Lubricin as a Surface Treatment to Reduce Post-operative Biofouling and Infection

2012 ◽  
Vol 1486 ◽  
Author(s):  
George E. Aninwene ◽  
Doug Hall ◽  
Amy Mei ◽  
Gregory D. Jay ◽  
Thomas J. Webster
Keyword(s):  
Surface Modification ◽  
Synovial Fluid ◽  
Bacterial Infection ◽  
Bacterial Adhesion ◽  
Surface Coating ◽  
Polymer Surfaces ◽  
Intraocular Lenses ◽  
Tissue Cultures ◽  
Bacteria Growth ◽  
Adhesive Protein

ABSTRACTThe goal of this research was to investigate the ability of lubricin to prevent bio-fouling of intraocular lenses after surgery, through surface coating trials with lubricin and analogues of the two major sub-units of the lubricin molecule (mucin and vitronectin). Yearly, there are over 6 million surgeries worldwide that involve intraocular lenses (IOLs) 1. However, preventing post-operative biofouling and bacterial infection of these implants remains a challenge 2. Surface modification of IOLs may provide a solution. This study proposes the use of the anti-adhesive protein lubricin (LUB), a glycoprotein found in the synovial fluid, as a means to make polymer surfaces less prone to bacterial adhesion and proliferation; thus, reducing the opportunity for post-operative infection 3. This study used extended bacteria growth trials on tissue cultures polystyrene coated with either lubricin, vitronectin, or mucin to investigate how lubricin and protein sub-regions of lubricin may reduce bacterial adhesion and proliferation.

Lubricin as a Novel Protein Coating to Prevent Bacterial Biofouling

2012 ◽  
Vol 1417 ◽  
Author(s):  
George E. Aninwene ◽  
Erik Taylor ◽  
Douglas Hall ◽  
Amy Mei ◽  
Gregory D. Jay ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Polymer Surface ◽  
Major Complication ◽  
Polymer Surfaces ◽  
Intraocular Lenses ◽  
Exponential Growth Phase ◽  
Bacteria Growth ◽  
Bacterial Proliferation ◽  
Wide Range ◽  
First Time

ABSTRACTYearly, there are over six million cataract surgeries worldwide that involve intraocular lenses (IOLs) [1]. However, preventing post-operative biofouling of these lenses remains a challenge. One major complication is post-operative bacterial infection [2]. Surface modification of IOLs may provide a solution. This study proposes the use of the anti-adhesive protein lubricin (LUB), a glycoprotein found in the synovial fluid, as a means to make polymer surfaces less prone to bacterial adhesion and proliferation, thus reducing the opportunity for post-operative infection [3]. This study used extended bacteria growth trials in the presence of lubricin, vitronectin, and mucin to investigate how lubricin and protein sub-regions of lubricin reduce bacterial functions. This study showed for the first time that polymer surface coatings of lubricin and vitronectin significantly reduce Staphylococcus aureus growth over the course of 15 hours, while mucin was only able to delay the start of the Staphylococcus aureus exponential growth phase and retard proliferation. In solution, both lubricin and mucin significantly reduce bacterial proliferation. Thus, the results of this study demonstrated that lubricin and its sub-regions mucin and vitronectin should be studied for a wide range of antibacterial applications.

scholarly journals Influence of a Point-to-Plane DC Negative Corona Discharge on Gel Surfaces

10.14311/1033 ◽  
2008 ◽  
Vol 48 (4) ◽  
Author(s):  
Y. Klenko ◽  
V. Scholtz
Keyword(s):  
Experimental Investigation ◽  
Surface Modification ◽  
Electric Field ◽  
Corona Discharge ◽  
Biomedical Applications ◽  
Single Point ◽  
Polymer Surfaces ◽  
Negative Corona ◽  
Negative Corona Discharge ◽  
Time Periods

Point-to-plane corona discharge is widely used for modifying polymer surfaces for biomedical applications and for sterilization and decontamination. This paper focuses on an experimental investigation of the influence of the single-point and multi-point corona discharge electric field on gel surface. Three types of gelatinous agar were used as the gel medium: blood agar, nutrient agar and Endo agar. The gel surface modification was studied for various time periods and discharge currents. 

Bioactive poly(ε-caprolactone) microspheres with tunable open pores as microcarriers for tissue regeneration

2019 ◽  
Vol 33 (9) ◽  
pp. 1242-1251 ◽  
Author(s):  
Anmin Zhou ◽  
Zhaoyang Ye ◽  
Yan Zhou ◽  
Wen-song Tan
Keyword(s):  
Cell Culture ◽  
Surface Modification ◽  
Porous Structure ◽  
Pore Structure ◽  
Tissue Regeneration ◽  
Alkaline Hydrolysis ◽  
Surface Coating ◽  
Human Fibroblasts ◽  
Porous Poly ◽  
Critical Challenge

Microparticles with porous structure can be applied as microcarriers for both cell culture and tissue regeneration. While well-controlled pore structure represents a critical challenge to be achieved. In the present study, in order to develop microcarriers for cell culture, a series of poly(ε-caprolactone) microspheres were fabricated with varied macroporous structures. Poly(ε-caprolactone) microspheres were prepared via the integration of the emulsion/solvent evaporation and particle leaching mechanisms. Particularly, by adjusting poly(ε-caprolactone) concentration and the ratio between the porogen paraffin and poly(ε-caprolactone), the microspheres with the pore size of 25.6–84.0 μm and the porosity of 57.4–75.5% were obtained. Further, the microspheres were subjected to alkaline hydrolysis, followed by surface coating with hydroxyapatite. These porous poly(ε-caprolactone) microspheres with surface modification well supported the adhesion and growth of human fibroblasts. Together, bioactive poly(ε-caprolactone) microspheres with controlled pore structure are potential to be applied in cell culture and tissue regeneration.

scholarly journals Exposure of Smaller and Oxidized Graphene on Polyurethane Surface Improves its Antimicrobial Performance

Nanomaterials ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 349 ◽  
Author(s):  
Inês Borges ◽  
Patrícia C. Henriques ◽  
Rita N. Gomes ◽  
Artur M. Pinto ◽  
Manuel Pestana ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Bacterial Adhesion ◽  
Antibacterial Effect ◽  
Composite Layer ◽  
Dip Coating ◽  
Homogeneous Layer ◽  
Melt Blending ◽  
Bacteria Growth ◽  
Antimicrobial Performance ◽  
Antibacterial Potential

Catheter-related infections are a common worldwide health problem, highlighting the need for antimicrobial catheters. Here, antibacterial potential of graphene nanoplatelets (GNP) incorporated in the commonly used polymer for catheter manufacture—polyurethane (PU)—is investigated. Two strategies are explored: melt-blending, producing a composite, and dip coating, where a composite layer is deposited on top of PU. GNP with different lateral sizes and oxidation degrees—GNP-M5, GNP-M15, GNP-M5ox, GNP-M15ox—are applied in both strategies, and the antimicrobial potential towards Staphylococcus epidermidis of GNP dispersions and GNP-containing PU evaluated. As dispersions, oxidized and smaller GNP powders (GNP-M5ox) inhibit 74% bacteria growth at 128 µg/mL. As surfaces, GNP exposure strongly impacts their antimicrobial profile: GNP absence at the surface of composites yields no significant effects on bacteria, while by varying GNP: PU ratio and GNP concentration, coatings enhance GNP exposure, depicting an antimicrobial profile. Oxidized GNP-containing coatings induce higher antibacterial effect than non-oxidized forms, particularly with smaller GNPox, where a homogeneous layer of fused platelets is formed on PU, leading to 70% reduction in bacterial adhesion and 70% bacterial death. This pioneering work unravels how to turn a polymer clinically used to produce catheters into an antimicrobial surface, crucial to reducing risk of infection associated with catheterization.

Visible-light initiated polymerization of dopamine in a neutral environment for surface coating and visual protein detection

2018 ◽  
Vol 9 (42) ◽  
pp. 5242-5247 ◽  
Author(s):  
Jinhu Wang ◽  
Guolu Ma ◽  
Wei Huang ◽  
Yi He
Keyword(s):  
Surface Modification ◽  
Visible Light ◽  
Surface Coating ◽  
Protein Detection ◽  
Promising Avenue

Mussel-inspired polydopamine (PDA) coating is a promising avenue for surface modification.

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