scholarly journals SCANNING ELECTRON MICROSCOPY DARI Clinostomum complanatum (DIGENEA: CLINOSTOMIDAE) PADA IKAN BETOK ( Anabas testudineus ) DI YOGYAKARTA, INDONESIA

2011 ◽  
Vol 6 (2) ◽  
pp. 303
Author(s):  
Morina Riauwaty ◽  
Kurniasih Kurniasih ◽  
Joko Prastowo ◽  
Windarti Windarti
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Ventral Sucker ◽  
Oral Sucker ◽  
Anabas Testudineus ◽  
Scanning Electron ◽  
Clinostomum Complanatum

Penelitian ini bertujuan untuk mengetahui topografi permukaan Clinostomum complanatum (Digenea: Clinostomidae) yang menginfeksi ikan air tawar di Yogyakarta. Ikan betok (Anabas testudineus) diperoleh dari Kali Progo, Yogyakarta. Metaserkaria Clinostomum complanatum yang ditemukan di insang, dikeluarkan dengan menggunakan jarum dan diawetkan dalam etanol absolut. Pengamatan topografi permukaan tegument dengan Scanning Electron Microscopy (SEM). Data morfologi dianalisis secara deskriptif. Topografi tegument dari metaserkaria Clinostomum complanatum pada ikan betok menunjukkan adanya perbedaan struktur. Oral sucker terletak di ujung terminal, berbentuk elips dengan permukaan halus. Ventral sucker terletak dekat dengan oral sucker, berada di anterior dan memiliki papila sensoris, tetapi tidak memiliki spina. Permukaan tubuh cekung dan memiliki tonjolan yang tidak beraturan. Lubang ekskretori terletak di ujung posterior tubuh cacing.

scholarly journals Ultrastructural study on morphology of Schistosoma spindale by Scanning electron microscopy (SEM)

2018 ◽  
Vol 10 (1) ◽  
pp. 347-351
Author(s):  
Kommu Sudhakar ◽  
G.S.S. Murthy ◽  
Udaya Kumar. M ◽  
Narasimha Reddy. Y ◽  
Lakshman. M ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Ventral Sucker ◽  
Oral Sucker ◽  
Ultrastructural Study ◽  
Ventral Surface ◽  
Morphological Features ◽  
The Body ◽  
Perfusion Technique ◽  
Scanning Electron

The present study was undertaken to investigate the detailed morphological features along with morphometry of different structures of Schistosoma spindale (Adult flukes) which were recovered by a perfusion technique and visualized by Scanning Electron Microscopy (SEM). The length of spines on the oral sucker and ventral suckers were 2.6 µm and 2.5 µm, respectively. The measured width of aspinose area beneath the ventral sucker, rim of the ventral sucker and tegumental papillae were 5.4, 22.5 µm and 3 µm, respectively. Males have a welldefined gynaecophoric canal, originating just below the ventral sucker and extending up to the posterior end of the body, continued as a marked conical projection. The ventral surface of the oral sucker was completely covered with numerous spines. The ventral sucker was pedunculated, round, thick-rimmed and the inner side contained numerously pointed spines directed towards the center of the ventral sucker. The tegument surface of S. spindale showed ridged layers with large uniciliated and pit like papillae which were recorded more in posterior end. Thus, Scanning Electron microscopy (SEM) provided indepth ultrastructural morphological details of Schistosoma spindale which was in accordance with that of previous studies, would be applicable for its differentiation with other species (S. mansoni, S. bovis, S. haematobium, S. japonicum).

Centrocestus formosanus: surface morphology of metacercaria, adult and egg

1997 ◽  
Vol 71 (4) ◽  
pp. 345-350 ◽  
Author(s):  
T. Srisawangwong ◽  
S. Pinlaor ◽  
P. Kanla ◽  
P. Sithithaworn
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Surface Morphology ◽  
Ventral Sucker ◽  
Oral Sucker ◽  
The Body ◽  
Genital Opening ◽  
Morphological Variations ◽  
Egg Shell ◽  
Scanning Electron

AbstractThe surface morphology of metacercariae isolated from Puntius spp., adult worms from infected hamsters and eggs of Centrocestus formosanus (Digenea: Heterophyidae) were studied using scanning electron microscopy. It was found that the surfaces of the metacercariae and adult worms were closely similar in appearance. The oral sucker was surrounded by a circumoral expansion with two rows of 32 spines (16 spines each). The ventral sucker, with six large nonciliated papillae on the lip, and the genital opening were located midventrally. The excretory pore was terminal. The body surface was covered with pectinate scale-like spines of varying sizes. The scales in the middle area were larger than those in the anterior and posterior parts of the body. Sensory papillae, mostly uniciliated, were present in greater abundance at the anterior region of both stages. However, morphological variations were observed among the adults depending on the age of the worms. At 4 weeks, the oral sucker and circumoral expansion were cobblestone-like in structure and the grooves of spines split compared with their smooth surfaces at 10 days. In addition, the ventral sucker became depressed and its large papillae on the lip disappeared at 10 weeks post infection. Multiciliated papillae were also present in 10-week old worms. Each egg of this parasite possessed a prominent operculum and a latticed design on the egg shell.

Scanning electron microscopy of the collar region of Deropristis inflata and Echinostoma revolutum

1999 ◽  
Vol 73 (1) ◽  
pp. 51-57 ◽  
Author(s):  
I. Kanev ◽  
B.S. Dezfuli ◽  
M. Nestorov ◽  
B. Fried
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Ventral Sucker ◽  
Oral Sucker ◽  
The Body ◽  
General Morphology ◽  
Collar Region ◽  
Echinostoma Revolutum ◽  
Left And Right ◽  
Scanning Electron

Scanning electron microscopy (SEM) was used to study the collar region of Deropristis inflata (Molin, 1858), an intestinal digenean of fish, and Echinostoma revolutum (Froelich, 1802), an intestinal digenean of birds. The results showed basic differences in the following morphological features. The collar of D. inflata was open ventrally and dorsally whereas that of E. revolutum was closed dorsally and ventrally, forming a kidney-like ring. The collar of D. inflata was located posterior to the oral sucker, some distance behind the anterior end of the body. That of E. revolutum was located terminally around the oral sucker. The collar of D. inflata had numerous collar spines which varied in size, shape and position. That of E. revolutum had 37 collar-spines, mainly homogenous in their general morphology and with a typical arrangement pattern for 37-collar-spined echinostomes. The collar of D. inflata had few tegumentary papillae whereas that of E. revolutum had abundant papillae. A ventral depressed area occurred in D. inflata, between the acetabulum and oral sucker, dividing the collar into two left and right independent parts. The ventral depression in E. revolutum extended from the ventral sucker to the posterior end of the collar, and was not divided. In D. inflata, tegumentary spines were located on the oral sucker, and the collar was posterior to the sucker. In E. revolutum, the oral sucker and the collar lacked tegumentary spines. The region immediately posterior to the collar also lacked spines in E. revolutum. The collar pattern of D. inflata is undoubtedly more primitive than that of E. revolutum.

Scanning electron microscopy of the integument of schistosoma rodhaini

1986 ◽  
Vol 44 ◽  
pp. 132-133
Author(s):  
P. Evers ◽  
C. Schutte ◽  
C. D. Dettman
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Oral Sucker ◽  
Adult Male ◽  
Dorsal Surface ◽  
Ventral Surface ◽  
Sensory Receptors ◽  
East African ◽  
Dorsal Midline ◽  
Scanning Electron

S.rodhaini (Brumpt 1931) is a parasite of East African rodents which may possibly hybridize with the human schistosome S. mansoni. The adult male at maturity measures approximately 3mm long and possesses both oral and ventral suckers and a marked gynaecophoric canal. The oral sucker is surrounded by a ring of sensory receptors with a large number of inwardly-pointing spines set into deep sockets occupying the bulk of the ventral surface of the sucker. Numbers of scattered sensory receptors are found on both dorsal and ventral surfaces of the head (Fig. 1) together with two conspicuous rows of receptors situated symmetrically on each side of the midline. One row extends along the dorsal surface of the head midway between the dorsal midline and the lateral margin.

A new freshwater leech species of Helobdella (Annelida: Glossiphoniidae) from central Mexico

Zootaxa ◽  
2005 ◽  
Vol 976 (1) ◽  
pp. 1 ◽  
Author(s):  
ALEJANDRO OCEGUERA-FIGUEROA ◽  
VIRGINIA LEON-REGAGNON
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Oral Sucker ◽  
Anterior Margin ◽  
Central Mexico ◽  
Diagnostic Characters ◽  
Posterior Extension ◽  
Scanning Electron

Helobdella atli n sp. is described based on the examination of 20 specimens collected in Totolcingo Lake, Tlaxcala, Mexico. Leeches were found attached to submerged rocks and plants. Study of specimens included optical and scanning electron microscopy. Diagnostic characters are: sperm ducts with a long posterior extension, a dorsal nuchal scute on somite VIII, postcaeca absent in the last pair of crop caeca and the mouth pore in the anterior margin of the oral sucker. Previous records of H. adiastola in Mexico correspond to H. atli n. sp.

Observations on the musculature and isolated muscle fibres of the liver fluke,Fasciola hepatica

Parasitology ◽  
2003 ◽  
Vol 127 (5) ◽  
pp. 457-473 ◽  
Author(s):  
D. KUMAR ◽  
J. G. McGEOWN ◽  
O. REYNOSO-DUCOING ◽  
J. R. AMBROSIO ◽  
I. FAIRWEATHER
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Ventral Sucker ◽  
Liver Fluke ◽  
Fasciola Hepatica ◽  
Muscle Fibres ◽  
Muscle Proteins ◽  
Somatic Muscle ◽  
Phalloidin Staining ◽  
Scanning Electron

The liver fluke,Fasciola hepaticarelies on a well-developed muscular system, not only for attachment, but for many aspects of its biology. Despite this, little is known about the system beyond the gross organization of the main somatic muscle layers. In the present study, a range of techniques have been applied toF. hepaticain order to understand more about various aspects of muscle organization, biochemistry (in terms of muscle proteins) and identity of isolated muscle fibres. Scanning electron microscopy has provided a direct visualizationin situof the somatic muscle layers and the organization of the muscle fibres within the ventral sucker. The muscle bundles contributing to the main somatic muscle layers are made up of up to 10 individual muscle fibres. Phalloidin staining for actin, in conjunction with confocal microscopy, confirmed the presence of 2 main somatic muscle layers (outer circular, inner longitudinal), beneath which lies a third layer of oblique muscle fibres. The use of propidium iodide in combination with phalloidin staining for actin demonstrated that the cell bodies associated with the 2 main somatic muscle layers are situated beneath the longitudinal muscle layer and are connected to their respective muscle fibres by short cytoplasmic processes. Myosin immunoreactivity was demonstrated in the somatic muscle layers and in the muscle layers surrounding various organ systems within the fluke. Double labelling for actin and myosin confirmed the co-localization of the 2 muscle proteins in the muscle fibres of the ventral sucker. Muscle fibres from the somatic muscle layers and the ventral sucker have been isolated and images obtained with phase-contrast microscopy and scanning electron microscopy. The muscle fibres contain actin and myosin, but lack a nucleus, the connection with the cell body having been broken during the isolation procedure.

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

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