scholarly journals Meteorological conditions, ozone concentration and leaf age affect gas exchange in Psidium guajava ‛Palumaʼ

Hoehnea ◽  
2017 ◽  
Vol 44 (2) ◽  
pp. 236-245 ◽  
Author(s):  
Juliana Moreno Pina ◽  
Sérgio Tadeu Meirelles ◽  
Regina Maria de Moraes

ABSTRACT This study aimed to investigate the importance of leaf age, meteorological conditions and ozone concentration (O3) on gas exchange of Psidium guajava ‛Paluma'. Saplings were grown and exposed in standard conditions in the city of São Paulo, in six periods of three months with weekly measurements in young and mature leaves. Gas exchanges were higher in young leaves for almost the entire experiment. Mature leaves showed greater reduction in gas exchange. The multivariate analysis of biotic and abiotic variables indicated that vapor pressure deficit (VPD), O3 concentration and radiation were the main variables associated with gas exchange decrease in young leaves. In mature leaves the influence of VPD is lower, but the temperature importance is higher. Moreover, the opposition between assimilation and O3 is more evident in mature leaves, indicating their greater sensitivity to O3.

2010 ◽  
Vol 40 (6) ◽  
pp. 1290-1294 ◽  
Author(s):  
Inês Cechin ◽  
Natália Corniani ◽  
Terezinha de Fátima Fumis ◽  
Ana Catarina Cataneo

The effects of water stress and rehydration on leaf gas exchange characteristics along with changes in lipid peroxidation and pirogalol peroxidase (PG-POD) were studied in mature and in young leaves of sunflower (Helianthus annuus L.), which were grown in a greenhouse. Water stress reduced photosynthesis (Pn), stomatal conductance (g s), and transpiration (E) in both young and mature leaves. However, the amplitude of the reduction was dependent on leaf age. The intercellular CO2 concentration (Ci) was increased in mature leaves but it was not altered in young leaves. Instantaneous water use efficiency (WUE) in mature stressed leaves was reduced when compared to control leaves while in young stressed leaves it was maintained to the same level as the control. After 24h of rehydration, most of the parameters related to gas exchange recovered to the same level as the unstressed plants except gs and E in mature leaves. Water stress did not activated PG-POD independently of leaf age. However, after rehydration the enzyme activity was increased in mature leaves and remained to the same as the control in young leaves. Malondialdehyde (MDA) content was increased by water stress in both mature and young leaves. The results suggest that young leaves are more susceptible to water stress in terms of gas exchange characteristics than mature leaves although both went through oxidative estresse.


2021 ◽  
Vol 12 ◽  
Author(s):  
Sylvain Pincebourde ◽  
Jérôme Ngao

Herbivore insects have strong impacts on leaf gas exchange when feeding on the plant. Leaf age also drives leaf gas exchanges but the interaction of leaf age and phloem herbivory has been largely underexplored. We investigated the amplitude and direction of herbivore impact on leaf gas exchange across a wide range of leaf age in the apple tree–apple green aphid (Aphis pomi) system. We measured the gas exchange (assimilation and transpiration rates, stomatal conductance and internal CO2 concentration) of leaves infested versus non-infested by the aphid across leaf age. For very young leaves up to 15 days-old, the gas exchange rates of infested leaves were similar to those of non-infested leaves. After few days, photosynthesis, stomatal conductance and transpiration rate increased in infested leaves up to about the age of 30 days, and gradually decreased after that age. By contrast, gas exchanges in non-infested leaves gradually decreased across leaf age such that they were always lower than in infested leaves. Aphids were observed on relatively young leaves up to 25 days and despite the positive effect on leaf photosynthesis and leaf performance, their presence negatively affected the growth rate of apple seedlings. Indeed, aphids decreased leaf dry mass, leaf surface, and leaf carbon content except in old leaves. By contrast, aphids induced an increase in leaf nitrogen content and the deviation relative to non-infested leaves increased with leaf age. Overall, the impacts of aphids at multiple levels of plant performance depend on leaf age. While aphids cause an increase in some leaf traits (gas exchanges and nitrogen content), they also depress others (plant growth rate and carbon content). The balance between those effects, as modulated by leaf age, may be the key for herbivory mitigation in plants.


Materials ◽  
2019 ◽  
Vol 12 (15) ◽  
pp. 2498 ◽  
Author(s):  
Sperdouli ◽  
Moustaka ◽  
Antonoglou ◽  
Adamakis ◽  
Dendrinou-Samara ◽  
...  

Young and mature leaves of Arabidopsis thaliana were exposed by foliar spray to 30 mg L−1 of CuZn nanoparticles (NPs). The NPs were synthesized by a microwave-assisted polyol process and characterized by dynamic light scattering (DLS), X-ray diffraction (XRD), and transmission electron microscopy (TEM). CuZn NPs effects in Arabidopsis leaves were evaluated by chlorophyll fluorescence imaging analysis that revealed spatiotemporal heterogeneity of the quantum efficiency of PSII photochemistry (ΦPSΙΙ) and the redox state of the plastoquinone (PQ) pool (qp), measured 30 min, 90 min, 180 min, and 240 min after spraying. Photosystem II (PSII) function in young leaves was observed to be negatively influenced, especially 30 min after spraying, at which point increased H2O2 generation was correlated to the lower oxidized state of the PQ pool.. Recovery of young leaves photosynthetic efficiency appeared only after 240 min of NPs spray when also the level of ROS accumulation was similar to control leaves. On the contrary, a beneficial effect on PSII function in mature leaves after 30 min of the CuZn NPs spray was observed, with increased ΦPSΙΙ, an increased electron transport rate (ETR), decreased singlet oxygen (1O2) formation, and H2O2 production at the same level of control leaves.An explanation for this differential response is suggested.


1997 ◽  
Vol 37 (1) ◽  
pp. 119 ◽  
Author(s):  
D. O. Huett ◽  
A. P. George ◽  
J. M. Slack ◽  
S. C. Morris

Summary. A leaf nutrient survey was conducted of the low-chill peach cultivars, Flordaprince (October maturing) and Flordagold (mid November–early December maturing) at 3 commercial sites in both northern New South Wales and southern Queensland. Recently mature leaves from the middle third of a current season’s fruiting lateral (spring flush) were sampled at stone hardening and 2-weeks postharvest and of a non-fruiting lateral at maturity of the summer flush (after summer pruning) during the 1992–93 and 1993–94 seasons. At an additional site in New South Wales (Alstonville), leaf nutrient concentrations were also determined on cv. Flordagem (early November maturing) at 2-week intervals during both seasons. Soil (0–30 cm) chemical determinations were conducted at all sites at 2-weeks postharvest Seasonal trends in leaf nutrient composition were associated with a leaf age–maturity effect. As flush leaves matured during spring, and as mature leaves aged after hardening of the summer flush, nitrogen (N) concentration declined and calcium (Ca) concentration increased. Nitrogen and Ca concentrations increased when young leaves produced from the summer flush were sampled. Time of sampling produced the most consistently significant (P<0.05) main effects on leaf nutrient concentration. The 2-week postharvest period was selected as a convenient time to sample—when leaves were of a consistent age and maturity, and the effect of crop load on tree nutrient reserves was still present. Paclobutrazol, which reduces vegetative growth in stonefruit, was applied to all Queensland sites and, as a consequence, mid lateral leaves contained higher (P<0.05) Ca, magnesium (Mg) and chloride (Cl) and lower (P<0.05) N and phosphorus (P) concentrations than leaves from New South Wales sites. State effects can therefore be interpreted as paclobutrazol effects. Cultivar effects (P<0.05) occurred for many leaf nutrients, however, at the 2-week postharvest sampling, concentrations were sufficiently similar to combine as a narrow adequate concentration range for both cultivars. The diagnostic adequate leaf nutrient concentrations were within the range developed for high-chill peaches (Leece et al. 1971) with the exception of lower Ca, lower Mg for New South Wales (both cultivars), lower iron for Flordaprince (both states), higher P for Flordaprince in New South Wales and higher manganese values for Queensland (both cultivars). Regression analyses were conducted between leaf and fruit nutrient concentrations and soil chemical properties. The only consistent result demonstrated that as the soil Ca : Mg ratio increased, leaf Mg concentration decreased exponentially (P<0.001), indicating that the practice of heavy annual agricultural limestone or gypsum applications in the absence of Mg fertiliser, which had been adopted by several growers in the survey, is associated with lower leaf Mg concentrations.


2006 ◽  
Vol 33 (5) ◽  
pp. 457 ◽  
Author(s):  
Amy E. Zanne ◽  
Steven S. Lower ◽  
Zoe G. Cardon ◽  
Colin M. Orians

Allocation of root-derived resources is influenced by tissue demand; however, vascular pathways mediate resource flow from roots to shoots. In vascularly constrained plants (i.e. sectored plants), effects of vascular connections likely limit homogenous resource delivery, especially when environmental resource distribution is patchy. Here, we quantify relative roles of vascular connections, demands by different leaves (i.e. by leaf age and size), and molecule size of transported N compounds (effective sectoriality: nitrate v. ammonium) on allocation of 15N in the sectored tomato (Solanum lycopersicum L.). Vascular connections were the strongest predictor of both accumulation (amount per leaf; P<0.0001) and δ (estimate of concentration; P<0.0001) 15N values in mature leaves, but young expanding leaves did not show such dramatically sectored uptake (accumulation: P=0.0685; δ: P=0.0455), suggesting that sectoriality is less strong in young expanding tissue, especially in the youngest leaf. In patchy environments sectoriality, then, should have large consequences for the ability of a plant to allocate resources in mature tissue; however, young leaves do not appear to experience such strong vascular constraints when building new tissue.


2006 ◽  
Vol 131 (3) ◽  
pp. 320-326 ◽  
Author(s):  
Woei-Jiun Guo ◽  
Nean Lee

In this study, effects of leaf age (20 to 240 days), plant age (4, 8, and 14 months after deflasking), and various day/night temperature regimes (16 to 33 °C) on photosynthesis of Phalaenopsis amabilis L. Blume var. formosa Shimadzu (Phal. TS97) leaves were investigated. The diurnal net CO2 uptake in Phal. TS97 leaves was measured and integrated to obtain total net CO2 uptake, which represents photosynthetic efficiency in plants performing crassulacean acid metabolism (CAM). Under all conditions, Phal. TS97 leaves performed typical CAM photosynthesis and reached their highest net CO2 uptake rate, ≈6 μmol·m-2·s-1, after 3 to 4 hours in the dark under a 12-hour photoperiod. When grown under 30 °C day/25 °C night temperature, the total net CO2 uptake of leaf increased with maturation and was highest at 80 days old, 20 days after full expansion. The CAM photosynthetic capacity of mature leaves remained high after maturation and began to decline at a leaf age of 240 days. The trend was consistent with malate fixation but the highest nocturnal malate concentration was observed in 100-day-old leaves. Young leaves or leaves from small juvenile plants had higher daytime CO2 fixation compared to mature leaves or large plants, suggesting that Phal. TS97 leaves progressed from C3-CAM to CAM during the course of maturation. The second newly matured leaf from the top had the highest net CO2 fixation when the newest leaf was 8 cm in length. Although plant age did not influence total CO2 uptake in the leaf, photosynthetic efficiency of leaves in small younger plants was more sensitive to high light intensity, 340 μmol·m-2·s-1 photosynthetic photon flux. The day/night temperature of 32/28 and 29/25 °C resulted in the highest total net CAM CO2 fixation in vegetative Phal. TS97 plants than higher (33/29 °C) and lower temperatures (21/16 °C).


HortScience ◽  
1995 ◽  
Vol 30 (5) ◽  
pp. 1017-1019 ◽  
Author(s):  
W.F. Whitehead ◽  
B.P. Singh

Two studies were conducted to assess the effects of leaf aging on gas exchange in okra [Abelmoschus esculentus (L.) Moench] leaves. Gas exchange was measured at 6- to 10-day intervals starting 15 days after leaf emergence (DFE) and continuing until senescence at 50 DFE. Rates of transpiration (E), stomatal conductance (gs) and CO2 exchange (CER) increased as leaves matured up to ≈25 DFE, about full leaf expansion. Transpiration rate, gs, and CER declined after 25 DFE and as leaves aged further. Internal leaf CO2 concentration (Ci) was higher in old than young leaves. This study suggests that the most efficient okra canopy would maximize exposure of 25-day-old leaves to sunlight.


Agrotek ◽  
2018 ◽  
Vol 2 (3) ◽  
Author(s):  
Antonius Suparno ◽  
Opalina Logo ◽  
Dwiana Wasgito Purnomo

Sweet potato serves as a staple food for people in Jayawijaya. Many cultivars of sweet potatoes have been cultivated by Dani tribe in Kurulu as foot for their infant, child and adult as well as feeding especially for pigs. Base on the used of sweet potatoes as food source for infant and child, this study explored 10 different cultivars. As for the leaf morphology, it was indentified that the mature leaves have size around 15 � 18 cm. general outline of the leaf is reniform (40%), 60% have green colour leaf, 50% without leaf lobe, 60% of leaf lobes number is one, 70% of shape of central leaf lobe is toothed. Abazial leaf vein pigmentation have purple (40%), and petiole pigmentation is purple with green near leaf (60%), besides its tuber roots, sweet potatoes are also harvested for its shoots and green young leaves for vegetables.


HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 501c-501
Author(s):  
Andrés A. Estrada-Luna ◽  
Jonathan N. Egilla ◽  
Fred T. Davies

The effect of mycorrhizal fungi on gas exchange of micropropagated guava plantlets (Psidium guajava L.) during acclimatization and plant establishment was determined. Guava plantlets (Psidium guajava L. cv. `Media China') were asexually propagated through tissue culture and acclimatized in a glasshouse for eighteen weeks. Half of the plantlets were inoculated with ZAC-19, which is a mixed isolate containing Glomus etunicatum and an unknown Glomus spp. Plantlets were fertilized with modified Long Ashton nutrient solution containing 11 (g P/ml. Gas exchange measurements included photosynthetic rate (A), stomatal conductance (gs), internal CO2 concentration (Ci), transpiration rate (E), water use efficiency (WUE), and vapor pressure deficit (VPD). Measurements were taken at 2, 4, 8 and 18 weeks after inoculation using a LI-6200 portable photosynthesis system (LI-COR Inc. Lincoln, Neb., USA). Two weeks after inoculation, noninoculated plantlets had greater A compared to mycorrhizal plantlets. However, 4 and 8 weeks after inoculation, mycorrhizal plantlets had greater A, gs, Ci and WUE. At the end of the experiment gas exchange was comparable between noninoculated and mycorrhizal plantlets.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Shugang Zhao ◽  
Hongxia Wang ◽  
Kai Liu ◽  
Linqing Li ◽  
Jinbing Yang ◽  
...  

Abstract Background Tissue culture is an effective method for the rapid breeding of seedlings and improving production efficiency, but explant browning is a key limiting factor of walnut tissue culture. Specifically, the polymerization of PPO-derived quinones that cause explant browning of walnut is not well understood. This study investigated explants of ‘Zanmei’ walnut shoot apices cultured in agar (A) or vermiculite (V) media, and the survival percentage, changes in phenolic content, POD and PPO activity, and JrPPO expression in explants were studied to determine the role of PPO in the browning of walnut explants. Results The results showed that the V media greatly reduced the death rate of explants, and 89.9 and 38.7% of the explants cultured in V media and A media survived, respectively. Compared with that of explants at 0 h, the PPO of explants cultured in A was highly active throughout the culture, but activity in those cultured in V remained low. The phenolic level of explants cultured in A increased significantly at 72 h but subsequently declined, and the content in the explants cultured in V increased to a high level only at 144 h. The POD in explants cultured in V showed high activity that did not cause browning. Gene expression assays showed that the expression of JrPPO1 was downregulated in explants cultured in both A and V. However, the expression of JrPPO2 was upregulated in explants cultured in A throughout the culture and upregulated in V at 144 h. JrPPO expression analyses in different tissues showed that JrPPO1 was highly expressed in stems, young leaves, mature leaves, catkins, pistils, and hulls, and JrPPO2 was highly expressed in mature leaves and pistils. Moreover, browning assays showed that both explants in A and leaf tissue exhibited high JrPPO2 activity. Conclusion The rapid increase in phenolic content caused the browning and death of explants. V media delayed the rapid accumulation of phenolic compounds in walnut explants in the short term, which significantly decreased explants mortality. The results suggest that JrPPO2 plays a key role in the oxidation of phenols in explants after branch injury.


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