The new bone formation in human maxillary sinuses using two bone substitutes with different resorption types associated or not with autogenous bone graft: a comparative histomorphometric, immunohistochemical and randomized clinical study

Introduction. Autogenous bone graft is the gold standard in reconstruction of bone defects. The use of autogenous bone graft is problematic because of limited bone as well as donor site morbidity. This study evaluates a novel biomaterial as an alternative to autogenous bone graft. The biomaterial is amniotic membrane, rich in growth factors. Methods. Twenty-one adult male Sprague-Dawley rats were implanted with biomaterial using the rat critical size femoral gap model. After creation of the critical size femoral gap animals were randomized to one of the following groups: Group 1 (control): gap left empty and received no treatment; Group 2 (experimental): the gap was filled with commercially available bone graft; Group 3 (experimental): the gap was filled with bone graft plus NuCel amniotic tissue preparation. Results. The experimental groups demonstrated new bone formation compared to controls. The results were evident on radiographs and histology. Histology showed Group 1 controls to have 11.1% new bone formation, 37.8% for Group 2, and 49.2% for Group 3. These results were statistically significant. Conclusions. The study demonstrates that amniotic membrane products have potential to provide bridging of bone defects. Filling bone defects without harvesting autogenous bone would provide a significant improvement in patient care.

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Autogenous Bone Graft and Bone Substitutes

Journal of Korean Society of Spine Surgery ◽

10.4184/jkss.2008.15.1.54 ◽

2008 ◽

Vol 15 (1) ◽

pp. 54 ◽

Cited By ~ 2

Author(s):

Dong-Jun Kim

Keyword(s):

Bone Graft ◽

Bone Substitutes ◽

Autogenous Bone Graft ◽

Autogenous Bone

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Bone Formation in Maxillary Sinus Lift Using Autogenous Bone Graft at 2 and 6 Months

International Journal of Morphology ◽

10.4067/s0717-95022016000300041 ◽

2016 ◽

Vol 34 (3) ◽

pp. 1069-1075 ◽

Cited By ~ 1

Author(s):

Henrique Duque Netto ◽

Maria das Graças Alfonso Miranda Chaves ◽

Beatriz Aatrstrup ◽

Renata Guerra ◽

Sergio Olate

Keyword(s):

Bone Formation ◽

Bone Graft ◽

Maxillary Sinus ◽

Autogenous Bone Graft ◽

Autogenous Bone ◽

Sinus Lift

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Histomorphometric, radiographic and biomechanical analyses of platelet rich plasma associated with autogenous bone graft

Brazilian Dental Science ◽

10.14295/bds.2014.v17i4.998 ◽

2014 ◽

Vol 17 (4) ◽

pp. 53 ◽

Cited By ~ 1

Author(s):

Adriana Socorro Ferreira Monteiro ◽

Nelson Luiz Macedo ◽

Luiz Guilherme Scavone Macedo ◽

Rosilene Fernandes Rocha

Keyword(s):

Bone Formation ◽

Bone Graft ◽

Bone Defect ◽

Optical Density ◽

Platelet Rich Plasma ◽

Biomechanical Analysis ◽

Autogenous Bone Graft ◽

Radiographic Evaluation ◽

Autogenous Bone ◽

Autogenous Graft

Objective: The aim of this study was to evaluate the effectiveness of platelet rich plasma with and without autogenous bone graft in the bone repair of surgical defects in rabbit tibias. Materials and Methods: In this research, 25 adult male rabbits were used. Two defects have been performed in each tibia, divided into four groups: control (C = defect naturally left to heal by clot formation), autogenous (A = bone defect + autogenous graft), PRP (PRP = bone defect + PRP) and autogenous + PRP (PRPA = bone defect + autogenous graft + PRP). All the defects were covered with a dPTFE membrane. Five other animals were sacrificed at 15, 30, and 60-day postoperatively. The pieces containing the defects were processed for histological and histomorphometric analysis. Other five animals were sacrificed after 30 and 60 days and submitted to biomechanical analysis, and all the specimens were sent to the radiographic evaluation of optical density. Results: The biomechanical, radiographic, and histomorphometric results showed larger resistance, optical density, and improving bone formation in the groups A and PRPA when compared with the groups C and PRP. Conclusion: This study showed there was not an improvement in the radiographic, mechanical, and bone formation parameters when PRP was used individually or associated to the autogenous bone graft.

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Histomorphology on Healing of the Chitosan Membrane and β-TCP on Dental Implant Dehiscence Defects in Dogs

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.309-311.255 ◽

2006 ◽

Vol 309-311 ◽

pp. 255-258

Author(s):

Y.S. Kim ◽

Tae Gyun Kim ◽

Ui Won Jung ◽

C.S. Kim ◽

Seong Ho Choi ◽

...

Keyword(s):

Bone Formation ◽

Alveolar Bone ◽

Bone Substitutes ◽

Bone Defects ◽

Autogenous Bone ◽

Control Group ◽

New Bone Formation ◽

Chitosan Membrane ◽

Space Maintenance ◽

Experimental Group

Dehiscence bone defects, frequently observed on dental implants placed in periodontitis-affected alveolar bone or extraction sockets were treated with β-tricalcium phosphate (β –TCP) and chitosan membrane for guided bone regeneration, and the new bone formation on the treated sites were studied. Beagle dogs were used for the experiment. First to fourth mandibular premolars were extracted, and the post extraction alveolar bone surface was planed. After 8 weeks of healing, 3 by 4mm dehiscence defects were created using straight fissure burs. Total of 16 oxidized titanium surface implants were placed on the bone defects of the subjects, two on each side. Control sites were treated with implants only. Experimental Group 1 sites were treated with implants and chitosan membrane. Experimental Group 2 sites were treated with implants, β-TCP and chitosan membrane. Experimental Group 3 sites were treated with implants, β-TCP, autogenous bone and chitosan membrane. The animals were sacrificed 12 weeks after implant placement, and the specimens from the treated sites were histologically studied with following results. Limited amount of new bone formation was observed in control group with unexposed membrane. Slightly greater amount of bone formation was observed on sites treated with β-TCP+membrane or autogenous bone+ β-TCP+membrane compared to control group. Remnants of chitosan membrane and β-TCP encapsulated with connective tissue were observed during experimental periods. These results suggest that further studies are needed on membrane rigidity and infection control for space maintenance underneath the membrane and bone substitutes in the treatment of dehiscence defects.

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Influence of biodegradable polymer membrane on new bone formation and biodegradation of biphasic bone substitutes: an animal mandibular defect model study

Maxillofacial Plastic and Reconstructive Surgery ◽

10.1186/s40902-020-00280-5 ◽

2020 ◽

Vol 42 (1) ◽

Author(s):

Jeong-Kui Ku ◽

Young-Kyun Kim ◽

Pil-Young Yun

Keyword(s):

Bone Formation ◽

Bone Graft ◽

Bone Substitutes ◽

Bony Defect ◽

Graft Material ◽

New Bone Formation ◽

Bone Graft Material ◽

Barrier Membrane ◽

Model Study ◽

Significant Difference

Abstract Purpose The purpose of this study was to evaluate the influence of biodegradable polycaprolactone membrane on new bone formation and the biodegradation of biphasic alloplastic bone substitutes using animal models. Materials and methods In this study, bony defect was formed at the canine mandible of 8 mm in diameter, and the defects were filled with Osteon II. The experimental groups were covered with Osteoguide as barrier membrane, and the control groups were closed without membrane coverage. The proportion of new bone and residual bone graft material was measured histologically and histomorphometrically at postoperative 4 and 8 weeks. Results At 4 weeks, the new bone proportion was similar between the groups. The proportion of remaining graft volume was 27.58 ± 6.26 and 20.01 ± 4.68% on control and experimental groups, respectively (P < 0.05). There was no significant difference between the two groups in new bone formation and the amount of residual bone graft material at 8 weeks. Conclusion The biopolymer membrane contributes to early biodegradation of biphasic bone substitutes in the jaw defect but it does not affect the bone formation capacity of the bone graft.

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Poster Board Number: 09: A Comparison of Allogenous Bone Graft Versus Particulate Autogenous Bone Graft to Gain Increase in Vertical and Horizontal Dimension Prior to Implant Placement—A Human Clinical Study

Journal of Oral and Maxillofacial Surgery ◽

10.1016/j.joms.2010.06.092 ◽

2010 ◽

Vol 68 (9) ◽

pp. e64-e65

Author(s):

S.E. Kelly

Keyword(s):

Clinical Study ◽

Bone Graft ◽

Autogenous Bone Graft ◽

Autogenous Bone ◽

Horizontal Dimension ◽

Implant Placement ◽

Human Clinical Study

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Treatment of class II furcation defects with autogenous bone graft associated with Bichat’s fat pad: case report

RSBO ◽

10.21726/rsbo.v1i2.412 ◽

2017 ◽

Vol 1 (2) ◽

pp. 114

Author(s):

Priscila Alves Teixeira ◽

Carmen L. Mueller Storrer ◽

Felipe Rychuv Santos ◽

Aline Monise Sebastiani ◽

Tatiana Miranda Deliberador

Keyword(s):

Case Report ◽

Bone Graft ◽

Low Cost ◽

Class Ii ◽

Autogenous Bone Graft ◽

Surgical Reconstruction ◽

Autogenous Bone ◽

Fat Pad ◽

Furcation Defects ◽

Maxillary Tuberosity

The periodontal treatment of teeth with furcation defect is clinically challenging. In cases of class II furcation defects, the regenerative surgery shows low morbidity and good prognosis when correctly indicated. The aim of the presentstudy is to report a treatment option for class II furcation defect through autogenous bone graft associated with the Bichat’s fat pad. Case report: A 59-year-old female patient was diagnosed with class II furcation defect in the left mandibular first molar. The treatment comprised surgical reconstruction of the defect with a combination of maxillary tuberosity bone graft and Bichat’s fat pad. The clinical and radiographic follow-up of 180 days showed bone formation inthe furcation area and absence of probing depth. Conclusion: An association of autogenous graft form the maxillary tuberosity with a Bichat’s fat pad proved to be a safe, low cost, and effective therapy for the regenerative treatment of class II furcation.

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Mechanistic Illustration: How Newly-Formed Blood Vessels Stopped by the Mineral Blocks of Bone Substitutes Can Be Avoided by Using Innovative Combined Therapeutics

Biomedicines ◽

10.3390/biomedicines9080952 ◽

2021 ◽

Vol 9 (8) ◽

pp. 952

Author(s):

Fabien Bornert ◽

François Clauss ◽

Guoqiang Hua ◽

Ysia Idoux-Gillet ◽

Laetitia Keller ◽

...

Keyword(s):

Regenerative Medicine ◽

Bone Formation ◽

Medicinal Product ◽

Blood Vessels ◽

Bone Substitute ◽

Bone Substitutes ◽

Bone Defects ◽

New Bone Formation ◽

Polymeric Component ◽

Bone Filling

One major limitation for the vascularization of bone substitutes used for filling is the presence of mineral blocks. The newly-formed blood vessels are stopped or have to circumvent the mineral blocks, resulting in inefficient delivery of oxygen and nutrients to the implant. This leads to necrosis within the implant and to poor engraftment of the bone substitute. The aim of the present study is to provide a bone substitute currently used in the clinic with suitably guided vascularization properties. This therapeutic hybrid bone filling, containing a mineral and a polymeric component, is fortified with pro-angiogenic smart nano-therapeutics that allow the release of angiogenic molecules. Our data showed that the improved vasculature within the implant promoted new bone formation and that the newly-formed bone swapped the mineral blocks of the bone substitutes much more efficiently than in non-functionalized bone substitutes. Therefore, we demonstrated that our therapeutic bone substitute is an advanced therapeutical medicinal product, with great potential to recuperate and guide vascularization that is stopped by mineral blocks, and can improve the regeneration of critical-sized bone defects. We have also elucidated the mechanism to understand how the newly-formed vessels can no longer encounter mineral blocks and pursue their course of vasculature, giving our advanced therapeutical bone filling great potential to be used in many applications, by combining filling and nano-regenerative medicine that currently fall short because of problems related to the lack of oxygen and nutrients.

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