Optimization of prevalual preparation of endometry before the treatment аrt methods in patients with tubal-periotional infertility by application of the injection-screetching procedure

2018 ◽  
pp. 87-91
Author(s):  
L.V. Suslikova ◽  
◽  
A.V. Serbenuyk ◽  
Keyword(s):  
Assisted Reproductive Technologies ◽  
Basal Layer ◽  
Reproductive Technologies ◽  
Follicular Phase ◽  
Optimal Time ◽  
Repeated Cycle ◽  
Endometrial Scratching ◽  
Group A ◽  
Group B ◽  
Control Stimulation

The article presents an analysis of literature data and the results of own prospective studies of the application of the local iatrogenic injury method with the parallel introduction of plasma enriched with platelets into the basal layer of the endometrium in pregravid preparation for treatment with assisted reproductive technologies (ART) in patients with tubal peritoneal infertility and marked lag development of the endometrium. The objective: was to determine the optimal time for the injection of scratch scratching in pregravid preparation before the next cycle of ART treatment in patients with tubal peritoneal infertility and marked lag in the development of the endometrium. Materials and methods. The study included 109 patients with unsuccessful attempts to treat ART with a tubal peritoneal factor of infertility with a marked degree of lag in the development of the endometrium according to absolute indications. The subjects were divided into three groups. Group A – 47 patients who experienced a marked lag in the development of the endometrium during the control stimulation of superovulation during ART (ETC + ET) and suggested the application of the procedure of injection scaling of the endometrium on the 7th day of the stimulation cycle (CA). Group B – 32 patients who underwent the procedure of injection endometrial scratching on the 22nd day of the menstrual cycle (MC), which preceded the repeated cycle of CGI + ET. Group B – 30 patients who underwent the procedure of injection endometrial scratching on the 8th day of the MC, which preceded the repeated cycle of CCN + ET. Results. The use of injection scratches is more effective in improving the clinical outcomes of ART by using the methods of ART (CAS + ET) in the case of a pronounced delay in the development of the endometrium in the follicular phase of the CA on the 6th to 7th day of gonadotropin use. During treatment with ART, the frequency of pregnancy for each transfer of embryos (CHNBPE) was significantly lower, amounting to 9.4% (3 of 32) in group B and 7.1% (2 of 28) in group B than CHNBPE in group A – 27.7% (13 of 47). The frequency of early reproductive losses in group A was 23.1% (3 of 13), in group B – 66.7% (2 of 3), in group B – 50% (1 of 2). Conclusion. During the study of the optimal period for the appointment of injection scratching, it was found that injection endometrial scratching using autoplasma enriched with platelets demonstrates the best pregnancy rate for each transfer of embryos and low early reproductive loss if performed in the follicular phase of the stimulation cycle by 6–7th day of gonadotropins). Key words: infertility, methods of assisted reproductive technology, hypoplasia of the endometrium, hysteroscopy, plasma-riched platelets.

scholarly journals INFLUENCE ON THE OUTCOME OF ART PROGRAMS OF FROZEN-THAWED BLASTOCYSTS EXPANDED ON FIVE OR SIX DAY

2021 ◽  
pp. 68-75
Author(s):  
A.O. Polumiskova ◽  
S.I. Tevkin ◽  
T.M. Jussubaliyeva ◽  
M.S. Shishimorova
Keyword(s):  
Pregnancy Rate ◽  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Clinical Pregnancy ◽  
Clinical Pregnancy Rate ◽  
Miscarriage Rate ◽  
Art Programs ◽  
Group A ◽  
The Difference ◽  
Group B

In order to increase the effectiveness of assisted reproductive technologies (ART) programs, it is essential to improve and develop conditions of embryo culture prior its transfer or cryopreservation of expanded blastocysts on the day 5 or 6. The aim of the study was to assess the effect of human blastocysts’ expansion timing on clinical pregnancy rate (CPR), miscarriage rate (MR) and take-home baby rate (THBR) in frozen-thawed cycles during ART programs. The study involved 2275 frozen embryo transfers (FET) of blastocysts expanded on the day 5 (group A) and 170 FET of blastocysts expanded on the day 6 (group B). The pregnancy rates in both groups were 50.8% and 46.5% respectively. There were no statistically significant differences in clinical pregnancy rate 37.4% and 37.0%, miscarriage rate 26.0% and 21.5% in both groups, respectively. THBR, as the main indicator of efficiency in the programs with transfer of post thawed expanded blastocysts on the day 5 (group A) or 6 (group B) were 36.5% and 35.2%, respectively (the difference is insignificant). In conclusion, in cryoprotocols the day of blastocyst expansion (day 5 or 6 of development) does not statistically affect PR, MR and THBR. In FET programs the quality of blastocyst (excellent and good) should be prioritized regardless of the day of cryopreservation.

322 INDUCTION OF SUPEROVULATION IN PROESTRUS DOGS USING SERUM GONADOTROPIN OF PREGNANT MARES AND HUMAN CHORIONIC GONADOTROPIN

2011 ◽  
Vol 23 (1) ◽  
pp. 257
Author(s):  
K. S. Park ◽  
K. J. Kim ◽  
S. A. Choi ◽  
J. H. Lee ◽  
E. Y. Kim ◽  
...  
Keyword(s):  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Reproductive Physiology ◽  
Control Group ◽  
Complex Nature ◽  
Progesterone Concentration ◽  
Serum Progesterone ◽  
Group A ◽  
Group B

Several factors influence the limited application of assisted reproductive technologies (ART) in the canine species. Most problems arise because of the complex nature of reproductive physiology of the dog. For example, dogs are monoestrus, generally exhibiting oestrus only once every 6 month to 1 year. In the canine species, there has been little research on the ART because of difficulties associated with anatomy and reproductive physiology. Because in vitro maturation of canine oocytes has been particularly difficult, in vivo matured oocytes have been used in somatic cell nuclear transfer. However, the number of oocytes that can be obtained using this approach is limited, with ∼6 to 10 good oocytes being obtained per collection. The present study was undertaken to evaluate the effects of different dosages of eCG on folliculogenesis in the dog and to determine the number of oocytes that might be obtained after ovulation. The experimental design involved 3 groups that were treated with different dosages of eCG at the early stage of proestrus; Group A was a nontreatment (control) group, Group B received 200-IU eCG SC injections every day, and Group C was injected with 500 IU of eCG every 2 days until reaching 2 to 3 ng mL–1 serum progesterone concentration, respectively. Dogs in Groups B and C received a 1 000-IU hCG SC injection when progesterone concentrations reached 2 to 3 ng mL–1. The serum progesterone concentration was examined with a Radioimmunoassay Kit (Diagnostic Systems Laboratories Inc., Webster, TX, USA). The day of ovulation was considered as the day when serum progesterone concentration reached 4.0 to 7.2 ng mL–1. Approximately 70 to 76 h after ovulation, the dogs were subjected to the oocyte collection procedure. One-way ANOVA followed by Duncan’s multiple range tests was performed. The significance level was <0.05. In total, 446 oocytes were recovered from 60 bitches, with an average of 6.4 oocytes/dog in Group A (from 49 bitches), 16.6 oocytes/dog in Group B (from 5 bitches), and 8.5 oocytes/dog in Group C (from 6 bitches). The oocytes collection rate (number of oocytes per dog) in Group B was higher (P < 0.05) than those in the other 2 groups. In conclusion, the results showed that eCG treatment with hCG in early-proestrus-stage bitches can result in a greater number of recovered in vivo matured oocytes. This technology could become a useful research tool for canine cloning and ART.

scholarly journals A Prospective Study Comparing Two-Time Points of Thyroid Hormone Replacement during the Holy Month of Ramadan

2019 ◽  
Vol 2019 ◽  
pp. 1-5 ◽  
Author(s):  
Zeinab Dabbous ◽  
Buthaina Alowainati ◽  
Sara Darwish ◽  
Hamda Ali ◽  
Seleena Farook ◽  
...  
Keyword(s):  
Prospective Study ◽  
Hormone Replacement ◽  
Prospective Trial ◽  
Primary Hypothyroidism ◽  
Optimal Time ◽  
Open Label ◽  
Time Points ◽  
Number Of Patients ◽  
Group A ◽  
Group B

Background. Muslims all over the world fast during the month of Ramadan from dawn until dusk. There is little data regarding the best timing of levothyroxine intake during the month of Ramadan where taking it on an empty stomach represents a challenge to most patients. Our study aims to compare two-time points of levothyroxine intake during Ramadan in terms of change in thyroid stimulating hormone (TSH), compliance, and convenience. Study Design and Methods. This was an open-label, randomized, prospective trial. Adult patients known to have primary hypothyroidism with stable TSH for the last 6 months who intended to fast during the month of Ramadan were invited to participate in this prospective study. The study took place during Ramadan of H1438 (May-June 2017). All patients were randomly assigned to two groups. In group A (n= 50) patients took levothyroxine 30 minutes before breaking the fast at sunset (iftar), and in group B (n= 46) patients took it 30 minutes before an early morning meal before sunrise (suhour). Results. TSH levels increased in both group A (from 1.99 to 3.28 mIU/L) and group B (from 1.54 to 3.28 mIU/L) after Ramadan fasting. There was no difference between the two groups. Compliance with intake instructions, all of the time, was reported in 41.6% of group A and 35.7% of group B patients. In both the groups, 95% of patients said it was convenient for them to take the medication at the assigned time. Conclusion. Choosing an optimal time for levothyroxine intake during the month of Ramadan remains a challenge. The current study did not provide any evidence on ideal time and dose of levothyroxine administration during fasting to manage hypothyroidism. Studies with a larger number of patients need to be done to further explore this issue.

P–254 The outcome of artificial reproductive technologies (ART) cycles with transfer of frozen-thawed blastocysts depending on whether expanded on day 5 or day 6

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
A Polumiskova ◽  
S Tevkin ◽  
M Shishimorova ◽  
T Jussubaliyeva
Keyword(s):  
Pregnancy Rate ◽  
Positive Outcome ◽  
Reproductive Technologies ◽  
Endometrial Receptivity ◽  
Ongoing Pregnancy ◽  
Miscarriage Rate ◽  
Ongoing Pregnancy Rate ◽  
Preimplantation Genetic Testing ◽  
Group A ◽  
Group B

Abstract Study question Is there a difference in ART cycle results after frozen embryo transfer (FET), depending on whether blastocysts were cryopreserved on day 5 or 6? Summary answer There’s no statistical difference in the clinical pregnancy rate (CPR), life birth rate (LBR), miscarriage rate (MR) between embryos frozen on day 5 and 6. What is known already Currently, opinions differ regarding this topic. Previous studies demonstrated no difference in ongoing pregnancy rates between embryos frozen on day 5 (group A) or day 6 (group B) after FET. However, metanalysis (2019) suggested higher CPR and LBR after transferring embryos from group A rather than group B. It has also been established that ovarian stimulation leads to endometrial changes that result in deleterious effects on the implantation window and endometrial receptivity. Consequently, fresh transfers were excluded. Due to hormonal priming of endometrial receptivity, the same pregnancy outcomes should be expected with frozen-thawed blastocysts (day 5 vs 6). Study design, size, duration Retrospective cohort study was conducted between January 2015 and December 2018 with selected group of patients under 40 years of age. Group A consisted of 2275 cryotransfers of blastocyst expanded on day 5; group B included 170 cryotransfers of blastocyst on day 6. Both groups had an average of 1,52 embryos transferred per patient. Participants/materials, setting, methods Embryos were vitrified and warmed with Cryotop method (Kitazato, BioPharma). Blastocysts were scored according to Gardner and Schoolcraft grading system. Only expanded on day or 6 blastocysts of excellent and good (AA, AB, BA, BB) quality were selected. The embryos were cultured in CSC medium (Irvine Scientific) for 2–4 hours prior intrauterine transfer. The cycles with donor gametes, surrogacy and preimplantation genetic testing (PGT) were excluded. Statistical validity was assessed by Pearson’s chi-squared test. Main results and the role of chance The rates of the CPR, the ongoing pregnancy rate (OPR) and the LBR between group A and B were 50,8% (1157/2275) vs 46,5% (79/170) (p = 0,26), 37,4% (852/2275) vs 37,0% (63/170) (p = 0,91), 36,5% (832/2275) vs 35,2% (60/170) (p = 0,73) respectively and no significant differences were found in each category. Moreover, similarly there were no significant differences in the miscarriage rate 26,0% (301/1157) and 21,5% (17/79) (p = 0,37) as well Limitations, reasons for caution The study is limited due to uneven distribution of patients in both groups and by a low number of participants. The grading of blastocysts’ quality is also subjected to a human factor. Wider implications of the findings: This study confirms that frozen-thawed blastocysts do not seem to exhibit a difference in the CPR, OPR, LBR and MR whether they were expanded on day 5 or day 6. The cryopreservation of day 6 blastocyst can increase the chances of the patient for the positive outcome. Trial registration number Not applicable

The clinical significance and optimal timing of postoperative computed tomography following cranial surgery

2010 ◽  
Vol 113 (5) ◽  
pp. 1021-1025 ◽  
Author(s):  
Ahmad Khaldi ◽  
Vikram C. Prabhu ◽  
Douglas E. Anderson ◽  
Thomas C. Origitano
Keyword(s):  
Neurological Deficit ◽  
Ct Scans ◽  
Optimal Time ◽  
Optimal Timing ◽  
Head Ct ◽  
Group A ◽  
Group B ◽  
Unexpected Findings ◽  
Over Time

Object This study was conducted to evaluate the value of postoperative CT scans in determining the probability of return to the operating room (OR) and the optimal time to obtain such scans to determine the effects of surgery. Methods Between January and December 2006 (12 months), all postoperative head CT scans obtained for 3 individual surgeons were reviewed. Scans were divided into 3 groups, which were determined by the preference of each surgeon: Group A (early scans—scheduled between 0 and 7 hours); Group B (delayed scans—scheduled between 8 and 24 hours); and Group C (urgent scans—ordered because of a new neurological deficit). The initial scans were reviewed and analyzed in 2 different fashions. The first was to analyze the efficacy of the scans in predicting return to the OR. The second was to determine the optimal time for obtaining a scan. The second analysis was a review of serial postoperative scans for expected versus unexpected findings and changes in the acuity of these findings over time. Results In 251 (74%) of 338 cases, the patients had postoperative head CT scans within 24 hours of surgery. Analysis 1 determined the percent of patients returning to the OR for emergency treatment based on postoperative scans: Group A (early)—133 patients, with 0% returning to the OR; Group B (delayed)—108 patients, with 0% returning to the OR; and Group C (urgent)—10 patients, with 30% returning to the OR (p < 0.05). Analysis 2 determined the optimal timing of postoperative scans and changes in scan acuity: Group A (early scan) had an 11% incidence of change in acuity on subsequent scans. Group B (delayed scan) had a 3% incidence of change in acuity on follow-up scans (p < 0.05). Conclusions Routine postoperative scans at 0–7 hours or at 8–24 hours are not predictive of return to the OR, whereas patients with a new neurological deficit in the postoperative period have a 30% chance of emergency reoperation based on CT scans. In addition, early postoperative scans (0–7 hours) fail to predict CT changes, which might evolve over time and may influence postoperative medical management.

scholarly journals Progesterone in Assisted Reproduction: Classification, Pharmacology and Its Clinical Coorelation: A Commentary

2020 ◽  
pp. 1-5
Author(s):  
Kunal Rathod
Keyword(s):  
Assisted Reproductive Technologies ◽  
Elevated Serum ◽  
Reproductive Technologies ◽  
Follicular Phase ◽  
The Body ◽  
Successful Outcome ◽  
Ongoing Pregnancy ◽  
Ongoing Pregnancy Rate ◽  
Serum Progesterone ◽  
And Function

The modulating effects of progesterone on endometrium structure and function are the basis for successful outcome in reproductive treatments. Considering this, progesterone has a big role in treatment of infertility and supporting the ongoing pregnancy. In this review article we have attempted to review different forms of progesterone, their metabolism in the body and the role played by exogenous as well as endogenous progesterone in assisted reproductive technologies (ART). Elevated serum progesterone levels at the end of the follicular phase in controlled ovarian stimulation (COS) leads to a poorer ongoing pregnancy rate in IVF cycles due to reduced endometrial receptivity.

scholarly journals Pregnancy and live birth rates in women with endometriosis related infertility in Latvia

2019 ◽  
Vol 8 (3) ◽  
pp. 808
Author(s):  
Jekaterina Avdotina ◽  
Aleksandra Mezecka-Oleinika ◽  
Vija Silina ◽  
Zane Vitina
Keyword(s):  
Laparoscopic Surgery ◽  
Live Birth ◽  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Human Reproduction ◽  
Optimal Time ◽  
First Year ◽  
Vitro Fertilization ◽  
Significant Difference

Background: Women with endometriosis experience painful symptoms and/or infertility, others have no symptoms at all. According to European Society of Human Reproduction and Embryology guidelines, surgery and assisted reproductive technologies (ART) are an appropriate treatment in cases of endometriosis-associated infertility. There are controversial data on the results of surgery and ART in patients with endometriosis.Methods: Retrospective analysis including 99 infertile patients aged between 25 and 48 years old. All of them had laparoscopic surgery as the primary option. 51 of them undergoing in vitro fertilization or intracytoplasmic sperm injection or frozen embryo transfer from 2003 through 2018 at SIA ‘Clinic EGV’.Results: The mean age of women was 34.2±4.5. In 56 (33.5%) cases was only surgery with 28 (50.9%) biochemical pregnancies and 26 (48.1%) live birth. In 111 (66.5%) cases there were surgery with ART with 48 (47.6%) biochemical pregnancies and 23 (22.7%) live birth. In 1 group patients mean age 30.7±4.6 and 2 group with mean age 35.1±4.2 (p=0.000). It was found that there is significant difference between endometriosis phenotype, infertility type, duration of infertility, repeated laparoscopic surgery, ART cycles, retrieved oocyte count and biochemical pregnancy rate.Conclusions: Patients with endometriosis related infertility should undergo surgical treatment as the primary option. Those patients who do not become pregnant after surgery must be treated with assisted reproductive technology. The optimal time to perform ART is first year after endometriosis surgery.

scholarly journals A comparative analysis of the cycle fecundity rates associated with ovarian hyperstimulation along with IUI versus medical management in the treatment of mild to moderate oligozoospermia in males

2021 ◽  
Vol 10 (2) ◽  
pp. 711
Author(s):  
Tanaya Acharyya ◽  
Arun Paul Choudhury
Keyword(s):  
Medical Management ◽  
Sperm Concentration ◽  
Reproductive Technologies ◽  
Normal Serum ◽  
Prospective Clinical Study ◽  
Ovarian Hyperstimulation ◽  
Male Factor ◽  
Fecundity Rate ◽  
Group A ◽  
Group B

Background: To compare the pregnancy rate in infertile couples with mild to moderate oligozoospermia receiving ovarian hyperstimulation and IUI, with that of medical management with CC.Methods: A total 120 couples with males having sperm concentration between 5 to 20 million/ml, normal serum gonadotropins and testosterone, and a presumptively fertile partner were enrolled in this prospective clinical study, during the study period of 19 months, 31st January 2019 till 1st September 2020. 120 couples were divided randomly into two groups A (n=60) and B (n=60). In group A, 60 couples were subdivided equally into 3 groups, each having 20 couples. Ovarian hyperstimulation protocols with IUI were used in different subgroups in group A.  A total of 216 IUI cycles done for 60 couples. While in group B, 60 males with oligozoospermia were prescribed CC treatment 25mg/day on alternate days for 3 months.Results: Out of 60 patients in group A, 24 achieved pregnancy with a cycle fecundity rate of 4% in CC+IUI, 9.87% in HMG+CC+IUI and 21.67% in HMG+IUI. 5 out of 60 patients in group B achieved pregnancy with cycle fecundity rate of 2.78%.Conclusions: These data suggest that acceptable pregnancy rates can be achieved with IUI combined with ovarian hyperstimulation in males with oligozoospermia. The role of CC treatment in males with oligozoospermia in improving the pregnancy outcome is extremely mearge. Thus, owing to the cost effectiveness and non-invasive modality, IUI is preferred prior to other more invasive and potentially costly reproductive technologies in the treatment of male factor subfertility.

scholarly journals The Effects of Antithrombotic Therapy on Head Trauma and its Management

2021 ◽  
Author(s):  
Takao Koiso ◽  
Yoji Komatsu ◽  
Masayuki Goto ◽  
Toshitsugu Terakado ◽  
Yuji Matsumaru ◽  
...  
Keyword(s):  
Head Trauma ◽  
Poor Prognosis ◽  
Functional Independence ◽  
Optimal Time ◽  
Thromboembolic Events ◽  
Clinical Factors ◽  
Intracranial Hematoma ◽  
Hematoma Enlargement ◽  
Group A ◽  
Group B

Abstract To examine the effects of antithrombotics for head trauma, 393 consecutive patients were enrolled. The patients were divided into those that were (group A, n = 117) and were not (group B, n = 276) taking antithrombotics, and the groups’ outcomes were compared. To identify factors that affected functional independence in group A, clinical factors were compared between the patients that exhibited mRS of 0–2 and 3–6 at discharge. Furthermore, to assess the optimal time to restart antithrombotics, cases in which rebleeding occurred after antithrombotics were restarted or thromboembolic events occurred were extracted. The ratio of mRS 3–6 and death within 30 days were significantly higher in group A than in group B. Multivariate analysis of group A revealed that being aged ≥ 70, not receiving antiplatelet therapy, and intracranial hematoma enlargement were poor prognostic factors. Five patients in group A experienced ischemic strokes within 30 days. In 3 of these patients, the ischemic strokes occurred before antithrombotics were restarted. Rebleeding occurred in two cases after anticoagulants restarted within 48 hours. Patients taking antithrombotics are at high risk of poor prognosis after head trauma. To prevent thromboembolic events, the active resumption of antithrombotics after 48 hours is desirable if hemostasis has been achieved.

scholarly journals Chromatin organization and timing of polar body I extrusion identify developmentally competent mouse oocytes

2019 ◽  
Vol 63 (3-4-5) ◽  
pp. 245-251 ◽  
Author(s):  
Federica Cavalera ◽  
Mario Zanoni ◽  
Valeria Merico ◽  
Lucia Sacchi ◽  
Riccardo Bellazzi ◽  
...  
Keyword(s):  
Dna Binding ◽  
Polar Body ◽  
Time Lapse ◽  
Reproductive Technologies ◽  
Developmental Competence ◽  
Cell Stage ◽  
Germinal Vesicle Breakdown ◽  
Group A ◽  
Group B

In the mouse, the use of the DNA-binding fluorochrome Hoechst 33342 allows the classification of fully-grown antral oocytes into two categories distinguished by their chromatin conformation: surrounding nucleolus (SN) and not-surrounding nucleolus (NSN) oocytes, the former capable of completing development, the latter unable to proceed beyond the 2-cell stage. In the present study, time-lapse observation of SN and NSN oocyte GV-to-MII transition highlighted differences in the timing of germinal vesicle breakdown (GVBD) and polar body I (PB-I) extrusion. PB-I extrusion, but not GVBD, revealed the presence of three main groups of significantly different oocytes: Group A (456-576 min) comprising mainly SN oocytes (91.4%), group B (584-728 min) entailing an almost equivalent percentage of SN (52.7%) and NSN (47.3%) oocytes, whereas group C (736-896 min) consisting of almost all NSN (94.4%) oocytes. In a further set of time-lapse experiments, GV oocytes were in vitro matured without Hoechst staining and, depending on the timing of PB-I extrusion, sorted into group A, B or C, inseminated with sperm and observed throughout preimplantation. The results show that 26.2 &plusmn; 12.3% of group A, 2.4 &plusmn; 5.0% of group B and none of group C MII oocytes developed to blastocyst. Overall, this study shows that SN oocytes that complete MI earlier are those with a better developmental competence. The possibility to avoid the use of the invasive DNA-binding fluorochrome Hoechst is relevant for future applications in human and domestic animal reproductive technologies.

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