AKTIVITAS ANTIBAKTERI EKSTRAK PERASAN DAUN MENGKUDU (Morinda citrifolia L.) TERHADAP Escherichia coli SECARA IN VITRO

Noni juice can inhibit the growth of Escherichia coli bacteria. Noni juice extraction needs concentration to extract so that the preparation is more stable in the storage process. The purpose of this study was to determine the antibacterial activity and the amount of the minimum inhibitory concentration of noni juice extract from E. coli bacteria in vitro. This type of experimental research with a completely randomized one-way design. The extract was obtained by concentrating the Noni leaf extract. Extract quality control parameters include organoleptic, yield, drying shrinkage, and qualitative tests of flavonoid and anthraquinone compounds. Antibacterial activity test using the disc diffusion method with an extract concentration of 1.56%; 3.12%; 6.25%; 12.5%; and 25% with 3 replications. Positive control of amoxicillin and negative control of distilled water. Inhibition is known from the zone formed around the paper disc. The data obtained were statistically analyzed using Kruskall Wallis followed by Mann Whitney with a 95% confidence level. The results showed that the variation in the concentration of the noni juice extract had a significant effect (p <0.05). The biggest inhibitory zone was seen at 25% concentration of 10.16 mm and included in the strong category. The minimum inhibitory power was produced at a concentration of 3.12% at 2.50 mm with a weak treatment category.

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AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI KARANG LUNAK Sinularia SP. DI TELUK MANADO

PHARMACON ◽

10.35799/pha.8.2019.29244 ◽

2019 ◽

Vol 8 (1) ◽

pp. 114

Author(s):

Lyone Katiandagho ◽

Defny S Wewengkang ◽

Sri Sudewi

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Soft Coral ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Chloroform Fraction ◽

Water Fraction

ABSTRACT Soft Coral Sinularia sp.has been shown to have antibacterial activity. This research aims to determine the antibacterial activity of marine organisms obtained from Manado bay. The method in this study is the diffusion method with positive control chloramphenicol and negative control of methanol in Escerichia coli and Sthaphylococcus aureus. The result showed that extracts, chloroform fraction and methanol-water fraction were obtained inhibiting category of Sthapylococcus aureus bacteria, but in the chloroform fraction and ethanol extract can inhibit Escerichia coli bacteria categorized as strong. With it can be conlueded that the extract and fraction of soft Sinularia sp. have bioactive compounds with a broth spectrum of antibacterial activity.Keywords: Sinularia sp., Chloramphenicol, Escerichia coli, Sthaphylococcus aureus, and antibacterial. ABSTRAK Karang lunak Sinularia sp. telah terbukti memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri pada organisme biota laut yang diperoleh dari teluk Manado. Metode dalam penelitian ini adalah metode difusi agar dengan kontrol positif kloramfenikol dan kontrol negatit metanol pada Escherichia coli dan Staphylococcus aureus. Hasil penelitian menunjukkan bahwa ekstrak, fraksi kloroform, dan fraksi metanol-air dapat menghambat bakteri Staphylococcus aereus dikategorikan sedang, namun pada fraksi kloroform dan ekstrak etanol dapat menghambat bakteri Escerichia coli dikategorikan kuat. Dengan demikian dapat disimpulkan bahwa ekstrak dan fraksi karang lunak Sinularia sp.memiliki senyawa bioaktif dengan spektrum yang luas terhadap aktivitas antibakteri.Kata kunci: Sinularia sp, Kloramfenikol, Escherichia coli, Staphylococcus aureus, dan antibakteri

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SKRINING FITOKIMIA DAN AKTIVITAS ANTIBAKTERI in vitro EKSTRAK HEKSANA-PETROLEUM ETER Artemisia cina Berg. ex Poljakov

Agric ◽

10.24246/agric.2015.v27.i1.p30-37 ◽

2016 ◽

Vol 27 (1) ◽

pp. 30

Author(s):

Elizabeth B.E Kristiani ◽

Sri Kasmiyati ◽

Maria Marina Herawati

Keyword(s):

Antibacterial Activity ◽

Petroleum Ether ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Phytochemical Analysis ◽

E Coli ◽

Randomized Design ◽

Antibactrial Activity

The objectives of this reserch were to determine the antibacterial activity of hexane-petroleum ether (1 : 1 v/v) extract of Artemisia cinaBerg. ex Poljakov on Escherichia coli and Staphylococcus aureus and its compounds. The design of the research was using completely Randomized Design in five concentration of extract that were 0, 50, 75, 100, 150, dan 200 mg/ml with four repliceted. Ethanol 60% was used as negative control and tetracycline as positive control. There were three steps of research. First step was extracted the plant using soxhlet method with hexane-petroleum ether (1:1 v/v). Second step was determined the antibacterial activity of hexane-petroleum ether (1:1 v/v) extract in various concentration of that extracts on E. Coli and S. aureus using agar diffusion method. Analysis of Variance (ANOVA) and was used to determined the significan different of diameter of inhibition between the treatments. Thirdsteps was phytochemical analysis of extract. The highest antibactrial activity on E. Coli was at 100 mg/ml whereas on S. Aureus at 150 mg/ml. That extract was contained flavonoid, alkaloid, essential oils, saponin, sterol, tritepene, hydrolized tannin, and coumarin.

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The Antibacterial Activity of Thermoactinomyces sp. (H24) Extract Against Escherichia coli and Staphylococcus aureus

Indonesian Journal of Medical Laboratory Science and Technology ◽

10.33086/ijmlst.v3i1.1700 ◽

2021 ◽

Vol 3 (1) ◽

pp. 56-63

Author(s):

Julia Nanda Puspita ◽

Rikhsan Kurniatuhadi ◽

Rahmawati Rahmawati

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bioactive Compounds ◽

Bioactive Compound ◽

Inhibitory Effect ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

And Staphylococcus Aureus

Bacteria of the genus Thermoactinomyces have the ability to produce antibacterial bioactive compounds. This bioactive compound can be used for combating diarrheal agents such as Escherichia coli and Staphylococcus aureus. This study aims to determine the antibacterial activity of the metabolite extract from Thermoactinomyces sp. (H24) against Escherichia coli and Staphylococcus aureus. Methanol was used as a solvent for the extraction of bacterial bioactive compounds. Antibacterial activity was analyzed by the diffusion method with several extract concentrations (0.75 mL, 1.5 mL, 2.25 mL, and 3 mL), 10% DMSO as the negative control, and ciprofloxacin as the positive control. Our result shows that Termoactinomyces sp. (H24) extract has an inhibitory effect on the growth of E. coli and S. aureus with an effective concentration of 2.25 mL (inhibition strength: very strong).

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK BIJI BUAH DUKU Lansium domesticum TERHADAP BAKTERI STAPHYLOCOCUS AUREUS DAN ESCHERICHIA COLI

PHARMACON ◽

10.35799/pha.10.2021.34030 ◽

2021 ◽

Vol 10 (2) ◽

pp. 818

Author(s):

Albrita Pehino ◽

Fatimawali Fatimawali ◽

Elly J. Suoth

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Nutritional Value ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Activity Test ◽

Solution Studies ◽

Lansium Domesticum

ABSTRACT Duku (Lansium domesticum) provides many benefits for the community. Apart from the fruit which has high nutritional value, duku is believed by the community to have benefits. The study aims to learn about the antibacterial extract of my seed against the staphylococus aureus and escherichia coli. This study used the meseration method with ethanol as a solvent. The antibacterial activity test used the well diffusion method with a concentration of 10%, 20%, 30%, 40%. The positive control used was Ciprofloxacin and the negative control used was the CMC solution. Studies show that the suppression of extract is 10%, 20%, 30%, and 40% staphylococus aureus, the average drip zone is 11.3mm, 13.3mm, 13.6mm, 11,3mm, and escherichia coli, on average, 10 mm, 9,3mm, 10,3mm, 12,6 mm. The results suggest that the extract of the duku fruit has antibacterial activity against the growth of the staphylococcus aureus and escherichia coli. Keywords: Duku seed, Antibacterial, Antibacterial activity ABSTRAK Duku (Lansium domesticum) banyak memberikan manfaat bagi masyarakat. Selain buahnya yang mempunyai nilai gizi tinggi, duku dipercaya masyarakat memiliki manfaat untuk kesehatan. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak biji duku terhadap bakteri staphylococus aureus dan Escherichia coli. Penelitian ini menggunakan metode maserasi dengan pelarut etanol. Uji aktivitas antibakteri menggunakan metode difusi sumuran dengan konsentrasi 10%, 20%, 30%, 40%. Kontrol positif yang digunakan adalah Ciprofloxacin dan kontrol negatif yang digunakan adalah larutan CMC. Hasil penelitian menunjukan bahwa daya hambat ekstrak dengan konsentrasi 10%, 20%, 30%, dan 40% Staphylococcus aureus rata-rata zona beningnya 11,3mm, 13,3mm, 13,6mm, 11,3mm, dan Escherichia coli rata-rata zona beningnya 10 mm, 9,3mm, 10,3mm, 12,6 mm. Dari hasil yang didapatkan disimpulkan bahwa ekstrak biji buah duku memiliki aktivitas antibakteri terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Kata Kunci:Biji buah duku, Antibakteri, Aktivitas antibakteri

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UJI DAYA HAMBAT EKSTRAK ETANOL DAUN KACAPIRING (Gardenia augusta) TERHADAP BAKTERI Staphylococcus aureus, Escherichia coli DAN Salmonella thypi

PHARMACON ◽

10.35799/pha.9.2020.29281 ◽

2020 ◽

Vol 9 (2) ◽

pp. 268

Author(s):

Serly D. S. Toding ◽

Herny E. I. Simbala ◽

Deby A. Mpila

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Medicinal Plants ◽

Leaf Extract ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Inhibitory Power ◽

Paper Disc

ABSTRACT One of the potential medicinal plants as traditional medicine is gardenia plant (Gardenia augusta). Gardenia leaf extract contains alkaloid compounds, flavonoids, tannins, saponins and triterpenoids, which are known to have antibacterial abilities..The aim of this research was to determine the antibacterial activity of gardenia leaf extract on the growth of Staphylococcus aureus, Escherichia coli and Salmonella thypi. The examination on the inhibitory power using the Kirby-Bauer method. The Kirby-Bauer method is referring to the diffusion method using paper disc with five treatments, namely the extract of gardenia leaves with a substance concentration of 20%, 40%, and 60% as well positive control (Ciprofloxacin) and negative control (aquades). Based on the research results obtained, it can be concluded that gardenia leaf extract can inhibit growth of Staphylococcus aureus, Escherichia coli and Salmonella thypi at a concentration of 20%, 40% and 60% and classified as strong and medium category. Keywords : Gardenia augusta, antibacterial, of Staphylococcus aureus, Escherichia coli, Salmonella thypiABSTRAK Salah satu tanaman obat dengan potensi sebagai obat tradisional adalah tanaman kacapiring (Garddenia augusta). Daun kacapiring mengandung senyawa alkaloid, flavonoid, tanin, saponin dan triterpenoid, yang berpotensi sebagai antibakteri sehingga digunakan dalam penelitian ini.Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak daun kacapiring terhadap pertumbuhan Staphylococcus aureus, Escherichia coli dan Salmonella thypi. Pengujian daya hambat menggunakan metode Kirby-Bauer, yaitu metode difusi dengan cakram kertas dengan 5 perlakuan yaitu ekstrak daun kacapiring dengan konsentrasi 20%, 40%, dan 60% serta kontrol positif (ciprofloxacin) dan kontrol negatif (akuades). Berdasarkan hasil penelitian yang diperoleh, dapat disimpulkan bahwa ekstrak daun kacapiring dapat menghambat pertumbuhan bakteri Staphylococcus aureus, Escherichia coli dan Salmonella thypi pada konsentrasi 20%, 40% dan 60% dan tergolong dalam kategori kuat dan sedang. Kata Kunci : Kacapiring, Antibakteri, Staphylococcus aureus, Escherichia coli, Salmonella thypi

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Pengujian Aktivitas Antibakteri Hidrogel Ekstrak Etanol Daun Tagetes erecta L

Jurnal MIPA ◽

10.35799/jmuo.8.3.2019.25582 ◽

2019 ◽

Vol 8 (3) ◽

pp. 96

Author(s):

Hosea Jaya Edy ◽

Marchaban Marchaban ◽

Subagus Wahyuono ◽

Agung Endro Nugroho

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethanolic Extract ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Tagetes Erecta ◽

Tagetes Erecta L

Kandungan kimia flavonoid dan fenolik dalam ekstrak daun T.erecta diketahui memiliki aktivitas antibakteri yang baik. Pengunaan ekstrak daun T.erecta sebagai zat aktif sediaan hidrogel akan sangat mempermudah pemanfaatan dalam proses terapi oleh masyarakat. Tujuan dalam penelitian ini adalah melihat kemampuan aktivitas antibakteri sediaan hidrogel dengan zat aktif ekstrak daun T.erecta. Dalam penelitian ini digunakan 6 variasi konsentrasi ekstrak yang terkandung pada sediaan hidrogel yaitu 1.5; 2.0; 2.5; 3.0; 3.5; dan 4.0 %. Pengujian aktivitas antibakteri menggunakan metode difusi sumuran terhadap bakteri Staphylococcus aureus dan Escherichia coli. Aktivitas antibakteri terbesar ditunjukkan oleh sediaan hidrogel dengan konsentrasi ekstrak daun T.erecta 2.5%. Diameter zona hambat yang terbentuk terhadap pertumbuhan E.coli adalah 15.33 mm sedangkan diameter zona hambat terhadap S.aureus adalah 17.87 mm. Untuk kontrol positif digunakan bioplacenton® dan untuk kontrol negatif digunakan basis hidrogel tanpa kandungan ekstrak T.erectaFlavonoid and phenolic on ethanolic extract of T.erecta L has been antibacterial activity. The aim of this research was to determinate of antibacterial from hydrogel with ethanolic extract of T.erecta L. This research was begun making hydrogels with six variations of ethanolic extract of T.erecta L were 1.5; ; 2.0; 2.5; 3.0; 3.5; and 4.0 %. Antibacterial activity used hole diffusion method against Staphylococcus aureus and Escherichia coli. Hydrogel containing 2.5 % ethanolic extract of T.erecta L was shown the highest antibacterial activity. The diameters of the inhibition zone against E.coli was 15.33 mm and against S.aureus was 17.87 mm. Positive control used bioplacenton® and negative control was hydrogel without ethanolic extract of T.erecta content

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Antibacterial Activity Test of the C-4-methoxyphenylcalix[4]resorcinarene Compound Modified by Hexadecyltrimethylammonium-Bromide against Staphylococcus aureus and Escherichia coli Bacteria

JKPK (Jurnal Kimia dan Pendidikan Kimia) ◽

10.20961/jkpk.v3i3.22742 ◽

2018 ◽

Vol 3 (3) ◽

pp. 201 ◽

Cited By ~ 1

Author(s):

Suryadi Budi Utomo ◽

Mita Fujiyanti ◽

Warih Puji Lestari ◽

Sri Mulyani

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Hexadecyltrimethylammonium Bromide ◽

E Coli ◽

Escherichia Coli Bacteria

This study aims to determine the antibacterial activity of C-4-methoxyphenylcalix-[4]resorcinarene modified by Hexadecyltrimethylammonium-Bromide (Resorcinarene-HDTMA-Br) against Staphylococcus aureus and Escherichia coli bacteria. The study was conducted by testing the inhibition zone diameter with paper disc diffusion method. Variations in the concentration of the sample used were 10%; 15%; 20%; 25%; and 30% b/v. In this study, Dimethyl sulfoxide (DMSO) (sampel solvent) was used as a negative control and Chloramphenicol as a positive control. It was found that Resorsinarene-HDTMA-Br had higher antibacterial activity than the unmodified C-4-methoxyphenylcalix[4]resorcinarene (CMFKR) against the S. aureus bacteria. Both CMFKR and Resorcinarene-HDTMA-Br are not active against E. coli.

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Phytocimia Test And Antibacterial Bioactivity Of Extracts from Stem Bark of Artocarpus integer (Thunb.) Merr

Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) ◽

10.20956/ica.v12i2.7214 ◽

2019 ◽

Vol 12 (2) ◽

pp. 84

Author(s):

Liska Hamzah

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Stem Bark ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Chloroform Fraction ◽

Agar Media ◽

And Staphylococcus Aureus

This research aims to identify the compound group and determine the antibacterial activity of n-hexane, chloroform, and methanol fractions of Artocarpus integer (Thunb.) Merr stem bark. All fractions contain groups of terpenoid and steroid compounds. Antibacterial activity was detemined using agar diffusion method with MHA (Muller Hinton Agar) media on Escherichia coli (gram negative) and Staphylococcus aureus (gram positive). Ciprofloxacin was used as a positive control and methanol as a negative control. The three fractions were able to inhibit the growth of Escherichia coli and Staphylococcus aureus. The chloroform fraction showed the highest activity after 48 hours incubation with inhibition zones of 10.8 and 10.4 mm respectively.

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Daya Antibakteri Ekstrak Etanol Ketepeng Cina (Senna alata) terhadap Staphylococcus aureus dan Escherichia coli secara In Vitro

Jurnal Natur Indonesia ◽

10.31258/jnat.13.1.63-66 ◽

2012 ◽

Vol 13 (1) ◽

pp. 63

Author(s):

Taswin Yacob ◽

Rita Endriani

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Ethanol Extract ◽

Positive Control ◽

Diffusion Method ◽

Randomized Design ◽

Senna Alata ◽

Treatment Of Infection ◽

Range Test

The benefit and efficacy of ketepeng cina (Senna alata) in the treatment of infection has shown that have antibacterialactivity, inhibiting and killing bacteria that cause infection. The objective of this study was evaluate the antibacterialactivity of ketepeng cina against Staphylococcus aureus dan Escherichia coli in vitro. This study was a laboratoryexperimental research which use completely randomized design with diffusion method. Ethanol extract of Sennaalata leaves devided into 4 doses, i.c. 100, 50, 25 and 12.5. Amoxiclave were used as positive control and aquadestnegative control. The data were analyzed by Analysis of Varian continued with Duncan’s Multiple Range Test. Theresult of this study showed that antimicrobial activity of ethanol extract Senna alata leaves inhibited the growth ofStaphylococcus aureus, but not Escherichia coli. The optimum effect was showed given by the concentration 100at 17.7 mm.

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AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI KAPULAGA JAWA (Amomum compactum Soland. ex Maton) TERHADAP Streptococcus pyogenes

EKOLOGIA ◽

10.33751/ekologia.v20i1.1985 ◽

2020 ◽

Vol 20 (1) ◽

pp. 31-39

Author(s):

Oom Komala ◽

. Ismanto ◽

Muhammad Alan Maulana

Keyword(s):

Antibacterial Activity ◽

Streptococcus Pyogenes ◽

Pathogenic Bacteria ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Dilution Method ◽

Natural Treatment ◽

Amomum Compactum

Streptococcus pyogenes is one of the pathogenic bacteria that causes pharyngitis. Natural treatment to overcome these problems is to use cardamom seeds. The purpose of this study was to test the antibacterial activity, determine the concentration of inhibitory zone and phytochemical compounds from ethanol 96% extract of Java cardamom seeds (Amomum compactum Soland. Ex Maton) against Streptococcus pyogenes. The method is used solid dilution and paper disc diffusion method. The solid dilution method is used for the Minimum Inhibitory Concentration (MIC) test with a concentration of 1.25%, 2.5%, 5% and 7.5% while the paper diffusion method is used for the Inhibition zone Diameter (IZD) using five treatments namely three concentrations of ethanol 96% extract of Java cardamom seeds (7.5%, 10% and 12%), one positive control of amoxicillin 0.01 mg/mL and one negative control of sterile distilled water. IZD data were analyzed using ANOVA with a confidence level of 95% and α = 0.05 and Duncan's further tests to determine differences between treatments. The results showed that the MIC was at a concentration of 7.5% while for the IZD test which had the highest activity there was a concentration of 12% with an average inhibition diameter of 12.03 ± 0.14 mm. In addition, ethanol 96% extract of Java cardamom seeds contain alkaloids, flavonoids, terpenoids and tannins which function in antibacterial activity.

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