scholarly journals ISLET CELL CULTURES AS COMPONENT OF TISSUE-ENGINEERING CONSTRUCT OF PANCREAS

2018 ◽  
Vol 20 (2) ◽  
pp. 74-81
Author(s):  
N. N. Skaletskiy ◽  
G. N. Skaletskaya ◽  
L. A. Kirsanova ◽  
N. V. Baranova ◽  
G. N. Bubentsova ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Progenitor Cells ◽  
Cell Cultures ◽  
Islet Cell ◽  
Morphological Analysis ◽  
Pancreatic Tissue ◽  
Cell Component ◽  
Inverted Microscope ◽  
Tissue Engineering Construct ◽  
Immunohistochemical Methods

Aim:to develop methods for obtaining islet cell cultures for the purpose of their further use as a suitable component of the pancreatic tissue engineered construct. As a source of islet cell cultures, pancreas of newborn rabbits was used as an accessible and well-studied donor model.Materials and methods.For the obtaining of islet cell cultures, pancreas of 1–3-day-old newborn rabbits were used. Changes occurring during the cultivation of pancreatic tissue were recorded using an inverted microscope and a biostation. Morphological analysis of culture samples was carried out using histological and specifi c immunohistochemical methods. The insulin-producing activity of the cultures was determined by enzyme immunoassay.Results.Three main types of cultures were obtained: isletlike organotypic, suspensionalcytotypic, and monolayered, consisting of progenitor cells. Greater morphological safety and adequate insulin-producing ability was revealed in fl oating islet-like cultures.Conclusion.According to their morphofunctional properties, fl otation islet-like cultures obtained from pancreas of the newborn rabbits can be used as basal cell component of the experimental model of the tissue engineered construct of the pancreas. 

Cryopreserved prenatal progenitor cells as cell source for cardiovascular tissue engineering

2007 ◽  
Vol 55 (S 1) ◽  
Author(s):  
D Schmidt ◽  
C Breymann ◽  
J Achermann ◽  
B Odermatt ◽  
M Genoni ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Progenitor Cells ◽  
Cardiovascular Tissue ◽  
Cell Source ◽  
Cardiovascular Tissue Engineering

scholarly journals Binary Biocompatible CNC–Gelatine Hydrogel as 3D Scaffolds Suitable for Cell Culture Adhesion and Growth

Applied Nano ◽  
2021 ◽  
Vol 2 (2) ◽  
pp. 118-127
Author(s):  
Luca Zoia ◽  
Anna Binda ◽  
Laura Cipolla ◽  
Ilaria Rivolta ◽  
Barbara La Ferla
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Cell Cultures ◽  
Cellulose Nanocrystals ◽  
Potential Application ◽  
Chemical Properties ◽  
Cellular Adhesion ◽  
Chemical Crosslinking ◽  
3D Scaffolds ◽  
Physical Chemical

Binary nano-biocomposite 3D scaffolds of cellulose nanocrystals (CNCs)—gelatine were fabricated without using chemical crosslinking additives. Controlled oxidative treatment allowed introducing carboxyl or carbonyl functionalities on the surface of CNCs responsible for the crosslinking of gelatine polymers. The obtained composites were characterized for their physical-chemical properties. Their biocompatibility towards different cell cultures was evaluated through MTT and LDH assays, cellular adhesion and proliferation experiments. Gelatine composites reinforced with carbonyl-modified CNCs showed the most performing swelling/degradation profile and the most promising adhesion and proliferation properties towards cell lines, suggesting their potential application in the field of tissue engineering.

scholarly journals Endometrial SUSD2+ Mesenchymal Stem/Stromal Cells in Tissue Engineering: Advances in Novel Cellular Constructs for Pelvic Organ Prolapse

2021 ◽  
Vol 11 (9) ◽  
pp. 840
Author(s):  
David M. Z. B. Hennes ◽  
Anna Rosamilia ◽  
Jerome A. Werkmeister ◽  
Caroline E. Gargett ◽  
Shayanti Mukherjee
Keyword(s):  
Tissue Engineering ◽  
Pelvic Organ Prolapse ◽  
Progenitor Cells ◽  
Cellular Therapy ◽  
Personalised Medicine ◽  
Pelvic Organ ◽  
Vaginal Tissue ◽  
Tissue Integration ◽  
Preclinical Trials ◽  
And Control

Cellular therapy is an emerging field in clinical and personalised medicine. Many adult mesenchymal stem/progenitor cells (MSC) or pluripotent derivatives are being assessed simultaneously in preclinical trials for their potential treatment applications in chronic and degenerative human diseases. Endometrial mesenchymal stem/progenitor cells (eMSC) have been identified as clonogenic cells that exist in unique perivascular niches within the uterine endometrium. Compared with MSC isolated from other tissue sources, such as bone marrow and adipose tissue, eMSC can be extracted through less invasive methods of tissue sampling, and they exhibit improvements in potency, proliferative capacity, and control of culture-induced differentiation. In this review, we summarize the potential cell therapy and tissue engineering applications of eMSC in pelvic organ prolapse (POP), emphasising their ability to exert angiogenic and strong immunomodulatory responses that improve tissue integration of novel surgical constructs for POP and promote vaginal tissue healing.

Abstract P034: Bone Marrow--Derived Cells Are Not an Essential Component in Cardiosphere Formation

2011 ◽  
Vol 109 (suppl_1) ◽  
Author(s):  
Jianqin Ye ◽  
Andrew Boyle ◽  
Yerem Yeghiazarians
Keyword(s):  
Myocardial Infarction ◽  
Bone Marrow ◽  
Progenitor Cells ◽  
Small Cells ◽  
Immunomagnetic Beads ◽  
Cell Component ◽  
Post Surgery ◽  
C57bl Mice ◽  
Bone Marrow Derived Cells

Background: Cardiospheres (CS) are composed of heterogeneous population of cells but it is unknown whether bone marrow derived cells are an essential cell component in CS formation. Methods: Chimera mice were generated by transplantation of bone morrow cells from GFP transgenic mice to irradiated C57BL mice. Mice were randomized into 3 groups 5 months after transplantation: 1) myocardial infarction; 2) sham operated; 3) un-operated (n=5/group). Hearts were harvested 2-weeks post-surgery. Cardiac explants were cultured and putative cardiosphere forming cells (CFCs) (small cells migrating out from the explants) were collected 14 days later and reseeded on new culture dishes for CS formation. The number of CS from each heart was counted at 3 days. CS cell composition was analyzed by FACS. To further analyze the role of bone marrow derived CD45+ cells in forming CS, CD45+ cells was isolated from CFCs by CD45 antibody coated immunomagnetic beads. The number of CS formed from 1×10 5 putative CFCs, CFCs without CD45+ cells and CD45+ cells from CFCs (n=6-9/cell type) respectively were also counted at 3 days in culture. Results: Compared to sham (122± 23/heart) and un-operated hearts (18± 5/heart), infarcted hearts formed more CS (357± 64/heart, P<0.01). In all groups, irrespective of any surgery, 18.4± 4.5% of cells in CS co-expressed GFP and CD45, indicating they originated in bone marrow. Low percentage of bone marrow stem/progenitor cells (3.9% Sca-1+GFP+CD45+ and 1% c-Kit+GFP+CD45+ cells) were detected in CS, but a high percentage of cells within CS were cardiac stem/progenitor cells (26.3± 9.4% cells were Sca-1+GFP-CD45-, 0.10± 0.04% c-Kit+GFP-CD45-). Depleting CD45+ cells from putative CFCs actually increased the formation of CS (67±10 CS/1×10 5 cells) compared to un-depleted CFCs (51± 6 CS/1×10 5 cells, P<0.0001). Purified CD45+ cells from CFCs did not form CS in culture. Conclusion: Myocardial infarction increases the formation of CS in culture. Bone marrow derived CD45+ cells make up a small percentage of CS, but are not necessary for CS formation.

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