Acetyl salicylic acid alleviates chilling-induced damage in muskmelon seedlings

2007 ◽  
Vol 87 (3) ◽  
pp. 581-585 ◽  
Author(s):  
Ahmet Korkmaz ◽  
Murat Uzunlu ◽  
Ali Riza Demirkiran

Salicylic acid (SA) is a common plant-produced signal molecule that is responsible for inducing tolerance to a number of biotic and abiotic stresses. An experiment was, therefore, conducted to test whether acetyl salicylic acid (ASA) application at various concentrations through seed immersion or foliar spray would protect muskmelon [Cucumis melo L. (Reticulatus Group)] seedlings subjected to chilling stress. Twenty-one-day-old plants pre-treated with ASA (0, 0.1, 0.25, 0.50 or 1.0 mM) were subjected to chilling stress for 72 h at 3 ± 0.5°C. ASA, applied either through seed immersion or foliar spray, was effective within the range of 0.1 to 1 mM in inducing tolerance to chilling stress in muskmelon seedlings; however, there was no significant difference between application methods. ASA significantly and curvilinearly affected all seedling growth and stress indicator variables tested except shoot dry weight. The best protection was obtained from seedlings pre-treated with 0.5 mM ASA. The highest ASA concentration used was slightly less effective in providing chilling stress protection. Even though both methods provided similar means of protection, due to its simplicity and practicality, immersion of muskmelon seeds prior to sowing in 0.5 mM ASA would be a more desirable method to induce tolerance to chilling stress. Key words: Cucumis melo, aspirin, chilling stress tolerance, gas exchange, electrolyte leakage

1990 ◽  
Vol 70 (3) ◽  
pp. 925-930 ◽  
Author(s):  
PETER R. HICKLENTON

This study investigated the effects of growth retardants uniconazole [(E) - (p-chlorohenyl) -4, 4-dimethyl-2-(1,2,4-triazol-1-yl)-1-penten-3-ol] and daminozide (butanedioic acid mono 2,2-dimethylhydrazide) on three chrysanthemum (Dendranthema grandiflora Tzvelev.) cultivars. Uniconazole applied as a soil drench (0.02 mg a.i. pot−1) or foliar spray (0.014 mg a.i. pot−1) 10 d after removal of the shoot tip reduced plant height at harvest in cultivars Deep Luv, Tip and Tara. Higher doses of uniconazole resulted in further plant height reduction in Tip and Tara but not in Deep Luv. Daminozide spray (14 mg a.i. pot) and uniconazole spray (0.028 or 0.056 mg a.i. pot−1) produced plants of similar height. Pre-plant dips of both growth retardants were less effective than sprays or drenches in controlling height. Flower area and flower dry weight were reduced with uniconazole drench and spray, and daminozide spray in each cultivar. Shoot dry weight was similarly affected in Tip and Tara but not in Deep Luv. Flowering was delayed in each cultivar by post-plant treatments of uniconazole drench and spray and by daminozide spray (0.08, 0.014 and 14 mg a.i. pot−1, respectively), and by daminozide and uniconazole pre-plant dips (5.0 mg L−1 and 4000 mg L−1, respectively).Key words: Sumagic, XE-1019, B-Nine, Alar, Chrysanthemum × morifolium, Dendanthema grandiflora


HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 521B-521 ◽  
Author(s):  
Fahed A. Al-mana ◽  
David J. Beattie

A study of applying growth retardants under overhead and subsurface irrigation systems was conducted on bermudagrass (Cynodon dactylon L. cv. Tifway) grown from rhizomes in 15-cm pots containing sand medium. Paclobutrazol (50%) at 2 mg/pot was used as foliar spray or charged-hydrophilic polymers (Super Sorb C) and either incorporated or put below medium surface. Mefluidide (28%) at 0.01% ml/pot was used only as foliar spray. Before spray treatments, grasses were cut at 2 cm from medium surface, and the second cut was made at the 6th week from treatment. All growth retardant treatments reduced grass height compared to non-treated plants. The lowest grass height was produced by paclobutrazol as foliar spray under overhead irrigation in the 6th and 9th week. By the 9th week, all hormonal treatments under the two irrigation systems had no effect on grass quality, color, and establishment rate. Both paclobutrazol foliar spray and below medium surface charged-polymer treatments under subsurface irrigation had the lowest water loss and dry weight by the 6th and 9th week. The paclobutrazol charged-polymer treatment under subsurface irrigation had also the the lowest root dry weight among all treatments. Although mefluidide foliar spray was less effective on grass height than paclobutrazol, they had similar effect on water loss and shoot dry weight.


Author(s):  
Dwi Astutik ◽  
Ratih Rahhutami ◽  
Aline Sisi Handini ◽  
Ahmad Sutopo

<em>A planting medium that rich in nutrients is needed for oil palm, especially in the nursery phase. This study aimed to determine the effect of various doses of EM4 and to get the best dose of EM4 on the growth of oil palm seedlings in the pre-nursery. The research method used was a non-factorial completely randomized design with 4 treatments, namely 0 ml EM4 (A1), 4 ml EM4 (A2), 8 ml EM4 (A3), and 12 ml EM4 (A4). Each treatment was repeated 6 times so that there were 24 experimental units. The data obtained were analyzed for variance at the 5% level, if the effect was real, it was followed by the least significant difference (LSD) test. Based on the results of the study, it could be concluded that application of various EM4 doses had a significant effect on plant height (4-12 MST), the number of leaves (4-8 MST), trunk girth (4-12 MST), and shoot wet weight but did not significantly affect the number of leaves (12 MST), the volume of roots, length of roots, root wet weight and shoot dry weight, dry weight root, the best dose was shown at a dose of 12 ml EM4.</em>


2021 ◽  
Vol 6 (1) ◽  
pp. 38
Author(s):  
Riza Afrinda ◽  
Budiastuti Kurniasih

Marginal land along the coast should be utilized to increase the national rice production. In addition, the use of saline resistant varieties, it is necessary to determine the best seedling age for saline soil. This study aimed to evaluate the growth and yield responses of two rice varieties transplanted at different seedling age on saline soil. The experiment was arranged in a factorial Randomized Completely Block Design (RCBD) with three replications, conducted at saline coastal area of Baros, Yogyakarta, starting from January to June 2017. The first factor was rice varieties, consisting of Dendang and IR-64. Meanwhile, the second factor was the seedling age, consisting of 2 and 4 weeks after sowing (WAS) seedlings. The results showed that the growth and yield of two rice varieties (Dendang and IR-64) showed significant difference, and Dendang showed a better performance than IR-64. The higher yield of Dendang compared to IR-64 was supported by higher plant growth (shoot dry weight) and yield components (number of seeds per panicle, seed weight per plot, and productivity). The 2 and 4 WAS seedlings did not give significantly different effects on nearly all growth variables. However, 2 WAS Dendang rice seedlings had higher shoot dry weight. Meanwhile, IR-64 had higher leaf greenness in 4 WAS seedlings compared to that in 2 WAS seedlings.


2021 ◽  
Vol 9 (3) ◽  
pp. 262
Author(s):  
Alawiyah Alawiyah ◽  
Slamet Budi Yuwono ◽  
Melya Riniarti ◽  
Dermiyati Dermiyati ◽  
Christine Wulandari

Open mining activities have caused soil damage, water pollution, air pollution, and damage to vegetation. Limestone post mining is a critical area that must be rehabilitated to be productive again. This problems can be fixed by doing land reclamation activities which mandated in the Mining Law Number 4, 2009. The success of land reclamation is largely determined by the choice of plant species and the provision of appropriate soil enhancers (ameliorant). This study was aimed to analyze the growth response of sengon plants on giving ameliorant in mixed limestone post mining soil. This study was designed in completely randomized design for 7 treatments and 3 replications used combination of ameliorant: P0 (without ameliorant); P1 (AH 4 kg ha-1); P2 (BFA 350 kg ha-1); P3 (FMA 500 spores plant-1); P4 (the combination of AH and FMA); P5 (the combination of BFA and FMA); and P6 (the combination of AH, BFA, and FMA). Data were processed by analysis of variance at the 0,05 significance level followed by the Least Significant Difference test. The results showed that the best growth response of sengon on giving ameliorant in mixed limestone post mining soil was P1 treatment (humic acid material) which significantly increased root length, root volume, root fresh weight, shoot fresh weight, root dry weight, and shoot dry weight in sengon plants. The addition of ameliorant can be recommended to improve the quality of revegetation plants


HortScience ◽  
2011 ◽  
Vol 46 (9) ◽  
pp. 1303-1307 ◽  
Author(s):  
Genhua Niu ◽  
Denise Rodriguez ◽  
Mengmeng Gu

Texas mountain laurel (Sophora secundiflora) is a native shrub tolerating drought, heat, windy conditions, and alkaline or wet soils. However, its availability is somewhat low and little information is available on nutrient requirement and other culture information. Two greenhouse experiments were conducted to quantify the responses of Texas mountain laurel to different forms and rates of nitrogen (N) fertilizer. In Expt. 1, 1-year old seedlings were treated for 194 days with three NO3:NH4 ratios at 25:75, 50:50, and 75:25 and two rates of N at 100 and 200 mg·L−1 in a factorial design. There was no interaction between the N rate and form on any growth parameters. Nitrogen form did not significantly affect shoot dry weight, root dry weight, root–to-shoot ratio, or the total dry weight. There was no significant difference between N rate of 100 and 200 mg·L−1 on root dry weight, root-to-shoot ratio, or the total dry weight. The shoot dry weight of Texas mountain laurel fertilized with 100 mg·L−1 was higher compared with that of the plants fertilized at 200 mg·L−1. The reduced shoot dry weight at N of 200 mg·L−1 was the result of the higher substrate salinity. In Expt. 2, seedlings were fertilized with five N rates (50, 100, 150, 200, and 250 mg·L−1) for 203 days. Plants watered with 150, 200, and 250 mg·L−1 were taller than those fertilized with 50 mg·L−1. The shoot height of plants watered with 100 mg·L−1 was only significantly different from 50 mg·L−1. For rapid growth of Texas mountain laurel, a N rate range of ≈150 mg·L−1 was recommended supplied with a combination of NO3-N and NH4-N in the ratios of 0.3 to 3.0.


Antioxidants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2024
Author(s):  
Fei Cheng ◽  
Min Gao ◽  
Junyang Lu ◽  
Yuan Huang ◽  
Zhilong Bie

Grafting with pumpkin rootstock could improve chilling tolerance in watermelon, and salicylic acid (SA) as a signal molecule is involved in regulating plant tolerance to chilling and other abiotic stresses. To clarify the mechanism in pumpkin rootstock-induced systemic acquired acclimation in grafted watermelon under chilling stress, we used self-grafted (Cl/Cl) and pumpkin rootstock-grafted (Cl/Cm) watermelon seedlings to study the changes in lipid peroxidation, photosystem II (PSII) activity and antioxidant metabolism, the spatio–temporal response of SA biosynthesis and H2O2 accumulation to chilling, and the role of H2O2 signal in SA-induced chilling tolerance in grafted watermelon. The results showed that pumpkin rootstock grafting promoted SA biosynthesis in the watermelon scions. Chilling induced hydrolysis of conjugated SA into free SA in the roots and accumulation of free SA in the leaves in Cl/Cm plants. Further, pumpkin rootstock grafting induced early response of antioxidant enzyme system in the roots and increased activities of ascorbate peroxidase and glutathione reductase in the leaves, thus maintaining cellular redox homeostasis. Exogenous SA improved while the inhibition of SA biosynthesis reduced chilling tolerance in Cl/Cl seedlings. The application of diphenyleneiodonium (DPI, inhibitor of NADPH oxidase) and dimethylthiourea (DMTU, H2O2 scavenger) decreased, while exogenous H2O2 improved the PSII activity in Cl/Cl plants under chilling stress. Additionally, the decrease of the net photosynthetic rate in DMTU- and DPI-pretreated Cl/Cl plants under chilling conditions could be alleviated by subsequent application of H2O2 but not SA. In conclusion, pumpkin rootstock grafting induces SA biosynthesis and redistribution in the leaves and roots and participates in the regulation of antioxidant metabolism probably through interaction with the H2O2 signal, thus improving chilling tolerance in watermelon.


2013 ◽  
Vol 5 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Mohammad SAYYARI ◽  
Fardin GHANBARI ◽  
Sajad FATAHI ◽  
Fatemeh BAVANDPOUR

Chilling temperatures lead to numerous physiological disturbances in the cells of chilling-sensitive plants and result in chilling injury and death of tropical and subtropical plants such as watermelon. In this study, the possibility of cold stress tolerance enhancing of watermelon seedling (Citrullus lanatus) by exogenous application of Salicylic acid (SA) was investigated. SA was applied through seed soaking or foliar spray at 0, 0.5, 1 and 1.5 mM concentration. After SA treatment, the seedlings were subjected to chilling 5 h/day at 4°C for 5 days. Statistical analysis showed significant effects of the application methods and SA concentrations on plant growth parameters, photosynthetic pigments, electrolyte leakage, proline and chilling injury index. SA application improved growth parameters and increased chlorophyll content of watermelon seedling subjected to chilling stress and provided significant protection against chilling stress compared to non-SA-treated seedlings. Although two SA application methods improved chilling stress tolerance, seed soaking method provided better protection compared to foliar spray method. SA ameliorated the injury caused by chilling stress via inhibiting proline accumulation and leaf electrolyte leakage. The highest cold tolerance was obtained with 0.5 mM SA application. Results indicate that SA could be used effectively to protect watermelon seedling from damaging effects of chilling stress at the early stages of growth.


1984 ◽  
Vol 14 (6) ◽  
pp. 850-854 ◽  
Author(s):  
S. B. Rood ◽  
G. Daicos ◽  
T. J. Blake

Weekly applications of 0.4 mg gibberellic acid (GA) in 8 μL 95% ethanol micropipetted onto shoots of rooted cuttings increased Populuseuramericana (Dode) Guinier height growth by 54% and shoot dry weight by 25% after 21 days. Total leaf area increased by 21% as a result of more rapid leaf production and slightly larger leaf sizes. Root growth was unaffected by GA treatment. The observed GA-induced acceleration resulted from an increase in relative growth rate while mean net assimilation rate was unaffected. Owing to a substantial increase in the number and size of leaves, the leaf area ratio, representing the ratio of photosynthesizing to respiring material, increased. While direct micropipette application of GA promoted height growth and primary shoot dry weight accumulation, GA application through either foliar spray or soil drench also promoted the growth of secondary shoots. Application of GA to shoots was more effective in promoting shoot growth than application through the roots. Direct GA application also promoted the growth of P. alba L. × P. grandidentata Michx. and P. × canescens (Ait.) Smith × P. alba × P. grandidentata. Thus, GA can be used for hastening early growth of these trees under winter greenhouse conditions.


2008 ◽  
Vol 34 (4) ◽  
pp. 338-341 ◽  
Author(s):  
Maria Angélica Guimarães Barbosa ◽  
Delson Laranjeira ◽  
Rildo Sartori Barbosa Coelho

Resistance induction through the use of chemical inducers often results in physiological costs to the plant. In this study, induced resistance in cotton plants was evaluated with regard to physiological costs in a cultivar susceptible to Colletotrichum gossypii var. cephalosporioides (CNPA GO 2002 - 7997). Plants were cultivated in substrates with two levels of nitrogen and received two applications of acibenzolar-S-methyl (ASM), jasmonic acid (JA) and Agro-Mos® (AM) disease resistance inducers. Plant height (H), internodal length (IL), shoot fresh weight (SFW), root fresh weight (RFW), shoot dry weight (SDW) and root dry weight (RDW) were evaluated. The activity of the phenylalanine ammonia lyase (PAL) and peroxidase (POX) was also determined. The plants treated with ASM presented high physiological costs with an accentuated reduction in H, SFW and SDW, whereas those treated with JA exhibited a significant increase in SDW, and did not significantly differ from H and IL. In the potting mix supplemented with nitrogen, all inducers differed from the control treatment regarding to internodal length, whereas only ASM and AM presented a significant difference between one another in the potting mix without the addition of nitrogen. Significant correlations (P=0.05) were found for most of the variables analyzed, with greater correlations observed between SFW and SDW (0.94); IL and H (0.74); SFW and H (0.70); and SDW and H (0.70). ASM induced the least amount of PAL activity, significantly differing from the remaining treatments. Greater POX activity was observed in ASM, which significantly differed from the control. AM and JA, however, presented lower activity than the control with regard to these enzymes, and it was not possible to confirm induction resistance in these two treatments.


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