The Influence of Storage in Saline or Irradiation by Ultraviolet on Implant Hydrophilicity and Osseointegration:   An Experimental Study in Rabbits

2021 ◽  
Author(s):  
Yuhao Zhu ◽  
Antian Xu ◽  
Chuan Zhou ◽  
Yefeng Wu ◽  
Guofen Lin ◽  
...  
Keyword(s):  
Uv Irradiation ◽  
Cancellous Bone ◽  
Surface Characterization ◽  
Early Stage ◽  
Healing Time ◽  
Titanium Implants ◽  
Histomorphometric Analysis ◽  
Implant Surface ◽  
Bone Response

Storage in aqueous solution or ultraviolet (UV) irradiation  can  re t ain or regain the hydrophilicity of titanium implant surface.  In this study, t hree types of commercial titanium implants were used : ZBL (ZDI Bone  L evel ® ), CEL (C-tech Esthetic Line ® ) , and modSLA (Straumann SLActive ® ). ZBL and CEL implants were treated with UV irradiation for 4 h. Surface characterization of the four groups (ZBL, ZBL-UV, CEL-UV, modSLA)  was  evaluated by scanning electron microscopy and contact angle measurements. The  in vivo bone response  was  evaluated by removal torque (RTQ) tests and histomorphometric analysis  at  3, 6 , and 12 weeks post-implantation. A total of 144 implants and 36 rabbits were used for experiments according to a previously established randomization sequence. The  ZBL-UV, CEL-UV , andmodSLA groups were hydrophilic, and nanostructures were observed on  the  modSLA implant surface.ModSLA achieved better  RTQ value than ZBL at 12 weeks ( p <0.05). For histomorphometric analysis, ZBL-UV and CEL-UV implants showed higher bone area values in  the  cancellous bone zone at 6 weeks than  did  modSLA and ZBL  implants  ( p <0.05).In  the  cortical bone zone, all groups showed comparable bone-to-implant contact at all healing time points ( p >0.05).Both storage in saline  and UV irradiation could retain or provoke hydr o philic surfaces and improve osseointegration. Compared to storage in saline, UV irradiation displayed slight advantages in promoting new bone formation in cancellous bone zone at  an  early stage.

scholarly journals The Effect of Hydroxyapatite Nanocrystals on Osseointegration of Titanium Implants: AnIn VivoRabbit Study

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Karin Breding ◽  
Ryo Jimbo ◽  
Mariko Hayashi ◽  
Ying Xue ◽  
Kamal Mustafa ◽  
...  
Keyword(s):  
Surface Chemistry ◽  
Calcium Phosphates ◽  
Surface Characteristics ◽  
Healing Time ◽  
Titanium Implants ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Implant Surface ◽  
Hydroxyapatite Nanocrystals

Osseointegration is dependent on implant surface characteristics, including surface chemistry and topography. The presence of nanosized calcium phosphates on the implant surface is interesting to investigate since they affect both the nanotopography and surface chemistry, forming a bone mineral resembling surface. In this work, the osseointegration of titanium implants with and without the presence of hydroxyapatite (HA) nanocrystals has been evaluatedin vivo. The integration was examined using removal torque measurements and real-time polymerase chain reaction (RT-PCR) analysis. The study was performed using two healing time points, 3 and 12 weeks. The results showed that the torque needed to remove the implants was insignificant between the non- and HA-coated implants, both at weeks 3 and 12. The RT-PCR, however, showed significant differences for osteoblast, osteoclast, and proinflammation markers when HA nanocrystals were present.

scholarly journals Histological and Histomorphometric Comparison of Innovative Dental Implants Laser Obtained: Animal Pilot Study

Materials ◽  
2021 ◽  
Vol 14 (8) ◽  
pp. 1830
Author(s):  
Mastrangelo Filiberto ◽  
Botticelli Daniele ◽  
Bengazi Franco ◽  
Scarano Antonio ◽  
Piattelli Adriano ◽  
...  
Keyword(s):  
Pilot Study ◽  
Surface Characteristics ◽  
Objective Evaluation ◽  
Poor Quality ◽  
Histomorphometric Analysis ◽  
Implant Surface ◽  
Bone Response ◽  
Bone To Implant Contact ◽  
Titanium Surfaces

Objective: Evaluation of the in vivo bone response of two innovative titanium surfaces ytterbium laser active fiber obtained (L1-L2) compared to a sandblasted and acid etched (SBAE) during early phase of osseointegration. Material and Methods: Three implant groups with the same macroscopic features were obtained (L1-L2-SBAE) to promote specific surface characteristics. Scanning electron microscopy, profilometric evaluation, X-ray spectrometry, and diffraction analysis were performed. For each group, six implants were placed in the tibiae of three Peli Buey sheep, and histologic, histomorphometric analysis, bone to implant contact (BIC), and the Dynamic Osseointegration index (DOI) were performed. Results: During the early phases of osseointegration, the histological and histomorphometric results showed significant differences between L1-L2-SBAE implants. At 15 and 30 days, histological analysis detected a newly bone formation around all specimens with an higher vital bone in L2 compared to L1 and SBAE both in cortical and in poor-quality marrow bone. At same time, histomorphometric analysis showed significantly higher BIC values in L2 (42.1 ± 2.6 and 82.4 ± 2.2) compared to L1 (5.2 ± 3.1 and 56.2 ± 1.3) and SBAE (23.3 ± 3.9 and 77.3 ± 0.4). DOI medium value showed a higher rate in L2 (2.83) compared to SBAE (2.60) and L1 (1.91). Conclusions: With the limitations of this pilot study, it is possible to assess that the titanium surface characteristics, and not the technologies used to obtain the modification, played a crucial role during the osseointegration process. Histological, histomorphometric, BIC, and DOI evaluation showed a significantly higher rate in L2 specimens compared to others, confirming that the implant surface could increase the bone response in cortical or marrow poor quality bone during the initial phases of osseointegration.

Calcium Phosphate-Coated Titanium Implants in the Mandible: Limitations of the in vivo Minipig Model

2020 ◽  
Vol 61 (6) ◽  
pp. 177-187
Author(s):  
Till Kämmerer ◽  
Tony Lesmeister ◽  
Victor Palarie ◽  
Eik Schiegnitz ◽  
Andrea Schröter ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Statistical Difference ◽  
Surface Modifications ◽  
Titanium Implants ◽  
In Vivo Model ◽  
Implant Surface ◽  
Press Fit ◽  
Endosseous Implant ◽  
Bone To Implant Contact

Introduction: We aimed to compare implant osseointegration with calcium phosphate (CaP) surfaces and rough subtractive-treated sandblasted/acid etched surfaces (SA) in an in vivo minipig mandible model. Materials and Methods: A total of 36 cylindrical press-fit implants with two different surfaces (CaP, n = 18; SA, n = 18) were inserted bilaterally into the mandible of 9 adult female minipigs. After 2, 4, and 8 weeks, we analyzed the cortical bone-to-implant contact (cBIC; %) and area coverage of bone-to-implant contact within representative bone chambers (aBIC; %). Results: After 2 weeks, CaP implants showed no significant increase in cBIC and aBIC compared to SA (cBIC: mean 38 ± 5 vs. 16 ± 11%; aBIC: mean 21 ± 1 vs. 6 ± 9%). Two CaP implants failed to achieve osseointegration. After 4 weeks, no statistical difference between CaP and SA was seen for cBIC (mean 54 ± 15 vs. 43 ± 16%) and aBIC (mean 43 ± 28 vs. 32 ± 6). However, we excluded two implants in each group due to failure of osseointegration. After 8 weeks, we observed no significant intergroup differences (cBIC: 18 ± 9 vs. 18 ± 20%; aBIC: 13 ± 8 vs. 16 ± 9%). Again, three CaP implants and two SA implants had to be excluded due to failure of osseointegration. Conclusion: Due to multiple implant losses, we cannot recommend the oral mandibular minipig in vivo model for future endosseous implant research. Considering the higher rate of osseointegration failure, CaP coatings may provide an alternative to common subtractive implant surface modifications in the early phase post-insertion.

Alkali-Modified Titanium Surface Stimulating Formation of Bone-Implant Interface

2007 ◽  
Vol 361-363 ◽  
pp. 749-752
Author(s):  
J. Strnad ◽  
Jan Macháček ◽  
Z. Strnad ◽  
C. Povýšil ◽  
Marie Strnadová
Keyword(s):  
Titanium Implant ◽  
Surface Characteristics ◽  
Healing Time ◽  
Machined Surface ◽  
Implant Surface ◽  
Bone Implant ◽  
Bone Response ◽  
Modified Surface ◽  
The Difference ◽  
Titanium Implant Surface

This study was carried out to assess the bone response to alkali-modified titanium implant surface (Bio surface), using histomorphometric investigation on an animal model. The mean net contribution of the Bio surface to the increase in bone implant contact (BIC) with reference to the turned, machined surface was evaluated at 7.94 % (BIC/week), within the first five weeks of healing. The contribution was expressed as the difference in the osseointegration rates ( BIC/'healing time) between the implants with alkali modified surface (Bio surface) and those with turned, machined surface. The surface characteristics that differed between the implant surfaces, i.e. surface morphology, specific surface area, contact angle, hydroxylation/hydration, may represent factors that influence the rate of osseointegration.

Histologic Evaluation of a Provisional Implant Retrieved From Man 7 Months After Placement in a Sinus Augmented With Calcium Sulphate: A Case Report

2007 ◽  
Vol 33 (2) ◽  
pp. 89-95 ◽  
Author(s):  
Giovanna Iezzi ◽  
Elisabetta Fiera ◽  
Antonio Scarano ◽  
Gabriele Pecora ◽  
Adriano Piattelli
Keyword(s):  
Close Contact ◽  
Calcium Sulphate ◽  
Titanium Implants ◽  
Implant Surface ◽  
Sinus Augmentation ◽  
Bone Implant ◽  
Native Bone ◽  
Osteocyte Lacunae ◽  
Histologic Evaluation

Abstract Little is known about the in vivo healing processes at the interface of implants placed in different grafting materials. For optimal sinus augmentation, a bone graft substitute that can regenerate high-quality bone and enable the osseointegration of load-bearing titanium implants is needed in clinical practice. Calcium sulphate (CaS) is one of the oldest biomaterials used in medicine, but few studies have addressed its use as a sinus augmentation material in conjunction with simultaneous implant placement. The aim of the present study was to histologically evaluate an immediately loaded provisional implant retrieved 7 months after simultaneous placement in a human sinus grafted with CaS. During retrieval bone detached partially from one of the implants which precluded its use for histologic analysis. The second implant was completely surrounded by native and newly formed bone, and it underwent histologic evaluation. Lamellar bone, with small osteocyte lacunae, was present and in contact with the implant surface. No gaps, epithelial cells, or connective tissues were present at the bone–implant interface. No residual CaS was present. Bone–implant contact percentage was 55% ± 8%. Of this percentage, 40% was represented by native bone and 15% by newly formed bone. CaS showed complete resorption and new bone formation in the maxillary sinus; this bone was found to be in close contact with the implant surface after immediate loading.

scholarly journals Effects of Osseointegration by Bone Morphogenetic Protein-2 on Titanium Implants In Vitro and In Vivo

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Fu-Yuan Teng ◽  
Wen-Cheng Chen ◽  
Yin-Lai Wang ◽  
Chun-Cheng Hung ◽  
Chun-Chieh Tseng
Keyword(s):  
Bone Morphogenetic Protein ◽  
Cell Activity ◽  
Healing Time ◽  
Titanium Implants ◽  
Bone Morphogenetic Protein 2 ◽  
Main Challenge ◽  
Morphogenetic Protein ◽  
Titanium Surfaces

This study designed a biomimetic implant for reducing healing time and achieving early osseointegration to create an active surface. Bone morphogenetic protein-2 (BMP-2) is a strong regulator protein in osteogenic pathways. Due to hardly maintaining BMP-2 biological function and specificity, BMP-2 efficient delivery on implant surfaces is the main challenge for the clinic application. In this study, a novel method for synthesizing functionalized silane film for superior modification with BMP-2 on titanium surfaces is proposed. Three groups were compared with and without BMP-2 on modified titanium surfaces in vitro and in vivo: mechanical grinding; electrochemical modification through potentiostatic anodization (ECH); and sandblasting, alkali heating, and etching (SMART). Cell tests indicated that the ECH and SMART groups with BMP-2 markedly promoted D1 cell activity and differentiation compared with the groups without BMP-2. Moreover, the SMART group with a BMP-2 surface markedly promoted early alkaline phosphatase expression in the D1 cells compared with the other surface groups. Compared with these groups in vivo, SMART silaning with BMP-2 showed superior bone quality and created contact areas between implant and surrounding bones. The SMART group with BMP-2 could promote cell mineralization in vitro and osseointegration in vivo, indicating potential clinical use.

BMP stimulation of bone response adjacent to titanium implants in vivo

1999 ◽  
Vol 10 (3) ◽  
pp. 212-218 ◽  
Author(s):  
Kazuhisa Bessho ◽  
David L. Carnes ◽  
Renee Cavin ◽  
Hsin-yi Chen ◽  
Joo L. Ong
Keyword(s):  
Titanium Implants ◽  
Bone Response ◽  
Stimulation Of

Does surface anodisation of titanium implants change osseointegration and make their extraction from bone any easier?

2008 ◽  
Vol 21 (03) ◽  
pp. 202-210 ◽  
Author(s):  
J. Langhoff ◽  
J. Mayer ◽  
L. Faber ◽  
S. Kaestner ◽  
G. Guibert ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Titanium Implant ◽  
Titanium Implants ◽  
Implant Surface ◽  
Bone Implant ◽  
High Bone ◽  
Titanium Surfaces ◽  
Anodized Titanium ◽  
Titanium Implant Surface

Summary Objectives: Titanium implants have a tendency for high bone-implant bonding, and, in comparison to stainless steel implants are more difficult to remove. The current study was carried out to evaluate, i) the release strength of three selected anodized titanium surfaces with increased nanohardness and low roughness, and ii) bone-implant bonding in vivo. These modified surfaces were intended to give improved anchorage while facilitating easier removal of temporary implants. Material and methods: The new surfaces were referenced to a stainless steel implant and a standard titanium implant surface (TiMAX™). In a sheep limb model, healing period was 3 months. Bone-implant bonding was evaluated either biomechanically or histologically. Results: The new surface anodized screws demonstrated similar or slightly higher bone-implantcontact (BIC) and torque release forces than the titanium reference. The BIC of the stainless steel implants was significant lower than two of the anodized surfaces (p=0.04), but differences between stainless steel and all titanium implants in torque release forces were not significant (p=0.06). Conclusion: The new anodized titanium surfaces showed good bone-implant bonding despite a smooth surface and increased nanohardness. However, they failed to facilitate implant removal at 3 months.

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