scholarly journals Evidence of active herpesvirus 6 (variant-A) infection in patients with lymphadenopathy in Belém, Pará, Brazil

2003 ◽  
Vol 45 (5) ◽  
pp. 283-288 ◽  
Author(s):  
Ronaldo B. Freitas ◽  
Maria R. Freitas ◽  
Alexandre C. Linhares
Keyword(s):  
Nested Pcr ◽  
Age Groups ◽  
Human Herpesvirus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Active Infection ◽  
Serum Samples ◽  
Recent Infection ◽  
Cervical Lymph Nodes ◽  
Duration Of Symptoms ◽  
Herpesvirus 6

A total of 323 patients with lymphadenopathy were selected in Belém, Brazil, between January 1996 and December 2001, and screened for the presence of human herpesvirus-6 (HHV-6) IgM- and- IgG antibodies by enzyme-linked immunosorbent assay (ELISA). When seroprevalence is analyzed by gender, similar rates are found for female (60.6%) and male (55.7%) individuals. Seventy-seven (23.8%) patients were HHV-6-IgM-and- IgG-positive (IgM+ subgroup), with positivity rates of 29.7% and 17.7% (p = 0.0007) for female- and male individuals, respectively. Sera from a subgroup (n = 120) of these subjects, with high HHV-6 antibody levels (either IgM+ or IgG+ reactivities), were subsequently processed for the presence of HHV-6 DNA by polymerase chain reaction (PCR)/nested PCR. Active infections (IgM+ and/or IgG+ high levels specific antibodies plus detection of viral DNA) were diagnosed in 20/77 (20.0%) and 8/43 (18.6%); subgroup of the 120 individuals suspected of having HHV-6 suggestive recent infection. All (n = 28) cases of active infection were found to be associated with HHV-6 variant-A (HHV-6A), as detectable by PCR/nested PCR, using variant-specific primer that amplify regions of 195 base pairs (bp) (HHV-6A) and 423 bp (HHV-6B). Rates of HHV-6 DNA detection between female and male patients were similar (p > 0.05) in the IgM+ and IgG+ groups: 20.4% versus 35.7% and 25.0% versus 13.0%, respectively. HHV-6 DNA was detected across < 5 through 41-50-year age-groups for patients whose serum samples were IgM+, with rates ranging from 7.7% (female subjects aged < 5 years) to 80.0% (male, 11-20 years). Among patients whose serological status was IgG+, HHV-6 DNA was detected in < 5, 6-10, 21-30 and > 50 age-groups at rates that ranged from 15.4% (male, < 5 years of age) to 100.0% (female aged 11-20 years). Swelling cervical lymph nodes were the most common sign, accounting for 9 (32.0%) cases in each gender group. Among patients (n = 28) with active infection by HHV-6A variant, duration of symptoms lasted 1-5 days in 35.7% of subjects, whereas in 64.3% of them the disease lasted 6-20 days. Our data suggest that it is worth seeking for HHV-6 infection whenever a patient (infant or adult) presents with lymphadenopathy as a prominent symptom in the course of an acute febrile illness.

scholarly journals Prevalence of human herpesvirus 8 antibodies in the population of Belém, Pará, Brazil

2002 ◽  
Vol 44 (6) ◽  
pp. 309-313 ◽  
Author(s):  
Ronaldo B. FREITAS ◽  
Maria Rute FREITAS ◽  
Alexandre C. LINHARES
Keyword(s):  
Geometric Mean ◽  
Age Groups ◽  
Human Herpesvirus ◽  
Human Herpesvirus 8 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Herpesvirus 8 ◽  
North Brazil ◽  
Significant Difference ◽  
Male Subjects

Serum samples from 497 children and adults inhabiting two neighbourhoods (Guamá and Terra Firme) in Belém, Pará, North Brazil were screened for the presence of human herpesvirus 8 (HHV-8) antibody using an enzyme-linked immunosorbent assay. An overall 16.3% prevalence was found for these urban communities. Taken both genders together, prevalence rates of HHV-8 antibody increase gradually, across age-groups, ranging from 12.0% to 33.3%. When seroprevalence is analysed by gender, similar rates are found for female (18.4%) and male (14.0%) individuals. In the former gender group, seroprevalence rates increased from 10.3%, in children <FONT FACE=Symbol>£</FONT> 10 years of age, to 30.0% in adults 41-50 years of age. Conversely, among male subjects, the prevalence of HHV-8 antibodies decreased from 13.3% in children/young adults aged <FONT FACE=Symbol>£</FONT> 10 to 20 years of age to 6.1% in adults aged 21-30 years. From the 31-40 year-old group male onwards, seropositivity rates increased gradually, ranging from 8.3% to 66.7%. A significant difference in seropositivity rates was noted when comparing 21-30 age groups for female and male subjects: 23.3% and 6.1%, respectively (P = 0.03). Geometric mean optical densities were found to increase slightly from the lower to the higher age-groups. Our data suggest that transmission of HHV-8 occurs frequently in the general urban population of Belém, and that prevalence of antibody seems to increase with age.

scholarly journals Specificity and Prevalence of Natural Bovine Antimannan Antibodies

1999 ◽  
Vol 6 (6) ◽  
pp. 946-952 ◽  
Author(s):  
Abhay Srinivasan ◽  
Yawei Ni ◽  
Ian Tizard
Keyword(s):  
Age Groups ◽  
Inhibitory Effect ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dependent Manner ◽  
Serum Samples ◽  
Adult Cattle ◽  
Calcium Dependent ◽  
High Concentrations ◽  
Carbohydrate Components ◽  
A Minor

ABSTRACT Immune responses to the carbohydrate components of microorganisms, mediated both by antibodies and by lectins, are an important part of host defense. In the present experiments, the specificity and presence of natural bovine antibodies against mannan, a common fungal antigen, were examined by enzyme-linked immunosorbent assay (ELISA), usingSaccharomyces cerevisiae mannan as an antigen. The results showed that all serum samples from animals of three age groups (newborn, calf, and adult) tested contained antimannan antibodies, and the titer of these antibodies increased significantly in adults. However, titers among individual adult cattle differed widely. Inhibition assays showed that yeast mannan was the strongest inhibitor.d-Mannose exhibited only a minor inhibitory effect at high concentrations. This suggests that most of these antibodies recognize an oligosaccharide-based epitope(s) different from those recognized by lectins. Cattle possess three serum C-type lectins (collectins) capable of recognizing mannan in a calcium-dependent manner. Addition of EDTA to the reaction did not reduce antibody binding, suggesting that the binding of these antibodies to mannan was not affected by the presence of collectin. The antibodies purified from either calf or adult serum by mannan-Sepharose affinity chromatography consisted of mainly immunoglobulin G (IgG) and a smaller amount of IgM. IgG1 was shown to be the dominant antimannan IgG isotype by isotype-specific ELISA. Together, these results demonstrate the production of natural antimannan antibodies in cattle in an age-dependent manner. These antibodies might be involved in defending the host against mannan-containing pathogens as a specific line of defense in conjunction with the innate response by lectins.

scholarly journals Detection of avian reovirus antibodies in layer birds of small scale commercial farms in Dinajpur district of Bangladesh

2016 ◽  
Vol 1 (3) ◽  
pp. 612-621 ◽  
Author(s):  
Abdus Salam ◽  
Md Atiqul Haque ◽  
Md Mostafizer Rahman ◽  
Mir Rowshan Akter ◽  
Farzana Afroz
Keyword(s):  
Antibody Titer ◽  
Specific Antibody ◽  
Age Groups ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Small Scale ◽  
Avian Reovirus ◽  
Serum Samples ◽  
Aged Group ◽  
Test Kit

The present study was conducted on layer birds of different age groups to determine specific antibody titer level against avian reovirus (ARV) by indirect enzyme linked immunosorbent assay (iELISA) at Dinajpur district of Bangladesh. This study showed that ARV specific antibody positive cases were 84 out of 90 blood serum samples and the highest antibody titer was 26120 and lowest antibody titer was 288. The total 93.33% sera samples were showed positive result. The study showed that 100% sera sample were positive against ARV at 6 weeks of aged group and the highest, lowest and mean antibody titer were 13917, 4895 and 10269 respectively. On the other hand 88.88% sera sample were positive against ARV at 10 weeks of aged group and the highest, lowest and mean antibody titer were 9779, 288 and 5689.89 respectively. The sera sample collected from 14 weeks of aged group showed 88.88% positive and the highest, lowest and mean antibody titer were 11727, 871 and 5250 respectively. The sera sample collected from 18 weeks of aged group showed 88.88% positive against ARV and the highest, lowest and mean antibody titer were 24440, 1234 and 12648.89 respectively. The sera sample collected from 22 weeks of aged group were 100% positive against ARV and the highest, lowest and mean antibody titer were 26120, 1752 and 11373.89 respectively. The sera sample collected from 26 weeks of aged group showed 100% positive against ARV and the highest, lowest and mean antibody titer were 8566, 1630 and 4327.44 respectively. The sera sample collected from 30 weeks of aged group showed 100% positive against ARV and the highest, lowest and mean antibody titer were 13431, 1989 and 5890.56 respectively. The sera sample collected from 40 weeks of aged group showed 77.77% positive against ARV and the highest, lowest and mean antibody titer were 14618, 433 and 5103.22 respectively. The sera sample collected from 48 weeks of aged group showed 88.88% positive against ARV and the highest, lowest and mean antibody titer were 14553, 957 and 7436.5 respectively. In conclusion it is evident that avian reovirus-specific antibody was successfully detected through commercially available avian reovirus antibody test kit (ELISA kit) and the virus induced a significant antibody titer indicating the affecting virus was absolutely ARV.Asian J. Med. Biol. Res. December 2015, 1(3): 612-621

scholarly journals Human herpes virus 8 antibodies in HIV-positive patients in Surabaya, Indonesia

2020 ◽  
Author(s):  
Devi Oktafiani ◽  
Ni Luh Ayu Megasari ◽  
Elsa Fitriana ◽  
Nasronudin ◽  
Maria Inge Lusida ◽  
...  
Keyword(s):  
Drug Users ◽  
Sandwich Elisa ◽  
Human Herpesvirus ◽  
Intravenous Drug ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hiv Positive ◽  
Intravenous Drug Users ◽  
Serum Samples ◽  
Serological Tests ◽  
Human Herpes Virus 8

Background: Human herpesvirus 8 (HHV-8) infection is etiologically related to Kaposi’s sarcoma. Antibodies directed against HHV-8 can be detected in 80%–95% of HIV-seropositive patients with KS. HHV-8 serological tests have been done in several countries in Southeast Asia such as Malaysia, and Thailand however no serological data is available in Indonesia. This study was to examine the presence of HHV-8 antibodies in HIV-positive patients in Surabaya, Indonesia. Material and methods: Ninety-one serum samples were collected from HIV-positive patients in Surabaya, Indonesia. Human immunodeficiency virus-positive serum samples were collected from 10 homosexual men, 25 intravenous drug users (IVDUs) and 56 heterosexuals. Serums were then tested for the presence of HHV-8 antibody by using sandwich ELISA (Abbexa Ltd, Cambridge, UK). Results: The total of 91 HIV-infected were testing with antibodies to HHV-8 using enzyme-linked immunosorbent assay. Antibodies of HHV-8 were detected in 7/91 (7.7%) of the samples. According to a gender, six men (85.7%) and a women (14.3%) were positive of HHV-8 antibodies. No correlation regarding the gender and age from this study. The antibodies of HHV-8 was detected among intravenous drug users (IVDUs) men 5/7 (42.8%) and 2/7 (28.6%) from homosexual and heterosexual, respectively. Conclusion: This study found the presence of HHV-8 antibodies in 7.7% of patients in Surabaya, Indonesia. This finding was higher more than Southeast Asian countries. The patients with a positive result could suggest measures to prevent HHV-8 infection.

scholarly journals Detection of human herpesvirus-7 by qualitative nested-PCR: comparison between healthy individuals and liver transplant recipients

2008 ◽  
Vol 41 (6) ◽  
pp. 556-559 ◽  
Author(s):  
Ronaldo Luis Thomasini ◽  
Juliana de Moraes Martins ◽  
Daniela Corte Parola ◽  
Sandra Helena Alves Bonon ◽  
Ilka de Fátima Santana Ferreira Boin ◽  
...  
Keyword(s):  
Liver Transplant ◽  
Healthy Volunteers ◽  
Nested Pcr ◽  
Human Herpesvirus ◽  
Transplant Recipients ◽  
Healthy Individuals ◽  
Active Infection ◽  
Serological Tests ◽  
Transplant Patients ◽  
Liver Transplant Recipients

Diagnosis of human herpesvirus-7 active infection in transplant patients has proved difficult, because this virus is ubiquitous and can cause persistent infections in the host. The significance of viral DNA detected in leukocytes by PCR is unclear and cross-reaction in serological tests may occur. This study aimed to evaluate nested-PCR to detect human herpesvirus-7 active infection in liver transplant recipients compared to healthy individuals. human herpesvirus-7 nested-PCR was performed on leukocytes and sera of 53 healthy volunteers and sera of 29 liver transplant recipients. In healthy volunteers, human herpesvirus-7 was detected in 28.3% of leukocytes and 0% of serum. human herpesvirus-7 was detected in sera of 48.2% of the liver transplant recipients. Nested-PCR on DNA extracted from leukocytes detected latent infection and the study suggests that nested-PCR performed on serum could be useful to detect human herpesvirus-7 active infection in liver transplant recipients.

scholarly journals Seroprevalence of Antibodies against Human Herpesvirus 6 in the Quebec City Area

1992 ◽  
Vol 3 (4) ◽  
pp. 179-184 ◽  
Author(s):  
Louise Deschênes ◽  
Jean R Joly ◽  
Michel Couillard ◽  
Gilles Richer
Keyword(s):  
Cell Line ◽  
Antibody Titre ◽  
Geometric Mean ◽  
Human Herpesvirus ◽  
Quebec City ◽  
Age Group ◽  
Human Herpesvirus 6 ◽  
Serum Samples ◽  
City Area ◽  
Herpesvirus 6

Seroprevalence of antibodies against human herpesvirus 6 was determined in a sample of 303 randomly selected individuals from the Quebec City area. The influence of different variables on antibody litres was also evaluated. Human herpesvirus 6 was grown in the HSB-2 cell line, and antibody litres were measured by indirect immunofluorescence. Serum samples were collected from 177 females and 126 males ranging in age from two months to 88 years. Ninety-nine per cent (300 of 303) of this population had an antibody titre of at least 1:10, whereas 75% had a titre of at least 1:80. Women had a higher geometric mean litre than men (P=0.06). This difference between sexes varied according to age and became statistically significant in subjects older than 20 years of age (P=0.04). It was found that this difference was attributable to higher antibody litres in women in the 15 to 40 year age group who had previously had children.

scholarly journals Relapsing-Remitting Multiple Sclerosis and Human Herpesvirus 6 Active Infection

2004 ◽  
Vol 61 (10) ◽  
pp. 1523 ◽  
Author(s):  
Roberto Álvarez-Lafuente ◽  
Virginia De las Heras ◽  
Manuel Bartolomé ◽  
Juan José Picazo ◽  
Rafael Arroyo
Keyword(s):  
Multiple Sclerosis ◽  
Human Herpesvirus ◽  
Human Herpesvirus 6 ◽  
Active Infection ◽  
Relapsing Remitting ◽  
Remitting Multiple Sclerosis ◽  
Herpesvirus 6 ◽  
Relapsing Remitting Multiple Sclerosis

scholarly journals Immunoglobulin G, A and M Light Chain Ratio in Children

1992 ◽  
Vol 29 (3) ◽  
pp. 271-274 ◽  
Author(s):  
Á Haraldsson ◽  
C M R Weemaes ◽  
M J H Kock-Jansen ◽  
P B J M v Eck-Arts ◽  
T de Boo ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Light Chain ◽  
Immunoglobulin G ◽  
Age Groups ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Λ Light Chain

Values for the κ/λ light chain ratio in immunoglobulins G, A and M and the total κ/λ ratio, measured by enzyme linked immunosorbent assay, were evaluated in serum samples from different age groups (114 children, aged from 1 month to 15 years, and 20 adults). The IgG κ/λ ratio decreased in the first 6 months and subsequently increased slowly during childhood towards the adult value of 2·0. The IgM κ/λ ratio increased at a greater rate than IgG κ/λ ratio in the first years of life and thereafter rose slightly throughout childhood to reach an adult value of 1·7. A decreasing IgA κ/λ ratio was found from 1 month of age onwards to an adult value of 1·1. The pattern of total κ/λ ratio was similar to the IgG κ/λ ratio with an adult value of 2·0.

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