Serum levels of proteins and acute-phase proteins in captive emus ( Dromaius novaehllandieae ) of different ages

ABSTRACT Protein electrophoresis is a relatively simple technique that allows separating serum protein fractions, and provides important information in the investigation and diagnosis of several diseases. This study determined the levels of acute-phase proteins in the serum of healthy, captive emus (Dromaius novaehollandiae). Animals were divided into two groups (n=11 in each) based on age, with 1-year-old and 4-year-old emus. Acute-phase proteins were separated by SDS-PAGE. Ceruloplasmin, transferrin, albumin, haptoglobin, acidic glycoprotein, IgA, and IgG were detected in the serum of all animals. Protein profiles varied significantly with age (P<0.05). Individuals in the 4-year-old emus group had higher values of ceruloplasmin, transferrin, albumin, haptoglobin, and acidic glycoprotein, compared with the group with 1-year-old animals, showing the role of age in the protein profile of this species. Reference values for acute-phase proteins in healthy emus may be useful in the evaluation of health status and in the diagnosis of diseases affecting the species.

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The role of alpha-2-macroglobulin in pathogenesis of rheumatoid arthritis and system lupus erythematosus

Bulletin of Siberian Medicine ◽

10.20538/1682-0363-2010-5-39-44 ◽

2010 ◽

Vol 9 (5) ◽

pp. 39-44

Author(s):

V. N. Zorina ◽

I. G. Kozlov ◽

R. M. Zorina ◽

N. A. Trofimenko ◽

T. S. Chirikova ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Disease Progression ◽

Acute Phase ◽

Lupus Erythematosus ◽

Acute Phase Proteins ◽

Serum Levels ◽

Control Group ◽

Healthy Donors ◽

Significant Difference

We investigated serum levels of alpha-2-macroglobulin (α2—MG) and some of its complexes, namely α2-MG-plasmin (α2-MG— Pl) and α2-MG—IgG at a rheumatoid arthritis (RA) 2—3 degrees of activity (65 patients), a system lupus erythematosus (SLE) of 2-3 degrees of activity (30 patients) and 55 healthy donors as a control group. It is shown, that at SLE the total level of α2-MG is invariable, and at RA — decreases significantly in comparison with the healthy. The concentration of complexes was raised at pathology, but at RA this rising was expressed much more strongly, than at SLE. At studying of correlations of levels of α2-MG, α2-MG—Pl and α2-MG— IgG among themselves and with some several cytokines and acute phase proteins, it is shown, that there is some significant difference between normal and pathological correlative relations and allows us to suspect that at SLE, the α2-MG and its complexes participate in a pathogenesis, and at RA α2-MG becomes the major immunogenesis factor and the significant reason of disease progression.

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Acute Phase Proteins and Interleukin?6 Serum Levels in Patients with Chronic Arterial Occlusion

Annals of the New York Academy of Sciences ◽

10.1111/j.1749-6632.1995.tb32379.x ◽

2006 ◽

Vol 762 (1) ◽

pp. 493-495 ◽

Cited By ~ 1

Author(s):

WACLAW MAJEWSKI ◽

RYSZARD STANISZEWSKI ◽

ARTUR SLUPIANEK ◽

ALEKSANDER GORNY ◽

ANDRZEJ MACKIEWICZ

Keyword(s):

Acute Phase ◽

Interleukin 6 ◽

Acute Phase Proteins ◽

Arterial Occlusion ◽

Serum Levels ◽

Chronic Arterial Occlusion

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Establishment of peritoneal liquid electrophoretogram from healthy horses and horses submitted to experimentally induced intestinal obstruction

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-41625993 ◽

2014 ◽

Vol 66 (3) ◽

pp. 665-671 ◽

Cited By ~ 1

Author(s):

A.F.S. Nogueira ◽

P.A. Di Filippo ◽

L.A. Anai ◽

M.C. Vieira ◽

K.M.M.G. Simplício ◽

...

Keyword(s):

Intestinal Obstruction ◽

Acute Phase ◽

Acute Phase Proteins ◽

Immunoglobulin A ◽

Control Group ◽

Protein Levels ◽

Sds Page ◽

Significant Difference ◽

Page Electrophoresis ◽

Experimentally Induced

The initial inflammatory stages of the colic syndrome include changes known as acute phase response. The aim of this study was to contribute with the establishment of reference values concerning the electrophoretogram of peritoneal liquid from healthy horses and horses submitted to experimentally induced intestinal obstruction. Twenty-one horses were allotted in four groups: duodenal obstruction (DG), ileum obstruction (IG), left-dorsal colon obstruction (MG), and control group (CG). Peritoneal liquid was sampled before obtruction (T0), with 3 hours of obstruction (T3) and 6, 30, 102 and 174 hours after desobstructing (T6, T30, T102 and T174, respectively). Total protein levels were determined by the biuret method and protein fractions were obtained by SDS-PAGE electrophoresis. The acute phase proteins (APP) identified were Immunoglobulin-A, ceruloplasmin, transferrin, albumin, α1-antitrypsin, heavy and light chains of immunoglobulin-G, haptoglobin, α1-acid glycoprotein and a still unnamed protein, which was called P24. There was no difference (P>0.3) in protein levels among groups, although a significant difference (P>0.05) was observed between distinct experimental moments in each group evidencing a higher response of the APP in the obstructed groups. The APP fractioning of the peritoneal liquid was standardized to establish a standard curve for healthy equines and those submitted to induced intestinal obstruction. Moreover, it was verified that the SDS-PAGE electrophoresis was sensitive and effective to help diagnose abdominal inflammatory processes.

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Abstract 1629: Genetic Polymorphism A1675G On Angiotensin Receptor Type 2, Is Implicated In The Expression Of Acute Phase Proteins And The Development Of Coronary Atherosclerosis In Hypertensive Patients

Circulation ◽

10.1161/circ.116.suppl_16.ii_341-b ◽

2007 ◽

Vol 116 (suppl_16) ◽

Author(s):

Dimitris Tousoulis ◽

Nikolas Koumallos ◽

Charalambos Antoniades ◽

Despina Kardara ◽

Alexis S Antonopoulos ◽

...

Keyword(s):

Genetic Polymorphism ◽

Arterial Hypertension ◽

Acute Phase ◽

Coronary Atherosclerosis ◽

Acute Phase Proteins ◽

Renin Angiotensin System ◽

Serum Levels ◽

Hypertensive Patients ◽

The Impact

Introduction: Renin-angiotensin system affects cardiovascular disease pathogenesis through a balance of angiotensin II effects on proatherogenic constitutive type 1 and antiatherogenic inducible type 2 (AT2R) receptors. The impact of A1675G polymorphism on the development of hypertension and advanced atherosclerosis is controversial. We examined the impact of A1675G polymorphism on AT2R, on the risk for arterial hypertension and coronary atherosclerosis, and its effect on the expression of proatherogenic inflammatory molecules. Methods. The study population consisted of 310 males: 145 with arterial hypertension and 165 controls, matched for age and risk factors for atherosclerosis. Among hypertensive subjects, 37 had angiographically documented coronary atherosclerosis and 108 had no evidence of atherosclerosis. The presence of A1675G polymorphism on AT2R gene (located in chromosome X) was determined by PCR. Serum levels of C-reactive protein and fibrinogen was measured in all the participants. Results. The frequency of the A allele was similar between patients with arterial hypertension (64/145, 44.1%) and non-hypertensive subjects (73/165, 44.2%, p=NS), while the risk for arterial hypertension was OR[95%CI]:1.004[0.641–1.574], p=0.985 for the G vs A carriers. However, the risk for coronary atherosclerosis within the group of hypertensive subjects was significantly elevated in the carriers of the A allele (OR[95%CI]:2.128[1.003–4.513], p=0.04 vs carriers of the G allele). Importantly, the presence of the A allele was also associated with significantly higher levels of CRP (4.8±0.8mg/dl) compared to the carriers of the G allele (3.0±0.3mg/dl, p<0.05). Similarly, fibrinogen levels were higher in A-allele carriers (median(25 th –75th percentile) 395(340 – 455) mg/ml) compared to G-allele carriers (369(320 – 406) mg/ml, p<0.05). Conclusions: Although genetic polymorphism A1675G on AT2R is not associated with the development of arterial hypertension, it affects the risk for coronary artery disease among hypertensive patients. The presence of the A allele also leads to higher levels of CRP and fibrinogen, implying that this polymorphism may induce atherogenesis by modulating acute phase response in hypertensive individuals.

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The Role of Acute Phase Proteins in Induction of Tension of Nonspecific Resistance System in Various Clinical Phenotypes of Myasthenia

Journal of Clinical & Experimental Pathology ◽

10.4172/2161-0681.1000300 ◽

2016 ◽

Vol 06 (06) ◽

Author(s):

Klimova EM ◽

Kalashnikova JV

Keyword(s):

Acute Phase ◽

Acute Phase Proteins ◽

Nonspecific Resistance ◽

Clinical Phenotypes ◽

Resistance System

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Role of prostaglandins in acute phase proteins in inflammation

Biochemical Medicine ◽

10.1016/0006-2944(84)90028-0 ◽

1984 ◽

Vol 31 (2) ◽

pp. 236-245 ◽

Cited By ~ 14

Author(s):

K. Anbalagan ◽

J. Sadique

Keyword(s):

Acute Phase ◽

Acute Phase Proteins

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Adipokines: inflammation and the pleiotropic role of white adipose tissue

British Journal Of Nutrition ◽

10.1079/bjn20041213 ◽

2004 ◽

Vol 92 (3) ◽

pp. 347-355 ◽

Cited By ~ 1305

Author(s):

Paul Trayhurn ◽

I. Stuart Wood

Keyword(s):

Adipose Tissue ◽

Growth Factor ◽

Inflammatory Cytokines ◽

Acute Phase ◽

White Adipose Tissue ◽

Acute Phase Proteins ◽

Endocrine Function ◽

Low Grade ◽

Circulating Levels

White adipose tissue is now recognised to be a multifunctional organ; in addition to the central role of lipid storage, it has a major endocrine function secreting several hormones, notably leptin and adiponectin, and a diverse range of other protein factors. These various protein signals have been given the collective name ‘adipocytokines’ or ‘adipokines’. However, since most are neither ‘cytokines’ nor ‘cytokine-like’, it is recommended that the term ‘adipokine’ be universally adopted to describe a protein that is secreted from (and synthesised by) adipocytes. It is suggested that the term is restricted to proteins secreted from adipocytes, excluding signals released only by the other cell types (such as macrophages) in adipose tissue. Theadipokinome(which together with lipid moieties released, such as fatty acids and prostaglandins, constitute thesecretomeof fat cells) includes proteins involved in lipid metabolism, insulin sensitivity, the alternative complement system, vascular haemostasis, blood pressure regulation and angiogenesis, as well as the regulation of energy balance. In addition, there is a growing list of adipokines involved in inflammation (TNFα, IL-1β, IL-6, IL-8, IL-10, transforming growth factor-β, nerve growth factor) and the acute-phase response (plasminogen activator inhibitor-1, haptoglobin, serum amyloid A). Production of these proteins by adipose tissue is increased in obesity, and raised circulating levels of several acute-phase proteins and inflammatory cytokines has led to the view that the obese are characterised by a state of chronic low-grade inflammation, and that this links causally to insulin resistance and the metabolic syndrome. It is, however, unclear as to the extent to which adipose tissue contributes quantitatively to the elevated circulating levels of these factors in obesity and whether there is a generalised or local state of inflammation. The parsimonious view is that the increased production of inflammatory cytokines and acute-phase proteins by adipose tissue in obesity relates primarily to localised events within the expanding fat depots. It is suggested that these events reflect hypoxia in parts of the growing adipose tissue mass in advance of angiogenesis, and involve the key controller of the cellular response to hypoxia, the transcription factor hypoxia inducible factor-1.

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Concentrations of acute-phase proteins and immunoglobulins in serum and synovial fluid in clinically healthy heifers and steers

Pesquisa Veterinária Brasileira ◽

10.1590/1678-5150-pvb-5817 ◽

2019 ◽

Vol 39 (6) ◽

pp. 388-392

Author(s):

Paula A. Di Filippo ◽

Saulo T. Lannes ◽

Marcos A.D. Meireles ◽

Andressa F.S. Nogueira ◽

Célia R. Quirino

Keyword(s):

Synovial Fluid ◽

Acute Phase ◽

Acute Phase Proteins ◽

Immunoglobulin A ◽

Crossbred Cattle ◽

Acid Glycoprotein ◽

Molecular Weights ◽

Sds Page ◽

Radiocarpal Joint ◽

Statistical Program

ABSTRACT: The aim of the study was to determine the concentration pattern of intra-articular acute phase proteins (APPs) and immunoglobulins in healthy crossbred cattle. Synovial fluid (SF) samples were collected from the radiocarpal joint of 25 heifers and 25 steers. Concentrations of APPs were measured by SDS-PAGE. The results were submitted to analysis of variance using the SAS statistical program, and means were compared by the Student-Newman-Keuls test (P<0.05). Thirty-seven proteins with molecular weights ranging from 7 to 37kDa were identified in SF of all animals. Eight were nominally identified with immunoglobulin A (IgA) and G (IgG), ceruloplasmin (Cp), transferrin (Tf), albumin (Ab), α1-antitripsin (AAT), α1-acid glycoprotein (AGP), and haptoglobin (Hp). The α1-antitripsin was only identified in the Sf of the heifers. The SF values of Cp, Hp, AGP and IgA were significantly higher in heifers than in steers. In sera, 34 proteins with molecular weights between 7 and 244kDa were identified in heifers and steers. Similar proteins were nominally identified in the sera, however the α1-antitrypsin was identified only in SF. The serum values Tf, AGP and IgG were significantly higher in heifers compared with steers. In conclusion, the physiological acute-phase proteins concentrations in synovial fluid of healthy ruminants can be useful in the interpretation of samples from animals with joint diseases. The SF electrophoretic profile of healthy ruminants differs depending on gender. Similar proteins were nominally identified in the sera, but only the SF of α1-antitrypsin.

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