scholarly journals Antioxidant activity and phenolic content of leaf infusions of Myrtaceae species from Cerrado (Brazilian Savanna)

2015 ◽  
Vol 75 (4) ◽  
pp. 948-952 ◽  
Author(s):  
L. K. Takao ◽  
M. Imatomi ◽  
S. C. J. Gualtieri
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Activity Index ◽  
Antioxidant Properties ◽  
Natural Antioxidants ◽  
Brazilian Savanna ◽  
Total Phenolic ◽  
Plant Materials ◽  
Dpph Method

Abstract There is considerable interest in identifying new antioxidants from plant materials. Several studies have emphasized the antioxidant activity of species belonging to the Myrtaceae family. However, there are few reports on these species from the Cerrado (Brazilian savanna). In this study, the antioxidant activity and phenolic content of 12 native Myrtaceae species from the Cerrado were evaluated (Blepharocalyx salicifolius, Eugenia bimarginata, Eugenia dysenterica, Eugenia klotzschiana, Hexachlamys edulis, Myrcia bella, Myrcia lingua, Myrcia splendens, Myrcia tomentosa, Psidium australe, Psidium cinereum, and Psidium laruotteanum). Antioxidant potential was assessed using the antioxidant activity index (AAI) by the DPPH method and total phenolic content (TPC) by the Folin–Ciocalteu assay. There was a high correlation between TPC and AAI values. Psidium laruotteanum showed the highest TPC (576.56 mg GAE/g extract) and was the most potent antioxidant (AAI = 7.97, IC50 = 3.86 µg·mL−1), with activity close to that of pure quercetin (IC50 = 2.99 µg·mL−1). The extracts of nine species showed IC50 of 6.24–8.75 µg·mL−1. Most species showed TPC and AAI values similar to or higher than those for Camellia sinensis, a commonly consumed tea with strong antioxidant properties. The results reveal that the analyzed Myrtaceae species from the Cerrado possess high phenolic contents and antioxidant activities. Thus, they are a potential source of new natural antioxidants.

Phytochemical and antioxidant properties of Algerian Medicago Sativa L. Water, Ethenol and Chloroform extract

2019 ◽  
Vol 9 (3) ◽  
pp. 80-86
Author(s):  
Hanane Megdad ◽  
Samuel Chiomokhare ◽  
Ahmed M’Hamedi ◽  
Paul L Chazot
Keyword(s):  
Antioxidant Activity ◽  
Medicago Sativa ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Medicago Sativa L ◽  
Ethanol Extracts

Plant natural products have a long history of being a rich source of neuroactive drugs.The total phenolic and flavonoid content, and antioxidant activity of ethanol, chloroform and aqueous extracts of AlgerianMedicagoSativaL, leaves were measured to discover new potential sources of Algerian natural antioxidants and neuroactives. All extracts were non-neurotoxic up to 1 mg/ ml for 24 h exposure. The total phenolcontent of M.Sativa extracts ranged between 38 and 69 mg GAE per g extracts. All of the extracts displayed antioxidant capacityassessed by two methods, but at different levels depending on the concentration and the extraction solvent used. The overall antioxidant potency was in the order water > chloroform> ethanol extracts, and the phenolic content exhibited a positive correlation with the antioxidant activity of M. Sativa extracts.Despite a weaker antioxidant activity, the ethanol extract elicited a calcium spike and suppressed the KCl-induced depolarisation response in neurons, indicating effect on ligand-and/or voltage gated calcium channels. In contrast, the chloroform and water extractsdisplayed no apparent direct functional effects. In conclusion, the antioxidant and functional properties related to phenolic content of Algerian Medicago Sativa L. in water and ethanol extracts, respectively.

scholarly journals Antioxidant Activities and Total Phenolics of Acacia Honey

2009 ◽  
Vol 27 (Special Issue 1) ◽  
pp. S245-S247 ◽  
Author(s):  
M. Krpan ◽  
K. Marković ◽  
G. Šarić ◽  
B. Skoko ◽  
M. Hruškar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Antiradical Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Optical Rotation ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Acacia Honey

The antioxidant activities and total phenolic content of 30 samples of acacia honey from Croatian territory were analysed. Phenolics were determined by the modified Folin-Ciocalteu method, antiradical activity by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and potential antioxidant activity using the ferric reducing antioxidant power (FRAP) method. In all samples, physicochemical parameters (water content, electrical conductivity, total reducing sugars, sucrose content, acidity, hydroxymethylfurfural content, prolin content, optical rotation, diastase activity and invertase activity) were measured according to Croatian legislation and International regulatory standards. Honey can be considered as a dietary supplement as it contains some important components including α-tocopherol, ascorbic acid, flavonoids and phenolics. The composition and properties of honey are dependent on floral origins, climatic conditions of the produced area, processing and storage methods. The results of physicochemical analyses showed that all the values of investigated parameters are in agreement with the current legislation. Phenolic content ranged from 31.72 mg/kg to 80.11 mg/kg, antiradical activity expressed as IC50 ranged from 61.28% to 253.47% and antioxidant activity expressed as FRAP value from 6.95 to 142.43. A positive correlation was observed between total phenolic content and antioxidant activity, indicating that phenolic compounds are mainly responsible for the antioxidant power of acacia honey.

scholarly journals Bekul Fruit, Potential Pharmafood from Northern Region of Bali Island, Indonesia: Selected Phytochemical Analyses and Antioxidant Activity of Its Ethanol Extract.

2021 ◽  
Vol 14 (1) ◽  
pp. 477-483
Author(s):  
Agung Nova Mahendra ◽  
I Nyoman Tri Pramartha
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Activity Index ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Northern Region ◽  
Total Phenolic ◽  
Tannin Content ◽  
Dpph Assay ◽  
Gallic Acid Equivalent

Background: Bekulfruit from Northern region (Buleleng regency), Bali, Indonesia, is commonly consumed fresh in the island of Bali or processed as local delicacy and used as part of religious offerings. Up to date, there is no data regarding the taxonomy, phytochemical composition, and antioxidant properties of this Balinese fruit.This study was aimed to investigate total phenolic content, tannin content and antioxidant activity of the ethanol extract of bekul fruit obtained from Banjar district, Buleleng regency, Bali. Methods: Total phenolic compound was quantified in terms of gallic acid equivalent (GAE) by using Folin-Ciocalteu method, mean while tannin content was determined in terms of tannic acid equivalent (TAE). IC50of the extract was determined using DPPH assay, and subsequently used in the calculation of antioxidant activity index (AAI) using the formula of Scherer and Godoy (2009). Results: Bekul plant was revealed as Ziziphusjujuba Mill. Total phenolic and tannin content of the extract was 29.48 mg/100 g GAE and 91.06 mg/100 g TAE, respectively. Thevalue of IC50was 77.40 mg/ml, with antioxidant activity index (AAI) of 50.94. Conclusion:Ethanol extract of bekul (Ziziphusjujuba Mill.) fruit contains phenolic and tannin compounds. This extract is found to scavenge free radicals and possess very strong antioxidant activityin vitro. Taken together, these findings lead to the notion that bekul fruit from Northern region of Bali, Indonesia, is a promising pharma food

Phytochemical Screening, Antioxidant Activity and Total Phenolic Content of Spirogyra spp.

2013 ◽  
Vol 699 ◽  
pp. 693-697 ◽  
Author(s):  
Rattanabhorn Junthip ◽  
Doungporn Amornlerdpison ◽  
Thitiphan Chimsook
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Rapid Test ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Abts Assay

A freshwater macroalga, Spirogyra spp., were analyzed for its phytochemical composition, antioxidant activity and total phenolic content (Folin−Ciocalteu method). Phytochemical analysis of Spirogyra neglecta revealed presence of phenolics, tannins, glycosides and saponins. The crude extraction of Spirogyra spp. was carried out using two solvents via, methanol (methanolic extract) and water (aqueous extract). The total phenolic contents of crude extracts were shown at 346.58±1.61 and 589.77±1.65 mg gallic acid equivalents/g extract in aqueous and methanolic extracts, respectively. The antioxidant capacity of aqueous and methanolic extract was estimated by two different methods; ABTS assay, and DPPH assay. The antioxidant activity of two extracts is affected by the extracting solvent and different assay. In the DPPH scavenging assay and ABTS assay, both extracts showed high antioxidant activity. In addition, the high correlation between results of all antioxidant activities and total phenolic content was found. The rapid TLC assay in methanolic extract is considered as the rapid test to evaluate the antioxidant activity of natural compounds. The compounds showing four bands at Rf = 0.25, 0.35, 0.42, 0.64. This study showed that Spirogyra spp. might constitute an important source of natural antioxidants.

scholarly journals Olive Paste-Enriched Cookies Exert Increased Antioxidant Activities

2021 ◽  
Vol 11 (12) ◽  
pp. 5515
Author(s):  
Eleni-Anna Argyri ◽  
Stylianos-Panagiotis Piromalis ◽  
Antonios Koutelidakis ◽  
Dimitrios Kafetzopoulos ◽  
Andreas S. Petsas ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Total Phenolics ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Strongly Correlated ◽  
Unsaturated Fat ◽  
Daily Diet ◽  
The Impact

Functional foods are beneficial to human health and are part of the daily diet of people trying to follow a healthier lifestyle. Olive paste is a good source of functional compounds, mainly phenolic compounds, that have been shown to have health benefits. At the same time, cookies are an ideal snack that can be fortified with additional ingredients to address human dietary needs. The study aimed to enrich cookies with olive paste and extra ingredients for flavor differentiation and evaluate the impact of the enrichment on their antioxidant properties. Enriched cookies were prepared analyzed and tested for sensorial acceptability, total phenolics, and antioxidant activities by DPPH, ABTS, FRAP, and CUPRAC assays. Enriched cookies were sensorially acceptable. Unsaturated fat, total phenolics, and antioxidant activities of enriched cookies were higher compared to control cookies, while among enriched cookies the extra addition of 1% garlic, 0.5% thyme, and 0.5% oregano resulted in higher total phenolics and antioxidant activities compared to cookies that were flavored either with 3% vegetables or 3% orange zest. Antioxidant activity in cookies was strongly correlated with total phenolic content. Cookies enriched with olive paste may be healthy functional food in terms of increased antioxidant activity.

scholarly journals Phenolic content and antioxidant activity of Echinocystis lobata (Mich.) Torr. ET Gray (Cucurbitaceae)

10.5219/1579 ◽  
2021 ◽  
Vol 15 ◽  
pp. 784-791
Author(s):  
Yulia Vinogradova ◽  
Olga Shelepova ◽  
Olena Vergun ◽  
Olga Grygorieva ◽  
Jan Brindza
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Echinocystis Lobata

Echinocystis lobata (Mich.) Torr. et Gray extracts were investigated for their bioactive compounds and their potential for natural antioxidant properties. In vitro antioxidant activity of methanol, ethanol, and water extracts were evaluated by 1,1-diphenyl-picrylhydrazyl (DPPH) radical scavenging activity. Total phenolic content (TPC) and total flavonoid content (TFC) of fruits and seeds were determined. TPC is a fairly constant value and varies weakly both among the different organs of Echinocystis lobata and among the geographical locations of the populations studied. Like other invasive species, Echinocystis lobata is superior to many other plants in terms of the total amount of flavonoids contained in its fruits and seeds. The dried fruits contain more flavonoids than the seeds. Seeds from Slovak populations have higher flavonoid content than those from Russian populations. The results demonstrated that Echinocystis lobata can be a good source of natural antioxidants and have a great potential for commercialization, especially in the pharmaceutical industry.

scholarly journals Correlation of Total Phenolic, Flavonoid and Carotenoid Content of Sesbania sesban (L. Merr) Leaves Extract with DPPH Scavenging Activties

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Sani N. Fitriansyah ◽  
Irda Fidrianny ◽  
Komar Ruslan
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Sesbania Sesban ◽  
Total Carotenoid Content ◽  
Dpph Method ◽  
Dpph Scavenging

Antioxidants are found in many plants and can neutralize free radicals. Sesbania sesban (L.)Merr is a plant that has been used empirically by Indonesian people and its proven have many pharmacologycal activity. The objectives of this research were to study antioxidant activity Sesbania sesban leaves extract using DPPH (2,2-diphenyl-1-picrylhydrazyl) method and correlation with its total phenolic, flavonoid and carotenoid content. Extraction was performed by reflux using different polarity solvent. The extracts were vaporated using rotary evaporator. Antioxidant activity was tested using DPPH assay. Determination of total phenolic, flavonoid and carotenoid content were performed by UV-visible spectrophotometry and its correlation with inhibitory concentration 50 (IC50) DPPH scavenging activity were analyzed by Pearson’s method. Nhexane, ethyl acetate and ethanol extracts of Sesbania sesban leaves had IC50 DPPH  50 g/ml and it can be classified as very strong antioxidant. Ethanol extract of Sesbania sesban had the highest of total phenolic content (5.18 g GAE/100 g) and highest total flavonoid content (4.56 g QE/100 g), while the highest total carotenoid content (4.56 g BE/100 g) was given by n-hexane extract. Total phenolic content in Sesbania sesban leaves extracts had significant and negative correlation with their IC50 DPPH scavenging activities. Phenolic compounds in Sesbania sesban leaves extracts were contributor major in its antioxidant activities by DPPH method.

scholarly journals DPPH Scavenging Activities and Phytochemical Content of Four Asteraceae Plants

2017 ◽  
Vol 9 (6) ◽  
Author(s):  
Raden Bayu Indradi ◽  
Irda Fidrianny ◽  
Komar Ruslan Wirasutisna
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Dpph Method

Free radicals are atoms or molecules that have one or more unpaired electrons on its outer orbital, highly reactive, and could damage cell inside human body. Human body produce antioxidant to neutralize free radicals, but human ageing and stress oxidative conditions would increase the formation of free radicals, therefore an exogenous antioxidant are needed. Asteraceae family is the largest family among the plant kingdom therefore it has great potential as source of exogeneous antioxidants. The objectives of this research were to determine antioxidant activities of aerial part of elephant’s foot (Elephantopus scaber L.), false daisy (Eclipta alba (L.) Hassk.), Indian pluchea (Pluchea indica (L.) Less), and dandelion (Taraxacum officinale Weber ex F.H Wigg.) using DPPH method, determine total flavonoid and total phenolic content, and analyze correlation between total flavonoid content and total phenolic content with antioxidant activity. Extraction was carried out by reflux with increasing polarity using n-hexane, ethyl acetate, and ethanol respectively. Antioxidant activity was determined by DPPH method. Total flavonoid content was determined using Chang’s method and total phenolic content evaluated using Folin—Ciocalteu reagent. Correlation of total flavonoid content and total phenolic content was analyzed by Pearson’s method. Ethanolic extract of Indian pluchea showed the highest antioxidant activity with IC50 DPPH 16.66 ± 0.08 µg/mL. The highest total phenolic content (23.49 ± 0,56 g QE (Quercetin Equivalent)/100 g) was given by ethyl acetate extract of Indian pluchea, while the highest flavonoid content (16.48 ± 0.25 g GAE (Gallic Acid Equivalent)/100 g) was showed by ethanolic extract of Indian pluchea. Total phenolic content of elephant’s foot, false daisy and Indian pluchea herbs extracts showed significantly negative correlation with their IC50 of DPPH scavenging activities. Indian pluchea herbs extract had the highest antioxidant activity using DPPH method compared to elephant’s foot, false daisy and dandelion herbs. Phenolic compounds were the major contributor in antioxidant activities of elephant’s foot, false daisy and dandelion herbs extracts by DPPH method.

scholarly journals Determination of the Phenolic Content in Iranian Trehala Manna and Evaluation of Their Antioxidant Effects

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Farzaneh Darikvand ◽  
Mehrdad Ghavami ◽  
Masoud Honarvar
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Major Type ◽  
Total Phenolic ◽  
Ferric Ion ◽  
Pharmaceutical Industries

One of the most challenging issues in the food and pharmaceutical industries is finding effective and safe antioxidants from natural resources compared to their synthetic compounds, which have side effects. In this regard, Trehala manna was considered a great antioxidant source categorized as the major type of manna produced naturally and by the Echinops plant in response to insect activity. In this study, the antioxidant activity and phenolic content of the numerous Trehala manna in Echinops sp. have been investigated. Different methods of radical scavenging activity comprising 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) have been investigated to evaluate antioxidant activity. The phenolic contents were measured by Folin–Ciocalteu and standard phytochemical methods. Quantitative and qualitative amounts of phenolic content, including caffeic, ferulic, coumaric, syringic, and hydroxybenzoic acids, were quantified by high-performance liquid chromatography (HPLC). The results indicated the significant changes in the amounts of phenolics and the antioxidant properties in Trehala manna samples, based on the place of collection. Based on results, antioxidant capacity detected by DPPH and ABTS tests showed the IC50 values of 40–94 µg/mL and 28–72 µg/mL, respectively. Results of the FRAP test represented very strong ferric ion reducing activities (0.04–0.83 mmol Fe2+/g). Ferric ion reducing data were not markedly different from ABTS and DPPH ones. These samples also presented the highest phenolic content (1.32–2.28 mg GAE/100 g). Jahrom Trehala manna was the highest in both phenolic content and antioxidant value, while Sabzevar was the lowest. We found a significant relationship between the antioxidant values and total phenolic counts. It indicates that the phenolics contribute to the observed antioxidant activities of these samples.

scholarly journals Evaluation of antioxidant activity of Melittis melissophyllum L. extracts

2014 ◽  
Vol 66 (4) ◽  
pp. 1401-1410 ◽  
Author(s):  
Slavica Grujic ◽  
Gordana Stojanovic ◽  
Violeta Mitic ◽  
Vesna Stankov-Jovanovic ◽  
Ana Dzamic ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Hierarchical Cluster ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Scavenging Effect ◽  
Aerial Parts ◽  
Ic50 Values ◽  
Promising Source

The antioxidant activities of methanol and ethanol (10%, 30%, 50% and 96%) extracts of the aerial parts of Melittis melissophyllum L. were determined by DPP H, ABTS and FRAP assays. The content of flavonoids and phenols was also investigated. The total phenolic content in the extracts was determined using Folin-Ciocalteu assay; their amounts ranged between 63.5 and 111.7 mg GAeqv/g, while the concentrations of flavonoids were from 7.33 to 56.00 mg Queqv/g. IC50 values of the DPP H scavenging effect were from 0.109 to 0.664 mg/mL. The DPP H scavenging effect of the extracts was determined and the obtained IC50 values were from 0.109 to 0.664 mg/mL of solution. The values of ABTS radical activity were from 0.45 to 0.89 mg ascorbic acid/g. The FRAP value was within the range 0.160-0.382 mmol Fe/mg. The obtained values were analyzed by means of multivariate analysis, employing a hierarchical cluster analysis and between-groups linkage. The presented results confirmed that M. melissophyllum possesses good antioxidant properties and may serve as a promising source of natural antioxidants.

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