scholarly journals Evaluation of three plant species to control black scurf disease of Irish potato (Solanum tuberosum Linn.)

2020 ◽  
Vol 12 (1) ◽  
pp. 90-99
Author(s):  
Matthew O. ADEBOLA ◽  
Tunde S. BELLO ◽  
Esther A. SERIKI ◽  
Mariam B. AREMU
Keyword(s):  
Solanum Tuberosum ◽  
Mycelial Growth ◽  
Pathogenic Fungus ◽  
Inhibitory Effect ◽  
Black Scurf ◽  
Irish Potato ◽  
Diffusion Method ◽  
Leaf Extracts

Irish potato (Solanum tuberosum) is an important worldwide food crop and one of the most popular in Nigeria. Its abundance and successful yield have been immensely affected by black scurf disease caused by Rhizoctonia solani. Harnessing a cost-effective management of this pathogenic fungus, three botanical species Acalypha wilkesiana, Moringa oleifera and Carica papaya leaves, each at concentrations of 0 mg ml-1 (control), 25 mg ml-1, 50 mg ml-1 and 75 mg ml-1 were evaluated in vitro. The plant leaf extracts were prepared using methanol and were evaluated for their toxicity using agar well diffusion method. The fungus was isolated from spoilt Irish potato with black scurf symptoms. The results showed the presence of some phytochemicals in leaf extract of each of the plants tested. The three leaves extract independently inhibited mycelial growth of R. solani. The potency of all the plant extracts increased with the increase in concentration. The highest concentration (75 mg ml-1) of M. oleifera and C. papaya evaluated, gave the highest inhibitory effect of 0.81 mm and 1.63 mm respectively, which were not significantly different (p> 0.05), but was obviously different from A. wilkesiana (2.81 mm). Furthermore, M. oleifera extract gave the highest percentage of mycelial growth inhibition of the fungus in all grades of the concentrations evaluated, whereas A. wilkesiana showed the least. The leaves of the three species are therefore recommended for in vivo control of this fungus, owing to their proven efficacy and to their cheap availability.

scholarly journals Inhibition of Mycelial Growth of Rhizoctonia Solani by Chitosan in vitro and in vivo

2019 ◽  
Vol 13 (1) ◽  
pp. 156-161
Author(s):  
Sabah R. Mohammed ◽  
Elsayed M. Zeitar ◽  
Ivan D. Eskov
Keyword(s):  
Rhizoctonia Solani ◽  
Mycelial Growth ◽  
Phenylalanine Ammonia Lyase ◽  
Inhibitory Effect ◽  
Black Scurf ◽  
Potato Tubers ◽  
Antifungal Effect ◽  
Poisoned Food Technique

Objective: Evaluate the antifungal effect of chitosan against Rhizoctonia solani in vitro and the possible mechanisms of its induced activity in potato tubers to control black scurf disease. Methods: The in vitro influence of chitosan at different concentrations on mycelial growth of R. solani was tested by using the poisoned food technique in PDA medium. The effect of these concentrations on the development of lesion diameters in tubers inoculated with R. solani mycelium was assayed for 30 days. The concentration that showed the greatest inhibitory effect on lesion diameters was tested to assess the induced activity of defense-related enzymes in the infected tubers. Results: In the poisoned food technique, chitosan at 1% completely inhibited the growth of R. solani mycelium. In vivo tests showed that chitosan treatment at 0.5% effectively controlled the black scurf in tubers inoculated with R. solani mycelium. Chitosan increased the activities of defense-related enzymes such as Peroxidase (POD), Polyphenol Oxidase (PPO) and Phenylalanine Ammonia-lyase (PAL) in treated tubers of tested cultivars. Conclusion: This work demonstrated that chitosan directly inhibited the growth of R. solani, and potentially elicited defense reaction in potato tubers.

Antifungal activity of plant extracts against Colletotrichum musae, the post harvest anthracnose pathogen of banana cv. Martaman

2016 ◽  
Vol 46 (1) ◽  
pp. 2-15 ◽  
Author(s):  
Dawa Dolma Bhutia ◽  
Yeka Zhimo ◽  
Ramen Kole ◽  
Jayanta Saha
Keyword(s):  
Antifungal Activity ◽  
Plant Species ◽  
Plant Extract ◽  
Mycelial Growth ◽  
Disease Incidence ◽  
Leaf Extracts ◽  
Content Type ◽  
Colletotrichum Musae

Purpose – The purpose of this paper was to determine the antifungal activities of different solvent extracts of common plants in vitro and in vivo against banana anthracnose fungus Colletotrichum musae (Berk & M.A. Curtis) Arx, and to investigate its effects on the pathogen and identify the bio active component(s). Design/methodology/approach – Extracts were obtained from leaves, tender shoots, rhizomes, bulbs, seeds and fruits of 42 naturally growing plant species following hot sequential extraction. Preliminary screening of the solvent extracts was done based on the inhibition of radial mycelial growth of C. musae following poison food technique and conidial germination inhibition by cavity slide technique. The selected extracts were assessed for their effect on harvested banana in reducing anthracnose during storage. The active components in the bio-active fractions of plant extract were identified by gas chromatography-mass spectroscopy. Findings – Methanol extracted a larger quantity of material (between 6.9 and 12.5 per cent) than hexane or chloroform, and all its extracts were active against the test pathogen with mycelial growth inhibition ranging from 13.70 to 88.89 per cent. Zingiber officinale rhizome extract as well as Polyalthia longifolia and Clerodendrum inerme leaf extracts exhibited more than 80 per cent inhibition of mycelial growth. Total inhibition of spore germination of C. musae was recorded in Z. officinale and P. longifolia extracts at 0.3 per cent w/v and 0.5 per cent w/v concentration, respectively, while only 68 per cent spore inhibition was recorded in C. inerme at 0.5 per cent w/v concentration. Of the three plant species, Z. officinale had the best antifungal activity (18.0 per cent disease incidence; 2.2 disease severity scale) when banana fruits were dipped in the extract at a concentration of 0.5 per cent w/v at 5 days of storage in ambient condition (80-82 per cent R.H., 27 ± 1°C). The bio-active compounds in the extract of Z. officinale were identified as alpha-curcumene and zingerone. Originality/value – Based on the antifungal activity, plant extract of Z. officinale can be used as an effective alternative to chemicals in controlling anthracnose pathogen in harvested banana.

Anticandidal Activity of Cratoxylum formosum Gum and its Cytotoxicity

2014 ◽  
Vol 974 ◽  
pp. 394-397 ◽  
Author(s):  
Sroisiri Thaweboon ◽  
Boonyanit Thaweboon ◽  
Surachai Dechkunakorn ◽  
Passiri Nisalak ◽  
Rattiporn Kaypetch
Keyword(s):  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Dilution Method ◽  
Mountainous Area ◽  
Cytotoxicity Test ◽  
Oral Diseases ◽  
Zones Of Inhibition

Cratoxylumformosumis a plant widely distributed in mountainous area of various Asian countries. The extract prepared from the burnt bark has been used among the local people as a varnish to prevent tooth decay and other oral diseases. The aim of this study was to examine antifungal activity ofC. formosumgum againstCandidaalbicansand to evaluate its cytotoxicity. The gum prepared from the extract ofC.formosumwas investigated for antimicrobial activity against 3 strains ofC.albicans. Inhibition of microbial growth was primarily tested by agar diffusion method. A two-fold broth dilution method was then used to determine the minimum inhibitory concentration (MIC) of the gum. Based on the MIC value, cytotoxicity test was performed on mouse fibroblasts (ATCC clone 929) using agar overlay technique. Inhibitory effect of the gum was seen againstC. albicanswith zones of inhibition ranging from 8.0 to 9.3 mm. MIC values were between 0.50 and 1.25 mg/mL. In term of cytotoxicity,C. formosumgum at the concentration of 20 MIC (25 mg/mL) was classified as grade 3 (moderate cytotoxicity) whereas those of 10 MIC and 1 MIC were grade 1 (slight cytotoxicity). In conclusion, the gum prepared fromC. formosumextract exhibited antimicrobial activities against all the test strains ofC. albicans. From the present study, it can be suggested that this plant can be used as a novel antifungal agent, effective againstC.albicansinfections, due to its inhibitory effects onC. albicansand acceptable biocompatibility. Furtherin vitro/in vivostudies should be conducted to understand the mechanisms of action and to establish the safe profile of this gum for clinical usage.

scholarly journals UTILIZATION OF SAMBUNG NYAWA LEAF EXTRACTS Gynura procumbens (Lour) Merr. FOR TREATMENT OF Vibrio alginolyticus IN TIGER GROUPER (Epinephelus fuscoguttatus Forsskal, 1775)

2019 ◽  
Vol 47 (2) ◽  
pp. 134
Author(s):  
Novi Santika ◽  
Wardiyanto Wardiyanto ◽  
Esti Harpeni
Keyword(s):  
Leaf Extract ◽  
Sea Water ◽  
Economic Value ◽  
In Vitro Study ◽  
Inhibitory Effect ◽  
Vibrio Alginolyticus ◽  
Leaf Extracts ◽  
Tiger Grouper

Tiger Grouper is one of the sea water fish commodities that is quite popular with the community and has a high economic value. The problem faced by farmers is the attack of Vibriosis, one of which is caused by the Vibrio alginolyticus bacteria. The use of synthetic antibiotics has been widely used but has many adverse effects, so it needs new alternatives for the treatment of Vibriosis disease. One of them is by using the extract of lifelong leaf extract. Life-sustaining plants (Gynura procumbens) contain secondary metabolites such as flavonoids, tannins, and antibacterial saponins. This study aims to determine the best dosage of lifelong leaf extract for the treatment of Vibriosis disease in tiger grouper. The study was conducted in two stages, namely in vitro and in vivo. Before the fish were treated with feed that had been given a sambung deca leaf extract, the fish were challenged using Vibrio alginolyticus with a density of 108 CFU / mL as much as 0.1 mL / head and then fed with treatment and maintained for 21 days. The results of the in vitro study showed that the life of sambung leaf extract at a dose of 700 ppm had a broad inhibitory effect on V. alginolyticus, which amounted to 10.47 mm compared to other treatments. Whereas when continued for in vivo testing, a dose of 350 ppm in general has been applied for the treatment of attacks of Vibrio alginolyticus in tiger grouper.

scholarly journals The Antimicrobial Activities of Psidium guajava and Juglans regia Leaf Extracts to Acne-Developing Organisms

2005 ◽  
Vol 33 (02) ◽  
pp. 197-204 ◽  
Author(s):  
Fadi Qa'dan ◽  
Abdul-Jalil Thewaini ◽  
Dalia A. Ali ◽  
Rana Afifi ◽  
Abdalla Elkhawad ◽  
...  
Keyword(s):  
Juglans Regia ◽  
Psidium Guajava ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Leaf Extracts ◽  
Disk Diffusion Method ◽  
Tea Tree ◽  
Zones Of Inhibition

This study aims to present the in vitro inhibitory effect of Psidium guajava and Juglans regia leaf extracts on the main developer of acne lesions, Propionibacterium acnes (P. acnes), and other organisms that are isolated from acne lesions. Thirty-eight subjects (males and females) who had various types of acne were enrolled in the study. The contents of the acne lesions were cultured and the frequency of P. acnes (alone and with Staphylococci spp.) was 47%, whereas the frequencies for Staphylococcus aureus and Staphylococcus epidermidis were 13% and 24%, respectively. The antimicrobial activities of Psidium guajava and Juglans regia leaf extracts, determined by disk diffusion method (zone of inhibition), were compared to tea tree oil (TTO), doxycycline and clindamycin antibiotics. The zones of inhibition due to the Psidium guajava and Juglans regia leaf extracts ranged from 15.8–17.6 mm against P. acnes, 11.3–15.7 mm against S. aureus and 12.9–15.5 mm against S. epidermidis, respectively. These zones of inhibition were significantly higher than those of TTO and equivalent in case of Staphylococci spp., but less in case of P. acnes, to those obtained from doxycycline or clindamycin. It can be concluded that Psidium guajava and Juglans regia leaf extracts may be beneficial in treating acne especially when they are known to have anti-inflammatory activities.

The Effect of Barbituric Acid Derivatives on Platelet Function in Vitro and in Vivo

1973 ◽  
Vol 30 (02) ◽  
pp. 315-326
Author(s):  
J. Heinz Joist ◽  
Jean-Pierre Cazenave ◽  
J. Fraser Mustard
Keyword(s):  
Platelet Aggregation ◽  
Platelet Function ◽  
Barbituric Acid ◽  
Platelet Rich Plasma ◽  
Inhibitory Effect ◽  
Primary Response ◽  
Sodium Pentobarbital ◽  
Barbituric Acid Derivatives

SummarySodium pentobarbital (SPB) and three other barbituric acid derivatives were found to inhibit platelet function in vitro. SPB had no effect on the primary response to ADP of platelets in platelet-rich plasma (PRP) or washed platelets but inhibited secondary aggregation induced by ADP in human PRP. The drug inhibited both phases of aggregation induced by epinephrine. SPB suppressed aggregation and the release reaction induced by collagen or low concentrations of thrombin, and platelet adherence to collagen-coated glass tubes. The inhibition by SPB of platelet aggregation was readily reversible and isotopically labeled SPB did not become firmly bound to platelets. No inhibitory effect on platelet aggregation induced by ADP, collagen, or thrombin could be detected in PRP obtained from rabbits after induction of SPB-anesthesia.

Inhibition of Human and Animal Platelet Adhesiveness to Glass Bead Columns by Adenosine, Dipyridamole, Chlorpromazine and Acetylsalicylic Acid

1976 ◽  
Vol 36 (02) ◽  
pp. 401-410 ◽  
Author(s):  
Buichi Fujttani ◽  
Toshimichi Tsuboi ◽  
Kazuko Takeno ◽  
Kouichi Yoshida ◽  
Masanao Shimizu
Keyword(s):  
Guinea Pig ◽  
Acetylsalicylic Acid ◽  
Guinea Pigs ◽  
Glass Bead ◽  
Animal Species ◽  
Inhibitory Effect ◽  
Rabbit Platelet ◽  
Platelet Adhesiveness

SummaryThe differences among human, rabbit and guinea-pig platelet adhesiveness as for inhibitions by adenosine, dipyridamole, chlorpromazine and acetylsalicylic acid are described, and the influence of measurement conditions on platelet adhesiveness is also reported. Platelet adhesiveness of human and animal species decreased with an increase of heparin concentrations and an increase of flow rate of blood passing through a glass bead column. Human and rabbit platelet adhesiveness was inhibited in vitro by adenosine, dipyridamole and chlorpromazine, but not by acetylsalicylic acid. On the other hand, guinea-pig platelet adhesiveness was inhibited by the four drugs including acetylsalicylic acid. In in vivo study, adenosine, dipyridamole and chlorpromazine inhibited platelet adhesiveness in rabbits and guinea-pigs. Acetylsalicylic acid showed the inhibitory effect in guinea-pigs, but not in rabbits.

An Attempt at Biological Control of Blossom Blight of Rose Caused by Botrytis cinerea Using some Local Trichoderma spp. Strains

2020 ◽  
Vol 55 (1) ◽  
pp. 27-34
Author(s):  
G. Zadehdabagh ◽  
K. Karimi ◽  
M. Rezabaigi ◽  
F. Ajamgard
Keyword(s):  
Botrytis Cinerea ◽  
Mycelial Growth ◽  
Limiting Factor ◽  
Dual Culture ◽  
Trichoderma Spp ◽  
Khuzestan Province ◽  
Inhibitory Potential ◽  
Cut Rose

The northern of Khuzestan province in Iran is mainly considered as one of the major areas of miniature rose production. Blossom blight caused by Botrytis cinerea has recently become a serious limiting factor in rose production in pre and post-harvest. In current study, an attempt was made to evaluate the inhibitory potential of some local Trichoderma spp. strains against B. cinerea under in vitro and in vivo conditions. The in vitro results showed that all Trichoderma spp. strains were significantly able to reduce the mycelial growth of the pathogen in dual culture, volatile and non-volatile compounds tests compared with control, with superiority of T. atroviride Tsafi than others. Under in vivo condition, the selected strain of T. atroviride Tsafi had much better performance than T. harzianum IRAN 523C in reduction of disease severity compared with the untreated control. Overall, the findings of this study showed that the application of Trichoderma-based biocontrol agents such as T. atroviride Tsafi can be effective to protect cut rose flowers against blossom blight.

Preliminary in Vitro Investigations on The Inhibitory Activity of The Original Dietary Supplement Oxidal® on Pathogenic Bacterial Strains

2020 ◽  
Vol 11 ◽  
pp. 37-43
Author(s):  
Prof. Teodora P. Popova ◽  
Toshka Petrova ◽  
Ignat Ignatov ◽  
Stoil Karadzhov
Keyword(s):  
Dietary Supplement ◽  
Broad Spectrum ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Clinical Effectiveness ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Microbial Strains

The antimicrobial action of the dietary supplement Oxidal® was tested using the classic Bauer and Kirby agar-gel diffusion method. Clinical and reference strains of Staphylococcus aureus and Escherichia coli were used in the studies. The tested dietary supplement showed a well-pronounced inhibitory effect against the microbial strains commensurable with that of the broad-spectrum chemotherapeutic agent Enrofloxacin and showed even higher activity than the broad spectrum antibiotic Thiamphenicol. The proven inhibitory effect of the tested dietary supplement against the examined pathogenic bacteria is in accordance with the established clinical effectiveness standards for antimicrobial agents.

Phenolic Acids Exert Anticholinesterase and Cognition-Improving Effects

2018 ◽  
Vol 15 (6) ◽  
pp. 531-543 ◽  
Author(s):  
Dominik Szwajgier ◽  
Ewa Baranowska-Wojcik ◽  
Kamila Borowiec
Keyword(s):  
Phenolic Acids ◽  
Ex Vivo ◽  
Amyloid Β ◽  
Inhibitory Effect ◽  
In Vivo Studies ◽  
Amyloid Β Peptide ◽  
Beneficial Effects ◽  
Aβ Fibrils

Numerous authors have provided evidence regarding the beneficial effects of phenolic acids and their derivatives against Alzheimer's disease (AD). In this review, the role of phenolic acids as inhibitors of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) is discussed, including the structure-activity relationship. In addition, the inhibitory effect of phenolic acids on the formation of amyloid β-peptide (Aβ) fibrils is presented. We also cover the in vitro, ex vivo, and in vivo studies concerning the prevention and treatment of the cognitive enhancement.

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