scholarly journals Is there an aesthetics in golden ratio as regards to the common cis-regulatory elements versus to atomic numbers of elements with respect to Quantum perspective model?

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Tahir Ölmez
Keyword(s):  
Golden Ratio ◽  
Regulatory Elements ◽  
The Common ◽  
Perspective Model

Diminished Mobilization of Self-Inactivating (SIN) Lentiviral Vectors Containing Globin Regulatory Elements Compared to Those Containing a Retroviral Long Terminal Repeat.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 5271-5271
Author(s):  
Hideki Hanawa ◽  
Derek A. Persons ◽  
Takashi Shimada ◽  
Arthur W. Nienhuis
Keyword(s):  
Stem Cell ◽  
Long Terminal Repeat ◽  
Hela Cells ◽  
Lentiviral Vector ◽  
Severe Combined Immunodeficiency ◽  
Lentiviral Vectors ◽  
Regulatory Elements ◽  
Terminal Repeat ◽  
Vector Genome ◽  
The Common

Abstract Stem cell transfer was successful in treating severe combined immunodeficiency due to deficiencies of the common γ-chain of the lymphoid cytokine receptor (N Engl J Med346:1185, 2002) and adenosine deaminase (Science296:2410, 2002) but 2 of 10 children in the common γ-chain trial developed a lymphoproliferative disease secondary in part due to activation of the LMO2 proto-oncogene by the retroviral long terminal repeat (LTR) (Science302:415, 2003). Both oncoretroviral and lentiviral vectors integrate preferentially into transcriptionally active genes so that vector design to improve safety is important. In focusing on the interactions between vector sequences and the genome surrounding integration sites, we have used self-inactivating (SIN) lentiviral vectors with transcriptionally inactive LTRs. One assay detects mobilization of the vector genome by rescue of a GFP marker while a second detects vector encoded tat transcription (Blood102:249a, 2003). Approximately 1 in 1,000 to 1 in 3,000 vector genomes containing the Mouse Stem Cell Virus (MSCV) LTR are transcribed with transcription arising from cryptic promoters within the vector genome or from nearby genomic sequences. The frequency of genome transcription was diminished by removal of the MSCV LTR enhancer or by addition of an insulator element from the β-globin locus control region (LCR) to the lentiviral SIN LTR. We have now evaluated the effect of globin regulatory elements on transcription of integrated SIN lentiviral vector genomes. Initially, we substituted the LTR GFP cassette in vector MSCV-U3 for one in which elements from the β-globin LCR were linked to the β-globin promoter driving GFP in the reverse transcriptional orientation (d432βGFPim). Two additional vectors were also studied; the globin LCR β-promoter GFP cassette was reversed and the globin RNA processing signals removed to derive vector Fd432βGFP and then it was modified by substituting larger LCR elements to derive vector FmLARβV5GFP. Vector preparations made by co-transfection of 293T cells with vector and packaging plasmids had transducing titers from 1.8 x 107 to 3.0 x 107 TU/ml as assayed on HeLa cells. Three separate polyclonal 293T cell populations transduced 3 times at high vector concentration had copy numbers of the SIN-proviral genomes, as measured by RealTime PCR, that averaged 41 with a range of 23–68. Vector mobilization was evaluated by transfection of these populations with packaging plasmids. Conditioned media harvested from the transfected cells were then assayed for transfer of the GFP marker on HeLa cells. The mobilized titers were normalized based on vector copy number. The mobilized titer of the MSCV-U3 was 38,000 ± 3500 and that of d432βGFPim was only 810 ± 100 (p = 0.0004). The mobilized titers of the vectors in which the globin regulatory elements were in the forward orientation were also significantly lower than MSCV-U3; Fd432βGFP was 2100 ± 250 (p = 0.005) and FmLARβV5GFP was 1600 ± 120 (p = 0.0005). Those data suggest that the globin LCR elements are less likely than the retroviral LTR to induce transcription of the integrated vector genome in nonerythroid cells. Our results combined with ongoing analysis of the influence of vector integration on expression of surrounding genes in separate studies will provide a safety profile of globin lentiviral vectors to guide the development of future clinical protocols.

scholarly journals Is there a Similiarity between Fibonacci Sequence and Euler’s Number with Respect to Quantum Perspective Model?

2021 ◽  
pp. 35-39
Author(s):  
Tahir Ölmez
Keyword(s):  
Golden Ratio ◽  
Fibonacci Sequence ◽  
Point Of View ◽  
Base System ◽  
Fibonacci Series ◽  
Perspective Model ◽  
First Time

According to Quantum Perspective Model, this article studies whether there is a link between the Euler’s numbers and the Fibonacci series. When the digits of the Euler’s number after the comma are converted from decimal(10) number base system to binary(2) number base system, it corresponds to the number in the Fibonacci series.(0,1,1,2,3,5,8,13,21,34,55...)[7].From this point of view, when the first hundred digits of the Euler’s numbers after the comma were calculated, the number "55" (ten times) in the Fibonacci series was found, in particular. Besides, the eleventh number in the Fibonacci series is also "55".In other words, the approximate unchanged numbers of the golden ratio numbers after the comma can be reached for the first time after dividing them from “55” to “34” (1,618). In sum, Euler’s numbers are not only attributed to the Fibonacci series in mathematics, but also attributed to the golden ratio in nature.

scholarly journals Word and melody in the opus of Momcilo and Svetomir Nastasijevic

Muzikologija ◽  
2013 ◽  
pp. 91-104
Author(s):  
Vesna Peno
Keyword(s):  
Common Ground ◽  
Golden Ratio ◽  
Movie Industry ◽  
Music Drama ◽  
The World ◽  
Poetic Expression ◽  
Universal Values ◽  
The Common ◽  
Artistically Gifted ◽  
Mass Entertainment

In the house of artistically gifted family Nastasijevic where renowned Belgrade artists used to gather together during the twenties and thirties of the previous century, music was nothing but an excuse for regular meetings. The art of sound represented a backbone of one system of poetics in the shaping of which Nastasijevic brothers as well as their close contemporaries and faithful friends truly found the common ground. Rethinking the music phenomenon brought them close to their otherwise remote relatives by thought - to Greek philosophers, but also to French and especially Russian symbolists whose opinions they shared. A pursue for, on one hand, an authentically native but, on the other, cosmopolitan spirit and universal values, marked the creative output of Momcilo, a writer and a pivot of Nastasijevic family, but also the overall creative engagement of his brothers. They all shared common aesthetic standards, attempting to apply these in their respective artistic media as well as in synergy, in their joint ventures. In music dramas entitled Medjulusko blago and Djuradj Brankovic, for which Momcilo wrote the script and Svetomir composed the music, the brothers tried to transform into deeds the Nastasijevic-like convictions regarding music. The aim was to re-find and turn a native - homeland melody into sound. This melody represented a ?golden ratio point? in which all arts meet; it is a kind of a spiritual totality embodying one form of spiritual existence which cannot be reached otherwise but from the ?homeland soil?. They believed they would reach the archetype of national identity and, at the same time, of the universal being by harking at and perpetual crying for the forgotten sound of words. Momcilo?s poetic concept demanded necessary interventions in respect to the dramatic genre. In his essay Dramsko stvaralastvo i pozoriste kod nas, he unequivocally opposed to what he argued to be a decadent and artistically senseless Serbian stage, a place wherefrom an individual and the world he lives in are being superficially analyzed, a place that only serves for mass entertainment, propagation of political and social convictions, and which in its character is getting closer to movie industry. By discarding realistic proc?d?, the poet reached for a myth in Medjulusko blago whereas in Djuradj Brankovic he turned to historical themes. In both of these he delved deep in the essence of drama. His poetic expression had therefore to become dense, more a premonition than a statement, so that it necessarily needed melody as an attribute. Music is a pivot of Medjulusko blago, which Nastasijevic brothers emphasized to be not an opera, or Wagnerian music drama, but a drama meant for being interpreted through singing and nothing but singing. The drama is a music one because the very storyline and the characters? words were supposed to emanate music expression, and music was to drive the characters to a tragic conflict and actualize the antique fatum that permeated, resonated in and guided all creatures. The words and the melody were meant to be inseparable in Djuradj Brankovic music drama as well. To what extent their music works actualized what the ?Nastasijevic manifesto? theoretically proclaimed is to be left to be considered in some other topical study.

Catalogue reduction techniques

1978 ◽  
Vol 48 ◽  
pp. 389-390 ◽  
Author(s):  
Chr. de Vegt
Keyword(s):  
Adjustment Process ◽  
Proper Motions ◽  
Measuring Process ◽  
Aerial Photogrammetry ◽  
Reduction Techniques ◽  
Observational Technique ◽  
The Common ◽  
Astrometric Data

AbstractReduction techniques as applied to astrometric data material tend to split up traditionally into at least two different classes according to the observational technique used, namely transit circle observations and photographic observations. Although it is not realized fully in practice at present, the application of a blockadjustment technique for all kind of catalogue reductions is suggested. The term blockadjustment shall denote in this context the common adjustment of the principal unknowns which are the positions, proper motions and certain reduction parameters modelling the systematic properties of the observational process. Especially for old epoch catalogue data we frequently meet the situation that no independent detailed information on the telescope properties and other instrumental parameters, describing for example the measuring process, is available from special calibration observations or measurements; therefore the adjustment process should be highly self-calibrating, that means: all necessary information has to be extracted from the catalogue data themselves. Successful applications of this concept have been made already in the field of aerial photogrammetry.

Renilla Bioluminescence: A Morphologic Approach to the Location of Photocytes

1974 ◽  
Vol 32 ◽  
pp. 208-209
Author(s):  
Ben O. Spurlock ◽  
Milton J. Cormier
Keyword(s):  
Excited State ◽  
Ground State ◽  
Molecular Basis ◽  
Light Emission ◽  
Chemical Basis ◽  
Electronic Excited State ◽  
Colonial Form ◽  
The Common ◽  
Eighteenth And Nineteenth Centuries ◽  
Catalyzed Oxidation

The phenomenon of bioluminescence has fascinated layman and scientist alike for many centuries. During the eighteenth and nineteenth centuries a number of observations were reported on the physiology of bioluminescence in Renilla, the common sea pansy. More recently biochemists have directed their attention to the molecular basis of luminosity in this colonial form. These studies have centered primarily on defining the chemical basis for bioluminescence and its control. It is now established that bioluminescence in Renilla arises due to the luciferase-catalyzed oxidation of luciferin. This results in the creation of a product (oxyluciferin) in an electronic excited state. The transition of oxyluciferin from its excited state to the ground state leads to light emission.

Mammalian Bone Marrow Acetylcholinesterase

1982 ◽  
Vol 40 ◽  
pp. 44-45
Author(s):  
Ezzatollah Keyhani
Keyword(s):  
Central Nervous System ◽  
Bone Marrow ◽  
Nervous System ◽  
Common Property ◽  
Extracellular Medium ◽  
The Central Nervous System ◽  
The Common ◽  
Mammalian Bone

Acetylcholinesterase (EC 3.1.1.7) (ACHE) has been localized at cholinergic junctions both in the central nervous system and at the periphery and it functions in neurotransmission. ACHE was also found in other tissues without involvement in neurotransmission, but exhibiting the common property of transporting water and ions. This communication describes intracellular ACHE in mammalian bone marrow and its secretion into the extracellular medium.

Correlation analysis of radiation damage

1978 ◽  
Vol 36 (1) ◽  
pp. 214-215
Author(s):  
R. Hegerl ◽  
A. Feltynowski ◽  
B. Grill
Keyword(s):  
Electron Microscopy ◽  
Independent Random Variables ◽  
Optical Parameters ◽  
Bright Field Image ◽  
Bright Field ◽  
Expectation Value ◽  
All Optical ◽  
Image Element ◽  
Stochastic Series ◽  
The Common

Till now correlation functions have been used in electron microscopy for two purposes: a) to find the common origin of two micrographs representing the same object, b) to check the optical parameters e. g. the focus. There is a third possibility of application, if all optical parameters are constant during a series of exposures. In this case all differences between the micrographs can only be caused by different noise distributions and by modifications of the object induced by radiation.Because of the electron noise, a discrete bright field image can be considered as a stochastic series Pm,where i denotes the number of the image and m (m = 1,.., M) the image element. Assuming a stable object, the expectation value of Pm would be Ηm for all images. The electron noise can be introduced by addition of stationary, mutual independent random variables nm with zero expectation and the variance. It is possible to treat the modifications of the object as a noise, too.

The Red Neuronal Pigment in the Nervous System of the Mussel, Mytilus Edulis: Localization and Its Effect on Lateral Ciliary Activity with Spectral and Analytical Changes

1981 ◽  
Vol 39 ◽  
pp. 494-495
Author(s):  
Anthony A. Paparo ◽  
Judith A. Murphy
Keyword(s):  
Nervous System ◽  
Mytilus Edulis ◽  
Neuronal Cell ◽  
Ciliary Activity ◽  
Neuronal Cell Bodies ◽  
The Central Nervous System ◽  
Intracellular Organelles ◽  
The Common ◽  
The Individual ◽  
Cryostat Sections

The purpose of this study was to localize the red neuronal pigment in Mytilus edulis and examine its role in the control of lateral ciliary activity in the gill. The visceral ganglia (Vg) in the central nervous system show an over al red pigmentation. Most red pigments examined in squash preps and cryostat sec tions were localized in the neuronal cell bodies and proximal axon regions. Unstained cryostat sections showed highly localized patches of this pigment scattered throughout the cells in the form of dense granular masses about 5-7 um in diameter, with the individual granules ranging from 0.6-1.3 um in diame ter. Tissue stained with Gomori's method for Fe showed bright blue granular masses of about the same size and structure as previously seen in unstained cryostat sections.Thick section microanalysis (Fig.l) confirmed both the localization and presence of Fe in the nerve cell. These nerve cells of the Vg share with other pigmented photosensitive cells the common cytostructural feature of localization of absorbing molecules in intracellular organelles where they are tightly ordered in fine substructures.

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