Platform based Detection Technologies from Micro scale to Nanoscale

2006 ◽  
Vol 915 ◽  
Author(s):  
Vindhya Kundura ◽  
Sudhaprasanna Kumar Padigi ◽  
Shalini Prasad
Keyword(s):  
Rapid Identification ◽  
Electrical Signal ◽  
Label Free ◽  
C Reactive Protein ◽  
Protein Biomarkers ◽  
Reactive Protein ◽  
Non Invasive ◽  
Binding Event ◽  
High Throughput Detection ◽  
Detection Technologies

AbstractRapid, multiplexed, high throughput detection of proteins is essential for the development of protein biomarkers as sensors. Electrical alignment and detection is a non-invasive, label free technique for rapid identification of bimolecular. We present here a micro fabricated platform based detector for rapidly identifying protein biomarkers present in atherosclerotic plaque for rapid clinical diagnosis of arterial obstruction. This is achieved by electrical assembly of polystyrene beads functionalized with specific antibody receptors (anti-C-reactive protein) .The electrical assembly is achieved using electrophoresis. The polystyrene “bridge” micro structure formed due to electrical assembly aids in the amplification of the antibody-antigen binding event. Antigen (C-reactive protein) at nanogram / ml concentration was detected when binding of the antigen resulted in an amplification of the electrical signal that was measured from the base microelectrode platform. This technique is a demonstration of the application of microscale technology (electrodes) in nanoscale (protein) electrical detection.

scholarly journals Demonstration of a Label-Free and Low-Cost Optical Cavity-Based Biosensor Using Streptavidin and C-Reactive Protein

Biosensors ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 4
Author(s):  
Donggee Rho ◽  
Seunghyun Kim
Keyword(s):  
Limit Of Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Optical Cavity ◽  
Sample Volume ◽  
Small Sample ◽  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Cavity Structure

An optical cavity-based biosensor (OCB) has been developed for point-of-care (POC) applications. This label-free biosensor employs low-cost components and simple fabrication processes to lower the overall cost while achieving high sensitivity using a differential detection method. To experimentally demonstrate its limit of detection (LOD), we conducted biosensing experiments with streptavidin and C-reactive protein (CRP). The optical cavity structure was optimized further for better sensitivity and easier fluid control. We utilized the polymer swelling property to fine-tune the optical cavity width, which significantly improved the success rate to produce measurable samples. Four different concentrations of streptavidin were tested in triplicate, and the LOD of the OCB was determined to be 1.35 nM. The OCB also successfully detected three different concentrations of human CRP using biotinylated CRP antibody. The LOD for CRP detection was 377 pM. All measurements were done using a small sample volume of 15 µL within 30 min. By reducing the sensing area, improving the functionalization and passivation processes, and increasing the sample volume, the LOD of the OCB are estimated to be reduced further to the femto-molar range. Overall, the demonstrated capability of the OCB in the present work shows great potential to be used as a promising POC biosensor.

Label-free RNA aptamer-based capacitive biosensor for the detection of C-reactive protein

2010 ◽  
Vol 12 (32) ◽  
pp. 9176 ◽  
Author(s):  
Anjum Qureshi ◽  
Yasar Gurbuz ◽  
Saravan Kallempudi ◽  
Javed H. Niazi
Keyword(s):  
Rna Aptamer ◽  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Capacitive Biosensor

Label-free detection of C-reactive protein using reflectometric interference spectroscopy-based sensing system

2012 ◽  
Vol 728 ◽  
pp. 64-68 ◽  
Author(s):  
Hyung Woo Choi ◽  
Yasuhiko Sakata ◽  
Yoshikazu Kurihara ◽  
Tooru Ooya ◽  
Toshifumi Takeuchi
Keyword(s):  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Sensing System ◽  
Reflectometric Interference Spectroscopy ◽  
Label Free Detection

Label-free detection of C-Reactive protein using bioresponsive hydrogel-based surface relief diffraction gratings

2021 ◽  
pp. 113561
Author(s):  
María Isabel Lucío ◽  
Andy Hernández Montoto ◽  
Estrella Fernández ◽  
Sabri Alamri ◽  
Tim Kunze ◽  
...  
Keyword(s):  
Surface Relief ◽  
Diffraction Gratings ◽  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Label Free Detection

Serum C-reactive protein: A non-invasive marker of alcoholic hepatitis

2006 ◽  
Vol 41 (12) ◽  
pp. 1473-1479 ◽  
Author(s):  
Geoffroy Vanbiervliet ◽  
Frédèrique Le Breton ◽  
Maria-Alessandra Rosenthal-Allieri ◽  
Eve Gelsi ◽  
Eugenia Marine-Barjoan ◽  
...  
Keyword(s):  
Alcoholic Hepatitis ◽  
Protein A ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Non Invasive

scholarly journals Influence of Selected Biomarkers on Stress and Alexithymia in Patients under Hemodialysis Treatment

2019 ◽  
Author(s):  
Esra Tayaz ◽  
Ayşegül Koç
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Demographic Data ◽  
High Stress ◽  
Chronic Kidney Failure ◽  
C Reactive Protein ◽  
Protein Biomarkers ◽  
Reactive Protein ◽  
Hemodialysis Treatment ◽  
Data Form

Abstract Background: Chronic renal failure causes a number of physical problems in patients. Hemodialysis treatment and the stress brought along by the treatment are high and this circumstance sets the ground for alexithymia. Alexithymic feelings basically emerge as restriction in the world of emotion and thought, and inability to recognize physiological changes. Biomarkers that are indicators of physical change are influential in the stress lives of individuals. They lead to negative changes in the physical and mental lives of patients who have chronic kidney failure and individuals who receive hemodialysis treatment. This research was carried out as a relationship seeker in order to determine the influence of urea, creatinin, sodium, potassium, hemoglobin, hematocrit, albumin, calcium, phosphorus and C-reactive protein biomarkers on stress and alexithymia in individuals, who are diagnosed with chronic renal failure and receive hemodialysis treatment. Methods: The research environment was formed of patients who underwent hemodialysis treatment in a hospital in Turkey. The subject group was completed of 72 individuals. Demographic data form, biochemical data form, Hemodialysis Stressor Scale and Toronto Alexithymia Scale were used in the research. Results: It was found that the levels of perceived stress of individuals who participated in the research were high at all dimensions, and 59.7% were alexithymic. The means of the total scale scores of all patients were calculated as 87.81±13.59 for HSS and 62.46±9.84 for TAS. The relationship between TAS-20 and HSS and selected biomarkers were determined (p<0.05). Conclusion: It was concluded that stress and alexithymic feelings were high in patients who received hemodialysis treatment. It was concluded that there is a relationship between C-reactive protein, creatinin, sodium, hemoglobin, hematocrit, potassium from the biomarkers and the scales and scale sub-dimensions. It is necessary to increase the awareness of nurses on the importance of the skills to communicate with individuals who have to cope with stress, manage emotions, and have high stress and emotional deprivation.

Rapid C-reactive protein determination in whole blood with a White Light Reflectance Spectroscopy label-free immunosensor for Point-of-Care applications

2018 ◽  
Vol 260 ◽  
pp. 282-288 ◽  
Author(s):  
Georgios Koukouvinos ◽  
Dimitrios Goustouridis ◽  
Konstantinos Misiakos ◽  
Sotirios Kakabakos ◽  
Ioannis Raptis ◽  
...  
Keyword(s):  
White Light ◽  
Whole Blood ◽  
Point Of Care ◽  
Reflectance Spectroscopy ◽  
Label Free ◽  
C Reactive Protein ◽  
Protein Determination ◽  
Reactive Protein ◽  
Light Reflectance

scholarly journals High-flow nasal cannula oxygen therapy to treat patients with hypoxemic acute respiratory failure consequent to SARS-CoV-2 infection

Thorax ◽  
2020 ◽  
Vol 75 (11) ◽  
pp. 998-1000 ◽  
Author(s):  
Andrea Vianello ◽  
Giovanna Arcaro ◽  
Beatrice Molena ◽  
Cristian Turato ◽  
Andi Sukthi ◽  
...  
Keyword(s):  
Respiratory Failure ◽  
Acute Respiratory Failure ◽  
High Flow ◽  
Nasal Cannula ◽  
High Flow Nasal Cannula ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Non Invasive ◽  
Non Invasive Ventilation ◽  
Hypoxemic Acute Respiratory Failure

This observational study aims to assess the outcome and safety of O2-therapy by high-flow nasal cannula (HFNC) in 28 consecutive patients with severe hypoxemic acute respiratory failure (hARF) consequent to SARS-CoV-2 infection, unresponsive to conventional O2-therapy. Nineteen patients had a positive response. Nine patients required escalation of treatment to non-invasive ventilation (five subsequently intubated). None of the staff had a positive swab testing during the study period and the following 14 days. Severity of hypoxemia and C reactive protein level were correlated with HFNC failure. These data suggest HFNC to be a safe treatment for less severe patients with SARS-CoV-2 hARF and efficacy will need to be assessed as part of a clinical trial.

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