Human Bone Marrow Stem Cell Responses on Electrospun Bombyx mori Silk Fibroin

2002 ◽  
Vol 735 ◽  
Author(s):  
Hyoung-Joon Jin ◽  
Jingsong Chen ◽  
Vassilis Karageorgiou ◽  
Gregory H. Altman ◽  
David L. Kaplan
Keyword(s):  
Bone Marrow ◽  
Bombyx Mori ◽  
Silk Fibroin ◽  
High Performance ◽  
High Surface ◽  
High Surface Area ◽  
Human Bone ◽  
Wound Dressings ◽  
Human Bone Marrow ◽  
Biomaterial Scaffolds

ABSTRACTElectrospinning for the formation of nanoscale diameter fibers has been explored for high performance filters and biomaterial scaffolds for vascular grafts or wound dressings. Fibers with nanoscale diameters provide benefits due to high surface area. In this study we used electrospinning for protein-based biomaterials to fabricate scaffolds from aqueous regenerated silkworm silk, Bombyx mori,. Adhesion, spreading and proliferation of human bone marrow stem cells (hBMSCs) on electrospun silk was characterized. Scanning electron microscopy (SEM) and MTT analyses demonstrated that the electrospun silk matrices promoted hBMSC attachment and proliferation over 10 days in culture. The responses of the hBMSCs on the electrospun silk matrices, combined with the biocompatibile properties of the silk fibroin protein matrix, suggest potential for use of this biomaterial as scaffolds for tissue engineering.

Antheraea pernyi silk fibroin maintains the immunosupressive properties of human bone marrow mesenchymal stem cells

2009 ◽  
Vol 33 (11) ◽  
pp. 1127-1134 ◽  
Author(s):  
Xi-Ying Luan ◽  
Guan-Hua Huo ◽  
Ming-Zhong Li ◽  
Shen-Zhou Lu ◽  
Xue-Guang Zhang
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Silk Fibroin ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Antheraea Pernyi ◽  
Marrow Mesenchymal Stem Cells

The structure and property of the electrospinning silk fibroin/gelatin blend nanofibers

e-Polymers ◽  
2008 ◽  
Vol 8 (1) ◽  
Author(s):  
Bao Weiwei ◽  
Zhang Youzhu ◽  
Yin Guibo ◽  
Wu Jialin
Keyword(s):  
Silk Fibroin ◽  
Biomedical Applications ◽  
Electric Field Intensity ◽  
Pore Diameter ◽  
High Surface ◽  
High Surface Area ◽  
Structure And Property ◽  
Biomaterial Scaffolds ◽  
Β Sheets ◽  
Scanning Electron

AbstractNanofibers could provide benefits due to high surface area for biomaterial scaffolds. In order to improve both silk fibroin and gelatin performance for potential biomedical applications, in this article the electrospinning of silk fibroin (SF)/gelatin (GT) blends with different composition ratio and concentration was performed with formic acid as a spinning solvent. The nanofibers morphologies and diameters were observed by Scanning electron microscopy (SEM). Blending with GT could improve the viscosity and spinnability of spinning solution. As-spun SF/GT blend nanofibers were more homogeneous and non-beaded than pure SF nanofibers. The diameter decreased with higher voltage at same electric field intensity. On increasing the concentrations of spinning solution, the pore diameter of nanofibers decreased, and the porosity increased. In addition, FTIR and DTA analyses showed SF/GT nanofibers had more β-sheets than pure SF nanofibers, which may be caused by an intermolecular interaction between SF and GT. Blending with GT also led to substantial enhancement in mechanical properties of SF nanofibers.

scholarly journals Engineering adipose-like tissue in vitro and in vivo utilizing human bone marrow and adipose-derived mesenchymal stem cells with silk fibroin 3D scaffolds

Biomaterials ◽  
2007 ◽  
Vol 28 (35) ◽  
pp. 5280-5290 ◽  
Author(s):  
Joshua R. Mauney ◽  
Trang Nguyen ◽  
Kelly Gillen ◽  
Carl Kirker-Head ◽  
Jeffrey M. Gimble ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Silk Fibroin ◽  
Human Bone ◽  
Human Bone Marrow ◽  
3D Scaffolds

Attachment and growth of human bone marrow derived mesenchymal stem cells on regenerated antheraea pernyi silk fibroin films

2006 ◽  
Vol 1 (4) ◽  
pp. 181-187 ◽  
Author(s):  
Xi-Ying Luan ◽  
Yong Wang ◽  
Xiang Duan ◽  
Qiao-Yan Duan ◽  
Ming-Zhong Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Silk Fibroin ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Antheraea Pernyi

scholarly journals Silver Nanoparticle-Coated Polyhydroxyalkanoate Based Electrospun Fibers for Wound Dressing Applications

Materials ◽  
2021 ◽  
Vol 14 (17) ◽  
pp. 4907
Author(s):  
Ozlem Ipek Kalaoglu-Altan ◽  
Havva Baskan ◽  
Timo Meireman ◽  
Pooja Basnett ◽  
Bahareh Azimi ◽  
...  
Keyword(s):  
Wound Dressing ◽  
High Performance ◽  
High Surface ◽  
High Surface Area ◽  
Human Keratinocytes ◽  
Dip Coating ◽  
Wound Dressings ◽  
Coating Method ◽  
Dip Coating Method

Wound dressings are high performance and high value products which can improve the regeneration of damaged skin. In these products, bioresorption and biocompatibility play a key role. The aim of this study is to provide progress in this area via nanofabrication and antimicrobial natural materials. Polyhydroxyalkanoates (PHAs) are a bio-based family of polymers that possess high biocompatibility and skin regenerative properties. In this study, a blend of poly(3-hydroxybutyrate) (P(3HB)) and poly(3-hydroxyoctanoate-co-3-hydroxy decanoate) (P(3HO-co-3HD)) was electrospun into P(3HB))/P(3HO-co-3HD) nanofibers to obtain materials with a high surface area and good handling performance. The nanofibers were then modified with silver nanoparticles (AgNPs) via the dip-coating method. The silver-containing nanofiber meshes showed good cytocompatibility and interesting immunomodulatory properties in vitro, together with the capability of stimulating the human beta defensin 2 and cytokeratin expression in human keratinocytes (HaCaT cells), which makes them promising materials for wound dressing applications.

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