scholarly journals RAPD Marker Conversion into a SCAR Marker for Rapid Identification of Johnsongrass [Sorghum halepense (L.) Pers.]

2013 ◽  
Vol 41 (1) ◽  
pp. 306 ◽  
Author(s):  
Wenju ZHANG ◽  
Shasha WEI ◽  
Liping YIN ◽  
Zhirui DENG ◽  
Jianping YI ◽  
...  
Keyword(s):  
Scar Marker ◽  
Rapd Marker ◽  
Morphological Characters ◽  
Rapid Identification ◽  
Sorghum Halepense ◽  
Germplasm Identification ◽  
Marker Conversion ◽  
The World ◽  
Sequence Characterized Amplified Regions ◽  
Scar Primers

Johnsongrass [Sorghum halepense (L.) Pers.] is a malignant weed in the world, threatening biodiversity at invaded habitats in more than 50 countries. Because of similarity in morphological characters, S. halepense and its relatives, S. almum, S. nitidum, S. propinquum, S. sudanense, and S. bicolor, etc. was difficult to identify. As a supplementary methodolgy for morphology identification, a molecular detection method was established. Sequence Characterized Amplified Regions (SCAR) marker is a recent established, reliabile, and stable molecular marker based on RAPD maker, an effective way for germplasm identification. In this study, one specific band of S.halepense was screened by 163 pairs of RAPD primers. According to the sequences of the band, a pair of special SCAR primers SH1/SH2 was designed and verified by 65 Sorghum DNA samples from all over the world. The results showed SCAR primers SH1/SH2 can be used to distinguish S.halepense and its relatives rapidly with three exceptions of Australia geotypes.

Distribution of proazulenes in Achillea millefolium s.l. wild populations in relation to phytosociological dependence and morphological characters

2005 ◽  
Vol 3 (2) ◽  
pp. 136-143 ◽  
Author(s):  
Jolita Radušiene ◽  
Odeta Gudaityte
Keyword(s):  
Plant Biomass ◽  
Hierarchical Cluster ◽  
Morphological Characters ◽  
Rapid Identification ◽  
Raw Material ◽  
Achillea Millefolium ◽  
Wild Populations ◽  
Multivariate Statistical ◽  
Morphological Marker ◽  
Node Number

Millefolii herba is one of the oldest and most important drugs in Lithuania, used both in folk and official medicine. The raw material is usually gathered from spontaneous populations. It would be reasonable to develop methods to identify the populations which produce high plant biomass and accumulate high levels of proazulenes. The quantitative variation of proazulenes and their relationships with phytosociological dependence and morphological characters of Achillea millefolium from wild populations has been examined. The vegetation records were made at 140 growing sites of A. millefolium. The species was found in 29 types of community representing different growing habitats. The resources of milfoils prevailed in dry grasslands of Arrhenatheretalia communities and ruderal habitats of Dauco-Meliliotion. The data on productivity and morphological characters were subjected to multivariate statistical analysis. A hierarchical cluster analysis indicated that higher productivity of A. millefolium populations was associated with their dependence on ruderal and segetal communities. The testing of A. millefolium plants revealed high variation for proazulenes in different communities. The proazulene-containing plants have been identified in 38% of all analysed cases. In the highest segetal and wasteland communities of Agropyretalia repentis, Sisybietalia, Digitario-Setarion, Aperion spicae-venti, Senecion fluviatilis only proazulene-free plants were found. Results of the ANOVA showed the proazulene-containing and proazulene-free plants significantly differed (P<0.05) for width of leaves and number of nodes. The presence of nodes in high number and narrow leaves in high proazulene-containing plants distinctly marked them from the proazulene-free plants. The study raised the possibility that selection based on the two morphological marker traits (node number and leaf width) and proazulene distribution in the populations would be effective for rapid identification of highly productive population(s) of pharmaceutical importance.

Machine Learning Arrives in Archaeology

2021 ◽  
Vol 9 (2) ◽  
pp. 186-191
Author(s):  
Simon H. Bickler
Keyword(s):  
Machine Learning ◽  
Rapid Development ◽  
Rapid Identification ◽  
First Century ◽  
Traditional Methods ◽  
Archaeological Practice ◽  
The World ◽  
Archaeological Work ◽  
Archaeological Analysis

OverviewMachine learning (ML) is rapidly being adopted by archaeologists interested in analyzing a range of geospatial, material cultural, textual, natural, and artistic data. The algorithms are particularly suited toward rapid identification and classification of archaeological features and objects. The results of these new studies include identification of many new sites around the world and improved classification of large archaeological datasets. ML fits well with more traditional methods used in archaeological analysis, and it remains subject to both the benefits and difficulties of those approaches. Small datasets associated with archaeological work make ML vulnerable to hidden complexity, systemic bias, and high validation costs if not managed appropriately. ML's scalability, flexibility, and rapid development, however, make it an essential part of twenty-first-century archaeological practice. This review briefly describes what ML is, how it is being used in archaeology today, and where it might be used in the future for archaeological purposes.

A cladistic analysis of the Old World species of Lotus L. (Fabaceae: Loteae)

2000 ◽  
Vol 78 (3) ◽  
pp. 351-360 ◽  
Author(s):  
Ana M Arambarri
Keyword(s):  
Cladistic Analysis ◽  
Morphological Characters ◽  
Comparison Method ◽  
Data Matrix ◽  
Consensus Tree ◽  
Strict Consensus Tree ◽  
World Species ◽  
Old World ◽  
Species Phylogeny ◽  
The World

The diagnostic characters of the genus Lotus L. are a claw with a thickened infolded margin, diadelphous stamens, and a style hardened from the base. This genus contains about 100 species that are distributed throughout the world. To investigate the phylogeny of the Old World species of Lotus, subgenus Edentolotus, sections Krokeria, Xantholotus, and Erythrolotus, a cladistic analysis was performed using 31 morphological characters. To test the phylogenetic relationships among species of Lotus-Edentolotus and Dorycnium, Pedrosia, and Tetragonolobus, these taxa were included as part of the ingroup. The polarity of the characters was based on the outgroup comparison method, using Anthyllis as one outgroup and Tripodion as another. The analysis with Anthyllis as outgroup yielded eight equally parsimonious trees (with all characters equally weighted), each with 62 steps, a consistency index of 0.53, and a retention index of 0.75. All trees (including the strict consensus tree from the eight initial trees) showed that genus Lotus, subgenus Edentolotus, and sections Xantholotus and Erythrolotus are polyphyletic, with only section Krokeria appearing as monophyletic. On the other hand, the groups of species Lotus angustissimus, Lotus corniculatus, Lotus creticus, and Lotus peregrinus are monophyletic. Identical results were derived from the data matrix using Tripodion as the outgroup. Results are compared with previous cytogenetic and biochemical evidence.Key words: cladistic analysis, Fabaceae, Loteae, Lotus, Old World species, phylogeny.

scholarly journals Taxonomy of the genus Commelina Plum. ex L. (Commelinaceae) in Nepal

2010 ◽  
Vol 6 ◽  
pp. 25-31 ◽  
Author(s):  
Jyoti P Gajurel ◽  
Krishna K Shrestha
Keyword(s):  
Key Words ◽  
Taxonomic Status ◽  
Morphological Characters ◽  
Species Level ◽  
Morphological Data ◽  
Plant Science ◽  
The World ◽  
Voucher Specimens ◽  
Key Words Anatomy ◽  
Anatomical Characters

About 170 species of Commelina are known from tropical and subtropical regions of the world. Nepal contributes 6 species of Commelina to the world flora. In the present work, taxonomy of all the six species of Commelina reported from Nepal (C. benghalensis, C. caroliniana, C. diffusa, C. maculata, C. paludosa and C. suffruticosa) was studied. Voucher specimens were collected from Central and Eastern Nepal, covering 14 districts. Morphological characters were studied from these collections. Palynological and anatomical characters were also used to see if they are taxonomically important to delimit the taxa within Commelina. Morphological characters seemed promising to delimit the taxa within Commelina. The key identifying characters at species level are modification in root, form of spathe, structure of leaves and seeds, shape of stomata and pollen. Palynological and anatomical characters were also useful, to some extent, in separating some species, but were not significant as compared to morphological data. Some specimens, close to C. benghalensis and C. caroliniana, showed very different characters. Thus further study is needed to confirm their taxonomic status. Key-words: anatomy; flora; morphological characters; palynology; voucher specimens.DOI: 10.3126/botor.v6i0.2907 Botanica Orientalis - Journal of Plant Science (2009) 6: 25-31

scholarly journals GeneXpert MTB/RIF Assay for Rapid Identification of Mycobacterium Tuberculosis and Rifampicin Resistance Directly from Sputum Sample

2017 ◽  
Vol 7 (2) ◽  
pp. 86-89 ◽  
Author(s):  
Nourjahan Laskar ◽  
Md Akram Hossain ◽  
Jannatul Fardows ◽  
Mominur Rahman
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Sputum Sample ◽  
Rapid Identification ◽  
Rapid Diagnosis ◽  
World Health ◽  
Multi Drug Resistance ◽  
Drug Resistant ◽  
Resistant Tuberculosis ◽  
The World ◽  
Health Organization

Background: The World Health Organization has endorsed the use of molecular methods for the detection of tuberculosis (TB) and drug resistant TB as a rapid method. In Bangladesh, the Xpert MTB/RIF assay has been implemented into reference laboratories for diagnosis of TB and also MDR TB.Objective: Drug resistant tuberculosis has long been a common problem prevailing in our country. The present study focused on the rapid identification of Mycobacterium tuberculosis as well as drug resistance.Materials and Methods: Sputum samples from a total of 107 cases, assumed as multi-drug resistance tuberculosis, were studied through GeneXpert assay.Results: Out of 107 cases, 91 (85.05%) were detected having M. tuberculosis ? 64 (59.81%) were rifampicin sensitive and 27 (25.23%) were rifampicin resistant. The sensitivity and specificity of the GeneXpert are 87.64% and 75% respectively.Conclusion: GeneXpert assay can be considered for the rapid diagnosis of drug resistant tuberculosis.J Enam Med Col 2017; 7(2): 86-89

Six new species of the genus Chevalia Walker, 1904 (Amphipoda, Corophiidea, Chevaliidae) from Brazilian waters, with a key to world species of the genus

Zootaxa ◽  
2019 ◽  
Vol 2713 (1) ◽  
pp. 25
Author(s):  
JESSER F. SOUZA-FILHO ◽  
ANA MARIA T. SOUZA ◽  
MARIA TERESA VALÉRIO-BERARDO
Keyword(s):  
New Species ◽  
Morphological Characters ◽  
Species Group ◽  
Brazilian Coast ◽  
Proximal Margin ◽  
World Species ◽  
Group Iii ◽  
Cephalic Lobe ◽  
Group I ◽  
The World

Six new species of the genus Chevalia are described from the Brazilian coast using all morphological characters proposed by Barnard & Thomas (1987) and Lazo-Wazen (1999) with two more: the shape of head proximal margin of lateral cephalic lobe and length ratio of uropod 2 rami: C. anomala sp. nov.; C. caetes sp. nov.; C. convexa sp. nov.; C. marajoara sp. nov.; C. thomasi sp. nov.; and C. setosa sp. nov. This paper raises the total number of recognized world species in this genus to 13. The genus is herein subdivided into three groups, based only on the shape of the basis of pereopod 7: group I – rectangular basis of pereopod 7 with a protuberant posteroventral corner, comprises four species; group II – rectangular basis of pereopod 7 lacking a protuberant posteroventral corner, comprises three species; and group III – ovate basis of pereopod 7, comprises seven species. A key of the world Chevalia species is also provided.

Description of Ditylenchus paraparvus n. sp. from Iran with an updated list of Ditylenchus Filipjev, 1936 (Nematoda: Anguinidae)

Zootaxa ◽  
2019 ◽  
Vol 4651 (1) ◽  
pp. 85-113
Author(s):  
KOBRA HASHEMI ◽  
AKBAR KAREGAR
Keyword(s):  
New Species ◽  
Morphological Characters ◽  
Valid Species ◽  
Morphometric Data ◽  
Body Width ◽  
Pharyngeal Bulb ◽  
Lateral Field ◽  
The World ◽  
Short Body ◽  
One New Species

During a survey, 12 known and one new species of Ditylenchus Filipjev, 1936 were collected from southern provinces of Iran during 2013–2017. Ditylenchus paraparvus n. sp. is characterised by a short body length (441–543 µm), lateral field with four lines, delicate and short stylet (6–7.5 µm) with small rounded or posteriorly sloping knobs, pyriform and offset basal pharyngeal bulb, V = 67.7–76.4, short post-vulval uterine sac less than one vulval body width long, small spicules (12.5–15 µm) and almost cylindrical tail (71–90 µm) with rounded end. Morphometric data of studied species are presented and intraspecific variation of their morphometrics and morphological characters is discussed. The list of the world Ditylenchus species is updated, and a dichotomous identification key and an updated tabular compendium for 63 valid species is provided. 

Phylogenetic relationships and classification of the Chloropinae of the world (Diptera: Chloropidae)

2020 ◽  
Vol 190 (3) ◽  
pp. 889-941
Author(s):  
Paula Raile Riccardi ◽  
Dalton De Souza Amorim
Keyword(s):  
Phylogenetic Relationships ◽  
Morphological Traits ◽  
Analytical Approach ◽  
Morphological Characters ◽  
Phylogenetic Study ◽  
New Genera ◽  
Compelling Evidence ◽  
Incertae Sedis ◽  
The World

Abstract The Chloropidae is a species-rich family of flies with about 3000 species in four subfamilies. The Chloropinae is the second most species-rich subfamily with almost 1000 described species in 75 accepted genera. There is agreement about the monophyly of the subfamily; however, the relationships among the genera are still poorly understood and some genera are clearly paraphyletic. Thus, the interpretation of the evolution of morphological traits, such as male terminalia sclerites, remains challenging. This is the first phylogenetic study of the Chloropinae using a formal analytical approach, including representatives of 73 genera of the subfamily and 124 morphological characters. The monophyly of the Chloropinae is corroborated. Chloropella is sister to the remainder of the subfamily. Slightly different analytical procedures show stable clades and rogue taxa. We propose a system for the subfamily with ten tribes, three of which are newly proposed here—Chloropellini trib. nov., Chloropini, Chloropsinini trib. nov., Diplotoxini, Eurinini stat. nov., Lasiosinini, Mepachymerini, Meromyzini, Mindini and Pseudothaumatomyini. Eight genera are kept incertae sedis and two new genera are erected. There is compelling evidence that Chlorops and Ectecephalina are paraphyletic.

Two new species of nereidids (Annelida, Polychaeta) from Taiwan

Zootaxa ◽  
2019 ◽  
Vol 4652 (3) ◽  
pp. 544-556
Author(s):  
PAN-WEN HSUEH
Keyword(s):  
New Species ◽  
Southeast Asia ◽  
Morphological Characters ◽  
Fouling Community ◽  
The World ◽  
Two New Species ◽  
Aquaculture Ponds ◽  
East And Southeast Asia ◽  
Southern Taiwan

Two new species of nereidids, Dendronereis chipolini n. sp. and Neanthes hsinchuensis n. sp., collected from brackish aquaculture ponds near coasts of southern Taiwan and fouling community on docks of the Hsinchu fishing port in northwestern Taiwan, respectively, are described in the present study. Dendronereis chipolini n. sp. differs from its congeners by a combination of number and morphology of branchiae, morphology of neuropodia and form of neuropodial homogomph spinigers. Neanthes hsinchuensis n. sp. can be distinguished from congeners reported from East and Southeast Asia by a combination of numbers of conical paragnaths, morphology of notopodia, the absent/present of prechaetal lobe and forms of neuropodial chaetae. A key to Dendronereis species of the world is included, together with a table of morphological characters of Neanthes species reported from East and Southeast Asia, which have no conical paragnaths on Area V of the pharynx. 

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