scholarly journals Biosynthesis of Gold Nanoparticles using Scytosiphon lomentaria (Brown algae) and Spyridia filamentosa (Red algae) from Kyrenia Region and Evaluation of their Antimicrobial and Antioxidant Activity

Author(s):  
Doga Kavaz ◽  
Huzaifa Umar ◽  
Tariro Zimuto
Author(s):  
Abhishek Dhanki ◽  
Smit Sindhav ◽  
B. A. Jadeja

Seaweeds are significant marine sources of bioactive compounds with prospective use inefficient foods and nutraceutical products. Four marine macroalgae species from the western coasts of Gujarat were evaluated for their antimicrobial and antioxidant activity. The antimicrobial activity of extracts of red seaweed Hypnea valentiae and Spyridia filamentosa and green seaweed Enteromorpha compressa and Caulerpa racemosa using cold percolation extraction in an in-vitro method and testing against six pathogenic bacteria and one fungi. Results showed effective inhibition zone. The antioxidant activity of the seaweeds methanol extracts was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay for measuring potential scavenger of free radical. Both activities were shown promising result of all four seaweeds. This study suggested that seaweeds could have shown a potential role in the future remedy and nutrition which can be used as drug or food additive.


2016 ◽  
Vol 47 (5) ◽  
pp. 681-687 ◽  
Author(s):  
Viswadevarayalu Annavaram ◽  
Venkata Ramana Posa ◽  
D. Vijaya Lakshmi ◽  
J. Sumalatha ◽  
Adinarayana Reddy Somala

2018 ◽  
Vol 2 (3) ◽  
Author(s):  
Elvi Kustiyah ◽  
Bungaran Saing ◽  
Avira Afriyanti ◽  
Ibnu Susanto Joyosemito

ABSTRAKKandungan dalam alga meliputi polisakarida, mineral, protein, lemak, vitamin, polifenol dan senyawa bioaktif yang bisa memberikan manfaat terhadap sumber makanan, vitamin maupun pharmasi. Investigasi karakter Alga Coklat dan Alga Merah yang diperoleh dari perairan pulau pari kepulauan seribu wilayah Indonesia dilakukan dengan tujuan untuk mendapatkan zat yang mampu menjadi alternatif sumber anti oksidan . Metode yang digunakan dalam penelitian ini adalah ekstraksi maserasi dengan pelarut Phosfat Buffer Saline (PBS) sedangkan Uji kadar protein alga menggunakan metode Lowry dan uji aktivitas antioksidan menggunakan metode 1,1-Diphenyl-2-Pycrylhydrazy(DPPH). Hasil penelitian menunjukkan kadar protein yang tertinggi diperoleh pada sampel alga merah, sedangkan nilai kadar protein paling rendah terdapat pada alga cokelat . Nilai aktivitas antioksidan yang paling baik yaitu pada sampel yang berasal dari alga merah dengan nilai IC50 sebesar 3,5421 mg/ml.Kata Kunci: Alga, protein, antioksidan.ABSTRACTAn alga contains polysaccharides, minerals, proteins, fats, vitamins, polyphenols and bioactive compounds that can provide benefits for food, vitamin and pharmaceutical sources. Characteristic investigations of Brown Algae and Red Algae from the Pari Island waters, Thousand Islands, Indonesia, were carried out to obtain substances that could be an alternative source of antioxidants. The maceration extraction method using Phosphate Buffered Saline (PBS) as solvent was used in this study. Protein content in algae was investigated by Lowry method and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method was used to investigate the antioxidant activity of algae The results showed that the highest protein content was found in Red Algae and the lowest is in Brown Algae. The best value of antioxidant activity was found in Red Algae with the IC50 value of 3.5421 mg/ml.Keywords: Algae, protein, antioxidants.


2020 ◽  
Vol 16 ◽  
Author(s):  
Mojgan Alizadeh ◽  
Akram Arianfar ◽  
Ameneh Mohammadi

Objective: Ziziphora clinopodioides is an edible medicinal plant belongs to the Labiatae family that widespread all over Iran. It used as culinary and also in cold and cough treatments in Iran. The aim of present work was to evaluate the effect of different timeframes during the hydrodistillation on essential oil composition, antimicrobial and antioxidant activity. Materials and Methods: The essential oil of Z. clinopodiodes was extracted via hydrodistillation with Clevenger apparatus. The fractions of essential oil were captured at 6 times from the beginning of the distillation: (10, 20, 60, 120, 180 and 240 min). The fractions of essential oil were analyzed by GC/MS and their antibacterial, antifungal and antioxidant activities were studied by Disk - well diffusion and DPPH methods respectively. Results: Six distillation times and whole essential oil were captured during the hydrodistillation. Essential oil yield dropped off significantly during distillation progressed (1.0% for 10 min and 0.025 for 240 min). 1,8 Cineol, Isomenthone, Pulegone, Piperitenone and Citronellic acid were major compounds in fractions and they were affected by distillation times. Pulegone was major compound in all of essential oils. In antioxidant activity assay, whole essential oil was stronger than was stronger than positive control and fractions of essential oil, because of higher levels of Isomenthone, Piperitenone and Citronellic acid. Strongest antimicrobial activity against S. aureus, E. coli and C. albicans was observed from 10 min fraction. Conclusion: Our results indicated that distillation time can create essential oils with specific properties and we can achieve to more efficient essential oil in short times.


2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
G. Kiran ◽  
T. Maneshwar ◽  
Y. Rajeshwar ◽  
M. Sarangapani

A series of β-Isatin aldehyde-N,N′-thiocarbohydrazone derivatives were synthesized and assayed for theirin vitroantimicrobial and antioxidant activity. The new compounds were characterized based on spectral (FT-IR, NMR, MS) analyses. All the test compounds possessed a broad spectrum of activity having MIC values rangeing from 12.5 to 400 μg/ml against the tested microorganisms. Among the compounds3e,3jand3nshow highest significant antimicrobial activity. The free radical scavenging effects of the test compounds against stable free radical DPPH (α,α-diphenyl-β-picryl hydrazyl) and H2O2were measured spectrophotometrically. Compounds3j,3n,3l, and3e, respectively, had the most effective antioxidant activity against DPPH and H2O2scavenging activity.


Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3193
Author(s):  
Bagda Sagynaikyzy Zhumakanova ◽  
Izabela Korona-Głowniak ◽  
Krystyna Skalicka-Woźniak ◽  
Agnieszka Ludwiczuk ◽  
Tomasz Baj ◽  
...  

The chemical composition of the hydroethanolic extracts (60% v/v) from the aerial parts of Thymus marschallianus Willd (TM) and Thymus seravschanicus Klokov (TS) from Southern Kazakhstan flora was analyzed together with their hexane fractions. Determination of antibacterial, antifungal and antioxidant activities of both extracts was also performed. RP-HPLC/PDA and HPLC/ESI-QTOF-MS showed that there were some differences between the composition of both extracts. The most characteristic components of TM were rosmarinic acid, protocatechuic acid, luteolin 7-O-glucoside, and apigenin 7-O-glucuronide, while protocatechuic acid, luteolin 7-O-glucoside, luteolin 7-O-glucuronide, and eriodictyol predominated in TS. The content of polyplenols was higher in TS than in TM. The GC-MS analysis of the volatile fraction of both examined extracts revealed the presence of thymol and carvacrol. Additionally, sesquiterpenoids, fatty acids, and their ethyl esters were found in TM, and fatty acid methyl esters in TS. The antioxidant activity of both extracts was similar. The antibacterial activity of TS extract was somewhat higher than TM, while antifungal activity was the same. TS extract was the most active against Helicobacter pylori ATCC 43504 with MIC (minimal inhibitory concentration) = 0.625 mg/mL, exerting a bactericidal effect. The obtained data provide novel information about the phytochemistry of both thyme species and suggest new potential application of TS as a source of bioactive compounds, especially with anti-H. pylori activity.


1958 ◽  
Vol 42 (2) ◽  
pp. 243-250 ◽  
Author(s):  
G. C. McLeod

Action spectra for delayed light production by several algae were determined from 250 to 750 mµ incident light. In the visible portion of the spectrum the action spectra resemble those reported by previous workers for photosynthesis and light emission. Blue-green algae had a maximum at 620 mµ, red algae at 550 mµ, whereas green and brown algae have action spectra corresponding to chlorophyll and carotenoid absorption. In the ultraviolet portion of the spectrum delayed light is emitted by algae down to 250 mµ incident light. The action spectra of the different algae are not alike in the ultraviolet portion of the spectrum. This indicates that pigments other than chlorophyll must be sensitizing or shielding the algae in the ultraviolet region.


2014 ◽  
Vol 9 (2) ◽  
pp. 149-155 ◽  
Author(s):  
Mirjana Marčetić ◽  
Silvana Petrović ◽  
Marina Milenković ◽  
Marjan Niketić

AbstractThe chemical composition, antimicrobial and antioxidant activity of Eryngium palmatum, an endemic plant species from the Balkan Peninsula, were investigated. The flavonoids apigenin (9.5±0.3 mg g−1) and apigenin 7-O-glucoside (2.4±0.1 mg g−1) were determined in a methanol extract of aerial parts using HPLC analysis. The methanol extract of roots contained catechin (5.0±0.1 mg g−1), epicatechin (2.9±0.1 mg g−1), chlorogenic acid (1.6±0.0 mg g−1), gallic acid (0.9±0.0 mg g−1) and rosmarinic acid (0.9±0.2 mg g−1). GC-FID and GCMS analysis of a chloroform extract of aerial parts showed that the main volatile constituents were falcarinol, linoleic acid, hexadecanoic acid and methyl linoleate (comprising 32.6%; 24.4%; 19.9; 13.2% of the volatile fraction, respectively), while octanoic acid, tetradecanol and dodecanol dominated in the chloroform extract of the roots (34.9%; 25.8%; 22.2% of the volatile fraction, respectively). Investigation of antimicrobial activity by broth microdilution showed that the methanol and chloroform extracts of aerial parts and roots exerted a significant effect (MIC 3.5–15.6 μg mL−1) against tested Gram-positive and Gram-negative bacteria. The methanol extracts of aerial parts or roots exerted moderate ferric reducing antioxidant power, DPPH radical scavenging activity and hydroxyl radical scavenging activity.


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