Ampelographic and Genetic Characterization of Grapevine Varieties (Vitis vinifera L.) of the ‘Mavroudia’ Group Cultivated in Greece

Twenty-one grapevine varieties grown all over Greece and belonging to ‘Mavroudia’ group were ampelographically described and genotyped by AFLP molecular analysis in order to discriminate the varieties, synonyms, homonyms and variations of the group. In most cases, the molecular findings confirmed the results of the ampelographic description. In general, and despite the high degree of genetic similarity between certain pairs of the studied cultivars, the group of ‘Mavroudia’ was characterized as being heterogeneous. From the studied cultivars, ‘Kountoura mavri’, ‘Mavro Spetson’ and ‘Pappoudes’ showed very high degree of genetic similarity, sustaining the hypothesis that the last two are clones of the first. Grapevine cultivar ‘Pappoudes’ was for the first time ampelographically described and identified as being closely related to ‘Kountoura mavri’. High degree of genetic similarity was observed between cultivars ‘Gaidouricha’ and ‘Agiomavritiko’, suggesting that they probably originated from the same parent variety through the accumulation of mutations. This may also be true for cultivars ‘Mavrokorakas’ and ‘Kartsiotis’. Also, the results from the statistical analysis showed that ‘Mavro Arachovis’, ‘Mavroudi Voulgarias’ and ‘Voulgaroudes’, despite the relatively high genetic similarity between them, are different. The same applies for the rest of the cultivars studied, while ‘Mavro Kalavriton’, the most widespread variety of the ‘Mavroudia’ group, showed the lowest degree of genetic similarity within the all the cultivars studied. The ampelographic description in combination with the molecular method AFLP are effective for the study of the between and within genetic diversity of grapevine cultivars as well as for their identification and discrimination.

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Genetic Study of Native Grapevine Varieties of Northern, Western and Central Greece with the Use of Ampelographic and Molecular Methods

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha47111213 ◽

2018 ◽

Vol 47 (1) ◽

pp. 46-53 ◽

Cited By ~ 1

Author(s):

Katerina BINIARI ◽

Maritina STAVRAKAKI

Keyword(s):

International Organization ◽

Vitis Vinifera L ◽

Molecular Method ◽

Northern Greece ◽

Central Greece ◽

Grape Berries ◽

Previous Hypothesis ◽

Parent Variety ◽

Place Of Origin ◽

High Degree

The aim of this study was the identification and discrimination of 49 grapevine varieties that are cultivated in northern, western and central Greece with the use of the ampelographic description and the molecular method RAPD. The grapevine varieties were located in their cultivation centers and the studied samples were collected from productive vineyards of these regions. For the ampelographic description, 22 ampelographic characters were used following a list of descriptors developed by the International Organization of Vine and Wine (OIV), while for the molecular analysis 8 of the most polymorphic primers were used. The results showed that: (a) there is high degree of genetic heterogeneity among most of the varieties studied, (b) grapevine varieties ‘Xinomavro’ and ‘Zalovitiko’ exhibited identity with both methods used, therefore the latter constitutes a synonym/clone of the former, (c) high degree of genetic similarity was recorded between cv ‘Stavroto’ and ‘Abelakiotiko’, a result enhancing the view that they constitute biotypes/clones of an original/parent variety and originated through the accumulation of mutations, (d) a previous hypothesis is confirmed. This hypothesis states that in the vineyards of northern Greece, different varieties of Vitis vinifera L. as well as hybrids (direct producers) were imported. Names/synonyms were given to these imported varieties and hybrids related to their place of origin or the morphological traits of the grape/berries implying identity among them (‘Mavroudi’, ‘Voulgariko’, ‘Voulgaroudia’, ‘Vapsa’ etc.), while they are different varieties, (e) the combination of the ampelographic description and the molecular method RAPD is very effective in the identification and discrimination of grapevine cultivars.

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TBE in Sweden

Tick-borne encephalitis - The Book ◽

10.33442/26613980_12b32-3 ◽

2020 ◽

Keyword(s):

Genetic Characterization ◽

Immunoglobulin M ◽

Enzyme Linked Immunosorbent Assay ◽

Second Phase ◽

Serum Samples ◽

Tick Borne Encephalitis ◽

Specific Immunoglobulin ◽

Tick Borne Encephalitis Virus ◽

First Time

Tick-borne encephalitis virus (TBEV) was isolated for the first time in Sweden in 1958 (from ticks and from 1 tick-borne encephalitis [TBE] patient).1 In 2003, Haglund and colleagues reported the isolation and antigenic and genetic characterization of 14 TBEV strains from Swedish patients (samples collected 1991–1994).2 The first serum sample, from which TBEV was isolated, was obtained 2–10 days after onset of disease and found to be negative for anti-TBEV immunoglobulin M (IgM) by enzyme-linked immunosorbent assay (ELISA), whereas TBEV-specific IgM (and TBEV-specific immunoglobulin G/cerebrospinal fluid [IgG/CSF] activity) was demonstrated in later serum samples taken during the second phase of the disease.

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Characterization of SrBi2Nb2O9 Thin Films Prepared by Sol Gel Technique

MRS Proceedings ◽

10.1557/proc-459-213 ◽

1996 ◽

Vol 459 ◽

Cited By ~ 7

Author(s):

E. Ching-Prado ◽

W. Pérez ◽

A. Reynés-Figueroa ◽

R. S. Katiyar ◽

D. Ravichandran ◽

...

Keyword(s):

Thin Films ◽

Raman Band ◽

Sol Gel ◽

Orthorhombic Symmetry ◽

X Ray Diffraction ◽

Ft Ir ◽

Gel Technique ◽

High Degree ◽

First Time

ABSTRACTThin films of SrBi2Nb2O9 (SBN) with thicknesses of 0.1, 0.2, and 0.4 μ were grown by Sol-gel technique on silicon, and annealed at 650°C. The SBN films were investigated by Raman scatering for the first time. Raman spectra in some of the samples present bands around 60, 167, 196, 222, 302, 451, 560, 771, 837, and 863 cm−1, which correspond to the SBN formation. The study indicates that the films are inhomogeneous, and only in samples with thicknesses 0.4 μ the SBN material was found in some places. The prominent Raman band around 870 cm−1, which is the A1g mode of the orthorhombic symmetry, is assigned to the symmetric stretching of the NbO6 octahedrals. The frequency of this band is found to shift in different places in the same sample, as well as from sample to sample. The frequency shifts and the width of the Raman bands are discussed in term of ions in non-equilibrium positions. FT-IR spectra reveal a sharp peak at 1260 cm−1, and two broad bands around 995 and 772 cm−1. The bandwidths of the latter two bands are believed to be associated with the presence of a high degree of defects in the films. The experimental results of the SBN films are compared with those obtained in SBT (T=Ta) films. X-ray diffraction and SEM techniques are also used for the structural characterization.

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Natural Infection of Southern Highbush Blueberry (Vaccinium corymbosum Interspecific Hybrids) by Xylella fastidiosa subsp. fastidiosa

Plant Disease ◽

10.1094/pdis-11-19-2477-re ◽

2020 ◽

Vol 104 (10) ◽

pp. 2598-2605

Author(s):

Dario Di Genova ◽

Kippy J. Lewis ◽

Jonathan E. Oliver

Keyword(s):

Genetic Characterization ◽

Xylella Fastidiosa ◽

Natural Infection ◽

Vaccinium Corymbosum ◽

Significant Similarity ◽

Potential Impact ◽

Southern Highbush ◽

Southern Georgia ◽

First Time

Xylella fastidiosa (Xf) is an emerging insect-vectored, xylem-limited bacterium that can cause disease on several economically important fruit and tree crops including almond, blueberry, citrus, grapevine, peach, and pecan. On blueberry, Xf causes bacterial leaf scorch (BLS), which is prevalent in the southeastern United States. This disease, previously reported to be caused by Xf subsp. multiplex (Xfm), can result in rapid plant decline and death of southern highbush (SHB) blueberry cultivars. In 2017, a survey of blueberry plantings in southern Georgia (U.S.A.) confirmed the presence of Xf-infected plants in eight of nine sites examined, and seven isolates were cultured from infected plants. Genetic characterization of these isolates through single-locus and multilocus sequence analysis revealed that three isolates from two sites belonged to Xf subsp. fastidiosa (Xff), with significant similarity to isolates from grapevine. After these three isolates were artificially inoculated onto greenhouse-grown SHB blueberries (cv. ‘Rebel’), symptoms typical of BLS developed, and Xff infection was confirmed through genetic characterization and reisolation of the bacterium to fulfill Koch’s postulates. Because all previously reported Xf isolates from blueberry have been characterized as Xfm, this is the first time that isolation of Xff has been reported from naturally infected blueberry plantings. The potential impact of Xff isolates on disease management in blueberry requires further exploration. Furthermore, given that isolates from both Xfm and Xff were obtained within a single naturally infected blueberry planting, blueberry in southern Georgia may provide opportunities for intersubspecific recombination between Xff and Xfm isolates.

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Genetic Characterization of Cleveland Bay Horse Breed

Diversity ◽

10.3390/d11100174 ◽

2019 ◽

Vol 11 (10) ◽

pp. 174 ◽

Cited By ~ 2

Author(s):

Khanshour ◽

Hempsey ◽

Juras ◽

Cothran

Keyword(s):

Genetic Diversity ◽

Genetic Variability ◽

Genetic Similarity ◽

Genetic Resource ◽

Genetic Characterization ◽

Genetic Relationships ◽

Similarity Measures ◽

Horse Breed ◽

The Usa

The Cleveland Bay (CB) is the United Kingdom’s oldest established horse breed. In this study we analyzed the genetic variability in CB horses and investigated its genetic relationships with other horse breeds. We examined the genetic variability among 90 CB horses sampled in the USA compared to a total of 3447 horses from 59 other breeds. Analysis of the genetic diversity and population structure was carried out using 15 microsatellite loci. We found that genetic diversity in CB horses was less than that for the majority of other tested breeds. The genetic similarity measures showed no direct relationship between the CB and Thoroughbred but suggested the Turkman horses (likely in the lineage of ancestors of the Thoroughbred) as a possible ancestor. Our findings reveal the genetic uniqueness of the CB breed and indicate its need to be preserved as a genetic resource.

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Nanosize CaCu3Ti4O12 Green Synthesis and Characterization of a Precursor Oxalate Obtained from Averrhoa carambola Fruit Juice and Its Thermal Decomposition to the Perovskite

Journal of Nanomaterials ◽

10.1155/2020/8830136 ◽

2020 ◽

Vol 2020 ◽

pp. 1-7

Author(s):

Ekane Peter Etape ◽

Josepha Foba-Tendo ◽

Beckley Victorine Namondo ◽

Divine Mbom Yufanyi ◽

Hypolite Mathias Kamta Tedjieukeng ◽

...

Keyword(s):

Fruit Juice ◽

Thermal Treatments ◽

Decomposition Products ◽

Multiple Dimensions ◽

Different Temperatures ◽

Synthesis Routes ◽

High Degree ◽

Very High ◽

Oxalic Acids

Improving on the very high temperatures used in solid-state synthesis routes to prepare CCTO ignited the idea of using oxalate routes which make use of organic solvents in the synthesis of CCTOX as oxalate intermediates to the decomposition product, CCTO. The use of commercial oxalic acids and oxalate has not only recorded a solubility problem which reflects on the size, shape, homogeneity, and morphology of the final product but also has an environmental impact originating from the solvents used. Both the composition and morphology of these inhomogeneities play a role in the behaviour of the final product, pointing out the need to assess the dependence of size, shape, homogeneity, and morphology and the material performance on the sample synthesis history. In this study, nanosized particles of calcium copper titanium oxide, CaCu3Ti4O12 (CCTO), were successfully synthesized by pyrolysis of the corresponding heterometal oxalate precursors obtained via coprecipitation using the edible carambola fruit juice as a precipitating agent and investigated in detail. The precursors were characterized, and the results revealed the formation of a single molecular precursor represented by the formula CaCu3(TiO)4(C2O4)8·9H2O (CCTOX). The decomposition products, obtained via calcination in air, were subsequently subjected to thermal treatments at different temperatures for 4 hours. The morphology and microstructure were characterized, and analysis showed the formation of a single phase, CaCu3Ti4O12 (CCTO) with CuO and CaTiO3 as impurity. It was observed from microscopy that the samples obtained from sintering at 600°C for four hours had discrete particles with regular morphology, limited size distribution, high degree of homogeneity, and multiple dimensions ranging between 10 and 35 nm and showed some degree of ellipticity in shape. Increasing the sintering temperature from 600°C to 700°C and 800°C increased the grain growth in the ceramic as well as the densification. The method makes advantage of the fact that oxalate precursors decomposed at relatively lower temperatures and the fact that the oxalate in the juice is in the solution which downplays both the solubility and environmental pollution problems since no additional solvents are used.

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Molecular characterization of begomoviruses and DNA satellites from Vietnam: additional evidence that the New World geminiviruses were present in the Old World prior to continental separation

Journal of General Virology ◽

10.1099/vir.0.83236-0 ◽

2008 ◽

Vol 89 (1) ◽

pp. 312-326 ◽

Cited By ~ 89

Author(s):

Cuong Ha ◽

Steven Coombs ◽

Peter Revill ◽

Rob Harding ◽

Man Vu ◽

...

Keyword(s):

New World ◽

Bipartite Begomoviruses ◽

Bipartite Begomovirus ◽

Stem Loop ◽

Old World ◽

Recombinant Viruses ◽

Virus Diversity ◽

High Degree ◽

First Time

Sixteen viruses, belonging to 16 species of begomovirus, that infect crops and weeds in Vietnam were identified. Sequence analysis of the complete genomes showed that nine of the viruses (six monopartite and three bipartite) belong to novel species and five of them were identified in Vietnam for the first time. Additionally, eight DNA-β and three nanovirus-like DNA-1 molecules were also found associated with some of the monopartite viruses. Five of the DNA-β molecules were novel. Importantly, a second bipartite begomovirus, Corchorus golden mosaic virus, shared several features with the previously characterized virus Corchorus yellow vein virus and with other bipartite begomoviruses from the New World, supporting the hypothesis that New World-like viruses were present in the Old World. This, together with a high degree of virus diversity that included putative recombinant viruses, satellite molecules and viruses with previously undescribed variability in the putative stem–loop sequences, suggested that South-East Asia, and Vietnam in particular, is one of the origins of begomovirus diversity.

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Characterization of Spanish grapevine cultivar diversity using sequence-tagged microsatellite site markers

Genome ◽

10.1139/g02-098 ◽

2003 ◽

Vol 46 (1) ◽

pp. 10-18 ◽

Cited By ~ 66

Author(s):

J P Martín ◽

J Borrego ◽

F Cabello ◽

J M Ortiz

Keyword(s):

Plant Material ◽

Vitis Vinifera L ◽

Germplasm Bank ◽

Discrimination Power ◽

Grapevine Cultivar ◽

Genotype Identification ◽

Site Markers ◽

Expected Values ◽

Cultivar Diversity

A broad germplasm bank collection containing most of the autochthonous Spanish grapevine cultivars was analyzed using six sequence-tagged microsatellite site (STMS) loci: VVS2, VVMD5, VVMD7, ssrVrZAG47, ssrVrZAG62, and ssrVrZAG79. The number of alleles obtained ranged from 9 in ssrVrZAG47 to 13 in VVS2, and the observed genotypes per locus varied between 24 (ssrVrZAG47) and 41 (VVSS2). A total of 57 unique genotypes were obtained considering all 6 loci, and 40 varieties presented at least 1 of these specific genotypes. The genotypic combinations for the 6 loci have generated 163 different profiles in the 176 cultivars. Ten pairs of accessions and one group of four Garnacha's cultivars can not be differentiated. The observed heterozygosity varied between 75.6 (VVMD7) and 90.9% (VVMD5), without significant differences from the expected values for any loci. The VVMD5 locus was the most informative, and also showed the highest discrimination power. The cumulative discrimination power for all six loci was practically 1; however, in fact, these STMS loci have differentiated only about 93% of the accessions, probably owing to high relatedness of the plant material. Usefulness of this STMS set for characterization of a Spanish grapevine collection is emphasized, as well as the elaboration of databases with these molecular markers.Key words: Vitis vinifera L., STMS markers, genotype identification, grapevine synonymies, germplasm bank.

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GENOTYPE-ENVIRONMENT INTERACTIONS AND INHERITANCE OF QUANTITATIVE TRAITS IN A HYBRID COMBINATION BETWEEN SEEDED AND SEEDLESS GRAPEVINE CULTIVARS (VITIS VINIFERA L.)

PROCEEDINGS ON APPLIED BOTANY GENETICS AND BREEDING ◽

10.30901/2227-8834-2019-4-99-112 ◽

2020 ◽

Vol 180 (4) ◽

pp. 99-112

Author(s):

V. Roychev

Keyword(s):

Quantitative Traits ◽

Dominant Gene ◽

Vitis Vinifera L ◽

Hybrid Combination ◽

Grapevine Cultivar ◽

Hybrid Plants ◽

Very High ◽

Additive Genes ◽

Selection Of ◽

Hybrid Forms

The genotype–environment interactions and the inheritance of quantitative traits in a hybrid combination between a seeded grapevine cultivar and a seedless one (Armira × Rusalka 1) have been studied. It has been found that the selection of valuable hybrid forms shall become more efficient when conducted according to the traits ‘flowering – berry softening’, ‘berry softening – technological maturity’, ‘cluster weight’, ‘weight of 100 berries’ and ‘acids’. Dominant gene interactions with the environment are characterized by significantly greater intensity in comparison to those of additive genes. Inheritance in F1 progeny manifests very high values for the traits ‘budding’, ‘flowering’, ‘budding – flowering’, ‘flowering – berry softening’, ‘berry softening – technological maturity’, ‘berry length’. ‘berry width’, ‘weight of 100 berries’, ‘sugars’ and ‘acids’. According to the genetic stability of the dominant parameter interacting with the environment, a comparative evaluation can be made for each trait and, depending on the selection purpose, elite hybrid plants can be singled out, which possess a combination of the most important commercial traits.

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Insights into the Ecology and Evolution of Blattodea-associated Ophiocordyceps

10.21203/rs.3.rs-68793/v1 ◽

2020 ◽

Author(s):

Joao Araujo ◽

G.M. Moriguchi ◽

S. Uchiyama ◽

N. Kinjo ◽

Yu Matsuura

Keyword(s):

Genetic Similarity ◽

Evolutionary History ◽

Ryukyu Archipelago ◽

Tropical Regions ◽

Phylogenetic Placement ◽

The Social ◽

Host Jump ◽

High Degree ◽

First Time ◽

A New Species

Abstract Entomopathogenic fungi are ubiquitous inhabitants of forests worldwide, remarkably in tropical regions. Among these fungi, one of the most abundant and diverse is the genus Ophiocordyceps. These fungi are particularly diverse and more commonly found parasitizing coleopteran, lepidopteran, hymenopteran and hemipteran insects. However, other insect orders are also parasitized by these fungi, for example the blattodeans (termites and cockroaches). Despite their ubiquity in nearly all environments insects occur, blattodeans are rarely found infected by filamentous fungi and thus, their ecology and evolutionary history remains obscure. In this study, we propose a new species of Ophiocordyceps infecting the social cockroaches Salganea esakii and S. taiwanensis, based on 16 years of collections and field observations, especially the Ryukyu Archipelago. We found a high degree of genetic similarity between specimens from different islands, infecting two Salganea species and that this relationship is ancient, likely not originated from a recent host jump. Furthermore, we found that Ophiocordyceps lineages infecting cockroaches evolved around the same time, at least twice, one from beetles and the other from termites. We have also investigated the evolutionary relationships between Ophiocordyceps and termites and present the phylogenetic placement of O. blattae for the first time.

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