scholarly journals Karakterisasi Protein Alga Coklat dan Merah dari Perairan Pulau Pari Sebagai Zat Antioksidan

2018 ◽  
Vol 2 (3) ◽  
Author(s):  
Elvi Kustiyah ◽  
Bungaran Saing ◽  
Avira Afriyanti ◽  
Ibnu Susanto Joyosemito
Keyword(s):  
Antioxidant Activity ◽  
Protein Content ◽  
Red Algae ◽  
Brown Algae ◽  
Alternative Source ◽  
Best Value ◽  
Ic50 Value ◽  
Dpph Method ◽  
Lowry Method ◽  
Thousand Islands

ABSTRAKKandungan dalam alga meliputi polisakarida, mineral, protein, lemak, vitamin, polifenol dan senyawa bioaktif yang bisa memberikan manfaat terhadap sumber makanan, vitamin maupun pharmasi. Investigasi karakter Alga Coklat dan Alga Merah yang diperoleh dari perairan pulau pari kepulauan seribu wilayah Indonesia dilakukan dengan tujuan untuk mendapatkan zat yang mampu menjadi alternatif sumber anti oksidan . Metode yang digunakan dalam penelitian ini adalah ekstraksi maserasi dengan pelarut Phosfat Buffer Saline (PBS) sedangkan Uji kadar protein alga menggunakan metode Lowry dan uji aktivitas antioksidan menggunakan metode 1,1-Diphenyl-2-Pycrylhydrazy(DPPH). Hasil penelitian menunjukkan kadar protein yang tertinggi diperoleh pada sampel alga merah, sedangkan nilai kadar protein paling rendah terdapat pada alga cokelat . Nilai aktivitas antioksidan yang paling baik yaitu pada sampel yang berasal dari alga merah dengan nilai IC50 sebesar 3,5421 mg/ml.Kata Kunci: Alga, protein, antioksidan.ABSTRACTAn alga contains polysaccharides, minerals, proteins, fats, vitamins, polyphenols and bioactive compounds that can provide benefits for food, vitamin and pharmaceutical sources. Characteristic investigations of Brown Algae and Red Algae from the Pari Island waters, Thousand Islands, Indonesia, were carried out to obtain substances that could be an alternative source of antioxidants. The maceration extraction method using Phosphate Buffered Saline (PBS) as solvent was used in this study. Protein content in algae was investigated by Lowry method and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method was used to investigate the antioxidant activity of algae The results showed that the highest protein content was found in Red Algae and the lowest is in Brown Algae. The best value of antioxidant activity was found in Red Algae with the IC50 value of 3.5421 mg/ml.Keywords: Algae, protein, antioxidants.

Ekstraksi Protein dari Rhodophyta dan Chlorophyta Dari Perairan Pulau Pari Sebagai Alternatif Antioksidant

2020 ◽  
Vol 1 (1) ◽  
pp. 24-31
Author(s):  
Elvi Kustiyah ◽  
Bungaran Saingin ◽  
Hernowo Widodo ◽  
Viriya Piti
Keyword(s):  
Antioxidant Activity ◽  
Low Temperature ◽  
Protein Content ◽  
Green Algae ◽  
Red Algae ◽  
Marine Resources ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Lowry Method

 Indonesia has millions island and big part of Indonesia is sea that is rich in marine biological resources and has the potential to be developed and optimized. One of the abundant marine resources in Indonesia is algae. Algae are plant-like protists. Algae have several important compounds, including protein, carbohydrates, fats, minerals and other useful elements. Proteins contained in algae have the potential to be used as antioxidants. In this study, the levels of protein in red and green algae were tested by using the lowry method and testing the antioxidant activity of red and green algae extracts using the Diphenylpicrylhydrazyl (DPPH) method. Algae extraction was done by maceration, which is soaking the sample in low temperature with phosphate buffer saline (PBS) pH 7. From the extraction results it can be concluded that the red algae (Rhodophyta) has the highest protein content of 5.115 ± 0.126% and the lowest protein content in green algae (Chloropytha) as big as 1.686 ± 0.430%. And from the results of the antioxidant activity test showed that all positive algae showed antioxidant activity but the green algae (Chlorophyta) had the highest antioxidant activity of 71.5946 ± 0.01612% with IC50 value 1.6114.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN DAN PENENTUAN TOTAL KANDUNGAN FENOLIK EKSTRAK ETANOL DAUN NANAMUHA (Bridelia monoica Merr)

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 178
Author(s):  
Julia Elsa Lakoro ◽  
Max R. J. Runtuwene ◽  
Paulina V. Y. Yamlean
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Total Phenol ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Phenol Compounds ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ethanol Extracts

ABSTRACTNanamuha (Bridelia monoica Merr.) is a plant that was used as a cancer and tumor drug by the Sangihe Islands community. This plant contains phenol compounds, flavonoids, alkaloids and tannins. The purpose of this study was to determine the potential of nanamuha leaves that grow in the Sangihe Island to have antioxidant activity and determine the total phenolic ethanol extracts of nanamuha leaf. The extraction method used is maceration with ethanol solvent p.a. The extracts obtained was determined total phenolic and antioxidant activity test using DPPH method. Based on the results obtained, the ethanol extracts of nanamuha leaves has antioxidant activity with an IC50 value of 75,03 µg / mL and the result showed that the total phenol content the ethanol extracts of nanamuha leaves of  53,34 mg / L.  Keywords : Nanamuha leaf, total phenol, antioxidant activity, DPPH  ABSTRAKNanamuha (Bridelia monoica Merr.) merupakan tanaman yang dimanfaatkan sebagai obat kanker dan tumor oleh masyarakat Kepulauan Sangihe. Tanaman ini memiliki kandungan senyawa fenol, flavonoid, alkaloid dan tanin. Tujuan dari penelitian ini adalah unutuk mengetahui potensi dari daun nanamuha yang tumbuh di Kepulaun Sangihe ini memiliki aktivitas antioksidan dan menentukan total fenolik dari ekstrak etanol daun nanamuha. Metode ekstraksi yang digunakan adalah maserasi dengan pelarut etanol p.a. Ekstrak yang diperoleh ditentukan total fenolik dan uji aktivitas antioksidan dengan metode DPPH. Berdasarkan hasil yang didapatkan ekstrak etanol daun nanamuha memiliki aktivitas antioksidan dengan nilai IC50 75,03 µg/mL dan hasil penelitian menunjukan total kandungan fenolik ekstrak etanol daun nanamuha sebesar 53,34 mg/L. Kata kunci : Daun Nanamuha, Total fenol, Aktivitas antioksidan, DPPH

scholarly journals Limpasu Pericarpium : an Alternative Source of Antioxidant From Borneo with Sequential Maceration Method

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Irfan Zamzani ◽  
Nita Triadisti
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Oxidation Process ◽  
Antioxidant Activities ◽  
Alternative Source ◽  
Dpph Method ◽  
Indigenous Plant ◽  
Ic50 Values

Antioxidants are substances that can slow down the oxidation process of free radicals. Limpasu plant (Baccaurea lanceolata (Miq) Muell. Arg), an indigenous plant of Borneo, is a natural antioxidant source. The purpose of this study was to determine the antioxidant activity of the limpasu pericarpium extract. The extraction of the limpasu pericarpium was done by maceration method using solvents with increasing polarity ranging from n-hexane, ethyl acetate, and methanol. Antioxidant activities of the three extracts were measured by the DPPH and FRAP methods. The IC50 values of n-hexane, ethyl acetate, and methanol extracts, as well as quercetin using the DPPH method were 517,45 µg/mL, 530,64 µg/mL, 10,63 µg/mL and 6,83 µg/mL, respectively. Meanwhile, the IC50 values obtained from FRAP method were 198,96 µg/mL, 190,07 µg/mL, 661,36 µg/mL, and 7,09 µg/mL, respectively. The results revealed that the methanol extract is more potent than other extracts tested for antioxidant activity.

scholarly journals Anti-inflammatory activity of Jefea gnaphalioides (a. gray), Astereaceae

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Axel Villagómez-Rodríguez ◽  
Julia Pérez-Ramos ◽  
Ana Laura Esquivel-Campos ◽  
Cuauhtémoc Pérez-González ◽  
Claudia Angélica Soto-Peredo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Inflammatory Activity ◽  
Total Phenolic ◽  
Oral Toxicity ◽  
Alternative Source ◽  
Ear Edema ◽  
Tnf Α ◽  
Dpph Method ◽  
Anti Inflammatory ◽  
Commercial Kits

Abstract Background Inflammation is a symptom associated with many diseases. This symptom is treated with steroidal and non-steroidal anti-inflammatory drugs, which can cause severe side effects when used as long-term treatments. Natural products are an alternative source of new compounds with anti-inflammatory activity. Jefea gnaphalioides (Astereaceae) (A. Gray) is a plant species used to treat inflammatory problems, in Mexico. This study determined the anti-inflammatory activity and the composition of the methanol extract of Jefea gnaphalioides (MEJG). Methods The extract was obtained by heating the plant in methanol at boiling point for 4 h, and then the solvent was evaporated under vacuum (MEJG). The derivatization of the extract was performed using Bis-(trimethylsilyl) trifluoroacetamide, and the composition was determined by GC-MS. Total Phenols and flavonoids were determined by Folin-Ciocalteu AlCl3 reaction respectively. The antioxidant activity of MEJG was determined by DPPH method. The acute and chronic anti-inflammatory effects were evaluated on a mouse ear edema induced with 12-O-Tetradecanoylphorbol-13-acetate (TPA). Acute oral toxicity was tested in mice at doses of MEJG of 5000, 2500 and 1250 mg/kg. The levels of NO, TNF-α, IL-1β and IL-6 were determinate in J774A.1 macrophages stimulated by Lipopolysaccharide. The production of inflammatory interleukins was measured using commercial kits, and nitric oxide was measured by the Griess reaction. Results The anti-inflammatory activity of MEJG in acute TPA-induced ear edema was 80.7 ± 2.8%. This result was similar to the value obtained with indomethacin (IND) at the same dose (74.3 ± 2.8%). In chronic TPA-induced edema at doses of 200 mg/kg, the inhibition was 45.7%, which was similar to that obtained with IND (47.4%). MEJG have not toxic effects even at a dose of 5000 mg/kg. MEJG at 25, 50, 100 and 200 μg/mL decreased NO, TNF-α, IL-1β and IL-6 production in macrophages stimulated with LPS. The major compounds in MEJG were α-D-Glucopyranose (6.71%), Palmitic acid (5.59%), D-(+)-Trehalose (11.91%), Quininic acid (4.29%) and Aucubin (1.17%). Total phenolic content was 57.01 mg GAE/g and total flavonoid content was 35.26 mg QE/g MEJG had antioxidant activity. Conclusions MEJG has anti-inflammatory activity.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN SENYAWA PIRAZOLIN 3-(2- METOKSI-FENIL)-5-NAFTALEN-1-IL-4,5-DIHIDRO-1H-PIRAZOL

2018 ◽  
Vol 8 (2) ◽  
pp. 53-58
Author(s):  
Nurlaili ◽  
Jasril ◽  
Hilwan Yuda Teruna
Keyword(s):  
Antioxidant Activity ◽  
Acetic Acid ◽  
Microwave Irradiation ◽  
Biological Activities ◽  
Spectroscopy Data ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Anti Inflammatory

Pyrazoline compounds are reported to have many useful biological activities such as antimicrobials, antihyperglycemia, antioxidants, anti-inflammatory, anticancer and anticonvulsants. The pyrazoline analogues of 3- (2-methoxy-phenyl) -5-naphthalene-1-yl-4,5-dihydro-1H-pyrazol were synthesized usingglacial acetic acid as catalyst and assisted by microwave irradiation. The structures of the compounds were obtainedby UV, IR and HRMS spectroscopy data. Antioxidant activity test using DPPH method showed that pyrazoline compound had IC50 value of 27.41 μg/mL.

scholarly journals SKRINING FITOKIMIA, UJI AKTIVITAS ANTIOKSIDAN, DAN TOKSISITAS DARI EKSTRAK DAUN KERSEN (Muntingia calabura L.) DENGAN METODE 1.1-diphenyl-2-picrylhydrazyl (DPPH) dan Brine Shrimp Lethality Test (BSLT)

PHARMACON ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 315
Author(s):  
Selin Widjaya ◽  
Widdhi Bodhi ◽  
Adithya Yudistira
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Brine Shrimp Lethality ◽  
Phytochemical Content ◽  
Lc50 Value ◽  
Ic50 Value ◽  
Dpph Method ◽  
Muntingia Calabura

ABSTRACTKersen (Muntingia calabura L.) is a plant that has begun to be eliminated and was rarely used because it is often considered to have no economic value and lack of knowledge about its utilization, whereas kersen plants contain flavonoids, saponins, and tannins which were have high benefit for health. The content of metabolites is affected by soil nutrient elements and difference place of growth. This study aims to determine the potential of kersen leaves grown in North Minahasa based on phytochemical content, ability of antioxidant activity, and toxicity. Kersen leaves were extracted using sequential maceration method with n-hexane, ethyl acetate, and ethanol as solvents. Phytochemical Screening using several reagents which tailored to the type of phytochemical test. 1.1-diphenyl-2-picrylhydrazyl (DPPH) method is used to evaluate antioxidant activity, and Brine Shrimp Lethality Test (BSLT) method is used to evaluate toxicity. The result of this study indicate that the n-hexane extract contains phenols, flavonoids, and tannins, with IC50 value 12.54 μg/mL, and LC50 value 881 μg/mL. Ethyl acetate extract contains phenols, flavonoids, tannins, and saponins, with IC50 value 61.3 μg/mL, and LC50 value 1758 μg/mL. Ethanol extract has phenol, flavonoid, tannin, saponin, and terpenoid content, with IC50 value 9.01 μg/mL, and LC50 value 106 μg/mL. Keywords : Kersen leaves, Antioxidant, Toxicity, IC50, LC50 ABSTRAKKersen (Muntingia calabura L.) merupakan tanaman yang sudah mulai tersingkirkan dan jarang dimanfaatkan karena sering dianggap tidak punya nilai ekonomis dan kurangnya pengetahuan tentang pemanfaatannya, padahal tanaman kersen memiliki kandungan flavonoid, saponin, dan tanin yang bermanfaat tinggi untuk kesehatan. Kandungan senyawa metabolit dipengaruhi oleh unsur hara tanah dan perbedaan tempat tumbuh. Penelitian ini bertujuan untuk mengetahui potensi dari daun kersen yang tumbuh di Minahasa Utara berdasarkan kandungan fitokimia, kemampuan aktivitas antioksidan, dan toksisitasnya. Ekstrak daun kersen diekstraksi dengan metode maserasi sekuensial menggunakan pelarut n-heksan, etil asetat, dan etanol.  Skrining fitokimia menggunakan beberapa reagen yang disesuaikan dengan jenis uji fitokimia. Metode 1.1-diphenyl-2-picrylhydrazyl (DPPH) digunakan untuk mengevaluasi aktivitas antioksidan, dan metode Brine Shrimp Lethality Test (BSLT) digunakan untuk mengevaluasi toksisitas. Hasil penelitian ini menunjukkan bahwa ekstrak n-heksan memiliki kandungan fenol, flavonoid, dan tanin, nilai IC50 12,54 μg/mL, dan nilai LC50 881 μg/mL. Ekstrak etil asetat memiliki kandungan fenol, flavonoid, tanin, dan saponin, nilai IC50 61,3 μg/mL, dan nilai LC50 1758 μg/mL. Ekstrak etanol memiliki kandungan fenol, flavonoid, tanin, saponin, dan terpenoid, nilai IC50 9,01 μg/mL, dan nilai LC50 106 μg/mL. Kata kunci : Daun Kersen, Antioksidan, Toksisitas, IC50, LC50

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Daun Ganitri (Elaeocarpus Ganitrus Roxb.) dengan Metode DPPH (2,2 Difenil-1-Pikrilhidazil)

2021 ◽  
Vol 18 (1) ◽  
pp. 60-67
Author(s):  
Naelaz Zukhruf Wakhidatul Kiromah ◽  
Sadam Husein ◽  
Titi Pudji Rahayu
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Synthetic Antioxidant ◽  
Activity Test ◽  
Impaired Liver ◽  
Ic50 Value ◽  
Dpph Method

Free radicals are one of the cause of various diseases. The use of synthetic antioxidant compounds could prevent the effect of the free radicals, however may cause adverse effects on the human body such as impaired liver, lung, intestinal and poisoning. Therefore antioxidant from natural resources needs to be developed. The purpose of this research was to determine the antioxidant activity and IC50 value of the ethanol extract of ganitri (Elaeocarpus ganitrus Roxbs.) leaves. Ganitri leaf ethanol extract activity test was carried out using DPPH method with vitamin C as a standard. Antioxidant activity was determined as a decreas in the absorbance of DPPH at 517 nm wavelength after an addition of the extract with the concentrations of 20, 40, 80, and 100 ppm. The antioxidant acitivity measurement of the ganitri leaf extract showed that the linier regression equation obtained was y = 0.3669x + 29.546, r = 0.4573 while the IC50 value was 54,12 ppm. Based on the result, it is concluded that the ethanol extract of ganitri (Elaeocarpus ganitrus Roxb.) leaf showed was categorized as strong antioxidant.

scholarly journals THE QUALITY PARAMETERS, TOTAL FLAVONOIDS DETERMINATION AND ANTIOXIDANT ACTIVITY COMPOUND OF ANDALIMAN FRUIT ANDALIMAN FRUIT (ZANTHOXYLUM ACANTHOPODIUM DC.) EXTRACT

2021 ◽  
pp. 34-40
Author(s):  
YUNAHARA FARIDA ◽  
WIDI AZELA ◽  
MEGA ELOK LESTAR ◽  
DIAH KARTIKA PRATAMI
Keyword(s):  
Antioxidant Activity ◽  
Methyl Palmitate ◽  
Ethanol Extract ◽  
Quality Parameters ◽  
Total Flavonoid ◽  
Gas Chromatography Mass Spectrometry ◽  
Uv Spectrophotometry ◽  
Benzenedicarboxylic Acid ◽  
Ic50 Value ◽  
Dpph Method

Objective: The aim of this study was to investigate the chemical compound of the antioxidant activity of the extract from andaliman fruit (Zanthoxylum acanthopodium DC.) and the quality parameters and the total flavonoid determination of the highest antioxidant activity. Methods: The andaliman fruits were extracted using a solvent such as n-hexane, ethyl acetate and 70% ethanol obtained extracts, follow the antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The quality parameters and total flavonoid determination were performed from highest antioxidant activity. Fractionated was done using vacuum liquid chromatography (VLC) with the mobile phase chloroform-methanol (9:1 ~1:9). Purification with Thin Layer Chromatography (TLC) preparative, identification using UV-spectrophotometry, FTIR, Gas chromatography Mass Spectrometry (GCMS) analysis. Results: The 70% of ethanol has the highest antioxidant activity with IC50 value 84.11±0.47 ppm; the quality parameters in this study meet the quality requirements, both specific and nonspecific parameters. Total flavonoid content of the 70% ethanol extract was 2.37±0.02%. The results of the GCMS studies showed the presence of 1,2-benzenedicarboxylic acid, mono (2-ethylhexyl) ester; squalene, methyl palmitate, methyl isopalmitate, n-tetracosanol-1 and 4-isopropyl chalcone. Conclusion: The results indicate that the 70% ethanol extracts of andaliman fruits contains 1,2-benzenedicarboxylic acid, mono (2-ethylhexyl) ester; squalene, methyl palmitate, methyl isopalmitate, n-tetracosanol-1 and 4-isopropyl chalcone have antioxidant activity with IC50 value 51.35 ppm.

scholarly journals The Mechanically Extraction Process of Gambier (Uncaria gambier Roxb.) from Limapuluh Kota, West Sumatera and Its Antioxidant activity

2020 ◽  
Vol 9 (1) ◽  
pp. 8-15
Author(s):  
Galuh Widiyarti ◽  
◽  
Andini Sundowo ◽  
Euis Filailla ◽  
Joddy Arya Laksmono ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Trace Amount ◽  
Extraction Process ◽  
Activity Analysis ◽  
Hydraulic Press ◽  
Screw Press ◽  
Ic50 Value ◽  
Dpph Method ◽  
Twin Screw ◽  
Water Contents

The extraction process from leaves and twigs of gambier (Uncaria gambier Roxb)plant was conducted mechanically by using traditional hydraulic press, conventional screw press, and modified twin-screw press. The leaves and twigs of gambier plant was obtained from traditional farmer in Limapuluh Kota, West Sumatera, Indonesia. The water, ash and catechin contents of the gambier extracts were analyzed based on SNI 01-3391-2000 using spectrophotometry and thermogravimetry method. Antioxidant activity analysis of the extracts was also performed by 1,1-diphenyl-2-picrylhidrazyl (DPPH) method and compared tovitamin C as a standard antioxidant. The analysis results showed that the extracts contain no ash. Meanwhile, the catechin and water contents of the extracts were approximately 50 and 13% thus the extracts were classified as quality gambier 2. Other than that, analysis result of catechin and epicatechin contents of the extracts using HPLC and compared to the reference materials showed that catechins contents of the extracts using traditional hydraulic press, conventionalscrew press, and modified twin-screw press give catechin content in about 94.296-95.030%. However, epicatechin was detected in a trace amount. The antioxidant activity of the extracts were 2.5 times stronger than reference. The IC50 value of 4.37-4.52 µg/mL and was categorized as active antioxidant.

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