Effect of in ovo feeding of vitamin C on antioxidation and immune function of broiler chickens

Abstract Background Many researches about in ovo feeding (IOF) of vitamin C (VC) are gradually carried out to explore physiological development in chicken, but little studies focus on VC synthesis capacity of the embryo itself, the selection of injection site and the effectiveness of IOF of VC. This study aims to explore the above problems. Results Kidney and yolk sac were the main organs for VC synthesis and L-gulonolactone oxidase (GLO) expression was lower during pre-hatch development than that during post-hatch development. Sodium-dependent vitamin C transporter 1 (SVCT1) expression was increased continuously in yolk sac from embryonic age 19 (E19) to post-hatch day 1 (D1) and in intestine (duodenum, jejunum and ileum) from E17 to D1. Plasma VC content was higher at D1 than that at D21 and D42. IOF of VC significantly reduced GLO expression in liver, kidney and yolk sac as well as SVCT1 expression in duodenum, jejunum and ileum, but increased the VC content in plasma, brain, kidney and liver. In addition, IOF of VC obviously reduced the embryonic morality and increased the hatchability under heat stress. Conclusions This study suggested that IOF of VC at E11 in yolk was effective for embryonic VC supplementation. These findings provide a theoretical reference about the method of embryonic VC supplementation and effective methodology on embryonic VC nutrition in broiler chickens.

Download Full-text

Effects of in ovo feeding of vitamin E or vitamin C on egg hatchability, performance, carcass traits and immunity in broiler chickens

Animal Biotechnology ◽

10.1080/10495398.2021.1950744 ◽

2021 ◽

pp. 1-6

Author(s):

Farhad Ghane ◽

Ali-Ahmad-Alaw Qotbi ◽

Marina Slozhenkina ◽

Aleksander Anatolievich Mosolov ◽

Ivan Gorlov ◽

...

Keyword(s):

Vitamin E ◽

Vitamin C ◽

Broiler Chickens ◽

Carcass Traits ◽

In Ovo ◽

Egg Hatchability ◽

In Ovo Feeding

Download Full-text

Effects of in ovo feeding of vitamin C on post-hatch performance, immune status and DNA methylation-related gene expression in broiler chickens

British Journal Of Nutrition ◽

10.1017/s000711452000210x ◽

2020 ◽

Vol 124 (9) ◽

pp. 903-911 ◽

Cited By ~ 1

Author(s):

Yufei Zhu ◽

Shizhao Li ◽

Yulan Duan ◽

Zhouzheng Ren ◽

Xin Yang ◽

...

Keyword(s):

Gene Expression ◽

Dna Methylation ◽

Vitamin C ◽

Antioxidant Capacity ◽

Broiler Chickens ◽

Immune Status ◽

Stimulation Index ◽

Average Daily Gain ◽

Related Gene ◽

In Ovo

AbstractThis study aimed to evaluate the effect of in ovo feeding (IOF) of vitamin C at embryonic age 11 (E11) on post-hatch performance, immune status and DNA methylation-related gene expression in broiler chickens. A total of 240 Arbor Acres breeder eggs (63 (sem 0·5) g) were randomly divided into two groups: normal saline and vitamin C (VC) groups. After incubation, newly hatched chicks from each group were randomly divided into six replicates with ten chicks per replicate. Hatchability, average daily feed intake (D21–42 and D1–42), and average daily gain and feed conversion ratio (D1–21) were improved by vitamin C treatment (P < 0·05). IOF of vitamin C increased vitamin C content (D1), total antioxidant capacity (D42), IgA (D1), IgM (D1 and D21), stimulation index for T lymphocyte (D35) and lysozyme activity (D21) in plasma (P < 0·05). On D21, vitamin C increased the splenic expression of IL-4 and DNMT1 and decreased IL-1β, Tet2, Tet3 and Gadd45β expression (P < 0·05). On D42, vitamin C increased the splenic expression of IL-4 and DNMT3A and decreased IFN-γ, Tet3, MBD4 and TDG expression (P < 0·05). In conclusion, the vitamin C via in ovo injection can be absorbed by broiler’s embryo and IOF of vitamin C at E11 improves the post-hatch performance and immune status and, to some extent, the antioxidant capacity of broiler chickens. The expression of enzyme-related DNA methylation and demethylation indicates that the level of DNA methylation may increase in spleen in the VC group and whether the fluctuating expression of pro- and anti-inflammatory cytokines is related to DNA methylation change remained to be further investigated.

Download Full-text

Effects of in ovo feeding of N-acetyl-L-glutamate on early intestinal development and growth performance in broiler chickens

Poultry Science ◽

10.1016/j.psj.2020.04.003 ◽

2020 ◽

Vol 99 (7) ◽

pp. 3583-3593

Author(s):

Jiguang Wang ◽

Jing Lin ◽

Jing Wang ◽

Shugeng Wu ◽

Guanghai Qi ◽

...

Keyword(s):

Growth Performance ◽

Broiler Chickens ◽

Intestinal Development ◽

In Ovo ◽

In Ovo Feeding

Download Full-text

The effects of in ovo feeding of glutamine in broiler breeder eggs on hatchability, development of the gastrointestinal tract, growth performance and carcass characteristics of broiler chickens

Archives Animal Breeding ◽

10.5194/aab-59-235-2016 ◽

2016 ◽

Vol 59 (2) ◽

pp. 235-242 ◽

Cited By ~ 6

Author(s):

Mehdi Salmanzadeh ◽

Yahya Ebrahimnezhad ◽

Habib Aghdam Shahryar ◽

Jamshid Ghiasi Ghaleh-Kandi

Keyword(s):

Gastrointestinal Tract ◽

Growth Performance ◽

Broiler Chickens ◽

Carcass Characteristics ◽

Abdominal Fat ◽

Control Group ◽

Villus Height ◽

In Ovo ◽

Crypt Depth ◽

In Ovo Feeding

Abstract. The aim of the present study was to investigate the effect of in ovo feeding (IOF) of glutamine on hatchability, development of the gastrointestinal tract, growth performance and carcass characteristics of broiler chickens. Fertilized eggs were subjected to injections with glutamine (Gln) (10, 20, 30, 40 or 50 mg dissolved in 0.5 mL of dionized water) on day 7 of incubation. Hatchability, growth performance, carcass characteristics (carcass weight and relative weights of breast, thigh, heart, liver, gizzard, abdominal fat, intestine, pancreas and spleen) and jejunal morphometry (measurement of villus height and width and crypt depth) were determined during the experiment. The weight of newly hatched chickens was significantly greater in groups with Gln injection than in control and sham groups. But IOF caused lower hatchability than in the control group (non-injected eggs) (p < 0.05). Chickens from IOF of Gln showed better weight gain and feed conversion ratio (0–42 days of age), when compared to chickens hatched from control and sham groups. The IOF of Gln significantly increased villus height, villus width and crypt depth at hatch period and villus height at 42 days of age. In addition, carcass weights and relative weights of breast, thigh and gizzard were also markedly increased in chickens treated in ovo with Gln; whereas heart, liver, abdominal fat, intestine, pancreas and spleen were not significantly altered at the end of the experimental period. These data suggest that the IOF of Gln may improve jejunum development, leading to an increased nutrient assimilation and consequently to greater performance in broiler chickens.

Download Full-text

Dynamic Alterations on Synthesis and Transportation of Vitamin C and Deposition Status in Produced Eggs Induced by Dietary Vitamin C Supplementation in Hy-Line Brown Layer Model

10.21203/rs.3.rs-38944/v1 ◽

2020 ◽

Author(s):

Yufei Zhu ◽

Jianfei Zhao ◽

Wei Guo ◽

Kailong Qin ◽

Jiakun Yan ◽

...

Keyword(s):

Vitamin C ◽

Dietary Vitamin ◽

Layer Model ◽

In Ovo ◽

Positive Effects ◽

Sodium Dependent ◽

Gulonolactone Oxidase ◽

Vitamin C Supplementation ◽

Liver And Kidney ◽

In Ovo Feeding

Abstract Background: Some previous studies have indicated that in ovo feeding (IOF) of vitamin C (VC) had positive effects on the performance in poultry. In order to realize embryonic VC supplementation, an idea about hen’s dietary VC supplementation to achieve VC enrichment in produced eggs was proposed. And this study was executed to investigate the effects of dietary VC supplementation on synthesis and transportation of VC in layers and VC deposition status in produced eggs.Results: Compared with Arbor Acres breeder eggs, egg VC content was lower in Isa Brown breeder eggs and Hy-Line Brown layer eggs (P < 0.05). Sodium-dependent vitamin C transporter 1 (SVCT1) and SVCT2 expression was higher in ileum than in duodenum and jejunum (P < 0.05). SVCT1 expression was extremely higher in magnum than in ovary, while SVCT2 expression was lower (P < 0.05). L-gulonolactone oxidase (GLO) expression was extremely higher and SVCT1 expression was higher in kidney than in liver, while SVCT2 was lower (P < 0.05). 400 mg/kg VC supplementation increased SVCT1 expression in duodenum, ovary and magnum, while decreased GLO and SVCT1 expression in liver (P < 0.05). 200 and 400 mg/kg VC supplementation increased SVCT2 expression in duodenum, while decreased GLO and SVCT1 expression in kidney and SVCT2 expression in liver (P < 0.05).Conclusions: Hy-Line Brown layer was a useful model for investigating effects of dietary VC supplementation on VC deposition in produced eggs. Dietary VC supplementation promoted VC absorption in duodenum and jejunum, but reduced endogenous VC synthesis in liver and kidney. Although dietary VC supplementation enhanced VC transportation in ovary and magnum, it finally failed to increase VC deposition in produced eggs.

Download Full-text

Performance, intestinal morphometry, and incubation parameters of broiler chickens submitted to in ovo feeding with different techniques and amino acids

Canadian Journal of Animal Science ◽

10.1139/cjas-2018-0131 ◽

2019 ◽

Vol 99 (4) ◽

pp. 732-740

Author(s):

Priscila Groff-Urayama ◽

Joselaine Padilha ◽

Suelen Einsfeld ◽

Simone Pertile ◽

Mateus Gorges ◽

...

Keyword(s):

Amino Acids ◽

Broiler Chickens ◽

Relative Weight ◽

Control Group ◽

In Ovo ◽

Air Chamber ◽

Significant Difference ◽

Hatching Eggs ◽

Intestinal Morphometry ◽

In Ovo Feeding

The objective of this research was to evaluate techniques, incubation period, and effects of amino acids in ovo feeding. First, 240 hatching eggs were selected and distributed in a 2 × 3 factorial arrangement (two techniques and 3 d). The parameters of incubation, relative weight of gastrointestinal organs, and classification of embryonic mortality were evaluated. In the second stage, 720 hatching eggs were incubated with five treatments: control, methionine (20 and 30 mg), and lysine (20 and 30 mg). These animals were housed during 14 d. The animals were divided with five treatments, nine replicates, and groupings based on sex. Incubation parameters, performance, intestinal morphometry, and relative weight of digestive tract organs were evaluated. The results indicate that the technique using a 45° needle axis passing through the air chamber hinders hatchability. In the organ weighing, there was no difference between the evaluated factors. For intestinal morphometry, there was only a significant difference between the dose factor for the crypt villi and crypt diameter. To conclude, the technique using a 90° angle without passing through the air chamber is safer for the embryos. The inoculation of methionine (20 and 30 mg) obtained data similar to the control group.

Download Full-text

The effects of in ovo feeding of threonine and carbohydrates on growth performance of broiler chickens

Proceedings of the British Society of Animal Science ◽

10.1017/s1752756200030374 ◽

2009 ◽

Vol 2009 ◽

pp. 198-198

Author(s):

S N Mousavi ◽

F Foroudi ◽

F Arab Baghi ◽

M Shivazad ◽

H Ghahri

Keyword(s):

Gastrointestinal Tract ◽

Growth Performance ◽

Broiler Chickens ◽

Muscle Development ◽

High Energy ◽

Broiler Chicks ◽

In Ovo ◽

Before And After ◽

Efficient Uptake ◽

In Ovo Feeding

The few days before and after hatch is a critical period for the development and survival of commercial broilers. Glycogen reserves in the chick embryo are significantly depleted during the perihatch period in order to meet the high energy demands during the process of emergence (Uni and Ferket, 2004). The immediate posthatch period is characterized by a transition from the use of lipid-rich yolk as the nutrient source to exogenous feed rich in carbohydrates and proteins. This transition is accompanied by rapid physical and functional development of the gastrointestinal tract (Sklan, 2001). The accelerated rate of development posthatch is reflected in the several-fold improvement in the development of the gastrointestinal tract and organ growth which will allow for a more efficient uptake of nutrients for muscle development. During late embryogenesis, solutions administered into the amniontic fluid (in ovo feeding) are consumed by the embryo, digested, and absorbed by the embryonic intestine prior to pipping (Uni et al., 2005). In ovo feeding of supplemental nutrients may help to overcome the constraint of limited egg nutrients (Foye et al., 2006). The objective of this experiment was to evaluate the effects of in ovo feeding of carbohydrates and threonine, alone or in combination, on growth performance of broiler chicks from 1 to 42 d of age.

Download Full-text