scholarly journals Exploring the effectiveness of in ovo feeding of vitamin C based on the embryonic vitamin C synthesis and absorption in broiler chickens

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yufei Zhu ◽  
Jianfei Zhao ◽  
Chenxu Wang ◽  
Fei Zhang ◽  
Xinhuo Huang ◽  
...  
Keyword(s):  
Heat Stress ◽  
Vitamin C ◽  
Injection Site ◽  
Broiler Chickens ◽  
Yolk Sac ◽  
In Ovo ◽  
Sodium Dependent ◽  
Gulonolactone Oxidase ◽  
In Ovo Feeding ◽  
Selection Of

Abstract Background Many researches about in ovo feeding (IOF) of vitamin C (VC) are gradually carried out to explore physiological development in chicken, but little studies focus on VC synthesis capacity of the embryo itself, the selection of injection site and the effectiveness of IOF of VC. This study aims to explore the above problems. Results Kidney and yolk sac were the main organs for VC synthesis and L-gulonolactone oxidase (GLO) expression was lower during pre-hatch development than that during post-hatch development. Sodium-dependent vitamin C transporter 1 (SVCT1) expression was increased continuously in yolk sac from embryonic age 19 (E19) to post-hatch day 1 (D1) and in intestine (duodenum, jejunum and ileum) from E17 to D1. Plasma VC content was higher at D1 than that at D21 and D42. IOF of VC significantly reduced GLO expression in liver, kidney and yolk sac as well as SVCT1 expression in duodenum, jejunum and ileum, but increased the VC content in plasma, brain, kidney and liver. In addition, IOF of VC obviously reduced the embryonic morality and increased the hatchability under heat stress. Conclusions This study suggested that IOF of VC at E11 in yolk was effective for embryonic VC supplementation. These findings provide a theoretical reference about the method of embryonic VC supplementation and effective methodology on embryonic VC nutrition in broiler chickens.

scholarly journals Dynamic Alterations on Synthesis and Transportation of Vitamin C and Deposition Status in Produced Eggs Induced by Dietary Vitamin C Supplementation in Hy-Line Brown Layer Model

2020 ◽  
Author(s):  
Yufei Zhu ◽  
Jianfei Zhao ◽  
Wei Guo ◽  
Kailong Qin ◽  
Jiakun Yan ◽  
...  
Keyword(s):  
Vitamin C ◽  
Dietary Vitamin ◽  
Layer Model ◽  
In Ovo ◽  
Positive Effects ◽  
Sodium Dependent ◽  
Gulonolactone Oxidase ◽  
Vitamin C Supplementation ◽  
Liver And Kidney ◽  
In Ovo Feeding

Abstract Background: Some previous studies have indicated that in ovo feeding (IOF) of vitamin C (VC) had positive effects on the performance in poultry. In order to realize embryonic VC supplementation, an idea about hen’s dietary VC supplementation to achieve VC enrichment in produced eggs was proposed. And this study was executed to investigate the effects of dietary VC supplementation on synthesis and transportation of VC in layers and VC deposition status in produced eggs.Results: Compared with Arbor Acres breeder eggs, egg VC content was lower in Isa Brown breeder eggs and Hy-Line Brown layer eggs (P < 0.05). Sodium-dependent vitamin C transporter 1 (SVCT1) and SVCT2 expression was higher in ileum than in duodenum and jejunum (P < 0.05). SVCT1 expression was extremely higher in magnum than in ovary, while SVCT2 expression was lower (P < 0.05). L-gulonolactone oxidase (GLO) expression was extremely higher and SVCT1 expression was higher in kidney than in liver, while SVCT2 was lower (P < 0.05). 400 mg/kg VC supplementation increased SVCT1 expression in duodenum, ovary and magnum, while decreased GLO and SVCT1 expression in liver (P < 0.05). 200 and 400 mg/kg VC supplementation increased SVCT2 expression in duodenum, while decreased GLO and SVCT1 expression in kidney and SVCT2 expression in liver (P < 0.05).Conclusions: Hy-Line Brown layer was a useful model for investigating effects of dietary VC supplementation on VC deposition in produced eggs. Dietary VC supplementation promoted VC absorption in duodenum and jejunum, but reduced endogenous VC synthesis in liver and kidney. Although dietary VC supplementation enhanced VC transportation in ovary and magnum, it finally failed to increase VC deposition in produced eggs.

scholarly journals Effect of in ovo feeding of vitamin C on antioxidation and immune function of broiler chickens

animal ◽  
2019 ◽  
Vol 13 (9) ◽  
pp. 1927-1933 ◽  
Author(s):  
Y.F. Zhu ◽  
S.Z. Li ◽  
Q.Z. Sun ◽  
X.J. Yang
Keyword(s):  
Vitamin C ◽  
Immune Function ◽  
Broiler Chickens ◽  
In Ovo ◽  
In Ovo Feeding

Effects of in ovo feeding of vitamin E or vitamin C on egg hatchability, performance, carcass traits and immunity in broiler chickens

2021 ◽  
pp. 1-6
Author(s):  
Farhad Ghane ◽  
Ali-Ahmad-Alaw Qotbi ◽  
Marina Slozhenkina ◽  
Aleksander Anatolievich Mosolov ◽  
Ivan Gorlov ◽  
...  
Keyword(s):  
Vitamin E ◽  
Vitamin C ◽  
Broiler Chickens ◽  
Carcass Traits ◽  
In Ovo ◽  
Egg Hatchability ◽  
In Ovo Feeding

scholarly journals Splenic Gene Expression Signatures in Slow-Growing Chickens Stimulated in Ovo with Galactooligosaccharides and Challenged with Heat

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 474 ◽  
Author(s):  
Elzbieta Pietrzak ◽  
Aleksandra Dunislawska ◽  
Maria Siwek ◽  
Marco Zampiga ◽  
Federico Sirri ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heat Stress ◽  
Broiler Chickens ◽  
Physiological Saline ◽  
Negative Effects ◽  
In Ovo ◽  
Stress Related Genes ◽  
The Impact ◽  
Slow Growing ◽  
Resistance To Heat

Galactooligosaccharides (GOS) that are delivered in ovo improve intestinal microbiota composition and mitigate the negative effects of heat stress in broiler chickens. Hubbard hybrids are slow-growing chickens with a high resistance to heat. In this paper, we determined the impact of GOS delivered in ovo on slow-growing chickens that are challenged with heat. The experiment was a 2 × 2 × 2 factorial design. On day 12 of incubation, GOS (3.5 mg/egg) was delivered into the egg (n = 300). Controls (C) were mock-injected with physiological saline (n = 300). After hatching, the GOS and C groups were split into thermal groups: thermoneutral (TN) and heat stress (HS). HS (30 °C) lasted for 14 days (days 36–50 post-hatching). The spleen (n = 8) was sampled after acute (8.5 h) and chronic (14 days) HS. The gene expression of immune-related (IL-2, IL-4, IL-6, IL-10, IL-12p40, and IL-17) and stress-related genes (HSP25, HSP90AA1, BAG3, CAT, and SOD) was detected with RT-qPCR. Chronic HS up-regulated the expression of the genes: IL-10, IL-12p40, SOD (p < 0.05), and CAT (p < 0.01). GOS delivered in ovo down-regulated IL-4 (acute p < 0.001; chronic p < 0.01), IL-12p40, CAT and SOD (chronic p < 0.05). The obtained results suggest that slow-growing hybrids are resistant to acute heat and tolerant to chronic heat, which can be supported with in ovo GOS administration.

Effects of in ovo feeding of vitamin C on post-hatch performance, immune status and DNA methylation-related gene expression in broiler chickens

2020 ◽  
Vol 124 (9) ◽  
pp. 903-911 ◽  
Author(s):  
Yufei Zhu ◽  
Shizhao Li ◽  
Yulan Duan ◽  
Zhouzheng Ren ◽  
Xin Yang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Vitamin C ◽  
Antioxidant Capacity ◽  
Broiler Chickens ◽  
Immune Status ◽  
Stimulation Index ◽  
Average Daily Gain ◽  
Related Gene ◽  
In Ovo

AbstractThis study aimed to evaluate the effect of in ovo feeding (IOF) of vitamin C at embryonic age 11 (E11) on post-hatch performance, immune status and DNA methylation-related gene expression in broiler chickens. A total of 240 Arbor Acres breeder eggs (63 (sem 0·5) g) were randomly divided into two groups: normal saline and vitamin C (VC) groups. After incubation, newly hatched chicks from each group were randomly divided into six replicates with ten chicks per replicate. Hatchability, average daily feed intake (D21–42 and D1–42), and average daily gain and feed conversion ratio (D1–21) were improved by vitamin C treatment (P < 0·05). IOF of vitamin C increased vitamin C content (D1), total antioxidant capacity (D42), IgA (D1), IgM (D1 and D21), stimulation index for T lymphocyte (D35) and lysozyme activity (D21) in plasma (P < 0·05). On D21, vitamin C increased the splenic expression of IL-4 and DNMT1 and decreased IL-1β, Tet2, Tet3 and Gadd45β expression (P < 0·05). On D42, vitamin C increased the splenic expression of IL-4 and DNMT3A and decreased IFN-γ, Tet3, MBD4 and TDG expression (P < 0·05). In conclusion, the vitamin C via in ovo injection can be absorbed by broiler’s embryo and IOF of vitamin C at E11 improves the post-hatch performance and immune status and, to some extent, the antioxidant capacity of broiler chickens. The expression of enzyme-related DNA methylation and demethylation indicates that the level of DNA methylation may increase in spleen in the VC group and whether the fluctuating expression of pro- and anti-inflammatory cytokines is related to DNA methylation change remained to be further investigated.

scholarly journals Effects of in ovo feeding of N-acetyl-L-glutamate on early intestinal development and growth performance in broiler chickens

2020 ◽  
Vol 99 (7) ◽  
pp. 3583-3593
Author(s):  
Jiguang Wang ◽  
Jing Lin ◽  
Jing Wang ◽  
Shugeng Wu ◽  
Guanghai Qi ◽  
...  
Keyword(s):  
Growth Performance ◽  
Broiler Chickens ◽  
Intestinal Development ◽  
In Ovo ◽  
In Ovo Feeding

Post hatch recovery of a probiotic Enterococcus faecium strain in the yolk sac and intestinal tract of broiler chickens after in ovo injection

2019 ◽  
Vol 366 (7) ◽  
Author(s):  
Line Skjøt-Rasmussen ◽  
Dorthe Sandvang ◽  
Alfred Blanch ◽  
Jette Mundus Nielsen ◽  
Tina Styrishave ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Broiler Chickens ◽  
Intestinal Tract ◽  
Yolk Sac ◽  
In Ovo ◽  
In Ovo Injection ◽  
Faecium Strain

Effect of induction of thermotolerance with vitamin C, E supplementation on performance broiler chickens reared at during heat stress

2005 ◽  
Vol 2005 ◽  
pp. 169-169
Author(s):  
S. Roshani ◽  
A. M. Tahmasbi ◽  
A. Taghizadeh ◽  
M. Valizadeh
Keyword(s):  
Heat Stress ◽  
Vitamin C ◽  
Feed Efficiency ◽  
Broiler Chickens ◽  
Environmental Temperature ◽  
Live Weight ◽  
Early Age ◽  
Nutrient Supplementation ◽  
Carcass Characteristic ◽  
Negative Effect

The stress of high environmental temperature may have a deleterious influence on the performance of broiler chickens by reducing feed intake, live weight gain and feed efficiency. Exposing chicks to 36-38°C for 24h at 5d of age reinforces the resistance of older (6 to 7 wk-old) broilers to heat stress (De Basillo et al., 2003). Several studies have revealed that antioxidant nutrient supplementation especially vitamin C and E can be used to alter the negative effect of environmental stress (NRC, 1984). Combination of antioxidant vitamins generally shows greater antioxidant activity than that of each component alone. The objective of this study was to investigate the effects of early age thermal conditioning and vitamin C, E supplementation on performance, carcass characteristic in broiler chickens reared under heat stress.

scholarly journals Post hatch recovery of a probiotic Enterococcus faecium strain in the yolk sac and intestinal tract of broiler chickens after in ovo injection

2019 ◽  
Vol 366 (Supplement_1) ◽  
pp. i92-i96
Author(s):  
Line Skjøt-Rasmussen ◽  
Dorthe Sandvang ◽  
Alfred Blanch ◽  
Jette Mundus Nielsen ◽  
Tina Styrishave ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Broiler Chickens ◽  
Intestinal Tract ◽  
Yolk Sac ◽  
Resistant Bacteria ◽  
In Ovo ◽  
Animal Products ◽  
Immune Health ◽  
Environmental Pathogens ◽  
Hatching Eggs

ABSTRACT Concerns about antibiotic-resistant bacteria and their presence in animal products grow and thus alternatives to use of antibiotics in animal production are being investigated. Probiotics have gained increased focus due to improvements in performance, immune health and pathogen reduction when provided to poultry through feed. These traits may be further improved if probiotics can be provided to the embryo before hatch, before meeting environmental pathogens. The objective was to determine the faith of a probiotic Enterococcus faecium (M74) strain in the yolk sac and intestinal tract of broiler chickens after injection into hatching eggs. E. faecium M74 (1.4 × 107 CFU/egg) was applied in ovo at day 18 of incubation. From 1- and 7-day-old chickens, 20 samples from yolk sac, caecal tonsils and rest of the intestinal tract were subjected to CFU counting. Isolates from a sample subset were typed by pulsed-field gel electrophoresis (PFGE). Enterococci were found in varying numbers: 1.0 × 104–2.2 × 1010 CFU/g. The prevalence of M74 PFGE profiles was high in 1-day-old (88%) and 7-day-old chickens (67%). This demonstrates that the embryos ingested M74 before hatching, that M74 is viable for intestinal colonization through in ovo administration, and that the strain multiplies in the chickens gastrointestinal tract post hatching.

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