SCREENING OF PEPTIDE RECEPTOR SEQUENCES FOR AMITROL DETECTION USING CHROMATOGRAPHIC BIOPANNING

Endocrine disrupting chemicals (EDCs) have been known as health threatening compounds and much attention has been paid in a research related with detection and/or removal from food sources and living environment. Amitrol, classified as one of EDCs, is hard to be detected and removed due to its chemical characteristics such as small molecular weight, low reactivity and high solubility. Some of organic compounds were investigated to find the corresponding binding peptides to those by biopanning protocol. However, peptides with affinity to amitrol remained to be searched for because amitrol is hard to be used as a target of free form or immobilized form in common biopanning protocol. In our experiment, amitrol was successfully immobilized on CDI monolithic column with the presence of catalyst and hence was able to be applied as a target component in biopanning. Chromatic biopanning was carried out as a fast and convenient method for the selection of peptides with high affinity to amitrol. Chemical and physical methods were combined in elution step to improve the selection efficiency for strong binders. After multiple rounds of negative screening and positive screening, high affinity peptide sequences were isolated from initial peptide libraries. Applications of these peptide sequences can be widened from bio adsorption to biosensor for environment pollution control.

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Effect of Polyphosphate on Removal of Endocrine-Disrupting Chemicals of Nonylphenol and Bisphenol-A by Activated Carbons

Water Quality Research Journal ◽

10.2166/wqrj.2005.052 ◽

2005 ◽

Vol 40 (4) ◽

pp. 484-490 ◽

Cited By ~ 5

Author(s):

Keun J. Choi ◽

Sang G. Kim ◽

Chang W. Kim ◽

Seung H. Kim

Keyword(s):

Activated Carbon ◽

Bisphenol A ◽

Surface Charge ◽

Activated Carbons ◽

Endocrine Disrupting Chemicals ◽

Dipole Interaction ◽

High Affinity ◽

Calcium Polyphosphate ◽

Endocrine Disrupting ◽

Positively Charged

Abstract This study examined the effect of polyphosphate on removal of endocrine-disrupting chemicals (EDCs) such as nonylphenol and bisphenol-A by activated carbons. It was found that polyphosphate aided in the removal of nonylphenol and bisphenol- A. Polyphosphate reacted with nonylphenol, likely through dipole-dipole interaction, which then improved the nonylphenol removal. Calcium interfered with this reaction by causing competition. It was found that polyphosphate could accumulate on carbon while treating a river. The accumulated polyphosphate then aided nonylphenol removal. The extent of accumulation was dependent on the type of carbon. The accumulation occurred more extensively with the wood-based used carbon than with the coal-based used carbon due to the surface charge of the carbon. The negatively charged wood-based carbon attracted the positively charged calcium-polyphosphate complex more strongly than the uncharged coal-based carbon. The polyphosphate-coated activated carbon was also effective in nonylphenol removal. The effect was different depending on the type of carbon. Polyphosphate readily attached onto the wood-based carbon due to its high affinity for polyphosphate. The attached polyphosphate then improved the nonylphenol removal. However, the coating failed to attach polyphosphate onto the coal-based carbon. The nonylphenol removal performance of the coal-based carbon remained unchanged after the polyphosphate coating.

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Sorption and toxicity reduction of pharmaceutically active compounds and endocrine disrupting chemicals in the presence of colloidal humic acid

Water Science & Technology ◽

10.2166/wst.2016.270 ◽

2016 ◽

Vol 74 (4) ◽

pp. 904-913 ◽

Cited By ~ 7

Author(s):

Injeong Kim ◽

Hyo-Dong Kim ◽

Tae-Yong Jeong ◽

Sang Don Kim

Keyword(s):

Humic Acid ◽

Growth Inhibition ◽

Removal Efficiency ◽

Endocrine Disrupting Chemicals ◽

Algal Growth ◽

Free Form ◽

Natural Environments ◽

Pharmaceutically Active Compounds ◽

Active Compounds ◽

Endocrine Disrupting

This study investigated the toxicity changes and sorption of pharmaceuticals and endocrine disrupters in the presence of humic acid (HA). For the sorption experiment, a dead end filtration (DEF) system was used to separate bound and free-form target compounds. An algae growth inhibition test and E-screen assay were conducted to estimate the toxic effect of pharmaceutically active compounds (PhACs) and endocrine disrupting chemicals (EDCs), respectively. The permeate concentration was confirmed using liquid chromatography–mass spectrometry. In the sorption test, we observed significant sorption of PhACs and EDCs on colloidal HA, except for sulfamethoxazole (SMX). The values of log KCOC derived from DEF determinations ranged from 4.40 to 5.03. The removal efficiency varied with the HA concentration and the target chemical properties. Tetracycline and 4-octylphenol showed the highest sorption or removal efficiency (≈50%), even at 5 mg C/L HA. The algal growth inhibition of PhACs and the estrogenic effects of EDCs were significantly decreased in proportion to HA concentrations, except for SMX. In addition, the chemical analysis results showed a positive relationship with the bioassay results. Consequently, the sorption of PhACs and EDCs onto colloidal HA should be emphasized in natural environments because it significantly reduces bioavailable concentrations and toxicity to aquatic organisms.

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