scholarly journals Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil

2021 ◽  
Vol 37 (3) ◽  
Author(s):  
Fernanda Alvarenga Cardoso Medeiros ◽  
Job Alves de Souza Filho ◽  
José Ronaldo Barbosa ◽  
Lucas Edel Donato ◽  
Fabiano Borges Figueiredo ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Validation Study ◽  
Diagnostic Performance ◽  
Clinical Status ◽  
Control Measures ◽  
Canine Visceral Leishmaniasis ◽  
Reference Standard ◽  
Serum Samples ◽  
Transmission Cycle ◽  
Healthy Dogs

Abstract: Dogs are the main reservoirs in the domestic transmission cycle of visceral leishmaniasis, and the diagnosis is essential for the effectiveness of the control measures recommended by the Brazilian Ministry of Health. We assessed the diagnostic performance of the ELISA-Vetlisa/BIOCLIN prototype with serum samples from 200 dogs, in triplicate, including symptomatic, oligosymptomatic, asymptomatic, and healthy dogs, originated by two distinct panels (A and B) characterized by parasitological tests as the reference standard. In this study, the prototype kit showed a 99% sensitivity (95%CI: 94.5-100.0) and a 100% specificity (95%CI: 96.4-100.0). The sensitivity of the prototype kit did not vary significantly with the clinical status of the dogs. Considering the final result classification (positive or negative), agreement between the results of repeated tests was almost perfect (kappa = 0.99; 95%CI: 0.98-1.00). ELISA-Vetlisa/BIOCLIN is a promising option for the serological diagnosis of canine visceral leishmaniasis in Brazil.

scholarly journals Immunohaematological and rheological parameters in canine visceral leishmaniasis

2018 ◽  
Vol 27 (2) ◽  
pp. 211-217 ◽  
Author(s):  
José Nivaldo da Silva ◽  
Aron Carlos Cotrim ◽  
Luciana Auxiliadora Viebrantz da Conceição ◽  
Camila Moreira Ferreira Marins ◽  
Patricia Gelli Feres de Marchi ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Total Protein ◽  
Flow Curve ◽  
Rapid Test ◽  
Rheological Parameters ◽  
Control Group ◽  
Canine Visceral Leishmaniasis ◽  
Pcr Assay ◽  
Serum Samples ◽  
Healthy Dogs

Abstract The aim of this study was to find correlations between haematological and rheological parameters in canine visceral leishmaniasis (CLV). Enrolled dogs with CVL (n=31) had a confirmed diagnosis using the rapid test Dual Path Platform (TR DPP®) kit and Polymerase Chain Reaction (PCR) assay. A control group (n=31) comprised healthy dogs with negative results for the TR DPP® kit and PCR assay. Haematological parameters and total protein, albumin, globulin, and antibody were assessed. The rheological properties of the blood samples were also determined. Erythrocytes, haematocrit and platelet values of dogs in the CVL group were found to be lower than those of the control group. The total protein, globulin and IgG concentrations were higher in serum samples from the CVL group. The blood flow curve demonstrated increased shear rates in the CVL group. The viscosity of the blood from the infected animals was lower than in the healthy dogs. A positive correlation was found between erythrocyte numbers and blood viscosity. These data suggest that changes in the flow curve and viscosity of blood as well as the erythrocyte values may be a viable low-cost alternative for the monitoring of dogs with visceral leishmaniasis.

scholarly journals High accuracy of an ELISA test based in a flagella antigen of Leishmania in serodiagnosis of canine visceral leishmaniasis with potential to improve the control measures in Brazil – A Phase II study

2018 ◽  
Vol 12 (10) ◽  
pp. e0006871 ◽  
Author(s):  
Lairton Souza Borja ◽  
Lívia Brito Coelho ◽  
Matheus Silva de Jesus ◽  
Artur Trancoso Lopo de Queiroz ◽  
Paola Alejandra Fiorani Celedon ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Phase Ii ◽  
Phase Ii Study ◽  
High Accuracy ◽  
Elisa Test ◽  
Control Measures ◽  
Canine Visceral Leishmaniasis

scholarly journals Correction: High accuracy of an ELISA test based in a flagella antigen of Leishmania in serodiagnosis of canine visceral leishmaniasis with potential to improve the control measures in Brazil—A Phase II study

2020 ◽  
Vol 14 (5) ◽  
pp. e0008346
Author(s):  
Lairton Souza Borja ◽  
Lívia Brito Coelho ◽  
Matheus Silva de Jesus ◽  
Artur Trancoso Lopo de Queiroz ◽  
Paola Alejandra Fiorani Celedon ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Phase Ii ◽  
Phase Ii Study ◽  
High Accuracy ◽  
Elisa Test ◽  
Control Measures ◽  
Canine Visceral Leishmaniasis

scholarly journals Canine visceral leishmaniasis: Relationships between clinical status, humoral immune response, haematology and Lutzomyia (Lutzomyia) longipalpis infectivity

2007 ◽  
Vol 174 (3) ◽  
pp. 636-643 ◽  
Author(s):  
Adriane Pimenta da Costa-Val ◽  
Reginaldo Roris Cavalcanti ◽  
Nelder de Figueiredo Gontijo ◽  
Marilene Suzan Marques Michalick ◽  
Bruce Alexander ◽  
...  
Keyword(s):  
Immune Response ◽  
Visceral Leishmaniasis ◽  
Humoral Immune Response ◽  
Clinical Status ◽  
Lutzomyia Longipalpis ◽  
Canine Visceral Leishmaniasis ◽  
Humoral Immune

scholarly journals Performance of an indigenous β-mercaptoethanol-modified antigen in comparison with a commercial reference in direct agglutination test for detection of canine visceral leishmaniasis

2014 ◽  
Vol 63 (1) ◽  
pp. 106-110
Author(s):  
Saul José Semião-Santos ◽  
Laura Barral Veloso ◽  
Paulo Paes de Andrade ◽  
Marcia Almeida de Melo ◽  
Luis Miguel Lourenço Martins ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Leishmania Infantum ◽  
Leishmania Donovani ◽  
Agglutination Test ◽  
Canine Visceral Leishmaniasis ◽  
Direct Agglutination Test ◽  
Serum Samples

We compared the performance of a locally produced β-mercaptoethanol-modified promastigote antigen (β-ME-Ag) of an indigenous Leishmania infantum strain against that of a trypsinized Leishmania donovani reference (REF-Ag) in the direct agglutination test (DAT) for detection of canine visceral leishmaniasis (CVL). One hundred and fifty-one serum samples collected from dogs belonging to four groups with different conditions were included. At a DAT titre of 1 : 320, statistically determined as optimal cut-off value for β-ME-Ag, and 1 : 160 for REF-Ag, a sensitivity and a specificity of 100 % were estimated for β-ME-Ag in comparison with 96.6 % and 100 %, respectively, for REF-Ag. Overall, levels of agglutination titres recorded for the two antigens were highly concordant (Cohen’s κ = 0.879) in both the CVL and non-CVL groups. Based on current results, and ease experienced in processing the antigen and reading the test outcome, we recommend incorporation of β-ME-Ag in DAT for confirmation or exclusion of suspected CVL in dogs.

scholarly journals Spatial distribution of human and canine visceral leishmaniasis in Belo Horizonte, Minas Gerais State, Brasil, 1994-1997

2001 ◽  
Vol 17 (5) ◽  
pp. 1231-1239 ◽  
Author(s):  
Cláudia Di Lorenzo Oliveira ◽  
Renato Martins Assunção ◽  
Ilka Afonso Reis ◽  
Fernando Augusto Proietti
Keyword(s):  
Spatial Distribution ◽  
Visceral Leishmaniasis ◽  
Incidence Rates ◽  
Control Measures ◽  
Canine Visceral Leishmaniasis ◽  
Prevalence Rates ◽  
Smooth Maps ◽  
Human Visceral Leishmaniasis ◽  
Minas Gerais State ◽  
Belo Horizonte

In this paper, we present spatial analysis of the association between all incidents cases of human Visceral Leishmaniasis and seropositive dogs, from 1994 to 1997 in Belo Horizonte, a large Brazilian city. We geocoded 158 human cases and 11,048 seropositive dogs and compared canine prevalence rates with Human Bayesian Incidence rates in the same areas. We also used Knox's test to evaluate the hypothesis of space-time clustering of human cases in the period. Additionally, we used Kernel's maps for seropositive dogs distribution and located the human cases in the resulting smooth maps. We concluded that human and dog rates are correlated. Also, the Visceral Leishmaniasis in Belo Horizonte spread quickly, but apart from the rates' magnitude, it has kept the same spatial pattern through time. We believe it is possible to use this technique to choose areas to implement control measures against Visceral Leishmaniasis in a more efficient way.

scholarly journals Improving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests

2019 ◽  
Vol 114 ◽  
Author(s):  
Ana Izabel Passarella Teixeira ◽  
Debora Marcolino Silva ◽  
Tamires Vital ◽  
Nadjar Nitz ◽  
Bruna Caroline de Carvalho ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Canine Visceral Leishmaniasis ◽  
Reference Standard

scholarly journals Specific Serodiagnosis of Canine Visceral Leishmaniasis Using Leishmania Species Ribosomal Protein Extracts

2009 ◽  
Vol 16 (12) ◽  
pp. 1774-1780 ◽  
Author(s):  
Eduardo A. F. Coelho ◽  
Laura Ramírez ◽  
Mariana A. F. Costa ◽  
Vinicio T. S. Coelho ◽  
Vivian T. Martins ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Ribosomal Proteins ◽  
High Sensitivity ◽  
Leishmania Species ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Canine Visceral Leishmaniasis ◽  
Leishmania Chagasi ◽  
Serum Samples ◽  
Potential Tool

ABSTRACT In the present work, we have analyzed the antigenicity of Leishmania species ribosomal proteins (LRPs). To accomplish this, Leishmania infantum ribosomes were biochemically purified from promastigote cytosolic extracts, and their reactivities were analyzed by using the sera from dogs naturally infected with L. infantum. Since antibodies reacting against different ribosomal proteins were observed in all the serum samples obtained from dogs with symptomatic visceral leishmaniasis tested, we have analyzed the potential usefulness of the LRP extracts in the development of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of canine visceral leishmaniasis (CVL) in an area of Brazil where visceral leishmaniasis is endemic due to infection by Leishmania chagasi. A comparative ELISA with crude soluble Leishmania chagasi antigen (SLA) and L. infantum LRPs was performed. LRP- and SLA-based ELISAs gave similar sensitivities for the diagnosis of symptomatic CVL, but the LRP extract provided a very high sensitivity for the detection of oligosymptomatic and asymptomatic dogs. In addition, an LRP-based ELISA showed a higher specificity when the sera from dogs harboring other infections were included in the analysis. The LRP antigen displayed no cross-reactivity with sera from dogs that had any of the other diseases tested, notably, Chagas' disease. Our findings suggest that LRPs are a potential tool for the diagnosis of CVL and will be particularly useful for the diagnosis of asymptomatic CVL.

scholarly journals Cross-Reactivity Using Chimeric Trypanosoma cruzi Antigens: Diagnostic Performance in Settings Where Chagas Disease and American Cutaneous or Visceral Leishmaniasis Are Coendemic

2019 ◽  
Vol 57 (8) ◽  
Author(s):  
Ramona Tavares Daltro ◽  
Leonardo Maia Leony ◽  
Natália Erdens Maron Freitas ◽  
Ângelo Antônio Oliveira Silva ◽  
Emily Ferreira Santos ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Chagas Disease ◽  
Diagnostic Performance ◽  
Latent Class ◽  
Cross Reactivity ◽  
Chimeric Proteins ◽  
Serum Samples ◽  
Perfect Agreement ◽  
Content Type ◽  
Chronic Chagas Disease

ABSTRACT Chimeric T. cruzi antigens have been proposed as a diagnostic tool for chronic Chagas disease (CD) in both settings where Chagas disease is endemic and those where it is not endemic. Antibody response varies in accordance to each T. cruzi strain, presenting challenges to the use of antigens lacking demonstrated cross-reactivity with Leishmania spp. Our group expressed four chimeric proteins (IBMP-8.1, IBMP-8.2, IBMP-8.3, and IBMP-8.4) and previously assessed their diagnostic performance to determine cross-reactivity with Leishmania spp. Here, we validated our findings using serum samples from different Brazilian geographic areas reporting endemic Chagas disease, endemic visceral or American cutaneous leishmaniasis (ACL), or both. Overall, 829 serum samples were evaluated using commercial and IBMP enzyme-linked immunosorbent assays. Due to the absence of a reference assay to diagnosis CD, latent class analysis (LCA) was performed through the use of a statistical model. The incidence of cross-reactivity for ACL-positive samples varied from 0.35% (IBMP-8.3) to 0.70% (IBMP-8.1 and IBMP-8.2). Regarding visceral leishmaniasis (VL)-positive samples, the IBMP-8.2 and IBMP-8.3 antigens cross-reacted with six (3.49%) and with only one sample (0.58%), respectively. No cross-reactivity with either ACL or VL was observed for the IBMP-8.4 antigen. Similarly, no cross-reactions were found when VL-positive samples were assayed with IBMP-8.1. The agreement among the results obtained using IBMP antigens ranged from 97.3% for IBMP-8.2 and 99% for IBMP-8.1 and IBMP-8.3 to 100% for IBMP-8.4, demonstrating almost perfect agreement with LCA. Accordingly, in light of the negligible cross-reactivity with both ACL and VL, we suggest the use of IBMP antigens in regions where T. cruzi and Leishmania spp. are coendemic.

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