Vaccination of broilers against Newcastle disease in the presence of maternally derived antibodies

2017 ◽  
Vol 45 (03) ◽  
pp. 151-158 ◽  
Author(s):  
Máté Halas ◽  
Tamás Süli ◽  
Anto Vrdoljak
Keyword(s):  
Newcastle Disease ◽  
Haemagglutination Inhibition ◽  
Local Immunity ◽  
Live Attenuated Vaccine ◽  
Post Vaccination ◽  
Attenuated Vaccine ◽  
Protection Rate ◽  
Virulent Virus ◽  
Important Means ◽  
Test Points

Summary Objective: The aim of this study was to evaluate the efficacy of a live attenuated vaccine against Newcastle disease in broilers with different levels of maternally derived antibodies (MDA). While vaccination remains the single most important means for controlling Newcastle disease, presence of MDA may interfere with the vaccination of young birds and decrease the efficacy of the vaccine. Materials and methods: Day-old chicks with variable levels of MDA (negative, low and high) were vaccinated with a live attenuated vaccine against Newcastle disease. Three most commonly used inoculation routes were compared; oculonasal, spray and oral (drinking water). Onset and duration of immunity were measured by serology and challenge with virulent virus. Results: Immune response in vaccinated MDA-positive birds was delayed in comparison with SPF controls. Protection was well established already at 14 days post vaccination in SPF birds while in MDA-positive birds it was 1–2 weeks delayed and was lower throughout the study. Non-vaccinated MDA-positive birds lost passive protection completely at 3–4 weeks of age and were significantly more susceptible to challenge than vaccinated hatch mates at all test points. The protection rate increased in vaccinated birds towards the end of the experiment and reached 70–100 % at the last test points (35–42 days of age). Correlation of haemagglutination inhibition (HI) titre vs. protection rate revealed the importance of cellular and local immunity as most of the vaccinated birds with low HI titre were protected, contrary to their unvaccinated hatch mates with the same HI titre. Oculonasal route seems to provide slightly better protection than the other two routes. Conclusions and clinical relevance: Although immune protection in vaccinated MDA-positive birds may be decreased or delayed, vaccination still provides high protection against ND challenge in comparison with the unvaccinated hatch mates. The degree of interference seems to be proportional to the level of MDA. Vaccination schedules therefore need to be designed according to the immune status of the flock.

scholarly journals EFFECT OF PHYLLANTHUS NIRURI L. EXTRACT AS IMMUNOSTIMULATOR ON CHICKEN VACCINATED BY NEWCASTLE DISEASE

2021 ◽  
Vol 22 (1) ◽  
pp. 125-132
Author(s):  
Jola Rahmahani ◽  
Rahaju Ernawati ◽  
Didik Handijatno
Keyword(s):  
Antibody Titre ◽  
Newcastle Disease ◽  
Haemagglutination Inhibition ◽  
Vaccination Program ◽  
Not Given ◽  
Poultry Industry ◽  
Phyllanthus Niruri ◽  
Antibody Synthesis ◽  
Post Vaccination ◽  
Before And After

Newcastle Disease or tetelo is one of main problem in poultry Industry in Indonesia. Prevention such as biosecurity control and routin vaccination program have been conducted to overcome this problem, but they have not given any great impact. Phyllanthus Niruri L. or meniran is well known as immunostimulatory. This research was aimed to reveal effect of Phyllanthus Niruri L. extract on chicken vaccinated with live vaccine LaSota. Administration of Phyllanthus Niruri L. extract was conducted on three different time which were 7 days before vaccination, 1 days after vaccination, and 3 days before and after vaccination. The amount of Phyllanthus Niruri L. extract administered were 2 ml, 2.5 ml, and 3 ml orally. Data of antibody titre were collected for 4 weeks after the treatment. It was obtained by measuring the antibody through Haemagglutination Inhibition test each week. According to the result Phyllanthus Niruri L. extract could increase the amount of antibody titre against Newcastle Disease. The amount of Phyllanthus Niruri L. extract given that capable to induced maximum of antibody titre was administered 1 days after the vaccination with amount 2.5 ml. It is suggested that Phyllanthus Niruri L. extract should be administered post vaccination to boost antibody synthesis.

scholarly journals Humoral response of broilers to live Newcastle Disease virus vaccines manufactured by different companies

2020 ◽  
Vol 10 (3) ◽  
pp. 179-184
Author(s):  
Muhammad Danish Mehmood ◽  
Huma Anwar ◽  
Aamra Hashim ◽  
Muhammad Ismail ◽  
Sajjad Hussain
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Booster Dose ◽  
Haemagglutination Inhibition ◽  
Humoral Response ◽  
Post Vaccination ◽  
Vaccine Schedule ◽  
Vaccination Scheme ◽  
Time Periods

Increased incidence and severity of fatal Newcastle Disease Virus (NDV) in commercial and domestic poultry has been reported from across Pakistan. The present study was carried out to evaluate the efficacy of various live NDV vaccines (Gallivac, Intervet and Ceva) by adapting different vaccination schemes in broilers. Antigenic count of each vaccine and its generated antibody were determined by Haeamagglutination and Haemagglutination inhibition tests respectively. Two different NDV vaccination schemes were tested in such a way that one group had received three vaccines in different time periods whereas, the other after priming at 0 day, was followed by a single booster dose. For this purpose, healthy broilers were divided into four groups A, B, C, and D. On zero day of vaccination, there was no detectable anti NDV-HI titer for all vaccines (Gallivac=2, Intervet=2 & Ceva=2). In first vaccination scheme, detectable anti NDV-HI titer was observed on 16th day of vaccination (Gallivac=3.8, Intervet=4.2 & Ceva=3.6). All vaccines showed protective anti NDV-HI titer on 32nd day, post vaccination (Gallivac=5.8, Intervet=6.2, Ceva=5.8). In second vaccination scheme, detectable anti NDV-HI titer was achieved on 16th day of post vaccination (Gallivac=3.8, Intervet=4.2 & Ceva=3.6). While on 32nd day vaccination, all vaccines showed protective anti NDV-HI titer (Gallivac=5.4, Intervet=5.6 & Ceva=5.2). It is concluded that the two-dose vaccination program, with interval of 12 days, is much effective than the 3-dose vaccination scheme. However, in broilers, anti-NDV antibody titer was induced by all three types of vaccines manufactured by various companies. Keywords: Newcastle disease virus, Humoral Response, Haemagglutination inhibition test, Vaccine schedule

scholarly journals Caution about Newcastle Disease Virus-Based Live Attenuated Vaccine

2008 ◽  
Vol 82 (13) ◽  
pp. 6782-6782 ◽  
Author(s):  
Guan-Zhu Han ◽  
Si-Shen Li ◽  
Xi-Ping Liu
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Live Attenuated Vaccine ◽  
Attenuated Vaccine

scholarly journals Overdose Administration of Thermostable Newcastle Disease Vaccines to Naïve Unvaccinated 6 Weeks Old Cockerels at NVRI, Vom Plateau state, Nigeria

2020 ◽  
pp. 29-34
Author(s):  
A. N. Egbuji ◽  
A. Chukwuedo ◽  
L. N. Shedua ◽  
J. K. Gyallak ◽  
L. N. Swomen ◽  
...  
Keyword(s):  
Newcastle Disease ◽  
Standard Dose ◽  
Haemagglutination Inhibition ◽  
Clinical Signs ◽  
Steady Increase ◽  
Post Vaccination ◽  
Normal Dose ◽  
Veterinary Research ◽  
Plateau State ◽  
Immune Profiling

One hundred and fifty (150) unvaccinated 6 weeks old cockerel were divided into six groups of 25 birds each. Pre-vaccination haemagglutination inhibition (HI) mean titers values of 21.4, <21, 21 <21   21 and 21.6 were observed for groups A, B, C, D E and F respectively. Two batches of Newcastle Disease vaccine (NDVI-2) of 50 and 200 doses produced at National Veterinary Research Institute Vom, with EID50 value of 7.7 and 7.6 per dose were administered to groups B and D at 10x (10 times the normal dose) of NDVI-2 via the intra-ocular and intranasal routes. The rest of the groups were administered normal doses of NDVI-2 through the intra-ocular route. Consequently, 40% of the vaccinated birds were randomly selected and sero-monitored twice at 2 and 4 weeks intervals.  No observable ND clinical signs were seen in both groups B and D irrespective of the vaccine over dosage. Post-vaccination (HI) immune profiling of the vaccinated flock revealed steady increase in the HI mean titer value per group, while groups B and D which had 10x the normal dose showed high response in comparison to groups that had NDVI-2 standard dose. HI immune profile results 2 weeks post-vaccination revealed HI mean titer values of 23.0, 24.0, 23.2 24.2 23.6 and 24.4 for groups A, B, C, D, E and F respectively; week 4 post-vaccination haemagglutination inhibition mean titer values of 23.6, 25.4, 23.0 25.7  25.2 and 24.0 for the respective groups. Week 8 HI mean titer values of 22.2, 21.8, 22.4 22.5 23.2 and   21.8 were equally observed. While week 12 HI mean titer values of 22.0, 21.8, 22.0 22.0 21.8 and 21.8 were recorded. Post-vaccination HI mean titer values showed that groups B and D with 10x the normal dose had no observable adverse clinical signs but rather high ND antibody response was observed in groups where overdose of standard NDVI-2 vaccine were administered and evidence of ND antibody depletion was equally observed in all the vaccinated groups irrespective of the dose administrated. 

scholarly journals The Analysis of Live-Attenuated Piscirickettsia salmonis Vaccine Reveals the Short-Term Upregulation of Innate and Adaptive Immune Genes in Atlantic Salmon (Salmo salar): An In Situ Open-Sea Cages Study

2021 ◽  
Vol 9 (4) ◽  
pp. 703
Author(s):  
Deborah Vargas ◽  
Eva Vallejos-Vidal ◽  
Sebastián Reyes-Cerpa ◽  
Aarón Oyarzún-Arrau ◽  
Claudio Acuña-Castillo ◽  
...  
Keyword(s):  
Immune Response ◽  
Atlantic Salmon ◽  
Salmo Salar ◽  
Cellular Response ◽  
Short Term ◽  
Live Attenuated Vaccine ◽  
Gene Markers ◽  
Post Vaccination ◽  
Atlantic Salmon Salmo Salar ◽  
Piscirickettsia Salmonis

Piscirickettsia salmonis, the etiological agent of the Salmon Rickettsial Septicemia (SRS), is one the most serious health problems for the Chilean salmon industry. Typical antimicrobial strategies used against P. salmonis include antibiotics and vaccines, but these applications have largely failed. A few years ago, the first attenuated-live vaccine against SRS (ALPHA JECT LiVac® SRS vaccine) was released to the market. However, there is no data about the agents involved in the activation of the immune response induced under field conditions. Therefore, in this study we evaluated the expression profile of a set of gene markers related to innate and adaptive immunity in the context of a cellular response in Atlantic salmon (Salmo salar) reared under productive farm conditions and immunized with a live-attenuated vaccine against P. salmonis. We analyzed the expression at zero, 5-, 15- and 45-days post-vaccination (dpv). Our results reveal that the administration of the attenuated live SRS LiVac vaccine induces a short-term upregulation of the cellular-mediated immune response at 5 dpv modulated by the upregulation of ifnα, ifnγ, and the cd4 and cd8α T cell surface markers. In addition, we also registered the upregulation of il-10 and tgfβ. Altogether, the results suggest that a balanced activation of the immune response took place only at early times post-vaccination (5 dpv). The scope of this short-term upregulation of the cellular-mediated immune response against a natural outbreak in fish subjected to productive farm conditions deserves further research.

scholarly journals Immunogenicity and Protective Efficacy of an Intranasal Live-attenuated Vaccine Against SARS-CoV-2

iScience ◽  
2021 ◽  
pp. 102941
Author(s):  
Jun-Guy Park ◽  
Fatai S. Oladunni ◽  
Mohammed A. Rohaim ◽  
Jayde Whittingham-Dowd ◽  
James Tollitt ◽  
...  
Keyword(s):  
Protective Efficacy ◽  
Live Attenuated Vaccine ◽  
Attenuated Vaccine

scholarly journals A Live-Attenuated Prime, Inactivated Boost Vaccination Strategy with Chimeric Hemagglutinin-Based Universal Influenza Virus Vaccines Provides Protection in Ferrets: A Confirmatory Study

Vaccines ◽  
2018 ◽  
Vol 6 (3) ◽  
pp. 47 ◽  
Author(s):  
Raffael Nachbagauer ◽  
Florian Krammer ◽  
Randy Albrecht
Keyword(s):  
Influenza Virus ◽  
Respiratory Tract ◽  
Virus Vaccine ◽  
Influenza Viruses ◽  
Inactivated Vaccine ◽  
Upper Respiratory Tract ◽  
Live Attenuated Vaccine ◽  
Virus Surface ◽  
Virus Challenge ◽  
Attenuated Vaccine

Influenza viruses cause severe diseases and mortality in humans on an annual basis. The current influenza virus vaccines can confer protection when they are well-matched with the circulating strains. However, due to constant changes of the virus surface glycoproteins, the vaccine efficacy can drop substantially in some seasons. In addition, the current seasonal influenza virus vaccines do not protect from avian influenza viruses of human pandemic potential. Novel influenza virus vaccines that aim to elicit antibodies against conserved epitopes like the hemagglutinin stalk could not only reduce the burden of drifted seasonal viruses but potentially also protect humans from infection with zoonotic and emerging pandemic influenza viruses. In this paper, we generated influenza virus vaccine constructs that express chimeric hemagglutinins consisting of exotic, avian head domains and a consistent stalk domain of a seasonal virus. Using such viruses in a sequential immunization regimen can redirect the immune response towards conserved epitopes. In this study, male ferrets received a live-attenuated vaccine virus based on the A/Ann Arbor/6/60 strain expressing a chimeric H8/1 (cH8/1) hemagglutinin, which was followed by a heterologous booster vaccination with a cH5/1N1 formalin inactivated non-adjuvanted whole virus. This group was compared to a second group that received a cH8/1N1 inactivated vaccine followed by a cH5/1N1 inactivated vaccine. Both groups showed a reduction in viral titers in the upper respiratory tract after the A(H1N1)pdm09 virus challenge. Animals that received the live-attenuated vaccine had low or undetectable titers in the lower respiratory tract. The results support the further development of chimeric hemagglutinin-based vaccination strategies. The outcome of this study confirms and corroborates findings from female ferrets primed with a A/Leningrad/134/17/57-based live attenuated cH8/1N1 vaccine followed by vaccination with an AS03-adjuvanted cH5/1N1 split virus vaccine 10.

scholarly journals Newcastle disease virus: is an updated attenuated vaccine needed?

2018 ◽  
Vol 47 (5) ◽  
pp. 467-478 ◽  
Author(s):  
Ehud Shahar ◽  
Ruth Haddas ◽  
Dana Goldenberg ◽  
Avishai Lublin ◽  
Itai Bloch ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Attenuated Vaccine
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