scholarly journals Antimicrobial Effect and Transdentinal Diffusion of New Intracanal Formulations Containing Nitrofurantoin or Doxycycline

2014 ◽  
Vol 25 (5) ◽  
pp. 425-429 ◽  
Author(s):  
Ana Rita Silva ◽  
Elizabete Brasil Santos ◽  
Shelon Cristina Souza Pinto ◽  
João Carlos Gomes ◽  
Irene Pina Vaz ◽  
...  
Keyword(s):  
Antimicrobial Effect ◽  
Root Surface ◽  
Positive Control ◽  
Diffusion Method ◽  
Dentinal Tubules ◽  
Agar Diffusion ◽  
Agar Diffusion Test ◽  
Agar Surface ◽  
Microbial Growth Inhibition

The aim of this study was to investigate in vitro the antimicrobial effect and diffusion against E. faecalis of new intracanal medications on the external root surface. The medications tested were a placebo gel (PC); the new formulations with either 3% nitrofurantoin (NIT) or 3% doxycycline hydrochloride (DX) and 2% chlorhexidine (CHX) gel as positive control. The new formulations were tested using the traditional agar diffusion test (ADT) and an adapted agar diffusion method (AADM), where the teeth were filled with the medications and left to diffuse on agar surface seeded with E. faecalis. In the ADT, the larger zones of microbial growth inhibition were seen in DX, followed by CHX and NIT. In the AADM test only DX and CHX showed antimicrobial effect. Statistically significant differences between groups were observed by the Kruskal-Wallis test (2=47.126; p<0.001). The new intracanal formulations with DX and NIT have demonstrated antimicrobial effect against E. faecalis, but only DX was able to diffuse through the dentinal tubules and exert antimicrobial effect outside the roots.

scholarly journals Antimicrobial Activity and Synergism of Lactoferrin and Lysozyme Against Cariogenic Microorganisms

2014 ◽  
Vol 25 (2) ◽  
pp. 165-169 ◽  
Author(s):  
Flaviana Bombarda de Andrade ◽  
Jair Caetano de Oliveira ◽  
Marjorie Takei Yoshie ◽  
Bruno Martini Guimarães ◽  
Rafael Braga Gonçalves ◽  
...  
Keyword(s):  
Inhibitory Concentration ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Inhibitory Effects ◽  
Broth Macrodilution ◽  
Agar Surface ◽  
In Vitro Effects ◽  
Carious Process ◽  
Microbial Inocula

The present study evaluated the antimicrobial in vitro effects of the salivary proteins lactoferrin and lysozyme on microorganisms involved in the carious process, obtaining their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Streptococcus mutans (ATCC 25175) and Lactobacillus casei (ATCC 7469) were submitted to broth macrodilution of lysozyme at 80 mg/mL and lactoferrin at 200 mg/mL. The tubes were read in a spectrophotometer after they had been incubated at 37 °C for 18 h, in a carbon dioxide chamber, in order to read the MIC. A new subculture was carried on agar plates to obtain the MBC. The agar diffusion method was also tested, using BHI agar with 100 µL of the standardized microbial inocula. Filter-paper disks soaked in 10 µL of the solutions lactoferrin (200 µg/mL) and lysozyme (80 µg/mL) were placed on the agar surface. Inhibition halos were not observed on the plates, showing the absence of the antimicrobial effects of these proteins in this method. The bactericidal and bacteriostatic effects of lysozyme on L. casei were 50.3 mg/mL and 43.1 mg/mL respectively. The bactericidal and bacteriostatic effects on S. mutans were 68.5 mg/mL and 58.7 mg/mL. Lactoferrin did not induce any inhibitory effects on any microorganism, even in the concentration of 200 mg/mL. There was not a synergic antimicrobial effect of proteins, when they were tested together, even in the concentration of 42.8 mg/mL of lysozyme and 114 mg/mL of lactoferrin (the highest values evaluated). S. mutans and L. casei were only inhibited by lysozyme, not affected by lactoferrin and by the synergic use of both proteins.

scholarly journals Antibacterial Activity of Leukocyte- and Platelet-Rich Plasma: AnIn VitroStudy

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Agata Cieślik-Bielecka ◽  
Tadeusz Bold ◽  
Grzegorz Ziółkowski ◽  
Marcin Pierchała ◽  
Aleksandra Królikowska ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Enterococcus Faecalis ◽  
Platelet Rich Plasma ◽  
Antibacterial Properties ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Bovine Thrombin ◽  
Zones Of Inhibition

The aim of the study was to investigate the leukocyte- and platelet-rich plasma (L-PRP) antimicrobial activity. The studied sample comprised 20 healthy males. The L-PRP gel, liquid L-PRP, and thrombin samples were testedin vitrofor their antibacterial properties against selected bacterial strains using the Kirby-Bauer disc diffusion method. Two types of thrombin were used (autologous and bovine). Zones of inhibition produced by L-PRP ranged between 6 and 18 mm in diameter. L-PRP inhibited the growth ofStaphylococcus aureus(MRSA and MSSA strains) and was also active againstEnterococcus faecalisandPseudomonas aeruginosa. There was no activity againstEscherichia coliandKlebsiella pneumoniae. The statistically significant increase of L-PRP antimicrobial effect was noted with the use of major volume of thrombin as an activator. Additionally, in groups where a bovine thrombin mixture was added to L-PRP the zones of inhibition concerning MRSA,Enterococcus faecalis, andPseudomonas aeruginosawere larger than in the groups with autologous thrombin. Based on the conducted studies, it can be determined that L-PRP can evokein vitroantimicrobial effects and might be used to treat selected infections in the clinical field. The major volume of thrombin as an activator increases the strength of the L-PRP antimicrobial effect.

In vitro antimicrobial effect of RC-Prep within dentinal tubules

1999 ◽  
Vol 25 (12) ◽  
pp. 782-785 ◽  
Author(s):  
Ilana Heling ◽  
Eldad Irani ◽  
Sunil Karni ◽  
Doron Steinberg
Keyword(s):  
Antimicrobial Effect ◽  
Dentinal Tubules

scholarly journals Antimicrobial effect of 2% sodium hypochlorite and 2% chlorhexidine tested by different methods

2003 ◽  
Vol 14 (1) ◽  
pp. 58-62 ◽  
Author(s):  
Carlos Estrela ◽  
Rosane Galhardo Ribeiro ◽  
Cyntia R.A. Estrela ◽  
Jesus Djalma Pécora ◽  
Manoel Damião Sousa-Neto
Keyword(s):  
Sodium Hypochlorite ◽  
Culture Medium ◽  
Agar Plate ◽  
Antimicrobial Effect ◽  
Control Group ◽  
Diffusion Test ◽  
Exposure Test ◽  
Agar Diffusion ◽  
Direct Exposure ◽  
Agar Diffusion Test

The objective of this study was to analyze the antimicrobial effect of 2% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX) by agar diffusion test and by direct exposure test. Five microorganisms: Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans, and one mixture of these were used. These strains were inoculated in brain heart infusion (BHI) and incubated at 37ºC for 24 h. For the agar diffusion test (ADT), 18 Petri plates with 20 ml of BHI agar were inoculated with 0.1 ml of the microbial suspensions, using sterile swabs that were spread on the medium, obtaining growth in junction. Fifty-four paper disks (9 mm in diameter) were immersed in the experimental solutions for 1 min. Subsequently, three papers disks containing one of the substances were placed on the BHI agar surface in each agar plate. The plates were maintained for 1 h at room temperature, and then incubated at 37ºC for 48 h. The diameter of microbial inhibition was measured around the papers disks containing the substances. For the direct exposure test, 162 #50 sterile absorbent paper points were immersed in the experimental suspensions for 5 min, and were then placed on Petri plates and covered with one of the irrigant solutions, or with sterile distilled water (control group). After intervals of 5, 10 and 30 min, the paper points were removed from contact with the solutions and individually immersed in 7 ml of Letheen Broth, followed by incubation at 37ºC for 48 h. Microbial growth was evaluated by turbidity of the culture medium. A 0.1 ml inoculum obtained from the Letheen Broth was transferred to 7 ml of BHI, and incubated at 37ºC for 48 h. Bacterial growth was again evaluated by turbidity of the culture medium. Gram stain of BHI cultures was used for verification of contamination and growth was determined by macroscopic and microscopic examination. The best performance of antimicrobial effectiveness of NaOCl was observed in the direct exposure test, and of CHX was observed in the agar diffusion test. The magnitude of antimicrobial effect was influenced by the experimental methods, biological indicators and exposure time.

scholarly journals Evaluation of the antimicrobial effect of calcium hydroxide combined to different vehicles

2016 ◽  
Vol 31 (2) ◽  
pp. 89
Author(s):  
Tatiana Dantas Piana ◽  
Maria de Fátima Malvar Gesteira ◽  
Erica Dos Santos Carvalho ◽  
Josilene Borges Torres Lima Matos ◽  
Monica Franca ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Antimicrobial Effect ◽  
Antimicrobial Action ◽  
Diffusion Method ◽  
Agar Diffusion ◽  
Inhibition Zones ◽  
Agar Diffusion Method

Objective: To evaluate the potentiating effect of different substances in antimicrobial action of calcium hydroxide.Methods: The agar diffusion method, was used with well technique, to analyze seven substances associated to calcium hydroxide to make some pastes, they are: chlorexidine, saline, anesthetic, malvatricin, propolis, hypochlorite, paramonochlorophenol.Results: The pastes with malvatricin and paramonochlorophenol presented greater inhibition zones against Enterococcus faecalis.Conclusion: There was potentialization in antimicrobial effect of calcium hydroxide when associated to paramonochlorophenol and to malvatricin especially against Enterococcus faecalis. Low increase of the antimicrobial capacity was observed when using chlorexidine as vehicle to calcium hydroxine paste.

scholarly journals Daya Antibakteri Ekstrak Etanol Ketepeng Cina (Senna alata) terhadap Staphylococcus aureus dan Escherichia coli secara In Vitro

2012 ◽  
Vol 13 (1) ◽  
pp. 63
Author(s):  
Taswin Yacob ◽  
Rita Endriani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Diffusion Method ◽  
Randomized Design ◽  
Senna Alata ◽  
Treatment Of Infection ◽  
Range Test

The benefit and efficacy of ketepeng cina (Senna alata) in the treatment of infection has shown that have antibacterialactivity, inhibiting and killing bacteria that cause infection. The objective of this study was evaluate the antibacterialactivity of ketepeng cina against Staphylococcus aureus dan Escherichia coli in vitro. This study was a laboratoryexperimental research which use completely randomized design with diffusion method. Ethanol extract of Sennaalata leaves devided into 4 doses, i.c. 100, 50, 25 and 12.5. Amoxiclave were used as positive control and aquadestnegative control. The data were analyzed by Analysis of Varian continued with Duncan’s Multiple Range Test. Theresult of this study showed that antimicrobial activity of ethanol extract Senna alata leaves inhibited the growth ofStaphylococcus aureus, but not Escherichia coli. The optimum effect was showed given by the concentration 100at 17.7 mm.

scholarly journals Kefiran Ekstraktının Bazı Bitki Patojeni Bakterilerine Karşı Antimikrobiyal Etkisinin Değerlendirilmesi

2020 ◽  
Vol 8 (4) ◽  
pp. 889-894
Author(s):  
Bilgin Taşkın
Keyword(s):  
Antibacterial Effect ◽  
Antimicrobial Effect ◽  
Acetic Acid Bacteria ◽  
Fermented Milk ◽  
Diffusion Method ◽  
Milk Product ◽  
Bacterial Strains ◽  
Private Households ◽  
Different Temperatures

Kefir; is a fermented milk product which is produced by granules containing a wide variety of microorganisms such as lactic acid bacteria, acetic acid bacteria and yeasts. It is traditionally consumed in many countries. It has been shown in many studies that the polysaccharide structure surrounding the granules which is composed mainly of kefiran molecule has antimicrobial effect against various pathogens as well as many health promoting effects. In this study, 24 h fermented kefir was used with two types of kefir granules for production of kefiran extract. One of them is being sold commercially and the other was collected from private households in a different region of Turkey. Kefiran extraction was carried out from matured kefir granules using three different temperatures, 80°C, 90°C and 100°C. Also, the protein contents of the extracted solutions were determined by Bradford method. Protein content of the extract solutions obtained were measured as 0.001 g/ml. The antibacterial effect of 0.05, 0.1, 1 and 2 mg of this extract against several plant pathogenic bacterial strains belonging to genus Pseudomonas, Xanthomonas, Erwinia ve Clavibacter was investigated in vitro for the first time. For this purpose, two methods, disc diffusion method and spreading method were used. The AN and SD kefir supernatants used as the positive controls in the experiments showed an average of 13-17 mm and 10-14 mm inhibition zones on the isolates, respectively, but the antibacterial effect of kefiran extracts was not observed.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK PERASAN DAUN MENGKUDU (Morinda citrifolia L.) TERHADAP Escherichia coli SECARA IN VITRO

2020 ◽  
Vol 2 (1) ◽  
pp. 26-34
Author(s):  
Margareta Retno Priamsari ◽  
Agastia Cicilia Wibowo
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Drying Shrinkage ◽  
Positive Control ◽  
Negative Control ◽  
Morinda Citrifolia ◽  
Diffusion Method ◽  
Juice Extraction ◽  
Water Inhibition

Noni juice can inhibit the growth of Escherichia coli bacteria. Noni juice extraction needs concentration to extract so that the preparation is more stable in the storage process. The purpose of this study was to determine the antibacterial activity and the amount of the minimum inhibitory concentration of noni juice extract from E. coli bacteria in vitro. This type of experimental research with a completely randomized one-way design. The extract was obtained by concentrating the Noni leaf extract. Extract quality control parameters include organoleptic, yield, drying shrinkage, and qualitative tests of flavonoid and anthraquinone compounds. Antibacterial activity test using the disc diffusion method with an extract concentration of 1.56%; 3.12%; 6.25%; 12.5%; and 25% with 3 replications. Positive control of amoxicillin and negative control of distilled water. Inhibition is known from the zone formed around the paper disc. The data obtained were statistically analyzed using Kruskall Wallis followed by Mann Whitney with a 95% confidence level. The results showed that the variation in the concentration of the noni juice extract had a significant effect (p <0.05). The biggest inhibitory zone was seen at 25% concentration of 10.16 mm and included in the strong category. The minimum inhibitory power was produced at a concentration of 3.12% at 2.50 mm with a weak treatment category.

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