Acute inflammation and hematological response in Nile tilapia fed supplemented diet with natural extracts of propolis and Aloe barbadensis

This study evaluated the acute inflammatory response induced by carrageenin in the swim bladder of Nile tilapia supplemented with the mixture of natural extracts of propolis and Aloe barbadensis (1:1) at a concentration of 0.5%, 1% and 2% in diet during 15 days. Thirty-six fish were distributed into four treatments with three replicates: fish supplemented with 0.5% of admix of extracts of propolis and Aloe (1:1) injected with 500 µg carrageenin; fish supplemented with 1% of admix of extracts of propolis and Aloe (1:1) injected with 500 µg carrageenin; fish supplemented with 2% of admix of extracts of propolis and Aloe (1:1), injected with 500 µg carrageenin and unsupplemented fish injected with 500 µg carrageenin. Six hours after injection, samples of blood and exudate from the swim bladder of fish were collected. It was observed an increase in the leukocyte count in the swim bladder exudate of fish supplemented with extracts of propolis and Aloe injected with carrageenin. The most frequent cells were macrophages followed by granular leukocytes, thrombocytes and lymphocytes. Supplementation with propolis and Aloe to 0.5% caused a significant increase in the number of cells on the inflammatory focus mainly macrophages, cells responsible for the phagocytic activity in tissues, agent of innate fish immune response.

Download Full-text

Leukocyte phagocytosis and lysozyme activity in Nile tilapia fed supplemented diet with natural extracts of propolis and Aloe barbadensis

Fish & Shellfish Immunology ◽

10.1016/j.fsi.2014.05.020 ◽

2014 ◽

Vol 39 (2) ◽

pp. 280-284 ◽

Cited By ~ 36

Author(s):

Geovana Dotta ◽

Jaqueline Inês Alves de Andrade ◽

Eduardo Luiz Tavares Gonçalves ◽

Aline Brum ◽

Jacó Joaquim Mattos ◽

...

Keyword(s):

Nile Tilapia ◽

Lysozyme Activity ◽

Aloe Barbadensis ◽

Natural Extracts

Download Full-text

The cytokine midkine supports neutrophil trafficking during acute inflammation by promoting adhesion via β2 integrins (CD11/CD18)

Blood ◽

10.1182/blood-2013-06-510875 ◽

2014 ◽

Vol 123 (12) ◽

pp. 1887-1896 ◽

Cited By ~ 34

Author(s):

Ludwig T. Weckbach ◽

Anita Gola ◽

Michael Winkelmann ◽

Sascha M. Jakob ◽

Leopold Groesser ◽

...

Keyword(s):

Inflammatory Response ◽

Acute Inflammation ◽

Acute Inflammatory Response ◽

Key Points ◽

Β2 Integrins

Key Points MK promotes PMN recruitment during the acute inflammatory response. MK and β2 integrins (CD11/CD18) cooperate in mediating PMN adhesion during acute inflammation.

Download Full-text

Depletion of Neutrophils Exacerbates the Early Inflammatory Immune Response in Lungs of Mice Infected withParacoccidioides brasiliensis

Mediators of Inflammation ◽

10.1155/2016/3183285 ◽

2016 ◽

Vol 2016 ◽

pp. 1-17 ◽

Cited By ~ 9

Author(s):

Paula Andrea Pino-Tamayo ◽

Juan David Puerta-Arias ◽

Damaris Lopera ◽

Martha Eugenia Urán-Jiménez ◽

Ángel González

Keyword(s):

Monoclonal Antibody ◽

Immune Response ◽

Inflammatory Response ◽

Paracoccidioides Brasiliensis ◽

Yeast Cells ◽

Histopathological Analysis ◽

Acute Inflammatory Response ◽

Fungal Load ◽

Inflammatory Immune Response

Neutrophils predominate during the acute phase of theParacoccidioides brasiliensisinfection. Herein, we determined the role of the neutrophil during the early stages of experimental pulmonary paracoccidioidomycosis using a monoclonal antibody (mAb) specific for neutrophils. Male BALB/c mice were inoculated intranasally with1.5×106or2×106P. brasiliensisyeast cells. The mAb was administered 24 h before infection, followed by doses every 48 h until mice were sacrificed. Survival time was evaluated and mice were sacrificed at 48 h and 96 h after inoculation to assess cellularity, fungal load, cytokine/chemokine levels, and histopathological analysis. Neutrophils from mAb-treated mice were efficiently depleted (99.04%). Eighty percent of the mice treated with the mAb and infected with1.5×106yeast cells died during the first two weeks after infection. When mice were treated and infected with2×106yeast cells, 100% of them succumbed by the first week after infection. During the acute inflammatory response significant increases in numbers of eosinophils, fungal load and levels of proinflammatory cytokines/chemokines were observed in the mAb-treated mice. We also confirmed that neutrophils are an important source of IFN-γand IL-17. These results indicate that neutrophils are essential for protection as well as being important for regulating the early inflammatory immune response in experimental pulmonary paracoccidioidomycosis.

Download Full-text

Rapid dendritic cell recruitment is a hallmark of the acute inflammatory response at mucosal surfaces.

Journal of Experimental Medicine ◽

10.1084/jem.179.4.1331 ◽

1994 ◽

Vol 179 (4) ◽

pp. 1331-1336 ◽

Cited By ~ 284

Author(s):

A S McWilliam ◽

D Nelson ◽

J A Thomas ◽

P G Holt

Keyword(s):

Dendritic Cell ◽

Inflammatory Response ◽

Cellular Response ◽

Acute Inflammation ◽

Protective Mechanism ◽

Acute Inflammatory Response ◽

Normal Epithelium ◽

General Applicability ◽

Regional Lymph Nodes ◽

Mucosal Surfaces

Immunohistochemical analysis of challenge sites such as skin and the peritoneal cavity has identified neutrophils as virtually the sole cellular participants in acute bacterial inflammation, peak influx occurring 24-48 h in advance of mononuclear cell populations associated with adaptive immunity. This study challenges the general applicability of this paradigm. We demonstrate here that the earliest detectable cellular response after inhalation of Moraxella catarrhalis organisms is the recruitment of putative class II major histocompatibility complex-bearing dendritic cell (DC) precursors into the airway epithelium, the initial wave arriving in advance of the neutrophil influx. Unlike the neutrophils which rapidly transit into the airway lumen, the DC precursors remain within the epithelium during the acute inflammatory response where they differentiate, and develop the dendriform morphology typical of resident DC found in the normal epithelium. During the ensuing 48-h period, these cells then migrate to the regional lymph nodes. No comparable DC response was observed after epidermal or intraperitoneal challenge, and it may be that mucosal surfaces are unique in their requirement for rapid DC responses during acute inflammation. We hypothesize that the role of the DC influx during acute inflammation may be surveillance for opportunistic viruses, and that this covert protective mechanism is operative at a restricted number of mucosal tissue sites.

Download Full-text

The way of self-defence of the organism: inflammation

Orvosi Hetilap ◽

10.1556/oh.2013.29670 ◽

2013 ◽

Vol 154 (32) ◽

pp. 1247-1255 ◽

Cited By ~ 1

Author(s):

Lajos Jakab

Keyword(s):

Immune Response ◽

Inflammatory Response ◽

Innate Immune Response ◽

Acute Phase ◽

Acute Inflammation ◽

Serum Proteins ◽

Acute Phase Reactant ◽

Innate Immune ◽

Sterile Inflammation ◽

Phase Reactant

The acute and chronic constitutional reactions of the organism elicited by sterile causes and pathogenic structures threatening the soundness of the organism are surveyed by the author. It is emphasized that depending on causes which can be very different, there are various syndromes occurring in the clinical practice. On the basis of multitudiness of pathogenic factors and individual differences, the infammatory reactions are clinically, pathologically and pathobiochemically can be hugely variable. The acute inflammatory response may be sterile. It is often difficult to recognize in these processes whether the inflammation is harmful or beneficial for the organism as a whole. It is possible that the inflammatory response itself is the defending resource of the individual. The non-sterile acute inflammation is evoked by pathogenic microorganisms. The variety of clinical syndromes are explained by the high diversity of pathogenic microbes, the individualities of the defending organisms, and the natural and adaptive immunity of the organism which may be intact or possibly defective. In the latter case the inflammation itself is the disease, as a consequence of a pathological process conducted by the cortico-hypothalamo-adernal axis. The acute inflammation is a defending, preventing and repairing process, constituting an important part of the natural innate immune response. It is inseparable from the natural innate immune response, which is in close cooperation with the adaptive, specific immune response with mutual effects on each of the other. The conductor and the response reactions of the two immune responses are also the same. There are alterations in serum proteins/glycoproteins synthesized mostly by the hepatocytes. Because the concentration of almost all proteins/glycoproteins may change, the use of the discriminative term “acute phase reactant” is hardly relevant. For example, the HDL molecule is a negative “acute phase reactant”. On the gound of clinical, pathological and biochemical caracteristics, the chronic sterile inflammation is a very different entity. It has been established that atherosclerosis is one of the ab origine chronic inflammatory syndrome. It is a long-lasting pathological entity progressing, rather than resolving with different celerity, namely a unique vasculitis syndrome. We are speaking about risk factors instead of causes, which constitute larger or smaller groups to elicite the preventing reaction of the host. The propagations and final outcomes are quite different from that of the acute process. The disadvantages or benefits for the organism are scarcely predictable, albeit the chronic process may have roles in its prolonged nature. Orv. Hetil., 2013, 154, 1247–1255.

Download Full-text

Analysis of the immune response in padel

Fizicko vaspitanje i sport kroz vekove ◽

10.5937/spes2102134c ◽

2021 ◽

Vol 8 (2) ◽

pp. 134-140

Author(s):

Cádiz Pía ◽

Carlos Otín ◽

Luis Páez ◽

la de

Keyword(s):

Immune Response ◽

Inflammatory Response ◽

Physiological Response ◽

Acute Inflammatory Response ◽

General State ◽

Sports Performance ◽

Before And After ◽

Anti Inflammatory ◽

High Level ◽

Sports Activities

High intensity exercise and sports activities are closely related to a general state of inflammation that can lead to immunosuppression. This physiological response could decrease sports performance and even compromise the athlete's health. The objective of this study was to investigate the acute inflammatory response of a padel match. 15 elite players (28.2±7.9 years) participated voluntarily in the study. Different pro-inflammatory (IL-1ß, IL-2, IL-6, IL-7, IL-8, IL-12 and TNFa) and anti-inflammatory cytokines (IL-5, IL-10 and IL-13) were analyzed before and after a match. The results showed a decrease in IL-7 (p=0.007) and IL-8 (p<0.03) and increases in IL-10 (p<0.04). The results obtained suggest that the practice of high-level padel induces an anti-inflammatory response.

Download Full-text

Resolution in an ‘over-inflamed’ era

The Biochemist ◽

10.1042/bio03904004 ◽

2017 ◽

Vol 39 (4) ◽

pp. 4-7

Author(s):

Mauro Perretti ◽

Trinidad MonteroMelendez

Keyword(s):

Inflammatory Response ◽

Acute Inflammation ◽

Pharmacological Intervention ◽

Acute Inflammatory Response ◽

Moderate Pain ◽

Antiinflammatory Drugs ◽

Protective Mechanisms ◽

Life Saving ◽

Common Perception

Unlike other pathologies, inflammation is a condition that all individuals experience in their lives. Toothache, sunburn, a twisted ankle or cutting your hand while slicing bread, they all evoke what we call an acute inflammatory response. This type of response normally displays the cardinal signs of inflammation originally described by Aulus Cornelius Celsus: redness, swelling, heat and pain. Acute inflammation does not normally require any therapeutic intervention other than perhaps a painkiller, as it resolves, with the damage being naturally repaired. Inflammation is also at the root of many other diseases in a more ‘silent’ way as the cardinal signs of inflammation are not so evident. It is now appreciated that inflammatory mechanisms and processes contribute to the pathogenesis of a number of conditions including obesity, cancer, rheumatoid arthritis, atherosclerosis and diabetes. These are examples of chronic inflammation, arising either by the persistence of the injurious element causing it, or by a defect in our endogenous natural protective mechanisms grouped under the terminology of pro-resolving mechanisms. A common perception, likely to have been enhanced by the large variety of nonprescription antiinflammatory drugs available to anyone experiencing mild-to-moderate pain, is that inflammation is something harmful that must be stopped. In the next sections we will discuss on the protective life-saving role of the inflammatory response, the existence of our own body's resolutive mechanisms that regulate it and on when and why we need a pharmacological intervention to treat inflammation.

Download Full-text

PathoBiochemistry-directed Guidelines for COVID-19

10.20944/preprints202106.0653.v1 ◽

2021 ◽

Author(s):

Yuliya Buinitskaya ◽

Clifford Wlodaver ◽

Roman Gurinovich ◽

Siarhei Kastsiuchenka

Keyword(s):

Nitric Oxide ◽

Immune Response ◽

Severe Acute Respiratory Syndrome ◽

Inflammatory Response ◽

Therapeutic Interventions ◽

Health Conditions ◽

No Production ◽

Acute Inflammatory Response ◽

Therapeutic Guidelines ◽

Oxidant Production

Patients with underlying health conditions are at risk for a poor outcome from Coronavirus disease 2019 (COVID-19). Using machine reasoning by the sci.AI system, we investigated the pathobiochemistry of this observation to generate therapeutic guidelines. Facts were extracted and linked from publications available in nlm.nih.gov and Europe PMC to form the dataset which was validated by medical experts. Previously we described how preexisting chronic inflammation renders the acute inflammatory response to Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) excessive translating the SARS-CoV-2 infection into the clinical COVID-19 syndrome. Herein we focus on therapeutic interventions that mitigate the immune response. In essence, from bench to bedside, as depicted in the Graphical Abstract, the clinical management of COVID-19 should aim at: A. Control of excessive oxidant production. B. Neutralization of excessive oxidants. C. Upregulation of nitric oxide (NO) production.

Download Full-text

Hematopoietic progenitor kinase 1 (HPK1) is required for LFA-1–mediated neutrophil recruitment during the acute inflammatory response

Blood ◽

10.1182/blood-2012-08-451385 ◽

2013 ◽

Vol 121 (20) ◽

pp. 4184-4194 ◽

Cited By ~ 18

Author(s):

Sascha M. Jakob ◽

Robert Pick ◽

Doris Brechtefeld ◽

Claudia Nussbaum ◽

Friedemann Kiefer ◽

...

Keyword(s):

Inflammatory Response ◽

Acute Inflammation ◽

Neutrophil Recruitment ◽

Acute Inflammatory Response ◽

Hematopoietic Progenitor ◽

Key Points

Key Points Hematopoietic progenitor kinase 1 (HPK1) regulates LFA-1 affinity and thereby controls adhesion and postadhesion functions of neutrophils. Hematopoietic progenitor kinase 1 (HPK1) is critically involved in neutrophil trafficking during acute inflammation.

Download Full-text