scholarly journals Spectral analysis and Antibacterial activity of the bioactive principles of Sargassum tenerrimum J. Agardh collected from the Red sea, Jazan, Kingdom of Saudi Arabia

2023 ◽  
Vol 83 ◽  
Author(s):  
M. Albratty ◽  
H. A. Alhazmi ◽  
A. M. Meraya ◽  
A. Najmi ◽  
M. S. Alam ◽  
...  
Keyword(s):  
Petroleum Ether Extract ◽  
Acid Methyl Ester ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Bioactive Components ◽  
Kingdom Of Saudi Arabia ◽  
Spectrometry Analysis ◽  
Benzenedicarboxylic Acid ◽  
Sargassum Tenerrimum ◽  
Pharmaceutical Molecules

Abstract Seaweeds are a major marine resource that can be explored to develop novel pharmaceutical molecules. The present study showed the presence of unique bioactive components in the petroleum ether extract (PEE) and methanolic extract (ME) of Sargassum tenerrimum. The gas chromatography-mass spectrometry analysis suggested that the PEE of S. tenerrimum contained antibacterial biomolecules: hexadecanoic acid, methyl ester, 17-pentatriacontene, dasycarpidan-1-methanol, and acetate (ester). However, the ME of S. tenerrimum exhibited better antibacterial effect than the PEE due to the presence of the bioactive compounds 1,2-benzenedicarboxylic acid, diisooctyl ester, tetratetracontane, 1-docosene, 1,2-benzenediol, and benzoic acid. Thus, promising antibacterial molecules can be isolated from S. tenerrimum for better therapeutic use.

scholarly journals GAS CHROMATOGRAPHY–MASS SPECTROMETRY ANALYSIS OF METHANOL EXTRACT OF ZANTHOXYLUM RHETSA DC. FRUITS

2018 ◽  
Vol 11 (12) ◽  
pp. 247
Author(s):  
Kuladip Gurav ◽  
Varsha Jadhav (rathod)
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Biological Activities ◽  
Acid Methyl Ester ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Hexadecanoic Acid ◽  
Spectrometry Analysis ◽  
Chromatography Mass Spectrometry

Objective: The aim was to investigate important bioactive compounds, biological activities, and medicinal importance of Zanthoxylum rhetsa fruits.Methods: The present work was carried out by gas chromatography–mass spectrometry (GC-MS) method for the identification of different compounds.Result: The methanolic extract of fruits showed 32 chemical compounds which are identified through GC-MS analysis. Among them, some of the compound names and percentage values are as follows: 2-propanone, 1,3-dihydroxy (48.9%), 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl (33.7%), 2-furancarboxaldehyde, 5-[hydroxymethyl] (50.2%), 1-Heptatriacontanol (34.4%), 9,12-octadecadienoic acid (zz)- (48.6%), cholestan- 3-ol,2-methylene, [3β,5α] (75.0%), 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl (90.8), 2-furancarboxaldehyde, 5-[hydroxymethyl] (92.0%), hydroquinone (64.9%), n-hexadecanoic acid (37.5%), octadecanoic acid (28.2%), 9,12,15, octadecatrienoic acid, 2-[(trimethylsiyl]oxy]-1- [(trimethylsiyl]oxy]methyl] ethyl ester, [zzz] (22.6%), 9-hexadecanoic acid (10.3%), digitoxin (18.8%), 8,11,14-eicosatrienoic acid methyl ester, [zzz] (25.5%), and oleic acid (16.5%). Most of the identified compounds in the crude methanolic extracts exhibit some bioactivities, namely anticancer, anti-inflammatory, antimicrobial, hepatoprotective, antioxidant, hypocholesterolemic, nematicide, pesticide, anti-androgenic flavor, hemolytic, 5-alpha-reductase inhibitor, insectifuge, antiarthritic, anti-coronary, cardiovascular, anti-breast cancer, aromatic, and insectifuge. On the basis of the above investigation, the fruits can be recommended as a treasure of bioactive compounds and it plays a promising role in herbal medicine.Conclusion: The present study reveals that fruits of Z. rhetsa contain various bioactive compounds. Digitoxin is recorded in the ripened fruit of Z. rhetsa and it shows the anticancerous and cardiac arrest properties. Hence, in future, this plant will play a promising role in curing cancer.

scholarly journals DETERMINATION OF BIOACTIVE COMPONENTS OF BARLERIA COURTALLICA NEES (ACANTHACEAE) BY GAS CHROMATOGRAPHY–MASS SPECTROMETRY ANALYSIS

2017 ◽  
Vol 10 (6) ◽  
pp. 273
Author(s):  
Ponmathi Sujatha A ◽  
Michael Evanjaline R ◽  
Muthukumarasamy S ◽  
Mohan Vr
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Root Extract ◽  
Bioactive Components ◽  
Spectrometry Analysis ◽  
Chromatography Mass Spectrometry ◽  
Ethanol Extracts

Objective: The present investigation was carried out to determine the possible bioactive components of stem, root, and leaf of Barleria courtallica Nees using a gas chromatography–mass spectrometry (GC-MS).Methods: The phytocomponents of the ethanol extracts of stem, root, and leaf of B. courtallica were investigated using PerkinElmer GC-MS, while the mass spectra of the compounds found in the extracts were matched with the National Institute of Standards and Technology version II library. Results: 25, 23, and 28 compounds were identified in the ethanol extracts of stem, root, and leaf of B. courtallica, respectively. The prevailing compounds of stem were β-sitosterol (20.27%), stigmasterol (17.07%), eicosane, 7-hexyl- (6.64%), 3,7,11,15-tetramethyl-2-hexadecan-1-ol (5.97%), and tetracosane, 11-decyl- (5.91%). The major constituents recorded from root extract of B. courtallica were β-sitosterol (22.94%), stigmasterol (20.17%), urs-12-en-28-oic acid, 3-hydroxy-, methyl ester, (3β)- (18.42%), and eiosane, 7-hexyl- (7.06%). The prevailing compounds of leaf were 3,7,11,15- tetramethyl-2-hexadecan-1-ol (34.42%), phytol (14.18%), β-sitosterol (12.71%), squalene (11.25%), stigmasterol (8.15%), phytol acetate (6.53%). Conclusions: From the results, it is evident that B. courtallica contains various bioactive compounds and is recommended as a plant of phytopharmaceutical importance.

scholarly journals GAS CHROMATOGRAPHY 􀇧 MASS SPECTROMETRY ANALYSIS OF ESSENTIAL OIL COMPOSITION OF CLERODENDRUM SERRATUM L.: A TRADITIONAL PLANT OF INDIA

2017 ◽  
Vol 10 (7) ◽  
pp. 226 ◽  
Author(s):  
Raj Kumar Tiwari ◽  
Udayabanu M ◽  
Silpi Chanda
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Acid Methyl Ester ◽  
Essential Oil Composition ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Molecular Formula ◽  
Oil Composition ◽  
Spectrometry Analysis ◽  
Chromatography Mass Spectrometry

Objective: Clerodendrum serratum (L.) belonging to family Verbenaceae, commonly known as Bharangi, is a popular plant across various communities of India. Gas chromatography-mass spectrometry (GC-MS) is a unique technique to prospect various volatile principles and semivolatile compounds.Materials and Methods: The phytoconstituents present in the root of n-hexane extract were evaluated by GC-MS using Clarus 500 mass spectrometer.Results and Discussions: A total of 15 constituents were identified and characterized from the roots of C. serratum L. All the 15 compounds were tabulated with their retention time, percentage peak area, molecular formula, molecular weight, and Chemical Abstracts Service number. Out of all the compounds, 60% comprises the oxygenated compounds. Compound hexadecanoic acid, methyl ester, and benzene, 1,3-bis(1,1-dimethylethyl) showed highest percentage about 6.5 and 74.9, respectively.Conclusion: This study showed the presence of 15 compounds ranging from alkane, alkene as longest chain along with ester, aromatic and nonaromatic hydrocarbons.

scholarly journals Evaluation of the Phytochemical Constituents of Extracts of Kigelia africana Fruit and Sorghum bicolor, Stalk in Lagos Nigeria

2021 ◽  
pp. 49-58
Author(s):  
Nkem F. Obianagha ◽  
Chukwuma J. Okafor ◽  
Ufuoma Chukwuani ◽  
Omobolanle Abioye Ogundahunsi ◽  
Steve O. Ogbonnia ◽  
...  
Keyword(s):  
Sorghum Bicolor ◽  
Chemical Constituents ◽  
Herbal Medicines ◽  
Acid Methyl Ester ◽  
Biological Action ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Indigenous Plants ◽  
Spectrometry Analysis ◽  
Phytochemical Constituents

The use of herbal medicines and phytonutrients or nutraceuticals with numerous secondary metabolites continues to expand rapidly across the world, with many people now resorting to these products to treat various health challenges in different national healthcare settings. Therefore, the study is aimed at evaluating the phytochemical composition in the fruit extract of Kigelia africana and Sorghum bicolor stalks bought from the Mushin market in Lagos, Nigeria to ascertain its numerous pharmacological activities and identify the various chemical compounds responsible for these activities. Ethanol extracts of identified fresh fruits of K. africana  and S. bicolor stalks (L) were prepared for phytochemical screening using established methods for Alkaloids, Tannins, Phenols, Flavonoids, and Terpenoids testing as well as Gas Chromatography-Mass Spectrometry Analysis (GC-MS) for the analysis of the extracts. The chemical constituents present in both extracts were tannins, phenols, flavonoids, and saponins as well as alkaloids found only in K. africana. Also, the chromatogram of K. africana revealed the presence of forty-four (44) phytochemical constituents. At the same time, that of S. bicolor were twenty-nine (29) phytochemical constituents that could contribute to the medicinal quality of the plant with 9, 12-Octadeadienoic acid (Z, Z), and 13-Docosenoic acid, methyl ester, (Z) found as the major compounds respectively. The K africana and S. bicolor African indigenous plants in Lagos, Nigeria, possess different phytocomponents of scientific importance, biological action, and potential medicinal properties. There is a need for more standardization and purification of this herbal formulation for the treatment of diseases.

scholarly journals Chemical Composition and Biological Activities of Seeds from Four Vitis vinifera Tunisian Varieties

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Thouraya Majoul ◽  
Faten Bouabdallah ◽  
Majdi Hammami ◽  
Hajer Satouri ◽  
Olfa Tabbene ◽  
...  
Keyword(s):  
Biological Activities ◽  
Sinapic Acid ◽  
Acid Methyl Ester ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Total Phenolic ◽  
Good Growth ◽  
Spectrometry Analysis ◽  
Beneficial Effects

Thousands of polyphenolic compounds have been identified in various plants. Recently, a number of studies showed that beneficial effects of grapes are related to the presence of polyphenols, with multiple biological activities. The aim of this study was to compare the polyphenol profiles as well as biological activities of the seeds of four Tunisia cultivated grape cultivars, syrat, merlot, cabernet sauvignon and carignan. The total phenolic content of seed extracts varied between varieties and seemed to be correlated to the scavenging activity. Preliminary antibacterial results showed a good growth inhibitory activity of syrat cultivar against Staphylococcus epidermidis. To our knowledge, this is the first of such study being performed on this bacterium, known as a leading cause of Tunisian hospital-acquired infections. Gas Chromatography Mass Spectrometry analysis identified 20 polyphenol components, flavonoids being the most abundant in all extracts, followed by phenolic acids, resveratrol, tyrosol, and syringaldehyde. We also noticed a deficiency of three phenolic acid compounds (salicylic acid methyl ester, ferulic acid, Sinapic acid) as well as a flavonoid one (myricetin) in merlot cultivar, which could be responsible for its low antioxidant activity. These results will help in the selective exploitation of the seeds obtained from winemaking wastes, as well as in further pharmacological and/or in vivo investigations.

Spectrophotometry Measurement of Polynuclear Aromatic Hydrocarbons in Hamilton Harbour Sediments

1991 ◽  
Vol 26 (1) ◽  
pp. 1-16 ◽  
Author(s):  
T.P. Murphy ◽  
H. Brouwer ◽  
M.E. Fox ◽  
E. Nagy
Keyword(s):  
Aromatic Hydrocarbons ◽  
Total Concentration ◽  
Coal Tar ◽  
Sediment Cores ◽  
Polynuclear Aromatic Hydrocarbons ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Near Surface ◽  
Spectrometry Analysis ◽  
Hamilton Harbour

Abstract Eighty-one sediment cores were collected to determine the extent of coal tar contamination in a toxic area of Hamilton Harbour. Over 800 samples were analyzed by a UV spectrophotometric technique that was standardized with gas chromatography/mass spectrometry analysis. The coal tar distribution was variable. The highest concentrations were near the Stelco outfalls and the Hamilton-Wentworth combined sewer outfalls. The total concentration of the 16 polynuclear aromatic hydrocarbons (PAHs) in 48,300 m3 of near-surface sediments exceeded 200 µg/g.

scholarly journals Biodegradation and metabolic pathway of fenvalerate by Citrobacter freundii CD-9

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jie Tang ◽  
Dan Lei ◽  
Min Wu ◽  
Qiong Hu ◽  
Qing Zhang
Keyword(s):  
Environmental Pollution ◽  
Environmental Remediation ◽  
High Efficiency ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Citrobacter Freundii ◽  
16S Rrna Sequence ◽  
Intracellular Enzyme ◽  
Pyrethroid Insecticides ◽  
Spectrometry Analysis

Abstract Fenvalerate is a pyrethroid insecticide with rapid action, strong targeting, broad spectrum, and high efficiency. However, continued use of fenvalerate has resulted in its widespread presence as a pollutant in surface streams and soils, causing serious environmental pollution. Pesticide residues in the soil are closely related to food safety, yet little is known regarding the kinetics and metabolic behaviors of fenvalerate. In this study, a fenvalerate-degrading microbial strain, CD-9, isolated from factory sludge, was identified as Citrobacter freundii based on morphological, physio-biochemical, and 16S rRNA sequence analysis. Response surface methodology analysis showed that the optimum conditions for fenvalerate degradation by CD-9 were pH 6.3, substrate concentration 77 mg/L, and inoculum amount 6% (v/v). Under these conditions, approximately 88% of fenvalerate present was degraded within 72 h of culture. Based on high-performance liquid chromatography and gas chromatography-mass spectrometry analysis, ten metabolites were confirmed after the degradation of fenvalerate by strain CD-9. Among them, o-phthalaldehyde is a new metabolite for fenvalerate degradation. Based on the identified metabolites, a possible degradation pathway of fenvalerate by C. freundii CD-9 was proposed. Furthermore, the enzyme localization method was used to study CD-9 bacteria and determine that its degrading enzyme is an intracellular enzyme. The degradation rate of fenvalerate by a crude enzyme solution for over 30 min was 73.87%. These results showed that strain CD-9 may be a suitable organism to eliminate environmental pollution by pyrethroid insecticides and provide a future reference for the preparation of microbial degradation agents and environmental remediation.

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