scholarly journals Antimicrobial activity of Cressa cretica L., a Halophytic Plant

2011 ◽  
Vol 4 (1) ◽  
pp. 203 ◽  
Author(s):  
P. Sunita ◽  
S. Jha ◽  
S. P. Pattanayak ◽  
S. K. Mishra
Keyword(s):  
Candida Albicans ◽  
Aspergillus Niger ◽  
Fusarium Oxysporum ◽  
Candida Tropicalis ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Biochemical Alterations ◽  
Agar Disc ◽  
Aspergillus Fumigates ◽  
Halophytic Plant

In the present investigation different fractions (hexane, ethylacetate and methanol) of the whole methanolic extract of Cressa cretica L.(Convolulaceae), a widely grown halophytic plant were studied against wide ranges of bacteria (both positive and negative strain) and five fungi  Candida albicans, Candida tropicalis – dimorphic fungi, Aspergillus fumigatus, Aspergillus niger – systemic fungi and fusarium oxysporum by agar disc diffusion method. Among the three fractions the ethylacetate fraction of C.cretica showed the highest activity, but among the pathogens highest activity was revealed against Escherichia coli, Klebsiella pneumoniae  (zone of inhibition diameter in mm was found to be 26 and 31, respectively). The ethylacetate fraction was sensitive to both gram +ve and gram –ve bacterias. C.cretica showed higher inhibitory activity against the Aspergillus fumigates, Aspergillus niger (zone of inhibition diameter in mm was found to be 26 and 22, respectively) than the Candida albicans and Candida tropicalis and least was found for Fusarium oxysporum. As ethylacetate fraction revealed better activity than other fractions, further study of morphological and biochemical alterations were carried out only with ethylacetate fraction and the results revealed some morphological and biochemical alterations, which indicates that ethylacetate fraction of C. cretica possess potential broad spectrum antimicrobial activity.Keywords: Cressa cretica; Antifungal; Gram positive bacteria; Gram negative bacteria; Biochemical alterations.© 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v4i1.7719J. Sci. Res. 4 (1), 203-212 (2012)

scholarly journals Antimicrobial activities of certain seaweeds from muddy shore places of kerala

2021 ◽  
Vol 8 (2) ◽  
pp. 31-38
Author(s):  
Selvaraju Raja ◽  
Karuppiah Kannan ◽  
Kalamani Velmurugan ◽  
Samynathan M ◽  
Ephsy K Davis
Keyword(s):  
Candida Albicans ◽  
Aspergillus Niger ◽  
Serratia Marcescens ◽  
Bacillus Licheniformis ◽  
Crude Extract ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Methanol Extracts ◽  
Supernatant Extract

A study was carried out to reveal the growth inhibitory effect of methanol crude extract (MCE) and methanol supernatant extract (MSE) of sea weeds: 1) Gracillaria corticata, 2) Hypnea musciforms, 3) Gelidium micropterum and 4) Hypnea valentiae  against six bacterial pathogens, 1) Pseudomonas aeruginosa, 2) Bacillus licheniformis, 3) Serratia marcescens, 4) Aeromonas hydrophila, 5) Acinetobacter baumanii, 6) Escherichia coli and two fungal strains, 1) Aspergillus niger and 2) Candida albicans respectively. Well diffusion method using zone of inhibition as indicator for growth inhibition was adopted. The results showed that methanol extracts of seaweeds viz., Gracillaria corticata, Hypnea musciforms, and Hypnea valentiae prevented the growth of pathogenic bacteria and fungi. The effect on growth was observed as zone of inhibition, the diameter of which was indicated in the units of a millimeter. The growth of the bacterium, Serratia marcescens was affected by methanol supernatant extract of the three types of seaweeds, Gracillaria corticata, Hypnea musciforms, and Hypnea valentiae, and by the methanolic crude extract of Hypnea musciforms and Hypnea valentiae. However, the growths of other species of bacteria were not controlled by either of the extracts of the seaweeds except, Bacillus licheniformis which was controlled by only Gracillaria corticata. The growth of fungi: Aspergillus niger and Candida albicans were inhibited by the methanol extracts of Gracillaria corticata. Between the two forms of methanolic extracts i.e., supernatant and crude, the efficiency of the supernatant extract was greater than that of crude one. Further, among the three types of seaweeds which showed an effect on the growth of microbes, the level of the zone of inhibition caused by Gracillaria corticata was statistically higher than that of the other two, Hypnea musciforms, and Hypnea valentiae.

scholarly journals Antifungal activity of some medicinal plant extracts against some fungal isolates

2019 ◽  
Vol 7 (1) ◽  
pp. 1-7
Author(s):  
Berivan A. Abdullah ◽  
Diana T. Salih ◽  
Asia A. M. Saadullah
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Fusarium Oxysporum ◽  
Plant Extracts ◽  
Aloe Vera ◽  
Diffusion Method ◽  
Plant Materials ◽  
Fungal Isolates ◽  
Mic Value

Introduction: Fungi live everywhere in the environment, most of them are not dangerous, but some types of fungi can be harmful to humanhealth. The medicinal plants contain many antimicrobial components that make them recently used as powerful drugs. The aim of the present investigation was to examine the antifungal potential and minimum inhibitory concentration (MIC) of three plant extract: Aloe vera gel, cinnamon (Cinnamomum zeylanicum) and turmeric (Curcuma longa) against three fungal species: Aspergillus niger, Candida albicans and Fusarium oxysporum. Materials and Methods: The plant materials were extracted using solvents DMSO and ethanol and then were tested against the selected fungal isolates using well diffusion method. Results and Discussion: Antifungal activity of Aloe vera against Aspergillus niger showed MIC value of 25% whereas for Candida albicans and Fusarium oxysporum the MIC obtained was 100%. Both cinnamon and turmeric showed maximum potency against Aspergillus niger, Candida albicans and Fusarium oxysporum at highest MIC value of 100 %. The degree of inhibition increased correspondingly with increasing concentrations of the plant extracts. Conclusions: the tested plant extracts have an antifungal activity and could be used as alternative drugs.

scholarly journals Synthesis, characterization, and biological evaluation of some 4-((thiophen-2-yl-methylene)amino)benzenesulfonamide metal complexes

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
E. Vijaya Sekhar ◽  
Subhas S. Karki ◽  
Javarappa Rangaswamy ◽  
Mahesh Bhat ◽  
Sujeet Kumar
Keyword(s):  
Escherichia Coli ◽  
Schiff Base ◽  
Aspergillus Niger ◽  
Metal Complexes ◽  
Antimicrobial Properties ◽  
Azomethine Nitrogen ◽  
Diffusion Method ◽  
Sulfa Drugs ◽  
Zone Of Inhibition ◽  
Penicillium Rubrum

Abstract Background Sulfonamides (sulfa drugs) and the metals like mercury, copper, and silver bear antimicrobial properties. The discovery of broad-spectrum antibiotics such as penicillins, cephalosporins, and fluoroquinolones has reduced their use. However, in some instances these drugs are the first-line treatment. The metal-based sulfonamide (e.g., silver sulfadiazine) is considered as first choice treatment in post-burn therapy while the use of silver nanoparticle-cephalexin conjugate to cure Escherichia coli infection explains the synergistic effect of sulfa drugs and their metal conjugates. With growing interest in metal-based sulfonamides and the Schiff base chemistry, it was decided to synthesize sulfonamide Schiff base metal complexes as antioxidant and antimicrobial agent. Results The Fe (III), Ru (III), Co (II), Ni (II), Cu (II), Pd (II), Zn (II), Cd (II), and Hg (II) metal complexes of 4-((thiophen-2-ylmethylene)-amino)-benzenesulfonamide (TMABS) were prepared and studied for thermal stability, geometry, and other electronic properties. The ligand TMABS (Schiff base) and its metal complexes were screened in-vitro for 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and antimicrobial properties against Gram-positive (+ve) Bacillus subtilis (MTCC-441), Staphylococcus aureus (MTCC 7443), Gram-negative (-ve) Escherichia coli (MTCC 40), Salmonella typhi (MTCC 3231), and fungal strains Aspergillus niger (MTCC-1344) and Penicillium rubrum by agar well diffusion method. Results summarized in Tables 3, 4, and 5 represent the inhibitory concentration (IC50) in micromole (μM). The zone of inhibition (ZI) in millimeter (mm) represents antimicrobial properties of TMABS and its metal complexes. Conclusions The synthesized sulfanilamide Schiff base (TMABS) behaved as a neutral and bidentate ligand coordinating with metal ions through its azomethine nitrogen and thiophene sulfur to give complexes with coordination number of 4 and 6 (Fig. 3). The nucleophilic addition of sulfanilamide amino group (–NH2) group to carbonyl carbon (>C=O) of benzaldehyde gave sulfanilamide Schiff base (imine) (Fig. 2). All the metal complexes were colored and stable at room temperature. With IC50 of 9.5 ± 0.1 and 10.0 ± 0.7 μM, the Co, Cu, and Pd complexes appeared better antioxidant than the ligand TMABS (155.3±0.1 μM). The zone of inhibition (ZI) of Hg (28 mm) and Ru complexes (20 mm) were similar to the ligand TMABS (20 mm) against Aspergillus niger (MTCC-1344) as in Figs. 4, 5, and 6. None of the synthesized derivatives had shown better antimicrobial properties than the standard streptomycin sulfate and fluconazole.

scholarly journals Antimicrobial Efficacy of Mineral Trioxide Aggregate against Therapy Resistant Endodontic Microorganisms

2016 ◽  
Vol 33 (3) ◽  
pp. 140-145
Author(s):  
Mst Laila Akter Banu ◽  
AKM Bashar ◽  
Md Mujibur Rahman Howlader ◽  
Md Shamsul Alam ◽  
Md Ashraf Hussain
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Zone Of Inhibition

Microorganisms, usually from the dental caries, are the main sources of diseases in dental pulp (root canals) and periapical region. Facultative bacteria and fungi have been identified in therapy resistant persistent endodontic infection. The objectives of this study was to evaluate the antimicrobial efficacy of Mineral Tri Oxide Aggregate (MTA) against therapy resistant endodontic microorganisms. The efficacy of MTA was also compared with that of calcium hydroxide. Six standard bacterial stains were used: Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, bacillus subtilis, Candida albicans and Enterococcus faecalis. The agar diffusion method on Muller- Hilton media was employed. The plates containing media were inoculated with the specified bacterial suspensions. Two standard holes were prepared on each microorganism inoculated plate with a copper puncher and one hole was completely filled with MTA & the other with Ca (OH)2 . The plates were then kept at environmental temperature for one hour to ensure prediffusion and then incubated at 370C for 24 hours. After 24 hours, the diameters of inhibition zones were measured. Tests were replicated for thirty times for each sample and mean values were taken. Zone of inhibition as measured for MTA and Ca (OH)2 were statistically analyzed with Student’s t-Test and Post Hoc Games Howell Test and were presented as mean ± SD to compare of efficacy of MTA and calcium hydroxide on different microorganisms. Both MTA and Ca(OH)2 were found to produce zone of inhibition against Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853), , Bacillus subtilis (BTCC 17 ), and Candida albicans (BTCC 493). MTA showed highest activity against S. aureus and lowest activity against P. aeruginosa which was similar to the activity range of Ca (OH)2 against the mentioned organisms. But both of them failed to produce any activity against E. coli and. E. faecalis. MTA was found to produce a lower efficacy than Ca (OH)2 while comparing the zone of inhibition between them and statistically it was significant. Mineral Tri Oxide Aggregate (MTA) showed antimicrobial efficacy against some therapy resistant microorganisms but it did not show antimicrobial efficacy against Escherichia coli and Enterococcus faecalis. MTA was found to produce a lower antimicrobial efficacy than Ca (OH)2.J Bangladesh Coll Phys Surg 2015; 33(3): 140-145

scholarly journals Prevalence and Antifungal Susceptibility Pattern of Candida species Isolated from Patients with Urinary Tract Infections

2021 ◽  
Vol 7 (4) ◽  
pp. 328-335
Author(s):  
IA Lawal ◽  
OA Osinupebi ◽  
OV Adeosun
Keyword(s):  
Candida Albicans ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Candida Species ◽  
Antifungal Susceptibility ◽  
Diffusion Method ◽  
Susceptibility Pattern ◽  
Agar Disc ◽  
Sensitivity Pattern ◽  
Tract Infections

Background: The presence of Candida species in the urine is termed candiduria, and it is a common form of urinary tract infection (UTI). Many other species of Candida organism apart from Candida albicans are known with increasing the occurrence of resistance to available antifungal agents. Objectives: To determine the prevalence and sensitivity pattern of Candida isolates obtained from urine samples of diagnosed urinary tract infections. Methods: Midstream urine of patients attending the Lagos University Teaching Hospital, Ikeja Lagos, were collected and inoculated on Sabouraud Dextrose Agar (SDA). Microbiological processing was done with Gram reaction, germ tube test, CHROME agar TM and sugar fermentation test using API 32C system. Antifungal susceptibility tests were done using the agar disc diffusion method. Results: Candida species were obtained from 36 (12.9%) of 280 patients with UTI. Candida albicans (CA) had the highest frequency (12; 33.3%) compared to 24 (66.7%) for Non-albicans Candida (NAC). The speciation of Candida using Chrome Agar showed some misidentification from the API32C identification, but there was a significant correlation between API32C and Chrome Agar methods (r = 0. 9793). Half of the C. albicans species were sensitive to fluconazole while C. hellenica was only sensitive to Nystatin. The C. parapsilopsis had the highest susceptibility pattern, with 86% and 71% for fluconazole and ketoconazole, respectively. Generally, ketoconazole had the highest effectiveness on Candida species. Conclusion: This study demonstrated the role of Candida species in UTIs and their high susceptibility to ketoconazole.

scholarly journals Quantitative Phytochemical Analysis and Antimicrobial Potential of the Ethanol and Aqueous Extracts of the Leaf, Stem and Root of Chromolaena odorata (Asteraceae)

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Ugwoke C E C ◽  
Orji J. ◽  
Anze S P G ◽  
Ilodibia C V
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Infectious Diseases ◽  
Antimicrobial Effect ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Chromolaena Odorata

Background: Plants contain secondary metabolites or phytochemicals, which when consumed by humans give therapeutic effect. This study therefore analyzed the phytochemical composition of Chromolaena odorata so as to give an idea of its possible pharmacological potentials. An antimicrobial assay was also carried out to verify claims on its use in the treatment of infectious diseases. Methods: The experimental procedure involved collection of the leaf, stem and root of the plant from the wild, authenticating the samples and drying under shade to facilitate pulverization. Preliminary qualitative and quantitative phytochemical analyses were done using standard methods to reveal the presence and percentage composition of basic phytochemicals. The powder was also macerated in ethanol and water to produce ethanol and aqueous crude extracts that were reconstituted in normal saline to concentrations (mg/ml) of 150, 100, 75 and 50. Clinical isolates of Staphylococcus aureus, Salmonella typhi, Escherichia coli, Aspergillus niger and Candida albicans were screened for sensitivity to the extracts using the agar well diffusion method. Results: The plant parts contain alkaloids, tannins, flavonoids, saponins, terpenoids, phenols, cardiac glycosides, and sterols at varying concentrations. The leaf however had highest concentration of almost all phytochemicals present. The antimicrobial activity of the plant was concentrationdependent in all parts of the plant and both extracts. Aspergillus niger and Candida albicans were most susceptible while Salmonella typhi and Escherichia coli were least susceptible with an average Inhibition Zone Diameter (IZD) of about 8 – 10 mm. Conclusion: The substantial quantity of the basic phytochemicals in Chromolaena odorata could render it a utility plant in therapeutic use. Due to the profound antimicrobial effect as revealed by the IZDs, the plant could be classified as a broad spectrum antimicrobial agent. Thus, the claim on its potency in treatment of infectious diseases by traditional medical practitioners could be said to be justified.

scholarly journals Antifungal activity of Bacillussp. Gn-A11-18isolated from decomposing solid green household waste in water and soil against Candida albicans and Aspergillus niger

2020 ◽  
Vol 150 ◽  
pp. 02003 ◽  
Author(s):  
Azeddin El Barnossi ◽  
Fatimazahrae Moussaid ◽  
Abdelilah Iraqi Housseini
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Pathogenic Fungus ◽  
Bioactive Compound ◽  
Household Waste ◽  
Diffusion Method ◽  
Agar Plug ◽  
Opportunistic Pathogenic

Candida albicans an opportunistic pathogenic fungus causes many infections in humans. Whereas Aspergillus niger is a fungus that can produce ochratoxins, a group of extremely dangerous secondary metabolites that are classified as potentially carcinogenic to humans and also causing deterioration in grapes, strawberries, etc. The purpose of the current study is to isolate, purify, identify and characterize new microorganisms associated with solid green household waste for the control of C. albicans and A. niger. The antifungal activity of bacterial isolates was carried out in vitro by the agar plug diffusion method, the disk and well diffusion method. The isolate that showed promising activity has been identified by those macroscopic, microscopic and biochemical characteristics. The results obtained in the course of this study showed the isolation of an isolate named Gn-A11-18, which was shown to have significant inhibitory activity with a 42.66% inhibition percentage against A. niger and a 44.66 mm inhibition diameter against C. albicans compared to the controls. The identification of Gn-A11-18 isolate has shown that this isolate belongs to the genus Bacillus with a similarity to Bacillus subtilis and Bacillus tequilensis. In the light of the results of this study, we can suggest that the bioactive compound of Bacillus sp. Gn-A11-18 could become a biological alternate that could have an important role to fight against C. albicans and A. niger.

PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTIMICROBIAL ACTIVITY AGAINST CLINICAL PATHOGEN OF MEDICINALLY IMPORTANT ORCHID CALANTHE MASUCA (D.DON) LINDL

2021 ◽  
pp. 5-8
Author(s):  
V. Nandagopalan ◽  
D. Kavitha
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Petroleum Ether ◽  
Methanol Extract ◽  
Petroleum Ether Extract ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Ether Extract ◽  
Wide Range

Calanthe masuca (D.Done) Lindl is a potential medicinal plant of terrestrial orchids. Tribal peoples were used the plants for therapeutic purpose. The present study was to evaluate the phytochemical analysis and Antimicrobial Activity of Calanthe masuca in leaf extracts. Preliminary phytochemical analysis revealed that the presence of bioactive substance in leaf extract was found that Alkaloids, Flavanoids, Phenol, Tannins, Saponins, Steroids, Terpenoids, Carboxcylic acid, Glycoside and Mucilage. Antimicrobial activity by Agar well diffusion method again six pathogenic microbes such as Staphylococcus aureus, Bacillus subtilis, E.coli and Pseudomonas aeruginosa in bacteria, Aspergillus niger and Candida albicans in fungi by using different solvent such as Aqueous, Methanol and Petroleum ether. It was observered that Methanol extract showed highest activity against the pathogen such as S.aureus (13.5 mm), B.subtilis (12.4 mm), E.coli (12.5 mm) and P.aeruginosa (13.0 mm) and then followed by the Aqueous extract and low activity showed in petroleum ether extract. The methanol extract showed signicant Antifungal activity against Aspergillus niger (14.0mm) and Candida albicans (13.0 mm) with higher concentration and Petroleum ether extract showed lower activity in fungi. The capacity of the crude concentrates of Calanthe masuca to suppress the development of microorganism which means that it has a wide range of antimicrobial potential utilized for various cures.

scholarly journals In vitro antimicrobial activity and phytochemical analysis of Cassia auriculata Linn

2013 ◽  
Vol 2 (6) ◽  
pp. 105-108 ◽  
Author(s):  
Devados Kumarasamy Raja ◽  
Nattanmai Sundararaman Jeganathan ◽  
Rajappan Manavalan
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Pathogenic Bacteria ◽  
Chloroform Extract ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Cassia Auriculata

This study was performed to evaluate the antimicrobial activity of aerial parts of chloroform extract of Cassia auriculata L. The chloroform extract of C. auriculata were shown to possess an antimicrobial activity against two gram positive and two gram negative human pathogenic bacteria and fungi, viz. Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and fungus cultures Candida albicans and Aspergillus niger by using disc diffusion method. The extract showed antibacterial activity at all concentrations selected, but only the extract with the concentration of 300µg/ml showed maximum antibacterial activity against all the organisms except Pseudomonas aeruginosa which are comparable with the standard control, amikacin. The anti fungal activity of chloroform extract of C. auriculata revealed significant effect against Candida albicans and Aspergillus niger with the net inhibition zone of 14 and 14 mm, respectively at 300µg/ml concentration, which is almost comparable with standard control, ketokonazole used as an antifungal agent. The phytochemical analysis showed the presence of alkaloids, carbohydrates, fixed oils, fats, tannins, gum & mucilage, flavonoids, saponins, terpenoids, lignin and sterols. It is concluded that the antimicrobial activity showed by the plant was due to the presence of these phytochemicals. Further studies are highly needed for future drug development.DOI: http://dx.doi.org/10.3329/icpj.v2i6.14869 International Current Pharmaceutical Journal, May 2013, 2(6): 105-108

scholarly journals UJI AKTIVITAS ANTIMIKROBA EKSTRAK DAN FRAKSI KARANG LUNAK (Sarcophyton sp.) DARI PERAIRAN PULAU BANGKA LIKUPANG TERHADAP PERTUMBUHAN MIKROBA Staphylococcus aureus, Salmonella tyhpimurium, DAN Candida albicans

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 470
Author(s):  
Mochamad I. Eda ◽  
Defny S. Wewengkang ◽  
Surya Sumantri
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Salmonella Typhimurium ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Chloroform Fraction ◽  
Disc Diffusion ◽  
Bioactive Materials ◽  
Agar Disc

ABSTRACTSarcophyton sp. is able to produce bioactive materials, the bioactive material contained in Sarcophyton sp., is sarcophine. The purpose of this study is to find out whether the extracts from soft coral Sarcophyton sp., in Bangka Island waters Likupang performed antimicrobial activity against several pathogenic microbes, such as Staphylococcus aureus, Salmonella typhimurium, and Candida albicans. Samples were extracted by maceration method with ethanol solvent and the fractionation method used was liquid-liquid.The antimicrobial activity testing method used in this study is the Kirby and Bauer disc diffusion method. The results obtained from the antimicrobial activity test on Staphylococcus aureus, fractions of n-hexane with result 8.27mm and fractions of chloroform 8.41mm. In Candida albicans , the n-hexane fraction has an 8.18mm and the chloroform fraction 8.06. While for Salmonella typhimurium has no activity. The conclusion of this study it was found that only some fractions have antimicrobial activity and categorized as moderate, such as the fractions of n-hexane and chloroform that only have moderate inhibiting activity against Stapphylococcus aureus and Candida albicans while for Salmonella typhimurium, the extracts and fractions of Sarcophyton sp., has no activity to the way to inhibit these bacteria. Keywords: Sarcophyton sp., Antimicrobial, Staphylococcus aureus, Salmonella tyhpimurium, Candida albicans ABSTRAK Sarcophyton sp. mampu menghasilkan bahan bioaktif, bahan bioaktif yang terdapat pada Sarcophyton sp adalah sarcophine. Tujuan dari penelitian ini yaitu untuk mengetahui apakah ekstrak dari Karang Lunak Sarcophyton sp.,di Perairan Pulau Bangka Likupang memiliki aktivitas antimikroba terhadap beberapa mikroba patogen Staphylococcus aureus, Salmonella typhi, dan Candida albicans. Sampel diekstraksi dengan metode maserasi dengan pelarut etanol dan metode fraksinasi yang digunakan yaitu fraksinasi cair – cair. Metode pengujian aktivitas antimikroba yang digunakan dalam penelitian ini yaitu metode difusi agar (disc diffusion Kirby and Bauer). Hasil yang didapat dari uji aktivitas antimikroba pada bakteri Staphylococcus aureus, fraksi n-heksan mendapatkan hasil 8.27mm dan pada fraksi kloroform 8.41mm. Pada jamur Candida albicans, fraksi n-heksan mendapatkan hasil 8.18mm dan pada fraksi kloroform 8.06mm, sedangkan pada bakteri Salmonella typhi tidak memiliki aktivitas. Kesimpulan dari penelitian ini, hanya sebagian fraksi yang memiliki aktivitas untuk penghambat antimikroba dengan dikategorikan sedang, seperti pada fraksi n-heksan dan klorofom pada bakteri Stapphylococcus aureus dan jamur Candida albicans, sementara untuk bakteri Salmonella tyhpi ekstrak dan fraksi sampel Karang Lunak Sarcophyton sp. tidak memiliki aktivitas untuk menghambat bakteri tersebut. Kata Kunci : Sarcophyton sp., antimikroba, Staphylococcus aureus, Salmonella tyhpimurium, Candida albicans

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