The Application of a Compact Multispectral Imaging System with Integrated Excitation Source to In vivo Monitoring of Fluorescence During Topical Photodynamic Therapy of Superficial Skin Cancers¶

In this study, a laparoscopic imaging device and a light source able to select wavelengths by bandpass filters were developed to perform multispectral imaging (MSI) using over 1000 nm near-infrared (OTN-NIR) on regions under a laparoscope. Subsequently, MSI (wavelengths: 1000–1400 nm) was performed using the built device on nine live mice before and after tumor implantation. The normal and tumor pixels captured within the mice were used as teaching data sets, and the tumor-implanted mice data were classified using a neural network applied following a leave-one-out cross-validation procedure. The system provided a specificity of 89.5%, a sensitivity of 53.5%, and an accuracy of 87.8% for subcutaneous tumor discrimination. Aggregated true-positive (TP) pixels were confirmed in all tumor-implanted mice, which indicated that the laparoscopic OTN-NIR MSI could potentially be applied in vivo for classifying target lesions such as cancer in deep tissues.

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TISSUE OXYGEN LEVEL IN ACUTE AND CHRONIC WOUND: A COMPARISON STUDY

Jurnal Teknologi ◽

10.11113/jt.v82.14418 ◽

2020 ◽

Vol 82 (4) ◽

Author(s):

Sheena P. Philimon ◽

Audrey K. C. Huong ◽

Xavier T. I. Ngu

Keyword(s):

Multispectral Imaging ◽

Chronic Wounds ◽

Imaging System ◽

Clinical Information ◽

Transcutaneous Oxygen ◽

In Vivo Measurement ◽

Randomized Clinical Study ◽

Transcutaneous Oxygen Saturation ◽

The Difference

Adequate oxygen supply to granulating tissue and its surroundings is considered as an elementary factor to render an optimum condition for proper wound healing. This paper focused on comparing the quantitative mean of transcutaneous oxygen saturation (StO2) between acute superficial wounds and chronic diabetic wounds in a randomized clinical study. A multispectral imaging system was employed for in-vivo measurement of wound StO2 across a visible wavelength range of nm. Processed StO2 maps of wounds using a reconstructed mathematical model revealed a mean StO2 of and , respectively, in acute and chronic wounds. The statistical test on the difference in the values between these two groups of wounds was shown to be statistically significant (). These crucial findings contribute to the existing knowledge on the StO2 range and its variation in different wound conditions. In conclusion, the proposed multispectral imaging system is able to produce clinical information of wound oxygenation level for a better understanding of the obligatory role of oxygen as a key determinant in healing outcomes.

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Improved in vivo Fluorescence Tomography and Quantitation in Small Animals Using a Novel Multiview, Multispectral Imaging System

Biomedical Optics and 3-D Imaging ◽

10.1364/biomed.2010.btuf1 ◽

2010 ◽

Author(s):

Craig Gardner ◽

Joyita Dutta ◽

Gregory S. Mitchell ◽

Sangtae Ahn ◽

Changqing Li ◽

...

Keyword(s):

Multispectral Imaging ◽

Imaging System ◽

Small Animals ◽

Fluorescence Tomography ◽

In Vivo Fluorescence

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In vivo monitoring of Foscan-mediated photodynamic therapy in clinical head and neck procedures using optical spectroscopy

Head & Neck Oncology ◽

10.1186/1758-3284-2-s1-o16 ◽

2010 ◽

Vol 2 (S1) ◽

Author(s):

Stephen Kanick ◽

Baris Karakullukcu ◽

Robert van Veen ◽

Henricus Sterenborg ◽

I Bing Tan ◽

...

Keyword(s):

Photodynamic Therapy ◽

Head And Neck ◽

Optical Spectroscopy ◽

In Vivo Monitoring

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Daylight Photodynamic Therapy: An Update

Molecules ◽

10.3390/molecules25215195 ◽

2020 ◽

Vol 25 (21) ◽

pp. 5195

Author(s):

Chaw-Ning Lee ◽

Rosie Hsu ◽

Hsuan Chen ◽

Tak-Wah Wong

Keyword(s):

Photodynamic Therapy ◽

Skin Cancer ◽

20Th Century ◽

Actinic Keratosis ◽

Skin Diseases ◽

Aminolevulinic Acid ◽

Review Article ◽

Skin Cancers ◽

Superficial Skin ◽

Pros And Cons

Daylight photodynamic therapy (dPDT) uses sunlight as a light source to treat superficial skin cancer. Using sunlight as a therapeutic device has been present for centuries, forming the basis of photodynamic therapy in the 20th century. Compared to conventional PDT, dPDT can be a less painful, more convenient and an effective alternative. The first clinical uses of dPDT on skin cancers began in Copenhagen in 2008. Currently, aminolevulinic acid-mediated dPDT has been approved to treat actinic keratosis patients in Europe. In this review article, we introduce the history and mechanism of dPDT and focus on the pros and cons of dPDT in treating superficial skin cancers. The future applications of dPDT on other skin diseases are expected to expand as conventional PDT evolves.

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In vivo monitoring of photosensitizer fluorescence during photodynamic therapy

10.1117/12.203433 ◽

1994 ◽

Cited By ~ 8

Author(s):

Mark R. Stringer ◽

Dominic J. Robinson ◽

Emma J. Hudson ◽

Michael A. Smith

Keyword(s):

Photodynamic Therapy ◽

In Vivo Monitoring

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Targeted Photodynamic Therapy (PDT) of Lung Cancer with Biotinylated Silicon (IV) Phthalocyanine.

Current Pharmaceutical Biotechnology ◽

10.2174/1389201021666200510001627 ◽

2020 ◽

Vol 21 ◽

Author(s):

Wenyi Dong ◽

Ke Li ◽

Shijie Wang ◽

Ling Qiu ◽

Qingzhu Liu ◽

...

Keyword(s):

Lung Cancer ◽

Photodynamic Therapy ◽

Imaging System ◽

A549 Cells ◽

Human Lung Cancer ◽

In Vitro Assays ◽

Cancer Therapies ◽

Lung Cancers

Background: Lung cancer is the leading cause of cancer-associated mortality in the world. Traditional cancer therapies prolong life expectancy of patients but often suffer from adverse reactions. Photodynamic therapy (PDT) has been recommended as a treatment option for lung cancer in several countries, due to its non-invasive procedures, high selectivity and weak side effects. Objective: We have designed and synthesized a biotin receptor-targeted silicon phthalocyanine (IV) (compound 1) which showed good therapeutic effect on biotin receptor-positive tumors. Since the overexpression of biotin receptor (BR) is also present in human lung cancer cells (A549), we explored the therapeutic properties of compound 1 on A549 xenograft tumor models. Method: The selectivity of compound 1 toward A549 cells was studied with fluorescence microscope and IVIS Spectrum Imaging System. The cytotoxicity was measured using MTT assay. In vivo anti-tumor activity was investigated on the nude mice bearing A549 xenografts. Results: In vitro assays proved that compound 1 could selectively accumulate in A549 cells via the BR-mediated internalization. In vivo imaging and distribution experiments showed that compound 1 could selectively accumulate in tumor tissues of tumor-bearing mice. After 16 days of the treatment, the volumes of tumor in PDT group were obviously smaller than that in other groups. Conclusion: This study demonstrates that compound 1 is a promising photosensitizer and has broad application prospects in clinical PDT of lung cancers.

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Functional Imaging of the Ocular Fundus Using an 8-Band Retinal Multispectral Imaging System

Instruments ◽

10.3390/instruments4020012 ◽

2020 ◽

Vol 4 (2) ◽

pp. 12

Author(s):

Emanuel R. de Carvalho ◽

Richelle J. M. Hoveling ◽

Cornelis J. F. van Noorden ◽

Reinier O. Schlingemann ◽

Maurice C. G. Aalders

Keyword(s):

Oxygen Saturation ◽

Functional Imaging ◽

Multispectral Imaging ◽

Imaging System ◽

Imaging Techniques ◽

Incident Light ◽

Contrast Imaging ◽

Blood Oxygen Saturation ◽

Full Scan

Application of functional imaging in ophthalmology requires efficient imaging techniques that can detect and quantify chromophores to visualise processes in vivo. The aim of the present study was to develop and evaluate a fast and affordable imaging system. We describe an eight-band retinal multispectral imaging (MSI) system and compare it with a hyperspectral imaging (HSI) device. Determination of blood oxygen saturation was studied as proof of principle. Reflectance of incident light is measured as 1/absorbance at different wavelengths between 440 nm and 580 nm. Both devices have incorporated optical bandpass filters in a mydriatic fundus camera. The MSI system scans the retina at eight pre-defined wavelengths specific for the spectrum of haemoglobin. The HSI system acquires a full scan from 480 to 720 nm in 5 nm steps. A simple assessment of the ratio between the absorbance peaks of oxygenated haemoglobin (HbO2) and reduced haemoglobin (HbR) was not suitable for generating validated oxygenation maps of the retina. However, a correction algorithm that compares the measured reflectance with reflectance spectra of fully oxygenated and fully deoxygenated blood allowed our MSI setup to estimate relative oxygen saturation at higher levels, but underestimated relative oxygen saturation at lower levels. The MSI device generated better quality images than the HSI device. It allows customisation with filter sets optimised for other chromophores of interest, and augmented with extrinsic contrast imaging agents, it has the potential for a wider range of ophthalmic molecular imaging applications.

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