scholarly journals A comparative study on the virulence of entomopathogenic fungi against Trogoderma granarium (Everts) (Coleoptera: Dermestidae) in stored grains rice

2022 ◽  
Vol 82 ◽  
Author(s):  
J. Iqbal ◽  
S. Ahmad ◽  
Q. Ali
Keyword(s):  
Entomopathogenic Fungi ◽  
Insect Pests ◽  
Control Measure ◽  
Microbial Control ◽  
Immersion Method ◽  
Trogoderma Granarium ◽  
Khapra Beetle ◽  
Percent Mortality ◽  
Conidial Concentration

Abstract Entomopathogenic fungi (EPF) now a possible safer microbial control measure that could be considered as a substitute for chemical control of insect pests. Three EPF viz., Metarihizium anisopliae, Isaria furnosoroseus and Beauveria bassiana were evaluated for their virulence against the grubs of Khapra beetle, Trogoderma granarium (Everts) under laboratory conditions. The isolates were applied by two methods viz., diet incorporation and an immersion method with 3rd instar 20 grubs of T. granarium for each. The virulence of EPF was determined using percent mortality. Significantly higher mortality was observed in M. anisopliae applied through immersion (98.33%) and diet incorporation (93.33%) methods followed by B. bassiana (90.83 and 85.83%, respectively). The mortality caused by I. furnosoroseus was statistically lower in immersion and diet incorporation methods i.e. 81.67 and 73.33%, respectively. Based on the immersion method, all EPF were studied for multiple conidial concentration i.e., 1×104, 1×105, 1×106, 1×107 and 1×108 under the same in-vitro conditions. All the isolates were pathogenic to grub of T. granarium at the highest conidial concentration. M. anisopliae was proved the most effective virulent resulting in 98.33% mortality of the pest with LT50 4.61 days at 1 × 108 conidial concentration followed by 90.83 and 81.67 percent mortality with 5.07 and 8.01 days LT50, in the application of B. bassiana and I. furnosoroseus, respectively. M. anisopliae showed higher efficacy and could be considered as promising EPF for the development of myco-insecticides against effective biocontrol of T. granarium.

scholarly journals Biosynthesis of Silver Nanoparticles Mediated by Entomopathogenic Fungi: Antimicrobial Resistance, Nanopesticides, and Toxicity

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 852
Author(s):  
Tárcio S. Santos ◽  
Tarcisio M. Silva ◽  
Juliana C. Cardoso ◽  
Ricardo L. C. de Albuquerque-Júnior ◽  
Aleksandra Zielinska ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Filamentous Fungi ◽  
Entomopathogenic Fungi ◽  
Antimicrobial Agents ◽  
Insect Pests ◽  
Insect Control ◽  
Biological Synthesis ◽  
Biological Methods ◽  
Potential Use

Silver nanoparticles are widely used in the biomedical and agri-food fields due to their versatility. The use of biological methods for the synthesis of silver nanoparticles has increased considerably due to their feasibility and high biocompatibility. In general, microorganisms have been widely explored for the production of silver nanoparticles for several applications. The objective of this work was to evaluate the use of entomopathogenic fungi for the biological synthesis of silver nanoparticles, in comparison to the use of other filamentous fungi, and the possibility of using these nanoparticles as antimicrobial agents and for the control of insect pests. In addition, the in vitro methods commonly used to assess the toxicity of these materials are discussed. Several species of filamentous fungi are known to have the ability to form silver nanoparticles, but few studies have been conducted on the potential of entomopathogenic fungi to produce these materials. The investigation of the toxicity of silver nanoparticles is usually carried out in vitro through cytotoxicity/genotoxicity analyses, using well-established methodologies, such as MTT and comet assays, respectively. The use of silver nanoparticles obtained through entomopathogenic fungi against insects is mainly focused on mosquitoes that transmit diseases to humans, with satisfactory results regarding mortality estimates. Entomopathogenic fungi can be employed in the synthesis of silver nanoparticles for potential use in insect control, but there is a need to expand studies on toxicity so to enable their use also in insect control in agriculture.

scholarly journals USE OF INDIGENOUS ISOLATES OF METARHIZIUM, ISARIA AND BEAUVERIA AS POTENTIAL BIO-CONTROL AGENTS AGAINST Sitophilus oryzae UNDER LABORATORY CONDITIONS

2019 ◽  
Vol 56 (04) ◽  
pp. 1037-1044
Author(s):  
Saif-Ur Rehman
Keyword(s):  
Chemical Control ◽  
Multiple Dose ◽  
Insect Pests ◽  
Sitophilus Oryzae ◽  
Immersion Method ◽  
New Class ◽  
Laboratory Conditions ◽  
Dose Comparison ◽  
Fungal Isolates ◽  
Conidial Concentration

Entomopathogenic fungi (EPF) are suggested as a new class of alternates followed by Synthetic chemical control of the insect pests .Virulence of seven EPF isolates, four strains of Metarhizium {(Qin-08, Qin-13, Qin-18 and ME-38 (LT-178)}, two of Isaria {ME-33 (ILT-01), Yulin-5 (IYL-01)} and one of Beauveria bassiana (Qin-21) were assessed against the adults of Sitophilus oryzae under laboratory conditions, firstly at single conidial concentration (1x108 ml-1 ) by immersion and the food mix methods. Qin-21, ME-33 and Qin-18 caused significantly highest mortality of S.oryzae at immersion methods (100, 100 and 98%), followed by food mix method to 100, 84.16 and 91.66% respectively. Yulin-5 was the least effective, showing significantly the lowest mortality at food mixed (32.49%) and immersion methods (40.20%) respectively. Hence, the immersion method was found to be most effective, resulting the higher mortality rate of S. oryzae in comparison to food mix method, in all tested fungal isolates. Secondly, we screened out the most effective isolates for multiple dose comparison i.e., 1x104 to 1x107 conidia ml-1 by immersion method only. The isolate ME-33 resulted in 100% mortality of the pest at higher conidial dose as compared to Qin-21 and Qin-18 which showing 80 and 64.64% mortality respectively. The LT50 was observed to be 3.63, 4.17 and 8.58 days in ME-33, Qin-21 and Qin-18, respectively at the highest conidial concentration (1x107ml-1 ). ME-33 isolate with the highest mortality and lowest LT50 at conidial concentration 1x107 ml-1 proved to be most effective for the control of S. oryzae. So these fungal islotes could be a better alternative for the management of S. oryzae

scholarly journals Entomopathogenic Fungi: Factors Involved in Successful Microbial Control of Insect Pests

2020 ◽  
Vol 17 (2) ◽  
pp. 74-83
Author(s):  
Ghulam Ali Bugti ◽  
Wang Bin ◽  
Shafique Ahmed Memo ◽  
Ghulam Khaliq ◽  
Muhammad Abuzar Jaf
Keyword(s):  
Entomopathogenic Fungi ◽  
Insect Pests ◽  
Microbial Control

scholarly journals Dual effects of entomopathogenic fungi on control of the pest Lobesia botrana and the pathogenic fungus Eutypella microtheca on grapevine

2021 ◽  
Vol 54 (1) ◽  
Author(s):  
Juan Aguilera-Sammaritano ◽  
Juan Caballero ◽  
María Deymié ◽  
Melisa Rosa ◽  
Fabio Vazquez ◽  
...  
Keyword(s):  
Entomopathogenic Fungi ◽  
Developmental Stages ◽  
Insect Pests ◽  
Pathogenic Fungus ◽  
Insect Pest ◽  
Similar Efficacy ◽  
Lobesia Botrana ◽  
Phytopathogenic Fungus ◽  
Highly Effective

Abstract Background Entomopathogenic fungi (EPF) are the natural enemies of insect pests. Nevertheless, research on the use of EPF for simultaneous prevention of pest and disease agents on the same crop is limited. In this study, we explored the potential dual effects of three strains of the EPF Metarhizium anisopliae on the control of detrimental agents of Vitis vinifera L., including different developmental stages (larvae, pupae, and adult) of the insect pest Lobesia botrana and the phytopathogenic fungus Eutypella microtheca. Methods Laboratory pathogenicity trials were performed to examine the effects of the three M. anisopliae strains on the mortality rate of L. botrana. In addition, field trials were conducted to assess the biocontrol potential of one selected M. anisopliae strain on the larval stage of L. botrana. Moreover, inhibitory effects of the three EPF strains on E. microtheca growth were examined in vitro. Results All the M. anisopliae strains were highly effective, killing all stages of L. botrana as well as inhibiting the growth of E. microtheca. The in vitro mortality of larvae treated with the strains was over 75%, whereas that of treated pupae and adults was over 85%. The three EPF strains showed similar efficacy against larvae and adult stages; nevertheless, pupal mortality was observed to be strain dependent. Mortality of L. botrana larvae ranged from 64 to 91% at field conditions. Inhibition of E. microtheca growth reached 50% in comparison to the control. Conclusions Our study showed that M. anisopliae strains were highly effective in ensuring control of two different detrimental agents of V. vinifera L., providing new evidence to support the dual effects of entomopathogenic fungi.

scholarly journals Activity of strains of Metarhizium anisopliae (Metsch) Sorokin against white grub (Chiloloba acuta, Scarabaeidae, Coleoptera) under laboratory condition in Nepal

2018 ◽  
Vol 17 ◽  
pp. 82-91 ◽  
Author(s):  
Dipak Khanal
Keyword(s):  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Insect Pests ◽  
Microbial Control ◽  
White Grub ◽  
White Grubs ◽  
Larval Stages ◽  
Commercial Strain ◽  
Randomized Design ◽  
Completely Randomized Design

Soil insect pests are the major productivity constrains of different crops among which white grubs (Coleoptera: Scarabaeidae), both adult and larval stages, are extremely destructive in nature. Laboratory studies were conducted to evaluate the virulence of an indigenous and a commercial strain of the entomopathogenic fungi, Metarhizium anisopliae (Metsch.) Sorokin, against white grubs species Chiloloba acuta by applying the dipping method at Entomology Division, NARC, Nepal. Third instars larvae of C. acuta were dipped in suspensions of indigenous and commercial strains (Pacer) of M. anisopliae at different concentrations ranging from 3.33×104 to 1.04×108 spores/ml for 3-5 seconds which resulted in 97.8% and 89% mortalities with the highest dose of 1.04×108 spores/ml 40 days after inoculation (DAI), respectively. The LC50 values for indigenous and commercial strain were 3.5×105 and 1.88×106 spores/ml, respectively, with the potency of 1:0.28 at 40 DAI. Bioassays were conducted in completely randomized design. From results it is concluded that the tested strains of entomopathogenic fungi have potential as microbial control agents in managing white grubs in laboratory and it is suggested to be tested under farmers field condition.

scholarly journals Efficacy of strains of Metarhizium anisopliae (Metsch) Sorokin with their different preparations against white grub (Chiloloba acuta) in vitro in Nepal

2014 ◽  
Vol 15 ◽  
pp. 46-57
Author(s):  
Dipak Khanal ◽  
Yubak D GC ◽  
Yagya P Giri ◽  
Marc Sporleder ◽  
Resham B Thapa
Keyword(s):  
Entomopathogenic Fungi ◽  
Microbial Control ◽  
Barley Grain ◽  
Laboratory Studies ◽  
White Grub ◽  
White Grubs ◽  
Commercial Strain ◽  
Great Possibility ◽  
Relative Potencies

Laboratory studies were conducted to evaluate the virulence of an indigenous and a commercial strain of the entomopathogenic fungi, Metarhiziuma nisopliae (Metsch.) Sorokin in three different preparations against third instars larva of Chiloloba acuta.Conidia of the indigenous strain were multiplied on barley grain, ("Ind-G") while the commercial strain was tested in its powder ("Com-P") and solution ("Com-S") preparations. All fungus preparations were bioassayed at different concentrations levels; i.e. "Ind-G" at 60, 10, 1.66, 0.27, 0.046 g/ kg of soil, "Com-P" at 10, 1.66, 0.27, 0.046, 0.0077g/kg of soil and "Com-S" at 10, 1.66, 0.27, 0.046, 0.0077g /(100 ml water+ 1 kg soil). The LC50-value of Ind-G (0.41 g/kg of soil) was higher as compared to Com-P (0.11 g/kg of soil) and Com-S (0.10 g/kg of soil) revealing relative potencies of 1, 3.27 (CL 95% ranging 1.01- 10.29) and 4.11(CL 95% ranging 1.28 to13.01), respectively at 40 days after inoculation (DAI). Based on this study there is great possibility that the entomopathogenic fungi could be a safe microbial control agents in managing white grubs.

Virulence of Some Entomopathogenic Fungi Isolates of Beauveria bassiana (Hypocreales: Cordycipitaceae) and Metarhizium anisopliae (Hypocreales: Clavicipitaceae) to Aulacaspis tubercularis (Hemiptera: Diaspididae)and Icerya seychellarum (Hemiptera: Monophlebidae) on Mango Crop

2019 ◽  
Author(s):  
Atef M M Sayed ◽  
Christopher A Dunlap
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Insect Pests ◽  
Microbial Control ◽  
Probit Analysis ◽  
Scale Insects ◽  
Elapsed Time ◽  
Fungal Isolates ◽  
Further Development

Abstract Six fungal isolates of Beauveria bassiana (Balsamo) Vuillemin and one isolate of Metarhizium anisopliae (Metschnikoff) Sorokin were isolated and evaluated for their pathogenicity to Icerya seychellarum (Westwood) and Aulacaspis tubercularis Newstead. There is a positive correlation between the concentration of the fungal blastospore concentrations and the percentage of mortality. Bio-efficacy increased significantly after inoculation with increasing concentration of blastospores and elapsed time up to 12 d after inoculation. The mortality of nymphs exposed to fungal isolates at various concentrations varied between 2.5 and 88.8%. Probit analysis of data at 95% confidence limits of LC50 and LT50s showed significant differences in the susceptibility of nymphs of I. seychellarum and A. tubercularis to the tested fungal isolates. The fungal isolates of Egy-6 and Egy-9 were the most effective against I. seychellarum and A. tubercularis, respectively. They had the lowest LC50 (4.20 × 105 and 5.71 × 103 blastospore ml−1) and LT50 (ranged from 4.61 to 9.79 and 4.84 to 8.71 d), respectively. The current study showed that all the fungal isolates yielded moderate mortality rates of nymphs and adult female populations of both the tested insect pests. To our knowledge, this is the first report of bio-efficacy of Beauveria and Metarhizium isolates against members of the Diaspidadae and Monophlebidae family insects. These results establish that the use of these native entomopathogenic fungi isolates of B. bassiana (Egy-3, Egy-4, Egy-6, Egy-7, Egy-9, and Egy-10) and M. anisopliae (Egy-5) could be considered for further development as microbial control agents of the mealybug and scale insects as a potential biological agent for use in an IPM program.

scholarly journals Entomopathogenic fungi as biological controllers: New insights into their virulence and pathogenicity

2012 ◽  
Vol 64 (1) ◽  
pp. 21-42 ◽  
Author(s):  
Ali Shahid ◽  
Qayyum Rao ◽  
Allah Bakhsh ◽  
Tayyab Husnain
Keyword(s):  
Entomopathogenic Fungi ◽  
Extracellular Enzymes ◽  
Insect Pests ◽  
Microbial Control ◽  
Successful Infection ◽  
Fungal Entomopathogens ◽  
Penetration Ability ◽  
Insect Integument ◽  
Managed Ecosystems ◽  
Effective Use

Entomopathogenic fungi vary considerably in their mode of action and virulence. Successful infection depends primarily on the adherence and penetration ability of a fungus to the host integuments. A variety of extracellular enzymes is produced during the degradation of insect integument. The attempts to control insects have changed over time from chemicals to natural control methods. This is why the development of natural methods of insect control or biopesticides, is preferred. By the use of fungal entomopathogens, insect pests can be controlled. There is no doubt that insects have been used for many years, but their effective use in the field remains elusive. However, their additional role in nature has also been discovered. Comparison of entomopathogens with conventional chemical pesticides depends on their efficiency and cost. In addition to efficiency, there are advantages in using microbial control agents, such as human safety and other non-target organisms; pesticide residues are minimized in food and biodiversity increased in managed ecosystems. In the present review the pathogenicity and virulence of entomopathogenic fungi and their role as biological control agents using biotechnology will be discussed.

scholarly journals Microbial Control of Invasive Forest Pests with Entomopathogenic Fungi: A Review of the Current Situation

Insects ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 341 ◽  
Author(s):  
Surendra K. Dara ◽  
Cristian Montalva ◽  
Marek Barta
Keyword(s):  
Entomopathogenic Fungi ◽  
Insect Pests ◽  
Microbial Control ◽  
Environmental Implications ◽  
Forest Pests ◽  
The North ◽  
The World ◽  
Forest Pest Management ◽  
The Status ◽  
Cypress Aphid

The health of the forestlands of the world is impacted by a number of insect pests and some of them cause significant damage with serious economic and environmental implications. Whether it is damage of the North American cypress aphid in South America and Africa, or the destruction of maple trees in North America by the Asian long horned beetle, invasive forest pests are a major problem in many parts of the world. Several studies explored microbial control opportunities of invasive forest pests with entomopathogenic bacteria, fungi, and viruses, and some are successfully utilized as a part of integrated forest pest management programs around the world. This manuscript discusses some invasive pests and the status of their microbial control around the world with entomopathogenic fungi.

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