scholarly journals Molecular and genetic analysis of Mycobacterium tuberculosis population in the Vologda Region with low tuberculosis incidence

2021 ◽  
Vol 11 (3) ◽  
pp. 497-505
Author(s):  
А. A. Vyazovaya ◽  
I. A. Lebedeva ◽  
N. B. Ushakova ◽  
V. V. Pavlov ◽  
A. A. Gerasimova ◽  
...  
Keyword(s):  
Multiple Drug Resistance ◽  
Molecular Genetic ◽  
Federal District ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Beijing Genotype ◽  
Multiple Drug ◽  
Molecular Genetic Study ◽  
Vologda Region ◽  
Central Asian

The Vologda Region is characterized by a relatively calm epidemic situation for tuberculosis in Russia: the incidence rate in 2010—2018 is decreased from 45.2 to 15.8 per 100 thousand of the population (44.4 in Russia). However, the proportion of patients with multiple drug resistance (MDR) of the pathogen increased from 12.1% in 2016 to 23.7% in 2018. The aim of the study was to characterize the genetic structure of the M. tuberculosis population and identify the main genotypes associated with the primary multidrug resistance of the pathogen in the Vologda Region. A total of 82 strains of M. tuberculosis isolated in 2018 from newly diagnosed tuberculosis patients were studied. Drug susceptibility testing was performed using the standard method of absolute concentration and BACTEC MGIT 960 kit. M. tuberculosis strains were assigned to the Beijing genotype and its main subtypes based on the analysis of specific markers. The Beijing strains were subtyped by the MIRU-VNTR method (24 standard loci), calculating the Hunter-Gaston Discriminatory Index (HGDI). Other strains of the non-Beijing group were spoligotyped. The majority of the strains were of the Beijing genotype (62.2%; 51 of 82). The most numerous cluster was Central Asian/Russian (41.5%; 34 of 82 strains). The shares of the Central Asia Outbreak (CAO) subtype and cluster B0/W148 amounted to 8.5% and 7.3%, respectively. The non-Beijing strains belonged to the genetic families T (11%; 9 of 82), LAM (11%), Haarlem (6.1%), and Ural (4.9%). Among 82 M. tuberculosis isolates, 33 (40.2%) MDR strains were identified, counting 27 of the Beijing genotype, including those of the Central Asian/Russian — 18 (66.7%), B0/W148 and CAO — 4 each (14.8%) clusters. MIRU-VNTR typing of 51 Beijing strains revealed 22 profiles (HGDI = 0.852); the largest clusters were 94-32 (35.3%) and 95-32 (15.7%), which included strains Central Asian/Russian and CAO. Four strains of genotype B0/W148 belonged to cluster 100-32. The loci QUB26 (HGDI = 0.493) and MIRU26 (HGDI = 0.388) had the highest polymorphism. For the first time, a molecular genetic study carried out in the Vologda region revealed the heterogeneity of the M. tuberculosis population with strains of the Beijing genotype dominated. At the same time, the share of the associated with MDR, epidemiologically and clinically significant cluster Beijing B0/W148, well defined in Russia and abroad, was only 7.3%, which is significantly less than in other regions of the Northwestern Federal District of the Russian Federation (~19%). Concurrent, representatives of the Central Asian/Russian cluster of the Beijing genotype prevailed in the structure of genotypes and among MDR M. tuberculosis strains.

scholarly journals Biological risk factor of occupational morbidity with tuberculosis among medical professionals

2018 ◽  
pp. 52-57
Author(s):  
O. A. Pasechnik ◽  
O. V. Plotnikova ◽  
V. L. Stasenko ◽  
M. A. Dymova
Keyword(s):  
Risk Factor ◽  
Multiple Drug Resistance ◽  
Molecular Genetic ◽  
Epidemiologic Study ◽  
Beijing Genotype ◽  
Multiple Drug ◽  
Genetic Characteristics ◽  
Medical Institutions ◽  
Peripheral Lymph ◽  
Biological Risk Factor

The article presents molecular genetic characteristics of M. tuberculosis circulating in Omsk region, as a biologic risk factor of tuberculosis morbidity among medical institutions workers. The authors used descriptive method of epidemiologic study, method of MIRUVNTR-typing of M. tuberculosis DNA. Findings are M. tuberculosis stains of Beijing genotype with VNTR-profile 233325173533424, isolated out of tuberculosis patients cluster being under stationary treatment. 65.2% of the stains had multiple drug-resistance to anti-tuberculosis medications. Respiratory tuberculosis was diagnosed in 85.4% of the cases, extra-pulmonary tuberculosis (urinary system, peripheral lymph nodes, eyes, CNS tuberculosis) equaled 14.6%. Nearly 30% of tuberculosis cases in the health care workers were associated with the bacterioexcretion.

scholarly journals Prevalence of Tuberculosis, Drug Susceptibility Testing, and Genotyping of Mycobacterial Isolates from Pulmonary Tuberculosis Patients in Dessie, Ethiopia

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Minwuyelet Maru ◽  
Solomon H. Mariam ◽  
Tekle Airgecho ◽  
Endalamaw Gadissa ◽  
Abraham Aseffa
Keyword(s):  
Drug Resistance ◽  
Effective Treatment ◽  
Treatment Guidelines ◽  
Multiple Drug Resistance ◽  
Previous Treatment ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Multiple Drug ◽  
Sensitivity Testing ◽  
Cross Sectional

Due to their initially seemingly high cost, timely diagnosis and effective treatment of tuberculosis (TB) are usually hampered by lack or shortage of resources in many high TB burden countries. However, the benefits of effective treatment can eventually outweigh those of empirical treatment. Here, a cross-sectional study was conducted on samples from smear-positive new and retreatment TB patients. Data on sociodemographic and HIV status were collected. Samples were cultured for identification, conventional drug sensitivity testing, and molecular typing by deletion typing and spoligotyping. The results showed the youth were disproportionately affected. New cases were being treated following general treatment guidelines only. Monoresistance or multiple drug resistance was found in 16.5% of new patients. Spoligotyping showed that there were 44 patterns with families H3 and T1 (lineage 4) and CAS-Delhi (lineage 3) being dominant. Some rare patterns from lineage 7 were also found. Spoligotype pattern, HIV positivity, and previous treatment were not associated with drug resistance. That the vast majority of the patients were new cases and young and the large number of these patients with mono- or multiple drug resistance indicate that most TB cases are due to recent transmissions and that urgent actions are needed to curb the transmissions.

scholarly journals Lack of Correlation between embB Mutation and Ethambutol MIC in Mycobacterium tuberculosis Clinical Isolates from China

2007 ◽  
Vol 51 (12) ◽  
pp. 4515-4517 ◽  
Author(s):  
Ruiru Shi ◽  
Jianyuan Zhang ◽  
Koji Otomo ◽  
Guolong Zhang ◽  
Isamu Sugawara
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Susceptibility Testing ◽  
Multiple Drug Resistance ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Clinical Isolates ◽  
Multiple Drug

ABSTRACT Seventy-four Mycobacterium tuberculosis clinical isolates from China were subjected to drug susceptibility testing using ethambutol, isoniazid, rifampin, and ofloxacin. The results revealed that the presence of embB mutations did not correlate with ethambutol resistance but was associated with multiple-drug resistance, especially resistance to both ethambutol and rifampin.

scholarly journals Geographical distribution and antibiotics susceptibility patterns of toxigenic Vibrio cholerae isolates from Kisumu County, Kenya

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Silas O. Awuor ◽  
Eric O. Omwenga ◽  
Ibrahim I. Daud
Keyword(s):  
Drug Resistance ◽  
Vibrio Cholerae ◽  
Multiple Drug Resistance ◽  
Drug Susceptibility ◽  
Multiple Drug ◽  
Biochemical Tests ◽  
Antimicrobial Drug Resistance ◽  
Antibiotics Susceptibility ◽  
Conventional Antibiotics ◽  
Susceptibility Patterns

Background: Multiple drug resistance has become a major threat to the treatment of cholera. Recent studies in Kenya have described the epidemiology, especially the risk factors, of cholera; however, there is little information on the phenotypic and drug susceptibility patterns of Vibrio cholerae (V. cholerae) in outbreaks that in the recent past have occurred in western Kenya.Aim: To characterise and determine the antibiotics’ susceptibility profiling of toxigenic V. cholerae isolates from Kisumu County.Setting: The project was conducted in Kisumu County, Kenya.Methods: A total of 119 V. cholerae O1, biotype El Tor, isolates collected during 2017 cholera outbreak in Kisumu County were used for this study. The samples were cultured on thiosulphate-citrate-bile salts sucrose (TCBS) agar and biochemical tests were carried out using standard procedures. Susceptibility tests were conducted by using various conventional antibiotics against standard procedures.Results: Of the 119 isolates, 101 were confirmed to be V. cholerae belonging to serotypes Inaba and Ogawa, with Inaba being the predominant serotype (73.95%). The isolates were susceptible to ciprofloxacin (100%), ofloxacin (100%), gentamycin (100%), doxycycline (99%), ceftriaxone (99%) and streptomycin (96.04%) antimicrobials, and resistant to erythromycin (53.47%), amoxicillin (64.4%), nalidixic acid (83.2%) and ampicillin (89.11%), with high resistance to cotrimoxazole (99%) and tetracycline (97%).Conclusion: Vibrio cholerae was resistant to multiple antibiotics, including those commonly used in the management of cholera. Taken together, there is a need to carry out regular surveillance on antimicrobial drug resistance during outbreaks.

scholarly journals Clinical and drug resistance characteristics of Providencia stuartii infections in 76 patients

2020 ◽  
Vol 48 (10) ◽  
pp. 030006052096229
Author(s):  
Jiachang Liu ◽  
Ruikai Wang ◽  
Ming Fang
Keyword(s):  
Drug Resistance ◽  
Antimicrobial Agents ◽  
Multiple Drug Resistance ◽  
Drug Susceptibility ◽  
Fourth Generation ◽  
Multiple Drug ◽  
Beta Lactam ◽  
Hospital Department ◽  
Extended Spectrum Beta Lactamase ◽  
Providencia Stuartii

Objectives To investigate the clinical and drug resistance characteristics of Providencia stuartii infections in the Huainan region of Anhui and provide a reference for the clinical selection of antimicrobial agents. Methods This single-center retrospective analysis included 76 patients with P. stuartii infection in Huainan during the period from October 2018 to March 2020. The hospital department in which the patients were treated and the drug susceptibility characteristics of the P. stuartii isolates were recorded. Results Among the 76 patients, the lung was the most common site of infection, and intensive care unit was the main hospital department. Extended spectrum beta-lactamase screening revealed expression by all 76 isolates of P. stuartii. Of the 76 isolates, 92.1% exhibited multiple drug resistance or extensive drug resistance. P. stuartii isolates were sensitive to cefepime and imipenem, but not to other beta-lactam antibiotics. Twenty isolates were resistant to all 21 types of antibiotics. Of the 20 patients infected with extensively drug-resistant isolates, nine (45%) died. Conclusions Drug resistance is increasing in P. stuartii. The antimicrobial agent imipenem may be effective for treatment of P. stuartii infections. Fluoroquinolones, aminoglycosides, and fourth-generation cephalosporins are suitable options for antibiotic therapy.

scholarly journals Selection of Mutations To Detect Multidrug-Resistant Mycobacterium tuberculosis Strains in Shanghai, China

2009 ◽  
Vol 54 (3) ◽  
pp. 1075-1081 ◽  
Author(s):  
Tao Luo ◽  
Ming Zhao ◽  
Xia Li ◽  
Peng Xu ◽  
Xiaohong Gui ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Molecular Genetic ◽  
Drug Susceptibility ◽  
Multidrug Resistant ◽  
Population Based ◽  
Drug Susceptibility Testing ◽  
Population Based Study ◽  
Conventional Culture ◽  
Molecular Genetic Test ◽  
Selection Of

ABSTRACT Novel tools are urgently needed for the rapid, reliable detection of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains of Mycobacterium tuberculosis. To develop such tools, we need information about the frequency and distribution of the mycobacterial mutations and genotypes that are associated with phenotypic drug resistance. In a population-based study, we sequenced specific genes of M. tuberculosis that were associated with resistance to rifampin and isoniazid in 242 phenotypically MDR isolates and 50 phenotypically pan-susceptible isolates from tuberculosis (TB) cases in Shanghai, China. We estimated the sensitivity and specificity of the mutations, using the results of conventional, culture-based phenotypic drug susceptibility testing as the standard. We detected mutations within the 81-bp core region of rpoB in 96.3% of phenotypically MDR isolates. Mutations in two structural genes (katG and inhA) and two regulatory regions (the promoter of mabA-inhA and the intergenic region of oxyR-ahpC) were found in 89.3% of the MDR isolates. In total, 88.0% (213/242 strains) of the phenotypic MDR strains were confirmed by mutations in the sequenced regions. Mutations in embB306 were also considered a marker for MDR and significantly increased the sensitivity of the approach. Based on our findings, an approach that prospectively screens for mutations in 11 sites of the M. tuberculosis genome (rpoB531, rpoB526, rpoB516, rpoB533, and rpoB513, katG315, inhA-15, ahpC-10, ahpC-6, and ahpC-12, and embB306) could detect 86.8% of MDR strains in Shanghai. This study lays the foundation for the development of a rapid, reliable molecular genetic test to detect MDR strains of M. tuberculosis in China.

scholarly journals Tuberculosis outcomes related to the Mycobacterium tuberculosis genotype

2019 ◽  
Vol 9 (3-4) ◽  
pp. 531-538
Author(s):  
O. A. Pasechnik ◽  
A. A. Vyazovaya ◽  
M. A. Dymova ◽  
A. I. Blokh ◽  
V. L. Stasenko ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Drug Susceptibility ◽  
Biological Properties ◽  
Drug Susceptibility Testing ◽  
Beijing Genotype ◽  
Multi Drug Resistance ◽  
Co Morbidity ◽  
Extensive Drug Resistance ◽  
Phylogenetic Lineages

Mycobacterium tuberculosis strains of different phylogenetic lineages and genetic families differ in biological properties that determine, to some extent, epidemiological features and clinical manifestation in tuberculosis (TB) patients.The aim of the study was to assess the risk of an adverse outcome of the disease in TB patients caused by various M. tuberculosis genotypes.Materials and methods. A total of 425 patients with respiratory TB were enrolled in this study. They were registered at phthisiatric facilities in the Omsk region from March 2015 to June 2017 period and included: males — 73.1%, mean age 39.9 years, females — 26.9%, mean age 42.0 years. M. tuberculosis culture and drug susceptibility testing and DNA extraction were performed in accordance with standard methods. Strains were assigned to the M. tuberculosis Beijing genotype and its epidemiologically relevant clusters B0/W148 and 94-32 by PCR based detection of specific markers. Non-Beijing strains were subjected to spoligotyping.Results. We found that 66.5% isolates belonged to the Beijing genotype, 12.8% — to LAM, 10.1% — to T, and 4.7% — to the Ural genotype. Multi-drug resistance (MDR) to anti-TB drugs was observed in 195 M. tuberculosis strains (45.9%). Moreover, Beijing genotype was more often isolated from patients with MDR-TB infection (PR = 2.09 (95% CI 1.6–2.74) and TB infection associated with HIV infection (PR = 1.14 (95% CI 1.01–1.31). Lethal outcome was double higher in patients infected with Beijing vs. non-Beijing strains, 28.6% vs. 14.0% (PR = 2.03; 95% CI 1.3–3.17). The risk factors were identified as follows: young age 18–44 years (RR = 1.7; 95% CI 1.18–2.7), co-morbidity with HIV (RR = 5.0; 95% CI 3.39–7.45), multiple (RR = 1.7; 95% CI 1.14–2.55) and extensive drug resistance (RR = 2.57; 95% CI 1.35–4.92), and association with the Beijing genotype (RR = 2.0, 95% CI 1.3–3.17).Conclusion. M. tuberculosis spread in the Omsk region is characterised by significant prevalence of the Beijing genotype, associated with multiple and extensive drug resistance. A significant association of adverse clinical outcomes and various factors, including association with the Beijing genotype, requires development of new approaches in the fight against tuberculosis.

scholarly journals Self-Reported Risks for Multiple-Drug Resistance among New Tuberculosis Cases: Implications for Drug Susceptibility Screening and Treatment

PLoS ONE ◽  
2011 ◽  
Vol 6 (10) ◽  
pp. e25861 ◽  
Author(s):  
Timothy F. Brewer ◽  
Howard W. Choi ◽  
Carlos Seas ◽  
Fiorella Krapp ◽  
Carlos Zamudio ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Multiple Drug Resistance ◽  
Drug Susceptibility ◽  
Multiple Drug

scholarly journals GENETIC POLYMORPHISM OF TUBERCULOSIS MYCOBACTERIA IN THE CITY WITH LIMITED POPULATION MIGRATION AND HIGH INCIDENCE OF HIV INFECTION

2019 ◽  
Vol 97 (3) ◽  
pp. 40-45
Author(s):  
T. V. Umpeleva ◽  
K. V. Belousova ◽  
L. A. Golubeva ◽  
I. M. Morozova ◽  
N. I. Eremeeva ◽  
...  
Keyword(s):  
Multiple Drug Resistance ◽  
High Rate ◽  
Beijing Genotype ◽  
Multiple Drug ◽  
Newly Diagnosed ◽  
Tuberculosis Patients ◽  
Multiple Drug Resistant ◽  
Previously Treated Patients ◽  
Previously Treated ◽  
The City

87 isolates of M. tuberculosis isolated from tuberculosis patients in the Novouralsk municipality in 2013-2016 were studied. It was found out that a high percentage of isolates had multiple drug resistance: 66 and 85.3% among newly diagnosed and previously treated patients, respectively. Genotyping allowed revealing the dominance of isolates of the Beijing family, both among newly diagnosed (75.5%) and previously treated patients (85.3%), with 52.8 and 67.6% of isolates, respectively, belonging to the Beijing B0/W148 variant. MIRU-VNTR-typing detected nine isolates of the Beijing B0/W148 family with an atypical number of repeats in the QUB26 locus (two). A high rate of tuberculosis with concurrent HIV (39.1%) was noted, while in groups of tuberculosis patients with various HIV statuses, no difference was detected in the ratio of multiple drug resistant pathogens and belonging to the Beijing genotype (BeijingB0/W148). The article demonstrates the possibility of using genotyping technologies to confirm/deny the epidemiological relationship between patients and search for previously unknown mechanisms of tuberculosis infection transmission.

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