Glycosaminoglycans and vitamin C in ovo feeding affects bone characteristics of chicks

Abstract Background: Some previous studies have indicated that in ovo feeding (IOF) of vitamin C (VC) had positive effects on the performance in poultry. In order to realize embryonic VC supplementation, an idea about hen’s dietary VC supplementation to achieve VC enrichment in produced eggs was proposed. And this study was executed to investigate the effects of dietary VC supplementation on synthesis and transportation of VC in layers and VC deposition status in produced eggs.Results: Compared with Arbor Acres breeder eggs, egg VC content was lower in Isa Brown breeder eggs and Hy-Line Brown layer eggs (P < 0.05). Sodium-dependent vitamin C transporter 1 (SVCT1) and SVCT2 expression was higher in ileum than in duodenum and jejunum (P < 0.05). SVCT1 expression was extremely higher in magnum than in ovary, while SVCT2 expression was lower (P < 0.05). L-gulonolactone oxidase (GLO) expression was extremely higher and SVCT1 expression was higher in kidney than in liver, while SVCT2 was lower (P < 0.05). 400 mg/kg VC supplementation increased SVCT1 expression in duodenum, ovary and magnum, while decreased GLO and SVCT1 expression in liver (P < 0.05). 200 and 400 mg/kg VC supplementation increased SVCT2 expression in duodenum, while decreased GLO and SVCT1 expression in kidney and SVCT2 expression in liver (P < 0.05).Conclusions: Hy-Line Brown layer was a useful model for investigating effects of dietary VC supplementation on VC deposition in produced eggs. Dietary VC supplementation promoted VC absorption in duodenum and jejunum, but reduced endogenous VC synthesis in liver and kidney. Although dietary VC supplementation enhanced VC transportation in ovary and magnum, it finally failed to increase VC deposition in produced eggs.

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Effect of in ovo feeding of vitamin C on antioxidation and immune function of broiler chickens

animal ◽

10.1017/s1751731118003531 ◽

2019 ◽

Vol 13 (9) ◽

pp. 1927-1933 ◽

Cited By ~ 6

Author(s):

Y.F. Zhu ◽

S.Z. Li ◽

Q.Z. Sun ◽

X.J. Yang

Keyword(s):

Vitamin C ◽

Immune Function ◽

Broiler Chickens ◽

In Ovo ◽

In Ovo Feeding

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Exploring the effectiveness of in ovo feeding of vitamin C based on the embryonic vitamin C synthesis and absorption in broiler chickens

Journal of Animal Science and Biotechnology ◽

10.1186/s40104-021-00607-w ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Yufei Zhu ◽

Jianfei Zhao ◽

Chenxu Wang ◽

Fei Zhang ◽

Xinhuo Huang ◽

...

Keyword(s):

Heat Stress ◽

Vitamin C ◽

Injection Site ◽

Broiler Chickens ◽

Yolk Sac ◽

In Ovo ◽

Sodium Dependent ◽

Gulonolactone Oxidase ◽

In Ovo Feeding ◽

Selection Of

Abstract Background Many researches about in ovo feeding (IOF) of vitamin C (VC) are gradually carried out to explore physiological development in chicken, but little studies focus on VC synthesis capacity of the embryo itself, the selection of injection site and the effectiveness of IOF of VC. This study aims to explore the above problems. Results Kidney and yolk sac were the main organs for VC synthesis and L-gulonolactone oxidase (GLO) expression was lower during pre-hatch development than that during post-hatch development. Sodium-dependent vitamin C transporter 1 (SVCT1) expression was increased continuously in yolk sac from embryonic age 19 (E19) to post-hatch day 1 (D1) and in intestine (duodenum, jejunum and ileum) from E17 to D1. Plasma VC content was higher at D1 than that at D21 and D42. IOF of VC significantly reduced GLO expression in liver, kidney and yolk sac as well as SVCT1 expression in duodenum, jejunum and ileum, but increased the VC content in plasma, brain, kidney and liver. In addition, IOF of VC obviously reduced the embryonic morality and increased the hatchability under heat stress. Conclusions This study suggested that IOF of VC at E11 in yolk was effective for embryonic VC supplementation. These findings provide a theoretical reference about the method of embryonic VC supplementation and effective methodology on embryonic VC nutrition in broiler chickens.

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Effects of in ovo feeding of vitamin E or vitamin C on egg hatchability, performance, carcass traits and immunity in broiler chickens

Animal Biotechnology ◽

10.1080/10495398.2021.1950744 ◽

2021 ◽

pp. 1-6

Author(s):

Farhad Ghane ◽

Ali-Ahmad-Alaw Qotbi ◽

Marina Slozhenkina ◽

Aleksander Anatolievich Mosolov ◽

Ivan Gorlov ◽

...

Keyword(s):

Vitamin E ◽

Vitamin C ◽

Broiler Chickens ◽

Carcass Traits ◽

In Ovo ◽

Egg Hatchability ◽

In Ovo Feeding

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Effects of low eggshell temperatures during incubation, in ovo feeding of L-arginine, and posthatch dietary guanidinoacetic acid on hatching traits, performance, and physiological responses of broilers reared at low ambient temperature

Poultry Science ◽

10.1016/j.psj.2021.101548 ◽

2021 ◽

pp. 101548

Author(s):

Bahram Miri ◽

Hossein Ali Ghasemi ◽

Iman Hajkhodadadi ◽

Amir Hossein Khaltabadi Farahani

Keyword(s):

Ambient Temperature ◽

Physiological Responses ◽

In Ovo ◽

Guanidinoacetic Acid ◽

In Ovo Feeding ◽

Low Ambient Temperature

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İn ovo Vitamin C ve E Enjeksiyonunun Isı Stresine Maruz Kalan Etlik Piliçlerde Gelişme Performansı ve Oksidatif Stabilite Üzerine Etkileri

Ege Üniversitesi Ziraat Fakültesi Dergisi ◽

10.20289/zfdergi.387798 ◽

2017 ◽

pp. 259-266

Author(s):

Özge Altan ◽

Zümrüt Açıkgöz ◽

Özer Hakan Bayraktar ◽

Fadime Aydın Köse ◽

Çiğdem Şeremet Tuğalay ◽

...

Keyword(s):

Vitamin C ◽

In Ovo

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Effect of in ovo feeding of L-glutamine + ISP to chick embryos

Medicina Veterinária (UFRPE) ◽

10.26605/medvet-v13n4-3669 ◽

2019 ◽

Vol 13 (4) ◽

pp. 591

Author(s):

João Paulo Ferreira Rufino ◽

Frank George Guimarães Cruz ◽

Valcely Da Rocha Costa ◽

André Ferreira Silva ◽

Pedro Alves de Oliveira Filho ◽

...

Keyword(s):

Chick Embryos ◽

In Ovo ◽

In Ovo Feeding

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Effect of in ovo feeding of dextrin-iodinated casein in broilers: II. Hatch window and growth performance

Poultry Science ◽

10.3382/ps/pew439 ◽

2017 ◽

Vol 96 (5) ◽

pp. 1478-1484 ◽

Cited By ~ 2

Author(s):

S. Abousaad ◽

K. Lassiter ◽

A. Piekarski ◽

P. Chary ◽

K. Striplin ◽

...

Keyword(s):

Growth Performance ◽

In Ovo ◽

In Ovo Feeding

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Effects of in ovo feeding of vitamin C on post-hatch performance, immune status and DNA methylation-related gene expression in broiler chickens

British Journal Of Nutrition ◽

10.1017/s000711452000210x ◽

2020 ◽

Vol 124 (9) ◽

pp. 903-911 ◽

Cited By ~ 1

Author(s):

Yufei Zhu ◽

Shizhao Li ◽

Yulan Duan ◽

Zhouzheng Ren ◽

Xin Yang ◽

...

Keyword(s):

Gene Expression ◽

Dna Methylation ◽

Vitamin C ◽

Antioxidant Capacity ◽

Broiler Chickens ◽

Immune Status ◽

Stimulation Index ◽

Average Daily Gain ◽

Related Gene ◽

In Ovo

AbstractThis study aimed to evaluate the effect of in ovo feeding (IOF) of vitamin C at embryonic age 11 (E11) on post-hatch performance, immune status and DNA methylation-related gene expression in broiler chickens. A total of 240 Arbor Acres breeder eggs (63 (sem 0·5) g) were randomly divided into two groups: normal saline and vitamin C (VC) groups. After incubation, newly hatched chicks from each group were randomly divided into six replicates with ten chicks per replicate. Hatchability, average daily feed intake (D21–42 and D1–42), and average daily gain and feed conversion ratio (D1–21) were improved by vitamin C treatment (P < 0·05). IOF of vitamin C increased vitamin C content (D1), total antioxidant capacity (D42), IgA (D1), IgM (D1 and D21), stimulation index for T lymphocyte (D35) and lysozyme activity (D21) in plasma (P < 0·05). On D21, vitamin C increased the splenic expression of IL-4 and DNMT1 and decreased IL-1β, Tet2, Tet3 and Gadd45β expression (P < 0·05). On D42, vitamin C increased the splenic expression of IL-4 and DNMT3A and decreased IFN-γ, Tet3, MBD4 and TDG expression (P < 0·05). In conclusion, the vitamin C via in ovo injection can be absorbed by broiler’s embryo and IOF of vitamin C at E11 improves the post-hatch performance and immune status and, to some extent, the antioxidant capacity of broiler chickens. The expression of enzyme-related DNA methylation and demethylation indicates that the level of DNA methylation may increase in spleen in the VC group and whether the fluctuating expression of pro- and anti-inflammatory cytokines is related to DNA methylation change remained to be further investigated.

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