scholarly journals PHYSIOLOGICAL QUALITY AND ANTIOXIDANT ENZYMATIC ACTION IN SUNFLOWER SEEDS EXPOSED TO DETERIORATION1

2021 ◽  
Vol 34 (3) ◽  
pp. 570-579
Author(s):  
THAÍS DE CASTRO MORAIS ◽  
DENISE CUNHA FERNANDES DOS SANTOS DIAS ◽  
DANIEL TEIXEIRA PINHEIRO ◽  
GUILHERME FONTES VALORY GAMA ◽  
LAÉRCIO JUNIO DA SILVA
Keyword(s):  
Accelerated Aging ◽  
Sunflower Seeds ◽  
Sod Activity ◽  
Test Methodology ◽  
Accelerated Aging Test ◽  
Seedling Length ◽  
Aging Test ◽  
Physiological Quality ◽  
Controlled Deterioration ◽  
Peroxidase Enzymes

ABSTRACT This work aimed to evaluate the physiological and biochemical changes resulting from controlled deterioration in different lots of sunflower seeds. Two sunflower seed lots of the cultivar Hélio 253 were subjected to simulated deterioration, using the accelerated aging test methodology (41 °C and 100% RH) for 0, 48, 72, and 96 h. Then, the seeds were subjected to the tests of germination, first germination count, seedling length and dry matter, emergence, accelerated aging, electrical conductivity, and total seedling length. The antioxidant activity was evaluated through the enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX), and peroxidase (POX) at 0, 2, 4, and 6 days after sowing. Deterioration caused a reduction in the germination and vigor of the seeds, mainly in the lot with lower initial vigor and for the longest exposure times (72 and 96 h). For both lots, reductions in SOD activity and increases in POX and APX were observed during seed germination, mainly after 48 h of exposure to aging deterioration. Peroxidase enzymes are activated in deteriorated and non-deteriorated sunflower seeds, mainly after two days of germination.

scholarly journals Genetic diversity for physiological quality of seeds from corn (Zea mays L.) intervarietal crossbreeds

2013 ◽  
Vol 35 (4) ◽  
pp. 449-456 ◽  
Author(s):  
Daniele Nerling ◽  
Cileide Maria Medeiros Coelho ◽  
Rubens Onofre Nodari
Keyword(s):  
Genetic Diversity ◽  
Zea Mays L ◽  
Accelerated Aging ◽  
Genetic Contribution ◽  
Percentage Germination ◽  
Canonical Variables ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality

The physiological quality is based on the genotype and may be accompanied since the first stages of selection through the study of genetic diversity. The purpose of this study was to evaluate the parental genetic contribution in the seed physiological quality in corn intervarietal crossbreeds. The intervarietal crossbreeds were performed using landraces genotypes, open pollinated varieties and commercial hybrids. The seeds obtained from the crossbreed, their equivalents and parentals were evaluated regarding their water percentage, germination, germination speed rate, accelerated aging, field emergence and electrical conductivity. The seeds obtained in the crossbreed are viable and vivid with heterosis when compared with the parentals. The accelerated aging test was more effective for selecting the strongest genotypes. The canonical variables were useful to group the genotypes that presented similar physiological characteristics. The genotypes MPA 01 x Pixurum 05, MPA 01 x AS1565, MPA 01 x SJC5886, SJC5886 x Pixurum 05 and Fundacep 35 x SCS 154 Fortuna were the best crossbreeds regarding the production of better physiological quality seeds.

Reduction of the Forage Seeds Physiological Quality by the Contact with Fertilizer in the Crop-Livestock Integration

2019 ◽  
pp. 1-11
Author(s):  
Anne Caroline Dallabrida Avelino ◽  
Dayana Aparecida De Faria ◽  
Carlos Eduardo Avelino Cabral ◽  
Joadil Gonçalves De Abreu ◽  
Elisangela Clarete Camili ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Contact Time ◽  
Accelerated Aging ◽  
Germination Percentage ◽  
Randomized Design ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality ◽  
Completely Randomized Design ◽  
Seeds Germination

The aim of this study was to evaluate the effects of Brachiaria seed contact time with 05-25-15 fertilizer (N-P2O5-K2O) on the seed’s physiological quality. The experimental design was a completely randomized design with four replicates. Treatments were arranged in a 5x4 factorial, being: five seeds contact times with fertilizer (0, 24, 48, 72 and 96 hours); four forages: Marandu palisadegrass, Piatã palisadegrass, Xaraés palisadegrass and Ruziziensis grass. The performed tests were: water content, germination (first count and germination percentage), tetrazolium (viability), electrical conductivity, accelerated aging, sand emergency and emergence speed index. Contact time of 05-25-15 fertilizer with Brachiaria seeds reduces linearly: Germination, emergence and emergence speed index of Brachiaria seeds; Piatã and Xaraés palisadegrass seeds viability; vigor (electrical conductivity), except in Ruziziensis grass seeds; and seeds germination of Piatã palisadegrass submitted to accelerated aging test (vigor). Piatã palisadegrass is the least recommended, among the analyzed grasses, for intercropping in simultaneous sowing.

scholarly journals Accelerated Aging Test to Determine the Vigor of Mungbean Seeds

2019 ◽  
Vol 11 (7) ◽  
pp. 42
Author(s):  
Luciana Maria da Silva ◽  
Anderson Dias Vaz de Souza ◽  
Rogério Lámim Silva Junior ◽  
Natália Arruda ◽  
Nei Peixoto ◽  
...  
Keyword(s):  
Seed Quality ◽  
Germination Rate ◽  
Accelerated Aging ◽  
Dry Weight ◽  
Precise Determination ◽  
Germination Test ◽  
Accelerated Aging Test ◽  
Seedling Length ◽  
Aging Test ◽  
Randomized Experimental Design

The use of good quality seeds is fundamental for proper establishment of a crop. In this way, for more precise determination of seed quality, vigor tests are performed in addition to the germination test. These tests enable the selection of the best lots for commercialization and planting. This study evaluates the effects of temperature and exposure times of the accelerated aging test for vigor classification of mungbean seed lots. Seeds of the mungbean cultivar Esmeralda were used, being obtained from four cultivated plots at the State University of Goiás (UEG), Ipameri Câmpus, in the 2013, 2015, 2016, and 2017 harvests. The lots were initially characterized using germination and vigor tests (first germination count, germination rate index, seedling length, and seedling fresh and dry weight). The accelerated aging test was conducted with a completely randomized experimental design, in a 2 × 4 factorial arrangement consisting of two temperatures (40 and 42 °C) and four times (24, 48, 72, and 96 hours), with four replicates of 50 seeds per lot. The lots showed significant differences in the germination test, first germination count, germination rate index, and seedling length. The accelerated aging test was efficient in classifying lots. Lot 3 obtained the best results, while lot 1 obtained the lowest ones. The combination of 42 °C temperature and 72 h of seed exposure to the accelerated aging test is the best to classify mungbean seed lots.

scholarly journals Tests for evaluating the physiological quality of pitaya seeds

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4047
Author(s):  
Thiago Alberto Ortiz ◽  
Aline Moritz ◽  
Lúcia Sadayo Assari Takahashi ◽  
Mariana Ragassi Urbano
Keyword(s):  
Electrical Conductivity ◽  
Pearson Correlation ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Water Volume ◽  
Germination Test ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.

scholarly journals Improving the method for determining the physiological and sanitary potential of Gherkin seeds

2021 ◽  
Vol 37 ◽  
pp. e37087
Author(s):  
Hugo Cesar Rodrigues Moreira Catão ◽  
Ítala Menegon Castilho ◽  
Franciele Caixeta ◽  
Nilvanira Donizete Tebaldi ◽  
Pâmela Gomes Nakada Freitas
Keyword(s):  
Water Content ◽  
Saline Solution ◽  
Seed Quality ◽  
Accelerated Aging ◽  
Analysis Method ◽  
Germination Test ◽  
Seed Technology ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality

Gherkin seeds usually show irregular physiological quality. Seed production requires fast and reliable tests to evaluate seed quality. Germination test is considered a recognized analysis method; however, seed technology has pursuit the improvement of vigor tests aiming the evaluation of seed’s physiological potential. Thus, the objective of this work was to evaluate procedures to perform the test of accelerated aging and determine the physiological and sanitary potential of gherkin seeds. Four seed lots of cultivar Liso Calcuta were used in the study. To evaluate the initial physiological quality the water content was determined and germination and emergence tests, as well as indices of germination speed and emergence speed were used. The accelerated aging test was performed as traditionally and with saturated saline solution, with 48, 72 and 96 hours, at temperatures of 41oC and 45oC. After aging, the water content was determined, and seeds’ germination and sanity tests were performed. The experiment was set under a completely random design in factorial 4x3x2 (lots x aging periods x temperatures). The standard accelerated aging test and the test with saturated saline solution at 41oC for 96 hours were efficient to evaluate the vigor of gherkin seeds. Saturated saline solution provides uniform water absorption and deterioration in gherkin seeds, allowing to discriminate seed lots in different vigor levels. The salinity test after accelerated aging with saline solution reduces the incidence of some fungi.

scholarly journals QUALIDADE FISIOLÓGICA DE SEMENTES DE SOJA SUBMETIDAS À DESSECAÇÃO EM PRÉ-COLHEITA

2018 ◽  
Vol 14 (4) ◽  
pp. 112-120
Author(s):  
Renan Canzi Comin ◽  
Geri Eduardo Meneghello ◽  
Jader Job Franco ◽  
Alexandre Moscarelli Levien ◽  
Kassiana Kehl ◽  
...  
Keyword(s):  
Seed Quality ◽  
Accelerated Aging ◽  
Glufosinate Ammonium ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality ◽  
Important Idea ◽  
The City ◽  
Technical Recommendations

The use of high quality soybean seeds (Glycine maxL.) is one of the most important aspects to obtain satisfactory productivity indexes. The desiccation practice is an important idea of the producers to anticipate theharvest and to minimize the rapiddeterioration of seed quality in the field. In this context, the objective of this work was to evaluate the effect of the use of desiccants applied in R7stageon the physiological quality of soybean seeds.The experiment was conducted in experimental area, located in the city of Passo Fundo, Rio Grande do Sul. Four soybean cultivars (FPS Jupiter RR, FPS Paranapanema RR, BRS Tordilha RR and FPS Urano RR) were planted under experimental design, managed according to technical recommendations for the crop.Upon reaching the R7 stage, different desiccation treatments (without desiccation, paraquat, diquat and glufosinate ammonium) were applied to the subplot.The seeds were harvested with a plot harvester and the weight of one thousand seeds, germination test and accelerated aging test were evaluated, being the last two evaluated at 30 and 180 days after harvest. The pre-harvest desiccation at the R7 stage of soybean cultivation did not negatively affect the physiological quality of the seeds and, generally way, for the cultivars FPS Jupiter RR and FPS Urano RR, it was possible to obtain more vigorous seeds at 30 DAC. Under the conditions under which the experiment was conducted, the diquat, paraquat and glufosinate ammonium herbicides can be used for pre-harvest desiccation in the seed production fields.

Development of Accelerated Aging Test Methodology and Specimen for Bonded CFRP Systems

2009 ◽  
Vol 6 (3) ◽  
pp. 101976
Author(s):  
J. Deng ◽  
J. E. Tanner ◽  
C. W. Dolan ◽  
D. Mukai ◽  
Andreas T. Wolf ◽  
...  
Keyword(s):  
Accelerated Aging ◽  
Test Methodology ◽  
Accelerated Aging Test ◽  
Aging Test

Assessment of soybean seed vigour tests for eastern Australia

1996 ◽  
Vol 36 (1) ◽  
pp. 99
Author(s):  
M Boersma ◽  
M Law ◽  
SW Adkins
Keyword(s):  
Incubation Period ◽  
Seedling Emergence ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Field Capacity ◽  
Seed Vigour ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Eastern Australia ◽  
Controlled Deterioration

Vigour testing was undertaken on seed from 4 soybean [Glycine max (L.) Merr.] cultivars taken from field trials in eastern Australia. The seed from each cultivar exhibited moderate to good germination and a range in vigour. Vigour tests consisted of the controlled deterioration and modified versions of the accelerated aging test. Accelerated aging was carried out in 4 ways using 2 periods of incubation (76 and 96 h), with or without the seed protectant Thiram to control saprophytic fungi. Results were compared with seedling emergence from the same seed samples when planted in a controlled environment glasshouse. A combination of 2 day/night temperatures, cold (18/13�C), warm (33/28�C), and 3 soil moisture regimes, dry (-0.3 MPa), field capacity (-0.01 MPa) and saturated soil, produced a total of 6 different environments. Emergence was also recorded under a moderate daylnight temperature of 23/18�C in soil at field capacity. The standard germination test and modified accelerated aging test with a 72-h incubation period with Thiram were significantly (P<0.01) correlated with emergence from all 7 soil environments. A 96-h period of accelerated aging (with or without Thiram) tended to suppress germination and there was no correlation with emergence from most soil environments. The controlled deterioration test correlated with seedling emergence from most soil environments except for dry soil. The vigour test which gave the best indicator of performance under a range of different seedbed moisture and temperature conditions was the modified accelerated aging test using a 72-h incubation period with Thiram.

scholarly journals Seed vigor of Piptadenia moniliformis Benth. subjected to thermal stress

2020 ◽  
Vol 42 ◽  
pp. e51287
Author(s):  
Joyce Naiara da Silva ◽  
Monalisa Alves Diniz da Silva
Keyword(s):  
Electrical Conductivity ◽  
Sulfuric Acid ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Saturated Solution ◽  
Aging Period ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality ◽  
Concentrated Sulfuric Acid

 The objective of this work is to evaluate the influence of accelerated aging test at 41ºC on the germination and seed vigor of Piptadenia moniliformis Benth. Two experiments were conducted. Experiment 1: seeds were submitted to dormancy overcoming treatments. They were immersed into sulfuric acid for 0 (intact seeds), 5, 10, 15, 20 and 25 minutes. Experiment 2: two methods were evaluated for the accelerated aging test at 41ºC, that is, the traditional method and the saturated NaCl solution method with exposure periods of 0, 24, 48, 72 and 96 hours. After each treatment, the seeds were subjected to electrical conductivity and germination tests. Seeds overcame their dormancy when immersed into concentrated sulfuric acid for 5, 10, 15, 20 or 25 minutes, but the germination was faster when immersed for 20 minutes. The different methods for the accelerated aging test, decreased the germination potential and seed vigor of P. moniliformis after 24 hours of exposure. Regarding the method used, the saturated solution provided, in general, the best results since the seed water content was lower, thus reducing the proliferation of fungi. The electrical conductivity test showed that, by increasing the aging period regardless of the method, there is an increase in seed deterioration, corroborating with germination results, which showed that the correlation was moderate and negative and indicated that the higher the conductivity, the lower the percentage and the germination speed index. The immersion of seeds of P. moniliformis into concentrated sulfuric acid for 20 minutes provides a fast and increased germination. The methods for accelerated aging at 41ºC, traditional and saturated solution, can be used as vigor tests to evaluate the physiological quality of seeds.

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