scholarly journals Effect of seed coat rupture on the germination of Mimosa flocculosa Burkart (Leguminosae) seeds

Hoehnea ◽  
2020 ◽  
Vol 47 ◽  
Author(s):  
Maiara Iadwizak Ribeiro ◽  
Guilherme Almeida Garcia Rodrigues ◽  
Gabriele Larissa Mathias ◽  
Shirley Martins Silva ◽  
Jaqueline Malagutti Corsato ◽  
...  
Keyword(s):  
Seed Coat ◽  
Native Species ◽  
Large Scale ◽  
Germination Percentage ◽  
Distilled Water ◽  
Seedling Production ◽  
Physical Dormancy ◽  
Anatomical Analysis ◽  
Heated Water ◽  
Mean Germination Time

ABSTRACT Mimosa flocculosa Burkart is a native species used for the recovery of degraded areas. Large scale seedling production in nurseries is hindered due to seed physical dormancy. This study evaluated the germination and seed coat anatomy of M. flocculosa seeds subjected to dormancy overcome treatments. Seeds of M. flocculosa were subjected to: control with distilled water, heated water at 50, 80 and 100 °C, and H2SO4 for 1, 3 and 5 minutes. Germination tests were conducted with four replicates of 25 seeds in an entirely casualized delineation, analyzing germination percentage, mean germination time and germination speed index. Moreover, anatomical analysis of the seed coat was conducted. The treatments with heated water at 80 ºC, and sulfuric acid for 3 and 5 minutes promoted benefic anatomical alterations in the seed integument, reflecting in higher and faster in germination rates. Heated water at 80 ºC is recommended because of its efficiency and safety.

scholarly journals Physiological, physical, and morpho-anatomical changes in Libidibia ferrea ((Mart. ex Tul.) L.P. Queiroz) seeds after overcoming dormancy

2015 ◽  
Vol 37 (1) ◽  
pp. 26-32 ◽  
Author(s):  
Antônio César Batista Matos ◽  
Glauciana da Mata Ataíde ◽  
Eduardo Euclydes de Lima e Borges
Keyword(s):  
Sulfuric Acid ◽  
Seed Coat ◽  
Germination Percentage ◽  
Seedling Production ◽  
Forest Species ◽  
Physical Dormancy ◽  
Anatomical Changes ◽  
Time Of Application ◽  
Before And After ◽  
Triphasic Pattern

Seed dormancy is a phenomenon that affects the distribution of plant species in time. However, it may slow germination and consequently hinder seedling production in nurseries. Many seeds of forest species of the Fabaceae family, such as Libidibia ferrea (Brazilian ironwood), have physical dormancy caused by impermeability of the seed coat to water. Therefore, the aim of this study was to evaluate the physiological, physical, and morpho-anatomical characteristics of L. ferrea seeds before and after application of different treatments for overcoming dormancy. We evaluated the imbibition curves, germination percentage, germination speed index (GSI), the force required to puncture the micropylar region, and morpho-anatomical changes through images obtained from a scanning electron microscope (SEM) before and after application of treatments for overcoming dormancy. L. ferrea seeds immersed in sulfuric acid show a triphasic pattern of imbibition. The required force to puncture the micropylar region of L. ferrea seeds is less for both the treatment with boiling water and for treatments with sulfuric acid. In addition, the required force to puncture the micropylar region decreases during imbibition of the seeds after application of sulfuric acid. The time of application of sulfuric acid influences the thickness of the material removed from the macrosclereid layer of the seed coat.

scholarly journals Plant growth regulators in the germination of Diospyros hispida A. DC. (EBENACEAE) seeds

2021 ◽  
Vol 10 (2) ◽  
pp. e27110212471
Author(s):  
Aline Bueno Ramalho ◽  
Lúcia Filgueiras Braga
Keyword(s):  
Plant Growth ◽  
Gibberellic Acid ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Germination Rate ◽  
Dry Mass ◽  
Shoot Length ◽  
Distilled Water ◽  
Seedling Production ◽  
Mean Germination Time

Plant growth regulators in the germination of Diospyros hispida A. DC. (Ebenaceae) Seed. Diospyros hispida species is a native fruit that has long germination period, hindering seedling production. The aim of this work was to evaluate the germination of Diospyros hispida seeds under the effect of gibberellic acid and biostimulant applications. Four replicates of 25 seeds per treatment were used. Treatments were: T1 - distilled water, T2, T3 and T4 - GA3 solutions at 100, 150 and 200 mg L-1, respectively, T5 - Immersion for 24 hours in distilled water with aeration, T6, T7 and T8 - Immersion for 24 hours in aerated GA3 solutions at 100, 150 and 200 mg L-1, respectively, T9, T10 and T11 - biostimulant application (containing auxin, gibberellin and cytokinin) at 3, 5 and 7 ml kg-1 of seeds. Germination rate, mean germination time, germination speed index, synchronization index, relative germination frequency, normal seedlings, root length, shoot length and seedling dry mass were evaluated. The effect of gibberellic acid and biostimulant containing auxin, gibberellin and cytokinin negatively influenced Diospyros hispida germination under the conditions of this study.

scholarly journals Efectividad de algunos tratamientos pre-germinativos para ocho especies leñosas de la Mixteca Alta oaxaqueña con características relevantes para la restauración

2017 ◽  
pp. 9
Author(s):  
Gilberto Martínez-Pérez ◽  
Alma Orozco-Segovia ◽  
Carlos Martorell
Keyword(s):  
Seed Coat ◽  
Native Species ◽  
Immature Embryo ◽  
New Method ◽  
Dodonaea Viscosa ◽  
Physical Dormancy ◽  
Mixteca Alta ◽  
Physiological Dormancy ◽  
Wearing Off ◽  
Do So

When restoring highly degraded areas such as the Mixteca Alta (Oaxaca State, Mexico), it is important to use native species that promote natural succession. To do so, we need to know whether their seeds have dormancy and how to break it. We compared different pre-germination treatments of eight species relevant for restoration. The results were analyzed with a new method that solves some of the statistical problems that arise when examining these experiments. In Acacia schaffneri, Ipomoea murucoides, Mimosa aculeaticarpa and Dodonaea viscosa wearing off the seed coat by means of abrasion or heating promotes rapid germination, proving the presence of physical dormancy. Despite belonging to families that show physiological dormancy only, the seeds of Arctostaphylos pungens and Juniperus flaccida germinate after immersion in acid. This procedure may have weakened the seed coat, allowing the immature embryo to break it. We found weak physiological dormancy in Quercus deserticola, and no apparent dormancy in Quercus castanea.

Chloride salts inhibit emergence and seedling growth of chickpea rather than germination

2017 ◽  
Vol 40 (04) ◽  
Author(s):  
Hamdi Özaktan ◽  
Cemalettin Yasar Çiftçi Çiftçi ◽  
Mehmet Demir KAYA ◽  
Sati Uzun ◽  
Oguzhan Uzun ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Seedling Growth ◽  
Genotypic Variation ◽  
Germination Percentage ◽  
Distilled Water ◽  
Germination Test ◽  
Seedling Length ◽  
Mean Germination Time ◽  
Chloride Salts ◽  
Chickpea Cultivars

The effects of chloride salts (NaCl, MgCl2 and CaCl2) with the same electrical conductivity (EC) values on germination, emergence, seedling growth and Cl content of seedling in chickpea cultivars (Akçin 91, Aziziye, Gökçe, Inci, Iºýk-05 and Yaºa-05) were investigated. The EC values of the chloride salts were adjusted to 4, 8 and 16 dS m-1 and the distilled water was served as control. Germination percentage, mean germination time, radicula and plumula lengths, fresh and dry radicula and plumula weight, chloride (Cl-) content of seedling in germination test, and seedling length, fresh and dry seedling weight in emergence test were measured. The results revealed that increasing salt doses caused a significant reduction in parameters of all cultivars. The dose of 16 dS m-1 from each salt was found to be lethal. Cl- content of seedling was increased from 0.05 ppm in control to 4.10 ppm in 16 dS m-1; resulting in decreasing germination, emergence and seedling growth. The cultivar Aziziye presented better performance and gave higher emergence rates in NaCl, while Iºýk-05 in CaCl2 and Gökçe in MgCl2; indicating that there was a genotypic variation towards different salt sources. It was concluded that emergence and seedling growth were severely influenced by increasing salinity without changing germination and emergence was not possible at 16 dS m-1.

Overcoming Dormancy and Influence of Light on the Physiological Quality of Senna cana (Fabaceae) Seeds

2019 ◽  
pp. 1-9
Author(s):  
Jordânia X. Medeiros ◽  
Ana L. P. Feliciano ◽  
Valderez P. Matos ◽  
Girlânio H. Silva ◽  
Yana S. Lopes ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Native Species ◽  
Red Light ◽  
Distilled Water ◽  
Light Regimes ◽  
Germination Process ◽  
Physiological Quality ◽  
Mean Germination Time

Environmental factors affect the germination process, like the presence of seed coat and the quality of light; these informations are still scarce for many native species from Brazil, especially for Senna cana, which there are no adequate standards and methodologies to be used in germination tests. The aim of this research was to recommend adequate pre-germinative treatment(s) to overcome seed dormancy, and determine the degree of influence of different light regimes in seed germination of S. cana. Two experiments were carried out: T1-evaluation of different methods of dormancy overcoming (intact seeds (control), T2-imbibition of the intact seeds for 24 hours (in distilled water), T3-scarified seeds with sandpaper n° 100 in the hilum opposite region, T4-scarified seeds with sandpaper n° 100 in the region the hilo opposite region and imbibition in water (in distilled water) for 24 hours; T5-imbibition in water at 80°C); 2-Influence of light quality on seed germination and vigor (white light, red light, far red light and absence of light). The evaluated parameters were: first germination count, percentage of germination, IVG (Germination speed index), MGT (Mean germination time). Treatments were compared by Tukey at 5% probability. The best method for overcoming seed dormancy was mechanical scarification with sandpaper nº 100. The germination of the seeds of S. cana can be classified as indifferent to the luminosity, despite germinative response be greater under white and red light.

A new seed coat water-impermeability mechanism in Chaetostoma armatum (Melastomataceae): evolutionary and biogeographical implications of physiophysical dormancy

2015 ◽  
Vol 25 (2) ◽  
pp. 194-202 ◽  
Author(s):  
Rafaella C. Ribeiro ◽  
Denise M.T. Oliveira ◽  
Fernando A.O. Silveira
Keyword(s):  
Phenolic Compounds ◽  
Seed Coat ◽  
Seed Weight ◽  
Significant Fraction ◽  
Distilled Water ◽  
Physical Dormancy ◽  
First Report ◽  
Germination Ecology ◽  
Impermeable Seed Coat ◽  
Structure Water

AbstractDetermining the phylogenetic and biogeographic distribution of physical dormancy remains a major challenge in germination ecology. Here, our goal was to describe a novel water-impermeable seed coat mechanism causing physical dormancy (PY) in the seeds of Chaetostoma armatum (Melastomataceae). Although seed coat permeability tests indicated a significant increase in seed weight after soaking in distilled water, anatomical and dye-tracking analyses showed that both water and dyes penetrated the seed coat but not the embryo, which remained in a dry state. The water and dye penetrated the lumen of the exotestal cells, which have a thin outer periclinal face and thickened secondary walls with U-shaped phenolic compounds. Because of this structure, water and dye do not penetrate the inner periclinal face of the exotestal cells, indicating PY. Puncturing the seeds increased germination more than tenfold compared to that of the control, but GA3 did not increase germination further. A significant fraction of the seeds did not germinate after puncturing, indicating that embryos are also physiologically dormant (PD). This paper constitutes the first report of the water-impermeable seed coat in the Myrtales and the first report of physiophysical (PD+PY) dormancy in a shrub from a tropical montane area.

scholarly journals Seed Germination and Initial Growth of Pepper Seedlings is Influenced by Environment Temperature and Priming Method

2020 ◽  
Vol 8 (4) ◽  
pp. 540
Author(s):  
Glenda Sallaku ◽  
Gjok Vuksani ◽  
Astrit Balliu
Keyword(s):  
Polyethylene Glycol ◽  
Germination Percentage ◽  
Early Growth ◽  
Distilled Water ◽  
Initial Growth ◽  
Environment Temperature ◽  
Germination Speed ◽  
Mean Germination Time ◽  
Peg 8000 ◽  
Germination Parameters

The effects of different environment temperatures, priming treatments and priming durations on germination parameters and early growth of pepper seedlings were investigated. Seeds were hydro primed in distilled water, or osmo primed in two different water potentials (-0.15 and -0.5 MPa) of polyethylene glycol (PEG) 8000. Both, hydro primed and osmo primed seeds were subject of two different imbibitions periods, 48 and 72 hrs. In addition to primed seeds, an equal sample of non primed seeds was included in the experiment as a control variant. Following priming, the seeds were subjected to germination tests at two different environment temperatures, respectively 18 0C and 24 0C. In general, there was no significant effect of hydro priming on germination parameters or early growth of pepper seedlings. On the contrary, significantly higher germination percentage and germination speed and significantly shorter mean germination time than the non primed seeds were obtained due to seed osmo priming by PEG. Similarly, an enhanced seedling growth, especially under suboptimum temperature conditions was evidenced in PEG primed seedlings. Apart from germination speed, the increase of PEG concentration from -0.15 to -0.5 MPa had no effect to germination parameters of pepper seeds. The extent of the imbibitions period over 48 hrs did also have no benefits on germination parameters.

scholarly journals Evaluation of several methods for breaking dormancy of bitter vetch seeds (Vicia ervilia L.)

2015 ◽  
Vol 71 (1) ◽  
pp. 57-65 ◽  
Author(s):  
Hossein Reza Rouhi ◽  
Ali Sepehri ◽  
Leila Sefidkhani ◽  
Fatemeh Karimi
Keyword(s):  
Germination Percentage ◽  
Hot Water ◽  
The Other ◽  
Cold Stratification ◽  
Distilled Water ◽  
Breaking Dormancy ◽  
Bitter Vetch ◽  
Physiological Dormancy ◽  
Mean Germination Time ◽  
Germination Parameters

Abstract This study analysed the effects of different treatments on breaking dormancy and germination of bitter vetch (Vicia ervilia L.) freshly harvested seeds for seeding immediately. Partial scarified seeds (30 seconds with sandpaper) were subjected to different treatments including: GA3 (250, 500 and 750 ppm), KNO3 (0.1, 0.2 and 0.3% w/v), cold stratification (2, 4 and 6 days), sulfuric acid (25, 50 and 75 seconds), hot water (90°C; for 2.5 and 5 minutes), hydropriming via seed soaking in distilled water (4 and 8 hours) and mechanical scarification. Among the mentioned treatments, cold stratification for 6 days had a best effect on germination related parameters final germination percentage, mean germination time and vigour indexthan the other periods. In contrast to scarification with acid and hot water, mechanical scarification improved germination parameters but this effect was lower than the cold stratification. The results suggest that bitter vetch seed has both physical and physiological dormancy.

scholarly journals Hydropriming Increases Germination of Lentil (Lens culinaris Medik.) under Water Stress

2010 ◽  
Vol 2 (2) ◽  
pp. 103-106 ◽  
Author(s):  
Sevil SAĞLAM ◽  
Sibel DAY ◽  
Gamze KAYA ◽  
Ayşegül GÜRBÜZ
Keyword(s):  
Water Stress ◽  
Seed Weight ◽  
Lens Culinaris ◽  
Germination Percentage ◽  
Shoot Length ◽  
Distilled Water ◽  
Seedling Vigor ◽  
Variable Response ◽  
Mean Germination Time ◽  
Response To Water

Fresh seeds of lentil cultivars ‘Pul 11’, ‘Sultan 1’ and ‘Meyveci 2001’ were subjected to hydropriming with an objective to improve germination and seedling vigor under water stress induced by PEG-6000 at the water potentials of 0.0 (distilled water), -0.3 and-0.6 MPa. Results revealed that germination delayed in increasing water stress with variable germination among cultivars. Root, shoot length and germination were higher but mean germination time were lower in the primed seeds. Seeds were able to germinate at all concentrations of PEG but higher germination and improved seedling growth was observed in primed seeds. Cultivars showed variable response to water stress and cv. ‘Pul 11’ with the lightest seed weight gave better performance. Whereas, cv. ‘Sultan 1’ enhanced germination percentage with hydropriming under increased water stress. It was concluded that inhibition of germination due to water stress should be overcome by using primed lentil seeds.

scholarly journals Markedly different patterns of imbibition in seeds of 48 Acacia species

2019 ◽  
Vol 29 (4) ◽  
pp. 270-282 ◽  
Author(s):  
Geoffrey E. Burrows ◽  
Rowan Alden ◽  
Wayne A. Robinson
Keyword(s):  
Process Control ◽  
Seed Coat ◽  
Germination Percentage ◽  
Rain Gauge ◽  
Hot Water ◽  
Physical Dormancy ◽  
Acacia Species ◽  
Rain Events

AbstractThe seeds of most Australian acacias have pronounced physical dormancy (PY). While fire and hot water (HW) treatments cause the lens to ‘pop’ almost instantaneously, for many Acacia species the increase in germination percentage can be gradual. If PY is broken instantly by HW treatment, why is germination often an extended process? Control and HW treatments were performed on seeds of 48 species of Acacia. Seeds were placed on a moist substrate and imbibition was assessed by frequently weighing individual seeds. In the two soft-seeded species all control seeds were fully imbibed within 6–24 h, while in hard-seeded species very few control seeds imbibed over several weeks. In 10 species over 50% of the HW-treated seeds imbibed within 30 h, but mostly the percentage of imbibed seeds gradually increased over several weeks. Some seeds in a replicate would imbibe early, while others would remain unimbibed for many days or weeks then, remarkably, become fully imbibed in less than 24 h. While HW treatment broke PY almost instantaneously, it appeared that in many Acacia species some other part of the testa slowed water from reaching the embryo. This process of having staggered imbibition may be a way of ensuring not all seeds in a population germinate after small rain events. Thus it appears the lens acts as a ‘fire gauge’ while some other part of the seed coat acts as a ‘rain gauge’.

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