scholarly journals ANTIBACTERIAL ACTIVITIES OF THE ESSENTIAL OIL OF VIETNAMESE HOLY BASIL (Ocimum sanctum L.) AGAINST FOOD-BORNE BACTERIA AND FUNGI SPECIES

2018 ◽  
Vol 56 (4A) ◽  
pp. 205
Author(s):  
THI TO QUYNH CUNG
Keyword(s):  
Candida Albicans ◽  
Essential Oil ◽  
Rhizoctonia Solani ◽  
Fusarium Oxysporum ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Ocimum Sanctum ◽  
Food Borne ◽  
Bacteria And Fungi ◽  
Holy Basil

Antimicrobial activities of essential oil (EO) of Vietnamese holy basil (Ocimum sanctum L.) were screened against several food-borne bacteria and fungi species. The main volatile components detected in the holy basil EO were eugenol (53.61%), (E)-caryophyllene (25.37%), (β)-elemene (15.86%), germacrene A (2.03%). A significant growth inhibition of this EO with some different concentrations against Escherichia coli ATCC® 25922™ (E. coli), Staphylococcus aureus ATCC ® 25923 ™ (S. aureus), Bacillus subtilis ATCC® 11774™ (B.subtilis), Bacillus cereus ATCC® 13061™ (B.cereus) and Candida albicans ATCC 10231, Rhizoctonia solani, Fusarium oxysporum were observed by the standard disc diffusion method. The inhibition zone for each isolate by holy basil EO obtained from 5 µl and 10 µl of EO ranged from 15.3 - 16.4 mm and 16.7 – 18.4 mm in diameter, respectively. The minimum inhibitory concentration (MIC) of holy basil EO was 0.78 µl/ml determined by a micro-dilution method in MHB liquid medium. The bactericidal concentrations (MBC) was 1.56 µl/ml in all isolates tested. The antifungal properties of the holy basil EO were also determined in this study against three important pathogenic fungi such as Candida albicans, Rhizoctonia solani and Fusarium oxysporum with the inhibition zone ranges from 20 to 30 mm. The results suggested that holy basil EO can be applied in food industries as natural flavoring preservatives/additives to control food spoilage and food-borne pathogenic bacteria and fungi causing severe destruction in food.

scholarly journals VOLATILE COMPOSITION, ANTIOXIDANT PROPERTY AND ANTIMICROBIAL ACTIVITIES AGAINST FOOD-BORNE BACTERIA OF VIETNAMESE THYME (Thymus vulgaris L.) ESSENTIAL OIL

2019 ◽  
Vol 57 (3B) ◽  
pp. 127 ◽  
Author(s):  
Cung Thi To Quynh ◽  
Vu Thu Trang
Keyword(s):  
Essential Oil ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
Dilution Method ◽  
Volatile Components ◽  
E Coli ◽  
Food Borne ◽  
Inhibition Zones ◽  
Bacteria And Fungi

The essential oil (EO) obtained from the leaves of the thyme (Thymus vulgaris L.) grown in Vietnam was found to contain thymol (39.79%), cymene (17.33%), and γ-terpinene (13.45%) as the main volatile components. The antimicrobial activities of this oil were screened against several food-borne bacteria and fungi species. Significant growth inhibition effects against food-borne bacteria Escherichia coli, Staphylococcus aureus, Bacillus cereus, and Salmonella Typhimurium were observed using the standard disc diffusion method. Thyme EO showed the antibacterial effect against all the test pathogenic strains with the inhibition zones were 23.3 ± 0.4 mm, 24.7 ± 0.4 mm, 29.0 ± 0.7 mm, 32 ± 0.7 mm in diameter against B. subtilis,  E. coli, S. Typhimurium and S. aureus, respectively. The minimum inhibitory concentration (MIC) determined by micro-dilution method in MHB liquid medium was 1.56 µl/ml. The bactericidal concentrations (MBC) was 3.13 µl/ml for three isolates from B. subtilis,  E. coli, and S. aureus, while the MBC tested for S. typhi was 1.56 µl/ml. The antifungal properties of the thyme EO were also determined in this study against three important pathogenic fungi such as Candida albicans, Rhizoctonia solani and Fusarium oxysporum with the inhibition zones ranging approximately from 23.20 ± 0.06 to 44.10 ± 0.03 mm. On the other hands, the results also showed the antioxidant activity of Vietnamese thyme EO and suggested that thyme EO can be applied in food industries as natural flavoring preservatives/additives to control food spoilage and food born bacteria and fungi.

scholarly journals Evaluation of Anti Microbial and Anti Fungal Activity of Acalyphaindica L., Leaf Extract

2018 ◽  
Vol 10 (01) ◽  
Author(s):  
Sudhakar Chekuri ◽  
Arun Jyoti B ◽  
Saraswathi JSompaga ◽  
Shivaprasadi Panjala ◽  
Roja Ran Anupalli
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Fungal Activity ◽  
Bacteria And Fungi ◽  
Anti Fungal ◽  
Acalypha Indica

Anti-microbial and anti-fungal activity of different solvent extracts of Acalypha indica (Euphorbeace family) was tested against bacterial pathogens (Pseudomonasaeruginosa, E.Coli, KlebsiellaPneumonia and Staphylococcus aureus) and fungal strains (Candida albicans, Aspergillus niger, Candida tropicalis andCandida kefyr) using the Agar Well diffusion method . It was observed that all the extracts showed positive activity) against bacteria and fungi. Ethanolic extract of Acalypha indica showed more potency against Staphylococcus aureus with an inhibition zone of12.46 (mm) and Methanolic extract exhibited higher activity against E.coli with an inhibition zone of11.26 (mm). Ethanolic extract of Acalypha indica showed prominent antifungal activity against candida albicans with an inhibition diameter of 12.53 (mm) and Aspergillus niger with a diameter of 9.21 (mm) when compared to other solvent extracts. Erythromycin and Ketoconazole were used as positive standards for antimicrobial and anti fungal experiments. In the present study, Ethanol extract showed a varying degree of inhibition to the growth of tested organisms compared to Methanol, Acetone and Chloroform against Bacteria and Fungi. The results confirmed the presence of antibacterial and antifungal compounds in shade dried extracts of Acalypha indica against human pathogenic organisms.

scholarly journals Daya Hambat Minyak Atsiri Rimpang Kunyit terhadap Pertumbuhan Candida Albicans In Vitro

2018 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Fitri Nadifah ◽  
Nurlaili Farida Muhajir ◽  
Fitri Retnoningsih
Keyword(s):  
Candida Albicans ◽  
Essential Oil ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Inhibition Activity ◽  
Significant Difference ◽  
Oil Concentration ◽  
Anti Fungal ◽  
One Way Anova

Abstract: Inhibition Activity Of Turmeric Essential Oil Against The Growth Of Candida Albicans. Turmeric has rhizomes which contain essential oils and curcuminoid that has anti-fungal activity. The purpose of this research was to know the influence of essential oil of turmeric Rhizome (Curcuma domestica Val.) against the growth of Candida albicans in vitro. This was an experimental research with diffusion method. Essential oil obtained through distillation methods and the concentration used was 20%, 40%, 60%, 80% and 100%. The treatment was repeated three times. Anova test was used. Inhibition zone is formed at a concentration of 20%, 40%, 60%, 80%, 100% were 6,16 mm, 6,50 mm, 6,66 mm, 7,00 mm and 7.83 mm, respectively Results in analysis by one way ANOVA showed there was a significant difference in the variance of essential oil concentration against the inhibition zone (p< 0.05).Abstrak: Daya Hambat Minyak Atsiri Rimpang Kunyit Terhadap Pertumbuhan Candida Albicans In Vitro. Kunyit merupakan  salah satu rimpang yang diketahui memiliki kandungan minyak atsiri serta curcuminoid yang bersifat anti jamur. Tujuan penelitian ini untuk mengetahui pengaruh minyak atsiri rimpang kunyit (Curcuma domestica Val.) terhadap pertumbuhan Candida albicans secara in vitro. Jenis penelitian ini adalah penelitian eksperimental dengan metode difusi cara sumuran. Minyak atsiri didapatkan melalui   metode destilasi dan konsentrasi  yang digunakan adalah 20%, 40%, 60%, 80% dan 100%. Perlakuan diulang sebanyak tiga kali. Hasil penelitian di analisis dengan Anova. Hasil zona hambat yang terbentuk pada konsentrasi minyak atsiri 20%, 40%, 60%, 80%, 100% berturut-turut adalah 6,16 mm, 6,50 mm, 6,66 mm, 7,00 mm,  dan 7,83 mm. Hasil analisis dengan one way anova menunjukkan terdapat perbedaan yang signfikan pada berbagai konsentrasi minyak atsiri terhadap zona hambat yang dihasilkan (p<0,05). Dari penelitian ini dapat disimpulkan bahwa berbagai konsentrasi minyak atsiri rimpang kunyit dapat menghambat pertumbuhan Candida albicans. Kemampuan ini berpotensi dikembangkan lebih lanjut sebagai alternatif dalam pengobatan terhadap penyakit karena infeksi Candida albicans.

scholarly journals Antifungal Activity of the Essential Oil of Echinops kebericho Mesfin: An In Vitro Study

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Tamirat Bekele Beressa ◽  
Serawit Deyno ◽  
Paul E. Alele
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Cryptococcus Neoformans ◽  
Antioxidant Properties ◽  
Pathogenic Fungi ◽  
Diffusion Method ◽  
Potential Candidate ◽  
Standard Drug

Background. Echinops kebericho is an endemic medicinal plant in Ethiopia widely used in the treatment of infectious and noninfectious diseases. Essential oils are known for their antibacterial, antifungal, antiviral, insecticidal, and antioxidant properties. This study evaluated the antifungal activity of essential oil from E. kebericho against four common pathogenic fungi and two standard strains. Methods. The essential oil was obtained by hydrodistillation. The antifungal screening was done by agar well diffusion method. Minimal inhibitory concentrations (MICs) were determined by broth microdilution. Minimal fungicidal concentrations (MFCs) were determined by subculturing fungal strains with no visible growth onto a Sabouraud dextrose agar (SDA) plate. Results. Candida albicans and Cryptococcus neoformans were highly sensitive while Aspergillus flavus did not show sensitivity up to 1 mg/ml of essential oil; MICs ranged from 0.083 mg/ml to 0.208 mg/ml. Concentration and fungal species showed significant dose-dependent associations ( p < 0.0001 ) with antifungal activity. The MICs of essential oil were comparable to those of the standard drug (fluconazole) against C. glabrata and C. krusei. The lowest MFC of the essential oil was observed against Candida parapsilosis (0.145 mg/ml) while the highest MFC was against Candida krusei (0.667 mg/ml). Conclusion. Echinops kebericho essential oil showed noteworthy antifungal activity against Cryptococcus neoformans, Candida albicans, and Candida glabrata and could be a potential candidate for further antifungal drug development.

scholarly journals Antimicrobial Activity of two Mentha Species Essential Oil and its Dependence on Different Origin and Chemical Diversity

2018 ◽  
Vol 13 (8) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Mária Pl'uchtová ◽  
Teresa Gervasi ◽  
Qada Benameur ◽  
Vito Pellizzeri ◽  
Daniela Grul'ová ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Inhibition Zone ◽  
Chemical Diversity ◽  
Diffusion Method ◽  
Salmonella Spp ◽  
Disk Diffusion Method ◽  
Gram Positive Bacteria ◽  
Growth Inhibition Zone

Genus Mentha presents group of plants which are the most studied in family Lamiaceae. Aboveground parts are used for different purposes in pharmacy, food industry or confectionery. Most important is natural product extracted from leaves - essential oil (EO). The aim of presented experiment was to demonstrate different chemotype and compare antibacterial activity of two Mentha species EO. Plant samples were obtained from various environments – from Slovakia and from Italy. Dominant compounds were determined by GC/MS. The results showed high amount of menthol and menthone in tested Slovak peppermint EO. On the other hand, carvone and 1,8-cineole were determinate as dominant compounds in Italian spearmint EO. The antimicrobial activity of the EO was investigated by disc diffusion and broth micro dilution methods. EO was evaluated for their antibacterial activity against 7 microorganisms: Enterobacter cloacae, Salmonella spp., Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes. The determination results of antibacterial activity by agar disk diffusion method ranged from 7 to 14 mm of the growth inhibition zone. MIC of tested mint EO varied from 0.625 to 2.5 μg/mL. In addition, both EO showed relatively the same antibacterial activity against the selected Gram-negative bacteria. However, there is a variation in the antibacterial activity against Gram-positive bacteria.

scholarly journals Antimicrobial Effects of Egyptian Local Chicory,Cichorium endiviasubsp. pumilum

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Ahmed M. Amer
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Antimicrobial Effect ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Antibiotic Resistant ◽  
Parallel Increase ◽  
Local Plant ◽  
Zones Of Inhibition ◽  
Bacteria And Fungi

The discovery of novel and more efficient antimicrobial agents from natural sources like plants is one of the most important ways through which the growing threat of antibiotic-resistant pathogens can be overcome. Herein, we report the potential antimicrobial activity ofCichorium endiviaL.subsp.pumilum. Different concentrations of various solvent extracts prepared from several parts of chicory were tested for their antimicrobial effect against a panel of microorganisms. The antimicrobial activity was analyzed using the well diffusion method, where zones of inhibition were used as indicators of antimicrobial activity. The results indicated the superiority of seed extracts over both leaf and root extracts. Methanol extracts showed higher activity compared with chloroform and water extracts. Increased solvent extract concentration was accompanied by a parallel increase in the diameter of the inhibition zone. Gram-positive bacteria were found to be more sensitive than Gram-negative bacteria and fungi. On a whole, the highest observed inhibition zones (21.3 ± 0.6 and 20.1 ± 0.4 mm) were recorded with the methanolic extract of chicory seeds againstS. aureusandB. cereus, respectively.These results offer insights into the antimicrobial potency of this Egyptian local plant and provide a basis for further phytochemical and pharmacological research.

scholarly journals In VitroAntimicrobial Activity of Essential Oil ofThymus schimperi,Matricaria chamomilla,Eucalyptus globulus, andRosmarinus officinalis

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Awol Mekonnen ◽  
Berhanu Yitayew ◽  
Alemnesh Tesema ◽  
Solomon Taddese
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Drug Preparation ◽  
Bacteria And Fungi

In this study, thein vitroantimicrobial activities of four plant essential oils (T. schimperi,E. globulus,R. officinalis, andM. Chamomilla) were evaluated against bacteria and fungi. The studies were carried out using agar diffusion method for screening the most effective essential oils and agar dilution to determine minimum inhibitory concentration of the essential oils. Results of this study revealed that essential oils ofT. schimperi,E. globulus, andR. officinaliswere active against bacteria and some fungi. The antimicrobial effect ofM. chamomillawas found to be weaker and did not show any antimicrobial activity. The minimum inhibitory concentration values ofT. schimperiwere<15.75 mg/mL for most of the bacteria and fungi used in this study. The minimum inhibitory concentration values of the other essential oils were in the range of 15.75–36.33 mg/mL against tested bacteria. This study highlighted the antimicrobial activity of the essential oil ofE. globulus,M. chamomilla,T. Schimperi, andR. officinalis. The results indicated thatT. schimperihave shown strong antimicrobial activity which could be potential candidates for preparation of antimicrobial drug preparation.

scholarly journals Antimicrobial activity of Cressa cretica L., a Halophytic Plant

2011 ◽  
Vol 4 (1) ◽  
pp. 203 ◽  
Author(s):  
P. Sunita ◽  
S. Jha ◽  
S. P. Pattanayak ◽  
S. K. Mishra
Keyword(s):  
Candida Albicans ◽  
Aspergillus Niger ◽  
Fusarium Oxysporum ◽  
Candida Tropicalis ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Biochemical Alterations ◽  
Agar Disc ◽  
Aspergillus Fumigates ◽  
Halophytic Plant

In the present investigation different fractions (hexane, ethylacetate and methanol) of the whole methanolic extract of Cressa cretica L.(Convolulaceae), a widely grown halophytic plant were studied against wide ranges of bacteria (both positive and negative strain) and five fungi  Candida albicans, Candida tropicalis – dimorphic fungi, Aspergillus fumigatus, Aspergillus niger – systemic fungi and fusarium oxysporum by agar disc diffusion method. Among the three fractions the ethylacetate fraction of C.cretica showed the highest activity, but among the pathogens highest activity was revealed against Escherichia coli, Klebsiella pneumoniae  (zone of inhibition diameter in mm was found to be 26 and 31, respectively). The ethylacetate fraction was sensitive to both gram +ve and gram –ve bacterias. C.cretica showed higher inhibitory activity against the Aspergillus fumigates, Aspergillus niger (zone of inhibition diameter in mm was found to be 26 and 22, respectively) than the Candida albicans and Candida tropicalis and least was found for Fusarium oxysporum. As ethylacetate fraction revealed better activity than other fractions, further study of morphological and biochemical alterations were carried out only with ethylacetate fraction and the results revealed some morphological and biochemical alterations, which indicates that ethylacetate fraction of C. cretica possess potential broad spectrum antimicrobial activity.Keywords: Cressa cretica; Antifungal; Gram positive bacteria; Gram negative bacteria; Biochemical alterations.© 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v4i1.7719J. Sci. Res. 4 (1), 203-212 (2012)

Ocimum sanctum (L.) essential oil and its lead molecules induce apoptosis in Candida albicans

2014 ◽  
Vol 165 (6) ◽  
pp. 411-419 ◽  
Author(s):  
Amber Khan ◽  
Aijaz Ahmad ◽  
Luqman Ahmad Khan ◽  
Nikhat Manzoor
Keyword(s):  
Candida Albicans ◽  
Essential Oil ◽  
Ocimum Sanctum

scholarly journals The inhibition activity of essential oil and crude extract of nutmeg seed’s (Myristica fragrans Houtt and Myristica fattua Houtt) on the growth of Xanthomonas campestris Oammel from broccoli (Brassica oleracea var. italica)

2003 ◽  
Vol 1 (1) ◽  
pp. 20-24 ◽  
Author(s):  
GALUH SARI KUSUMANINGRUM ◽  
SURANTO SURANTO ◽  
RATNA SETYANINGSIH
Keyword(s):  
Essential Oil ◽  
Brassica Oleracea ◽  
Crude Extract ◽  
Xanthomonas Campestris ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Inhibition Activity ◽  
Disk Diffusion Method ◽  
Myristica Fragrans ◽  
Paper Disk

The aims of this research were to study the inhibition activity of essential oil and crude extract of nutmeg seed (Myristica fragrans and M. fattua) on growth of Xanthomonas campestris which is pathogen to broccoli (Brassica oleracea var. italica), to find out the best concentration of those substances to prevent X. campestris and to compare the inhibition activity of both substances on growth of X. campestris. The essential oil of nutmeg seed (M. fragrans and M. fattua) was obtained by Stahl distillation, while crude extract of the nutmeg seeds was extracted by methanol method. The method of this research was the disk diffusion method on nutrient agar. Paper disk was prestreaked on agar medium with X. campestris to be tested. Each petri disk was placed 5 paper disk dropped with the essential oil and the crude extract of the nutmeg seed in concentrations of 100%, 10%, 1% respectively, while for the comparison, the bactericide Agrept 0,2% was used. The parameters measured were the inhibition zone from the essential oil and the crude extract of the nutmeg seeds. The result of the analysis indicated that the essential oil of M. fragrans effectively inhibited X. campestris starting at concentration of 1%, the essential oil of M. fattua effectively inhibited X. campestris at concentration of 10% and the crude extract of M. fragrans effectively inhibited X. campestris at concentration of 100%. The crude extract of M. fattua was not effective in inhibiting the growth of X. campestris.

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