I Made Bagus Januar Bahari Putra ◽  
Ketut Budiasa ◽  
I Made Merdana ◽  
Anak Agung Ayu Mirah Adi
Keyword(s):  
Preliminary Report ◽  
Toxic Dose ◽  
White Rats

scholarly journals HISTOPATHOLOGICAL STRUCTURE OF WHITE RATS LIVER AFTER GIVING ANT NEST EXTRACT DUE TO INDUCED PARACETAMOL TOXIC DOSE

2019 ◽  
pp. 14
Author(s):  
I Made Merdana ◽  
I Made Kardena ◽  
Ketut Budiasa ◽  
I Made Dodi Gunawan
Keyword(s):  
Rat Liver ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Toxic Dose ◽  
White Rats ◽  
Significant Difference ◽  
Ant Nest ◽  
Histopathologic Changes

This study aim was to determine the influence ant nest plant extract (Myrmecodia pendans) on histopathological changeof white rat liver (Rattus novergicus) due to induced with paracetamol toxic dose. This study used 24 male white rats, divided into four groups, negative control group (P0) given placebo, positive control group (P1) given paracetamol dose 250 mg / kg bw for 10 days, P2 given ant nest extract 250 mg / kg bw and paracetamol dose 250 mg / kg bw for 10 days, P3 given ants nest extract 250 mg / kg bw for seven days, then continued by giving paracetamol and ants nest extract with dose 250 mg / kg bw for ten days. After the treatment done, all the rats were dinecropsed. Liver organs were taken and processed for making histopathology preparations. Parameters examined included hemorrhage, congestion, degeneration and necrosis. The data obtained were analyzed statistically by using Kruskal Wallis test followed by Mann Whitney test. Mann Whitney test results for all categories of histopathologic changes in  hemorrhagic, congestion, degeneration, and necrosis between negative control group (P0) and positive control group (P1) were significantly different (P <0.05), between negative control (P0) with P2 and P3 there was no significant difference (P> 0,05). Afterward, between the positive control (P1) and P2 with P3 there was a significant difference (P <0.05). I can be concludedthat the administration of paracetamol dose 250 mg/kg bw for 10 days affects the histopathologic changes of white rat liver. The administration of ant nest plant extracts can reduce the side effects of toxic doses of paracetamol.

scholarly journals Studying the harmlessness of triolact in experiment on white rats

2019 ◽  
pp. 55-58
Author(s):  
S. V. Shabunin ◽  
G. A. Vostroilova ◽  
P. A. Parshin ◽  
A. A. Korchagina ◽  
N. A. Grigorieva ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Clinical Status ◽  
Recovery Period ◽  
Intragastric Administration ◽  
Animal Blood ◽  
Toxic Dose ◽  
White Rats ◽  
Morphological Picture ◽  
Negative Effect

The aim of the research was to study the effect of the complex antimicrobial drug Triolact on blood morphological and biochemical parameters of white rats in a subacute experiment. It was established in the course of the experiment that the dose of 500.0 mg/kg is the threshold dose, and 100.0 mg/kg is the maximum no-effect dose. Triolact does not have a negative effect on the clinical status, morphological and biochemical parameters of animal blood  in case of repeated intragastric administration for 14 days in doses of 100.0 mg/kg and 250.0 mg/kg both during the application of the drug and in the recovery period. The changes caused by the dose of 500.0 mg/kg are reversible (increase of α-globulin level by 6.9%, creatinine - by 22.9% and urea - by 15.1%), as these indicators in experienced animals returned to normal within the 10 days recovery period. It can be concluded that the  determination of the toxic dose in the format of the present experiment was failed, taking into account the morphometric analysis, macro- and microscopic examination of organs, and studying the morphological picture of blood and biochemical parameters.

scholarly journals Acute Toxicity Ethanol Extract of Bidara Leaves (Ziziphus spina-christi L.) Against Liver and Kidney Function of White Rats

2020 ◽  
Vol 1 (1) ◽  
pp. 17-23
Author(s):  
Nur Syamsi Dhuha ◽  
Haeria ◽  
Hardyanti Eka Putri
Keyword(s):  
Acute Toxicity ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Control Group ◽  
Toxic Dose ◽  
White Rats ◽  
Liver And Kidney ◽  
Anti Fungal ◽  
Actinic Radiation

Bidara plants have the efficacy as antioxidants, anti-inflammatory,antimicrobial, anti-fungal and prevents tumours. Bidara is efficacious to protecthuman DNA cells caused by damage from actinic radiation. This study aims toexplore the acute toxicity test of the ethanol extract of Bidara leaves using whitemice as experimental animals. A total of 30 white rats (Rattus norvegicus) Wistarstrain obtained from the Eureka Research Laboratory (Palembang, Indonesia)weighing between 200 - 250 grams. After one week of adaptation, the mice wererandomly divided into the following six groups, each containing five animals:Normal control group and Bidara extract group (50mg/kg BW; 150 mg/kg BW;450 mg/kg BW; 1350 mg/kg BW; and 4050 mg/kg BW). This study shows thatthe extract of Bidara leaves has a relatively high toxic dose, namely at a dose of4050 mg/kg BW. Bidara leaf extract at doses below 1500 mg/kg BW, shows notoxic effect on the liver. In conclusion, bidara leaf extract has a toxic dose above4000 mg/kg BW in Wistar white rats.

scholarly journals Characterization and Antioxidant Potential Albumin Protein of Canna striata Fish to Rattus Novergicus Induced by Isoniazid Toxic Dose

2018 ◽  
Vol 14 (1) ◽  
pp. 21
Author(s):  
Noer Kumala Indahsari
Keyword(s):  
Free Radicals ◽  
Disulfide Bonds ◽  
Sodium Dodecyl ◽  
Thiol Group ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Protein Extract ◽  
Toxic Dose ◽  
White Rats

Abstract: The Canna Striata fish is a potent source of albumin as an antioxidant. Albumin is a protein capable of binding to free radicals in plasma and has 17 disulfide bonds linking sulfur-containing amino acids. The existence of thiol group bonds in albumin extract of Canna Striata fish is possible to bind to diphenylphycril hydrazyl (DPPH), causing the antioxidant capacity of Canna Striata fish extract to be high. The accumulation of free radicals produced by the administration of antituberculosis drugs from the results of isoniazid metabolism can cause hepatic necrosis. So it requires antioxidants that can reduce free radical levels, which in this study was tested in white rats induced with isoniazid and then treated with albumin protein extract Canna Striata fish. This research method is the first experimental method done is the characterization of albumin protein extract Canna Striata fish with temperature 25˚C and 40˚C by Sodium Dodecyl Sulfate Polyacrilamide Gel Electrophoresis (SDS-PAGE). Then the antioxidant level was tested by measuring the levels of Malondialdehyde (MDA) after 2 last week treatment period in 5 groups of white rats are positive control group, negative control group and 3 treatment groups induced Isoniazid toxic dose are 37.8 mg accompanied by giving of albumin protein extract Canna Striata fish with content of 20ml/kg, 40 ml/kg and 60 ml/kg. The result of this study showed that there were ten albumin protein profiles with size 15,41 kDa, 19,74 kDa 28,6 kDa, 38,87 kDa, 46,08 kDa, 72,45 kDa, 94,42 kDa , 120.82 kDa, 142.80 kDa, and 155.97 kDa. Based on One Way ANOVA and Post Hoc Tukey statistic test, it were found that there was the significant influence and differentiation of Malondialdehyde (MDA) level on giving of albumin  protein extract in white rats induced by Isoniazid with p-value 0,002. The most effective decrease in MDA levels is high levels of albumin protein extract of 60 ml/kg. Keywords: Albumin Protein Extract of Canna Striata, Malondialdehyde, Isoniazid

scholarly journals The Potensi Ekstrak Daun Kelor sebagai Hepatoprotektor terhadap Hepatotoksisitas Akibat Induksi Parasetamol

2019 ◽  
Vol 8 (2) ◽  
pp. 95-100
Author(s):  
Khalisah Nurjihany Salsabila ◽  
Ester Krisdayanti
Keyword(s):  
Liver Damage ◽  
Leaf Extract ◽  
Chemical Compounds ◽  
Google Scholar ◽  
Toxic Dose ◽  
Clinical Complications ◽  
White Rats ◽  
High Doses ◽  
Paracetamol Toxicity ◽  
Therapeutic Doses

Parasetamol merupakan anti-piretik yang paling umum digunakan dan bisa didapat secara bebas. Parasetamol tidak beracun jika diberikan dalam dosis terapi, namun jika diberikan dalam dosis tinggi secara tunggal maupun berulang parasetamol dapat menyebabkan efektoksik. Toksisitas parasetamol merupakan salah satu penyebab keracunan paling umum didunia dan dapat menyebabkan kerusakan hepar bahkan kematian. Tanaman kelor atau Moringaoleifera merupakan tanaman yang mengandung berbagai senyawa kimia bermanfaat dan dapat digunakan sebagai pengobatan alternatif. Studi sebelumnya melaporkan tanaman kelor memiliki aktivitas yang luar biasa terhadap berbagai komplikasi klinis termasuk hepatotoksisitas. Salah satu senyawa kimia yang terdapat pada tanaman kelor adalah flavonoid yang dapat menangkap radikal bebas penyebab kerusakan hepar. Artikel review ini bertujuan untuk menjelaskan gambaran  efek hepatoprotektor pada ekstrak daun kelor terhadap kerusakan hepar karena induksi parasetamol dosis toksis pada penelitian sebelumnya. Metode yang digunakan pada artikel ini ialah dengan melakukan penelusuran artikel melalui database NCBI dan Google Scholar dari tahun 2001 sampai dengan tahun 2019. Tema dalam artikel yang ditelusuri yaitu terkait gambaran efek hepatoprotektor tanaman kelor terhadap hepatotoksisitas karena induksi parasetamol. Hasil dari sintesa artikel yang telah ditemukan yaitu ekstrak daun kelor dapat berpotensi sebagai hepatoprotektor dengan menurunkan kadar SGOT dan SGPT pada tikus putih yang diinduksi parasetamol dosis toksis.   Kata Kunci: ekstrak daun kelor, hepatoprotektor   POTENTIAL EXTRACT OF MORINGAOLEIFERA AS HEPATOPROTECTOR TO PARACETAMOL-INDUCED HEPATOTOXICITY   ABSTRACT Paracetamol is the most commonly used as anti-pyretic and is freely available.paracetamolis not toxic if given in therapeutic doses, but if given in high doses alone or repeatedly it can cause toxic effects. Paracetamol toxicity is one of the most common causes of poisoning in the world and can cause liver damage even death. Moringaoleifera is a plant that contains a variety of beneficial chemical compounds and can be used as an alternative treatment. Previous studies reported that Moringa plants have extraordinary activity against various clinical complications including hepatotoxicity. One of the chemical compounds found in Moringa plants is flavonoids that can capture free radicals that cause liver damage. This review article aims to explain the effect of hepatoprotector ofMoringa leaf extract on paracetamol-induced hepatotoxicity in previous studies. The method used in this article is  searching for articles through the NCBI and Google Scholar databases from 2001 to 2019. Theme in the article is the description of the effects of Moringa plant hepatoprotectors on paracetamol-induced hepatotoxicity. The results of the synthesis of the article found that Moringa leaf extract potentially be a hepatoprotector by reducing SGOT and SGPT levels in white rats induced by toxic dose paracetamol.   Keywords: moringaoleifera  extract, hepatoprotector

Progress report on 21-cm observations at low latitude between longitudes (l 11) 0° and 66°

1967 ◽  
Vol 31 ◽  
pp. 177-179
Author(s):  
W. W. Shane
Keyword(s):  
Preliminary Report ◽  
Progress Report ◽  
Galactic Centre ◽  
Low Latitude ◽  
The Third ◽  
Introductory Lecture ◽  
Centre Region

In the course of several 21-cm observing programmes being carried out by the Leiden Observatory with the 25-meter telescope at Dwingeloo, a fairly complete, though inhomogeneous, survey of the regionl11= 0° to 66° at low galactic latitudes is becoming available. The essential data on this survey are presented in Table 1. Oort (1967) has given a preliminary report on the first and third investigations. The third is discussed briefly by Kerr in his introductory lecture on the galactic centre region (Paper 42). Burton (1966) has published provisional results of the fifth investigation, and I have discussed the sixth in Paper 19. All of the observations listed in the table have been completed, but we plan to extend investigation 3 to a much finer grid of positions.

Preliminary Report of a Comparative Study of Seminiferous Tubular Epithelium from Rats Flown on Cosmos 1887 and SL3

1988 ◽  
Vol 46 ◽  
pp. 276-277
Author(s):  
Walter J. Sapp ◽  
D.E. Philpott ◽  
C.S. Williams ◽  
K. Kato ◽  
J. Stevenson ◽  
...  
Keyword(s):  
Space Flight ◽  
Physiological Response ◽  
Preliminary Report ◽  
Complete Recovery ◽  
Environmental Constraints ◽  
Tubular Epithelium ◽  
Testicular Weight ◽  
Testicular Morphology ◽  
Normal Offspring ◽  
Rats And Mice

Space flight, with its unique environmental constraints such as immobilization, decreased and increased pressures, and radiation, is known to affect testicular morphology and spermatogenesis. Selye, summarized the manifestations of physiological response to nonspecific stress and he pointed out that atrophy of the gonads always occurred. Reports of data collected from two dogs flown in space for 22 days (Cosmos 110) indicate that there was an increase of 30 to 70% atypical spermatozoa when compared to ground based controls. Seventy-five days after the flight the abnormalities had decreased to the high normal value of 30% and mating of these dogs after this period produced normal offspring, suggesting complete recovery. Effects of immobilization and increased gravity were investigated by spinning rats and mice at 2x g for 8-9 weeks. A decrease in testicular weight was noted in spun animals when compared to controls. Immobilization has been show to cause arrest of spermatogenesis in Macaca meminstrins.

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