Scanning electron microscopy of the collar region of Deropristis inflata and Echinostoma revolutum

1999 ◽  
Vol 73 (1) ◽  
pp. 51-57 ◽  
Author(s):  
I. Kanev ◽  
B.S. Dezfuli ◽  
M. Nestorov ◽  
B. Fried

Scanning electron microscopy (SEM) was used to study the collar region of Deropristis inflata (Molin, 1858), an intestinal digenean of fish, and Echinostoma revolutum (Froelich, 1802), an intestinal digenean of birds. The results showed basic differences in the following morphological features. The collar of D. inflata was open ventrally and dorsally whereas that of E. revolutum was closed dorsally and ventrally, forming a kidney-like ring. The collar of D. inflata was located posterior to the oral sucker, some distance behind the anterior end of the body. That of E. revolutum was located terminally around the oral sucker. The collar of D. inflata had numerous collar spines which varied in size, shape and position. That of E. revolutum had 37 collar-spines, mainly homogenous in their general morphology and with a typical arrangement pattern for 37-collar-spined echinostomes. The collar of D. inflata had few tegumentary papillae whereas that of E. revolutum had abundant papillae. A ventral depressed area occurred in D. inflata, between the acetabulum and oral sucker, dividing the collar into two left and right independent parts. The ventral depression in E. revolutum extended from the ventral sucker to the posterior end of the collar, and was not divided. In D. inflata, tegumentary spines were located on the oral sucker, and the collar was posterior to the sucker. In E. revolutum, the oral sucker and the collar lacked tegumentary spines. The region immediately posterior to the collar also lacked spines in E. revolutum. The collar pattern of D. inflata is undoubtedly more primitive than that of E. revolutum.

2018 ◽  
Vol 10 (1) ◽  
pp. 347-351
Author(s):  
Kommu Sudhakar ◽  
G.S.S. Murthy ◽  
Udaya Kumar. M ◽  
Narasimha Reddy. Y ◽  
Lakshman. M ◽  
...  

The present study was undertaken to investigate the detailed morphological features along with morphometry of different structures of Schistosoma spindale (Adult flukes) which were recovered by a perfusion technique and visualized by Scanning Electron Microscopy (SEM). The length of spines on the oral sucker and ventral suckers were 2.6 µm and 2.5 µm, respectively. The measured width of aspinose area beneath the ventral sucker, rim of the ventral sucker and tegumental papillae were 5.4, 22.5 µm and 3 µm, respectively. Males have a welldefined gynaecophoric canal, originating just below the ventral sucker and extending up to the posterior end of the body, continued as a marked conical projection. The ventral surface of the oral sucker was completely covered with numerous spines. The ventral sucker was pedunculated, round, thick-rimmed and the inner side contained numerously pointed spines directed towards the center of the ventral sucker. The tegument surface of S. spindale showed ridged layers with large uniciliated and pit like papillae which were recorded more in posterior end. Thus, Scanning Electron microscopy (SEM) provided indepth ultrastructural morphological details of Schistosoma spindale which was in accordance with that of previous studies, would be applicable for its differentiation with other species (S. mansoni, S. bovis, S. haematobium, S. japonicum).


1997 ◽  
Vol 71 (4) ◽  
pp. 345-350 ◽  
Author(s):  
T. Srisawangwong ◽  
S. Pinlaor ◽  
P. Kanla ◽  
P. Sithithaworn

AbstractThe surface morphology of metacercariae isolated from Puntius spp., adult worms from infected hamsters and eggs of Centrocestus formosanus (Digenea: Heterophyidae) were studied using scanning electron microscopy. It was found that the surfaces of the metacercariae and adult worms were closely similar in appearance. The oral sucker was surrounded by a circumoral expansion with two rows of 32 spines (16 spines each). The ventral sucker, with six large nonciliated papillae on the lip, and the genital opening were located midventrally. The excretory pore was terminal. The body surface was covered with pectinate scale-like spines of varying sizes. The scales in the middle area were larger than those in the anterior and posterior parts of the body. Sensory papillae, mostly uniciliated, were present in greater abundance at the anterior region of both stages. However, morphological variations were observed among the adults depending on the age of the worms. At 4 weeks, the oral sucker and circumoral expansion were cobblestone-like in structure and the grooves of spines split compared with their smooth surfaces at 10 days. In addition, the ventral sucker became depressed and its large papillae on the lip disappeared at 10 weeks post infection. Multiciliated papillae were also present in 10-week old worms. Each egg of this parasite possessed a prominent operculum and a latticed design on the egg shell.


2011 ◽  
Vol 6 (2) ◽  
pp. 303
Author(s):  
Morina Riauwaty ◽  
Kurniasih Kurniasih ◽  
Joko Prastowo ◽  
Windarti Windarti

Penelitian ini bertujuan untuk mengetahui topografi permukaan Clinostomum complanatum (Digenea: Clinostomidae) yang menginfeksi ikan air tawar di Yogyakarta. Ikan betok (Anabas testudineus) diperoleh dari Kali Progo, Yogyakarta. Metaserkaria Clinostomum complanatum yang ditemukan di insang, dikeluarkan dengan menggunakan jarum dan diawetkan dalam etanol absolut. Pengamatan topografi permukaan tegument dengan Scanning Electron Microscopy (SEM). Data morfologi dianalisis secara deskriptif. Topografi tegument dari metaserkaria Clinostomum complanatum pada ikan betok menunjukkan adanya perbedaan struktur. Oral sucker terletak di ujung terminal, berbentuk elips dengan permukaan halus. Ventral sucker terletak dekat dengan oral sucker, berada di anterior dan memiliki papila sensoris, tetapi tidak memiliki spina. Permukaan tubuh cekung dan memiliki tonjolan yang tidak beraturan. Lubang ekskretori terletak di ujung posterior tubuh cacing.


Author(s):  
P. Evers ◽  
C. Schutte ◽  
C. D. Dettman

S.rodhaini (Brumpt 1931) is a parasite of East African rodents which may possibly hybridize with the human schistosome S. mansoni. The adult male at maturity measures approximately 3mm long and possesses both oral and ventral suckers and a marked gynaecophoric canal. The oral sucker is surrounded by a ring of sensory receptors with a large number of inwardly-pointing spines set into deep sockets occupying the bulk of the ventral surface of the sucker. Numbers of scattered sensory receptors are found on both dorsal and ventral surfaces of the head (Fig. 1) together with two conspicuous rows of receptors situated symmetrically on each side of the midline. One row extends along the dorsal surface of the head midway between the dorsal midline and the lateral margin.


1972 ◽  
Vol 3 (3) ◽  
pp. 181-188 ◽  
Author(s):  
Christine Dahl

AbstractA method for preparation of alcohol-preserved culicid larvae for Scanning Electron Microscope (SEM) studies is described. It is based on dehydration by ethanol-xylol and fast evaporation of xylol in +8o° C. for ten minutes. For taxonomic studies such as examination of pecten teeth, comb scales and microtrichiae in magnifications up to 6oooX the method is suitable. For studies of receptor structures on hair-tufts and microstructures of the body integument alcohol preserved material is less satisfactory. The microstructure of the comb scales is figured and their function discussed. Differences in the ultrastructure of the abdominal hair-tufts are pointed out.


2010 ◽  
Vol 55 (No. 6) ◽  
pp. 275-280 ◽  
Author(s):  
NS Kim ◽  
HC Kim ◽  
C. Sim ◽  
JR Ji ◽  
NS Kim ◽  
...  

In March 2010, a 3.5-month-old Korean native calf was anatomized and two nematode worms were detected in the thoracic cavity. The worms were identified and classified by light and scanning electron microscopy on the basis of features at the anterior and posterior parts of the worms. The worms were female Setaria digitata and Setaria marshalli and numerous eggs which contained microfilaria were detected in the uterus of both species. The body lengths of the S. digitata and S. marshalli were 78 mm and 117 mm, respectively. Mosquitoes act as the vector for Setaria nematodes but these are inactivated in winter in Korea. Therefore, we concluded that this case represented setariasis with congenital infection occurring during the summer prenatal stage of life.


Parasite ◽  
2018 ◽  
Vol 25 ◽  
pp. 29 ◽  
Author(s):  
Weishan Zhao ◽  
Can Li ◽  
Dong Zhang ◽  
Runqiu Wang ◽  
Yingzhen Zheng ◽  
...  

Balantidium grimi n. sp. is described from the rectum of the frog Quasipaa spinosa (Amphibia, Dicroglossidae) from Lishui, Zhejiang Province, China. The new species is described by both light microscopy (LM) and scanning electron microscopy (SEM), and a molecular phylogenetic analysis is also presented. This species has unique morphological features in that the body shape is somewhat flattened and the vestibulum is “V”-shaped, occupying nearly 3/8 to 4/7 of the body length. Only one contractile vacuole, situated at the posterior body, was observed. The phylogenetic analysis based on SSU-rDNA indicates that B. grimi groups together with B. duodeni and B. entozoon. In addition, the genus Balantidium is clearly polyphyletic.


Author(s):  
J.F. Putterill ◽  
J.T. Soley

The heads of nine 2.5 to 3-year-old Nile crocodiles (Crocodylus niloticus) were obtained from a commercial farm where crocodiles are raised for their skins and meat. The animals from which these specimens originated were clinically healthy at the time they were slaughtered. A detailed description of the macroscopic and microscopic features of the palate and gingivae of the Nile crocodile is presented and the results are compared with published information on this species and other Crocodylia. The histological features are supplemented by information supplied by scanning electron microscopy. Macroscopic features of interest are the small conical process situated at the base of the first two incisors of the maxilla, the distribution of cobbled units on the palate, and the broad dentary shelf forming the rostral aspect of the mandible. Histologically the palate and gingivae did not differ significantly from each other and both regions showed a presence of Pacinian-type corpuscles. Two types of sensory structures (taste receptors and pressure receptors) were identified in the regions examined, both involving modification of the epithelium and the underlying connective tissue.


2011 ◽  
Vol 295-297 ◽  
pp. 869-872
Author(s):  
Qing Shan Li ◽  
Xin Wang ◽  
Jun Liu ◽  
Guang Zhong Xing

Six-ring Rock is widely used as containers of water and additives to produce health care products. In this paper, the composition and microstructure of Six-ring Rock have been investigated by using scanning electron microscopy, energy dispersive spectrometer, transmission electron microscopy, x-ray diffraction and other technologies. Results show that Six-ring Rock is composed of CaMg(CO3)2, SiO2 and KAlSi3O8. Fe atoms exist in CaMg(CO3)2 by replacing Mg atoms. Six-ring Rock shows nano-size lamellar and acerose microstructures on the surface, and nano-size monocrystals in the body. Six-ring Rock is a natural nano structure mineral.


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