Comparison of Biomass and Oil Content of Chlorella sp., Nannochloris sp., and Botryococcus braunii in the Mixotrophic Conditions using Glycerol

The biodiesel production has gained increasing attention of the researches in recent years. Current commercial biodiesel production involves transesterification of oil derived from oil crops. Since this production is no more sustainable, the use of microalgae represents a good alternative. Microalgae have high growth rate, high oil content and can be cultured in the environment which are not suitable for agriculture. Additionally, microalgae cultivation improves the reduction of carbon dioxide in the atmosphere. Selection of microalgae for oil production must take into consider certain criteria which include growth rate, oil content, fatty acids profile and ease of separation. In order to analyze the possibility of the use of microalgae for the production of oil, isolation of freshwater microalgae was performed. The isolation was done by the use of traditional techniques from freshwaters near Leskovac. A total number of 6 microalgae strains were isolated and identified as the representatives of the genera Chlorococcum (1 isolate), Chlorella (1 isolate), Scenedesmus (1 isolate) and Desmodesmus (3 isolates). Isolates of microalgae were screened for the growth rate, biomass and oil productivity and oil content. The highest content of biomass was 1.5 g/l and it was observed in the strains Chlorococcum sp. and Desmodesmus sp. 1. The highest value of specific growth rate was calculated during the growth of microalgae Chlorella sp., Scenedesmus sp. and Desmodesmus sp.1, while the lowest value was observed for Chlorococcum sp. Oil productivity was the highest for Chlorella sp. and Desmodesmus sp.1 (0,4 g/l) and the lowest for Desmodesmus sp.2, Desmodesmus sp.3 and Chlorococcum sp. (0,2 g/l). The oil content was in the range from 15.8% (Chlorococcum sp.) to 33% (Chlorella sp). Since the microalgae isolates Chlorella sp. and Scenedesmus sp. had the highest oil productivity, high growth rate and high oil content, these strains are the most suitable for further investigation in order to improve the oil yield and analyse the possibility of the use in the production of biodiesel.

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Biodiesel Production from Dry Microalga Biomass by Microwave-Assisted In-Situ Transesterification

MATEC Web of Conferences ◽

10.1051/matecconf/201815606005 ◽

2018 ◽

Vol 156 ◽

pp. 06005

Author(s):

Lailatul Qadariyah ◽

Mahfud Renova Panjaitan ◽

Fatkhulloh Mujaddid ◽

Ummu Kalsum

Keyword(s):

Reaction Time ◽

Microwave Irradiation ◽

Microwave Power ◽

Oil Content ◽

Catalyst Concentration ◽

Acid Catalyst ◽

Optimum Operating ◽

In Situ Transesterification ◽

Chlorella Sp

Microalga is one of the potential feedstocks in the manufacture of biodiesel because it contains high oil content. In this study, Chlorella sp. was selected because its high oil content about 28-32% of oil (based on its dry weight) and its presence is abundant among other green algae. In situ transesterification was carried out in round neck flask under microwave irradiation. Microwave irradiation can facilitate the in situ transesterification by extracted the lipid of microalga and simultaneous convert to FAME. The purposes of this study are to investigate the effect of acid catalyst concentration, microwave power, reaction time and the addition of co-solvent (n-hexane) on the yield of biodiesel, to get optimum operating conditions and to know the fatty acid compounds of biodiesel from Chlorella sp. The results of oil extraction and biodiesel were analyzed by GC-MS analysis. Based on the experiment, the yield of microalga oil was 11.37%. The optimum yield of biodiesel by in-situ transesterification was 75.68%. It was obtained at the microwave power of 450 watts, the reaction time of 60 minutes, an acid catalyst concentration of 0,2M of H2SO4, and the co-solvent addition of 10 ml.

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Radiation Characteristics of Promising Algae for CO2 Fixation and Biofuel Production

Volume 1: Heat Transfer in Energy Systems; Thermophysical Properties; Heat Transfer Equipment; Heat Transfer in Electronic Equipment ◽

10.1115/ht2009-88019 ◽

2009 ◽

Author(s):

Halil Berberog˘lu ◽

Pedro S. Gomez ◽

Laurent Pilon

Keyword(s):

Cross Section ◽

Co2 Fixation ◽

Life Support ◽

Botryococcus Braunii ◽

Biofuel Production ◽

Chlorophyll B ◽

Radiation Characteristics ◽

Chlorella Sp ◽

Scattering Phase ◽

Scattering Phase Function

This paper reports experimental measurements of the radiation characteristics of green algae used for carbon dioxide fixation via photosynthesis. Particular attention was paid to three widely used species namely Botryococcus braunii, Chlorella sp., and Chlorococcum littorale. Their extinction and absorption coefficients were obtained from normal-normal and normal-hemispherical transmittance measurements over the spectral range from 400 to 800 nm. Moreover, a polar nephelometer is used to measure the scattering phase function of the microorganisms at 632.8 nm. It was observed that for all strains, scattering dominates over absorption. The magnitudes of the extinction and scattering cross-section are functions of the size, shape, and chlorophyll content of each strains in a non-trivial manner. Absorption peaks at 435, 475, and 676 nm corresponding to chlorophyll a and chlorophyll b have been clearly identified in the three species considered. The results can be used for scaling and optimization of CO2 fixation in ponds or photobioreactors as well as in the development of controlled ecological life support systems.

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Biodiesel production from microalgae Botryococcus braunii

SQUALEN Bulletin of Marine and Fisheries Postharvest and Biotechnology ◽

10.15578/squalen.v5i1.43 ◽

2010 ◽

Vol 5 (1) ◽

pp. 23

Author(s):

Sriamini Sriamini ◽

Rini Susilowati

Keyword(s):

Energy Source ◽

Oil Content ◽

Renewable Energy Sources ◽

Dry Weight ◽

Botryococcus Braunii ◽

Biodiesel Production ◽

Energy Needs ◽

New Energy ◽

Chemical Products ◽

Petroleum Fuels

Increasing energy needs cause diminishing energy resources. This encourages the searchfor renewable energy sources to anticipate scarcity. One of the new energy source is microalgae.Microalgae have a high variation of species and have a great potential to be developed as foodand other chemical products. Microalgae has been developed as a potential source of biodieselto replace petroleum fuels derived from foss ils. Of several microalgae s pecies studied,Botryococcus braunii produces the largest oil content, i.e. 75% dry weight. This paper describessteps of producing oil from B. brauniiwhich includes preparation of microalgae biomass,biomass harvesting, and extraction of oil. Oil content of B. braunii is composed mostly ofhydrocarbons (± 15–76% by dry weight), called botryococcene. This type of hydrocarbon ispotential as an energy source of biodiesel.

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MATHEMATICAL MODELLING OF DRYING OF CHLORELLA SP., NEOCHLORIS CONJUNCTA AND BOTRYOCOCCUS BRAUNII AT DIFFERENT DRYING CONDITIONS

European Journal of Sustainable Development ◽

10.14207/ejsd.2016.v5n4p421 ◽

2016 ◽

Vol 5 (4) ◽

Cited By ~ 3

Author(s):

Sevil Karaaslan ◽

Onder Uysal ◽

F. Ozge Uysal ◽

Kamil Ekinci ◽

B. Salih Kumbul

Keyword(s):

Mathematical Modelling ◽

Botryococcus Braunii ◽

Chlorella Sp ◽

Drying Conditions

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Decreasing Levels of BOD, COD and Oil in Palm Oil Mill Effluent (Pome) Using Scale Up Experiment of Symbiosis Mutualism Technology Between Microalgae Chorella Sp and Agrobost

Journal of Applied Agricultural Science and Technology ◽

10.32530/jaast.v4i2.155 ◽

2020 ◽

Vol 4 (2) ◽

pp. 170-180

Author(s):

Yelmira Zalfiatri ◽

Fajar Restuhadi ◽

Angga Pramana ◽

Fajar Yuliandri

Keyword(s):

Palm Oil ◽

Oil Content ◽

Scale Up ◽

Palm Oil Mill Effluent ◽

Chlorella Sp ◽

Randomized Design ◽

Symbiotic Mutualism ◽

Total Treatment ◽

Sago Waste ◽

Palm Oil Mill

Biological processing liquid sago waste is carried out by utilizing microlagae which are symbiotic with decomposer bacteria. The aim of this research was to get chosen treatment of microalgae Chlorella sp. as a reducing BOD, COD and Oil content in Palm Oil Mill Effluent with symbiotic mutualism between microalgae Chorella sp and Agrobost. This research used a Complete Randomized Design (CRD) with treatment 5 times and replication 3 times. This research was microalgae 800 ml/L (with abundance 6.883.000 cell/ml) in 6000 ml of total treatment solution with 5 treatments of Agrobost (0% v/v, 4% v/v, 8% v/v, 12% v/v, and 16% v/v). Data processing using ANOVA and DNRT 5%, the results showed that the concentration of Agrobost had significant affects for BOD, COD, oil and pH. The chosen treatment from the result of this research was 16% v/v which had the value BOD 89.10 mg/L, COD 297.67 mg/L, oil 2.85 mg/L, and pH 9.05.

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