scholarly journals Identification and quantification of phenolic compounds in fresh and processed table olives of cv. ‘Kalamata’

2021 ◽  
Vol 49 (2) ◽  
pp. 12394
Author(s):  
Constantinos SALIS ◽  
Ioannis E. PAPADAKIS ◽  
Marianna HAGIDIMITRIOU
Keyword(s):  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Analytical Methods ◽  
High Performance ◽  
Healthy Lifestyle ◽  
Array Detector ◽  
Table Olives ◽  
Age Related ◽  
The Impact ◽  
Identification And Quantification

Mediterranean diet is almost synonymous to the healthy lifestyle and diet nowadays. Some of the major components of the diet are the products of the olive tree, fruits and olive oil, which are classified as medical foods, due to their nutraceutical benefits and their protective properties against cancer, cardiovascular diseases, age-related diseases, neurodegenerative disorders and other diseases. The key contributors to these properties are the phenolic compounds such as hydroxytyrosol, tyrosol and oleuropein. Table olives are being processed with several methods in order to reduce the bitterness of the olive fruit and the impact of the processing on phenolic compounds has not been studied extensively. In the present study, changes in the concentration of the most important phenolic compounds were quantified in fresh, Greek-style and Spanish-style processed olive fruits of cv. ‘Kalamata’, using two different analytical methods for identification and quantification: high-performance liquid chromatography diode array detector (HPLC-DAD) and ultrahigh-performance liquid chromatography tandem mass spectrometry (LC-(ESI)-MS/MS). The phenolic compounds that were identified and quantified were hydroxytyrosol, tyrosol, verbascocide, rutin, oleuropein and luteolin. Both processing methods used altered the phenolic compounds concentration in ‘Kalamata’ olive fruits compared to untreated fruits. In both analytical methods, a statistically significant increase in verbascoside and hydroxytyrosol concentration and a statistically significant decrease in rutin concentration was observed in both, Greek-style and Spanish-style, processed olive fruits.

scholarly journals Schisandra rubriflora Plant Material and In Vitro Microshoot Cultures as Rich Sources of Natural Phenolic Antioxidants

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 488
Author(s):  
Agnieszka Szopa ◽  
Michał Dziurka ◽  
Sebastian Granica ◽  
Marta Klimek-Szczykutowicz ◽  
Paweł Kubica ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Phenolic Acids ◽  
High Performance ◽  
Total Phenolic ◽  
Array Detector ◽  
Plant Materials

Schisandra rubriflora is a dioecious, underestimated medicinal plant species known from traditional Chinese medicine. The present study was aimed at characterising the polyphenolic profile composition and the related antioxidant capacity of S. rubriflora fruit, stem and leaf and in vitro microshoot culture extracts. Separate analyses of material from female and male specimens were carried out. This study was specifically aimed at detailed characterisation of the contribution of phenolic compounds to overall antioxidant activity using ultra-high-performance liquid chromatography with a photodiode array detector coupled to electrospray ionization ion trap mass spectrometry (UHPLC-DAD-ESI-MS3) and a high-performance liquid chromatography-diode array detector (HPLC-DAD). Using UHPLC-DAD-ESI-MS3, twenty-seven phenolic compounds from among phenolic acids and flavonoids were identified. Concentrations of three phenolic acids (neochlorogenic, chlorogenic and cryptochlorogenic acids) and eight flavonoids (hyperoside, rutoside, isoquercitrin, guaijaverin, trifolin, quercetin, kaempferol, and isorhamnetin) were determined using HPLC-DAD using reference standards. The highest total phenolic content was confirmed for the stem and leaf extracts collected in spring. The contents of phenolic compounds of in vitro biomasses were comparable to that in the fruit extracts. The methanolic extracts from the studied plant materials were evaluated for their antioxidant properties using various in vitro assays, namely free radicals scavenging estimation using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), ferric-reducing antioxidant power (FRAP) and cupric-reducing antioxidant capacity (CUPRAC) as well as QUick, Easy, New, CHEap, and Reproducible CUPRAC (QUENCHER-CUPRAC) assays. A close relationship between the content of polyphenolic compounds in S. rubriflora and their antioxidant potential has been documented.

scholarly journals Determination and Quantification of Gallic Acid in Raw Propolis by High-performance Liquid Chromatography–Diode Array Detector in Burundi

2019 ◽  
Vol 1 (1) ◽  
pp. 43-48
Author(s):  
Ramadhan Nyandwi ◽  
Ayşe S. Kılıç ◽  
Meltem Çelik ◽  
Hasan H. Oruç
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Caffeic Acid ◽  
High Performance ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Sub Saharan

Background: Honey, pollen, and propolis are among the products that bees process and derive from plants and flowers. Propolis is a resinous material that bees gather from the buds and bark of some trees and small plants. Propolis from temperate climates mainly contains phenolic compounds, in contrast with propolis from tropical climates, which mainly contains terpenes. This study aimed to determine, characterise, and quantify the phenolic content of raw propolis from Burundi. Methods: In this study, a total of 6 samples were collected from the provinces of Rumonge, Cibitoke, and Ruyigi in Burundi. Fifteen phenolic compounds (caffeic acid, ferulic acid, epigallocatechin gallate, isoferulic acid, cinnamic acid, caffeic acid phenethyl ester, gallic acid, apigenin, chrysin, galangin, quercetin, kaempherol, rutin trihydrate, naringenin, and pinocembrin) were used as high-performance liquid chromatography (HPLC) standards for qualitative and quantitative analyses of the propolis samples. Results: Among the 15 phenolic compounds checked, only 1 – gallic acid – was detected at a measurable level using an HPLC-diode array detector system. Conclusion: In addition to terpenes, propolis found in sub-Saharan Africa may contain phenolic compounds. Further advanced investigation of sub-Saharan African propolis is required for more detailed characterisation.

Quantification of Phenolic Compounds from Fadogia agrestis and Dietary Supplements using UHPLC-PDA-MS

Planta Medica ◽  
2018 ◽  
Vol 85 (02) ◽  
pp. 145-153 ◽  
Author(s):  
Bharathi Avula ◽  
Ji-Yeong Bae ◽  
Vijayasankar Raman ◽  
Omer Fantoukh ◽  
Yan-Hong Wang ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Dietary Supplements ◽  
High Performance ◽  
Chemical Constituents ◽  
Array Detector ◽  
Gradient System ◽  
Chromatography Method ◽  
Limits Of Detection ◽  
Positive Mode

Abstract Fadogia agrestis is used in traditional African medicine as an analgesic and for anti-inflammatory and aphrodisiac activities. An ultra-high-performance liquid chromatography method was developed for the determination of 11 chemical constituents from roots and aerial parts of F. agrestis. The separation was achieved within 7 min by using C-18 column material and a water/acetonitrile mobile phase, both containing 0.1% formic acid gradient system with a temperature of 45 °C. The method was validated for linearity, repeatability, limits of detection, and limits of quantification. The limits of detection of phenolic compounds were found to be in the range from 0.025 to 0.1 µg/mL. The wavelengths used for quantification with the photodiode array detector were 238, 254, 291, and 325 nm. Twelve of 17 dietary supplements contained phenolic compounds in the range from 0.3 to 2.7 mg/d. The phenolic compounds were not detected in five dietary supplements. Liquid chromatography-mass spectrometry coupled with electrospray ionization interface method is described for the identification and confirmation of compounds from plant samples and dietary supplements claiming to contain F. agrestis. This method involved the use of [M + H]+ and [M + Na]+ ions in the positive mode and [M − H]− ions in the negative mode with extractive ion monitoring. The developed method is simple, economic, rapid, and especially suitable for quality control and chemical fingerprint analysis of F. agrestis.

scholarly journals Quality Assessment of Ojeok-San, a Traditional Herbal Formula, Using High-Performance Liquid Chromatography Combined with Chemometric Analysis

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Jung-Hoon Kim ◽  
Chang-Seob Seo ◽  
Seong-Sil Kim ◽  
Hyeun-Kyoo Shin
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quality Assessment ◽  
Analytical Methods ◽  
High Performance ◽  
Water Extract ◽  
Chemometric Analysis ◽  
Array Detector ◽  
Herbal Formula ◽  
Marker Compounds

Ojeok-san (OJS) is a traditional herbal formula consisting of 17 herbal medicines that has been used to treat various disorders. In this study, quantitative analytical methods were developed using high-performance liquid chromatography equipped with a photodiode array detector to determine 19 marker compounds in OJS preparations, which was then combined with chemometric analysis. The method developed was validated in terms of its precision and accuracy. The intra- and interday precision of the marker compounds were <3.0% of the relative standard deviation (RSD) and the recovery of the marker compounds was 92.74%–104.16% with RSD values <3.0%. The results of our quantitative analysis show that the quantities of the 19 marker compounds varied between a laboratory water extract and commercial OJS granules. The chemometric analysis used, principal component analysis (PCA) and hierarchical clustering analysis (HCA), also showed that the OJS water extract produced using a laboratory method clearly differed from the commercial OJS granules; therefore, an equalized production process is required for quality control of OJS preparations. Our results suggest that the HPLC analytical methods developed are suitable for the quantification and quality assessment of OJS preparations when combined with chemometric analysis involving PCA and HCA.

scholarly journals Screening and Characterization of Phenolic Compounds from Australian Grown Bananas and Their Antioxidant Capacity

Antioxidants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1521
Author(s):  
Yasmeen M. Bashmil ◽  
Akhtar Ali ◽  
Amrit BK ◽  
Frank R. Dunshea ◽  
Hafiz A. R. Suleria
Keyword(s):  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
High Performance ◽  
Antioxidant Activities ◽  
Banana Peel ◽  
Array Detector ◽  
Total Polyphenols ◽  
Photodiode Array Detector ◽  
Photodiode Array

Bananas are an essential source of staple food and fruit worldwide and are widely regarded as the world’s largest fruit crop, with more than 100 million tons total annual production. Banana peel, a by-product that represents about 40% of the entire banana’s weight, and pulp are rich in bioactive compounds and have a high antioxidant capacity. As the production of polyphenols in fruit and vegetables is highly dependent on environmental conditions, genetic factors, and the level of maturity, this study aims to characterize six Australian banana cultivars in various stages of ripening for their phenolic compounds using the liquid chromatography-electrospray ionization quadrupole time of flight mass spectrometry (LC-ESI-QTOF-MS/MS), polyphenols quantification with the high-performance liquid chromatography coupled with photodiode array detector (HPLC-PDA), and their antioxidant capacity. All bananas were analysed for total polyphenols content (TPC), total flavonoids content (TFC), and total tannin content (TTC) and their antioxidant activities. Ripe Ducasse peel and pulp contained the highest amounts of total polyphenols content (1.32 and 1.28 mg gallic acid equivalent (GAE) per gram of sample), total tannin contents (3.34 mg catechin equivalent (CE) per gram of sample), and free radical scavenging capacity (106.67 mg ascorbic acid equivalent (AAE) per g of sample). In contrast, ripe Plantain peel had the greatest total flavonoids (0.03 mg quercetin equivalent (QE) per g of sample). On the other hand, unripe Ladyfinger pulp possessed the highest total antioxidant activity (1.03 mg AAE/g of sample). There was a positive correlation between flavonoids and antioxidant activities. By using LC-ESI-QTOF-MS/MS, a total of 24 phenolic compounds were tentatively characterized in this research, including six phenolic acids, 13 flavonoids, and five other polyphenols. Quantification of phenolic compounds by the high-performance liquid chromatography coupled with photodiode array detector (HPLC-PDA) revealed a higher content of phenolic acids. These findings confirmed that banana peel and pulp have considerable antioxidant activity and can be employed in human food and animal feed for variant health enhancement uses.

Determination and Quantification of Gallic Acid in Raw Propolis by High-performance Liquid Chromatography–Diode Array Detector in Burundi

2019 ◽  
Vol 1 (1) ◽  
pp. 43-48
Author(s):  
Ramadhan Nyandwi ◽  
Ayşe S. Kılıç ◽  
Meltem Çelik ◽  
Hasan H. Oruç
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Caffeic Acid ◽  
High Performance ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Sub Saharan

Background: Honey, pollen, and propolis are among the products that bees process and derive from plants and flowers. Propolis is a resinous material that bees gather from the buds and bark of some trees and small plants. Propolis from temperate climates mainly contains phenolic compounds, in contrast with propolis from tropical climates, which mainly contains terpenes. This study aimed to determine, characterise, and quantify the phenolic content of raw propolis from Burundi. Methods: In this study, a total of 6 samples were collected from the provinces of Rumonge, Cibitoke, and Ruyigi in Burundi. Fifteen phenolic compounds (caffeic acid, ferulic acid, epigallocatechin gallate, isoferulic acid, cinnamic acid, caffeic acid phenethyl ester, gallic acid, apigenin, chrysin, galangin, quercetin, kaempherol, rutin trihydrate, naringenin, and pinocembrin) were used as high-performance liquid chromatography (HPLC) standards for qualitative and quantitative analyses of the propolis samples. Results: Among the 15 phenolic compounds checked, only 1 – gallic acid – was detected at a measurable level using an HPLC-diode array detector system. Conclusion: In addition to terpenes, propolis found in sub-Saharan Africa may contain phenolic compounds. Further advanced investigation of sub-Saharan African propolis is required for more detailed characterisation.

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