scholarly journals Genetic diversity of dengue virus serotypes 1 and 2 in the State of Paraná, Brazil, based on a fragment of the capsid/premembrane junction region

2012 ◽  
Vol 45 (3) ◽  
pp. 297-300 ◽  
Author(s):  
Ana Caroline Dalla Bona ◽  
Adriana Lacerda Twerdochlib ◽  
Mário Antônio Navarro-Silva
Keyword(s):  
Dengue Virus ◽  
Genetic Variants ◽  
The State ◽  
Neighbor Joining ◽  
Rt Pcr ◽  
Junction Region ◽  
Serotype 1 ◽  
Dengue Virus Serotypes ◽  
Polymerase Chain ◽  
Cell Culture Line

INTRODUCTION: The precise identification of the genetic variants of the dengue virus is important to understand its dispersion and virulence patterns and to identify the strains responsible for epidemic outbreaks. This study investigated the genetic variants of the capsid-premembrane junction region fragment in the dengue virus serotypes 1 and 2 (DENV1-2). METHODS: Samples from 11 municipalities in the State of Paraná, Brazil, were provided by the Central Laboratory of Paraná. They were isolated from the cell culture line C6/36 (Aedes albopictus) and were positive for indirect immunofluorescence. Ribonucleic acid (RNA) extracted from these samples was submitted to the reverse transcription polymerase chain reaction (RT-PCR) and nested PCR. RESULTS: RT-PCR revealed that 4 of the samples were co-infected with both serotypes. The isolated DENV-1 sequences were 95-100% similar to the sequences of other serotype 1 strains deposited in GenBank. Similarly, the isolated DENV-2 sequences were 98-100% similar to other serotype 2 sequences in GenBank. According to our neighbor-joining tree, all strains obtained in this study belonged to genotype V of DENV-1. The DENV-2 strains, by contrast, belonged to the American/Asian genotypes. CONCLUSIONS: The monitoring of circulating strains is an important tool to detect the migration of virus subtypes involved in dengue epidemics.

scholarly journals IDENTIFICATION OF DENGUE VIRUS SEROTYPES AT THE DR. SOETOMO HOSPITAL SURABAYA IN 2016 AND ITS CORRELATION WITH NS1 ANTIGEN DETECTION

2018 ◽  
Vol 23 (2) ◽  
pp. 151
Author(s):  
Jeine Stela Akualing ◽  
Aryati Aryati ◽  
Puspa Wardhani ◽  
Usman Hadi
Keyword(s):  
Polymerase Chain Reaction ◽  
Dengue Virus ◽  
Reverse Transcriptase ◽  
Ribonucleic Acid ◽  
Rna Virus ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Ns1 Antigen ◽  
Dengue Virus Serotypes ◽  
Polymerase Chain

Serotipe virus dengue yang beredar terus mengalami perubahan dan berbeda di setiap daerah. Pergeseran serotipe maupun genotipedi dalamnya, mempengaruhi terjadinya wabah dengue di berbagai negara. Perbedaan serotipe diduga bernasab dengan deteksi antigen(Ag) non-structural 1 (NS1), namun belum banyak penelitian yang mendukung hal tersebut. Penelitian potong lintang dikerjakan sejakFebruari-Agustus 2016 dan didapatkan 60 subjek infeksi virus dengue (IVD) dan 25 non-IVD. Ribonucleic acid (RNA) virus denguediperiksa di semua subjek menggunakan Simplexa Dengue Real-Time Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)termasuk identifikasi serotipe virus dengue dan pemeriksaan NS1 menggunakan uji cepat NS1 Panbio. Perbedaan perbandingan variabelkategorikal dianalisis dengan uji Fisher Exact. Kenasaban antara serotipe dengan deteksi Ag NS1 dianalisis dengan Chi-Kuadrat. RNAvirus dengue terdeteksi di 43 dari 60 subjek IVD (71,7%). Serotipe terbanyak adalah DENV-3 (62,8%). Pergeseran dominasi serotipetelah terjadi di Surabaya, sebelumnya dari DENV-2 ke DENV-1 dan sekarang DENV-3, kemungkinan akibat mobilitas pejamu, transporvirus dan faktor geografis. Kepekaan uji cepat NS1 75% dan kekhasan 100%. Persentase deteksi NS1 antar serotipe berbeda bermakna(p=0,002). Deteksi NS1 lebih rendah pada DENV-1 dibandingkan DENV-2 (p=0,007) ataupun DENV-3 (p=0,003). Serotipe virusdengue bernasab dengan deteksi NS1 (p=0,005). Ciri serotipe maupun genotipe virus dengue kemungkinan mempengaruhi sekresiNS1. Telah terjadi pergeseran serotipe virus dengue di pasien IVD di Surabaya sehingga diperlukan surveillance berkesinambunganuntuk memperkirakan terjadinya wabah. Serotipe bernasab dengan deteksi NS1. Salah satu penyebab hasil negatif palsu NS1 adalahperbedaan serotipe.

scholarly journals Simultaneous circulation of all four dengue serotypes in Manaus, State of Amazonas, Brazil in 2011

2012 ◽  
Vol 45 (3) ◽  
pp. 393-394 ◽  
Author(s):  
Michele de Souza Bastos ◽  
Regina Maria Pinto de Figueiredo ◽  
Rajendranath Ramasawmy ◽  
Evaulino Itapirema ◽  
João Bosco Lima Gimaque ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Rain Forest ◽  
Capital City ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Amazon Rain Forest ◽  
Dengue Outbreaks ◽  
Dengue Virus Serotypes ◽  
Polymerase Chain ◽  
First Time

INTRODUCTION: Manaus, the capital city of the state of Amazon with nearly 2 million inhabitants, is located in the middle of the Amazon rain forest and has suffered dengue outbreaks since 1998. METHODS: In this study, blood samples were investigated using reverse transcriptase-polymerase chain reaction (RT-PCR), aimed at identifying dengue virus serotypes. RESULTS: Acute phase sera from 432 patients were tested for the presence of dengue virus. Out of the 432 patients, 137 (31.3%) were found to be positive. All the four dengue virus serotypes were observed. CONCLUSIONS: The simultaneous circulation of the four dengue serotypes is described for the first time in Manaus and in Brazil.

scholarly journals Use of immunodot blot and multiplex reverse transcriptase-polymerase chain reaction in dengue virus detection in macerates of Aedes aegypti larvae

2012 ◽  
Vol 3 (1) ◽  
pp. 13
Author(s):  
Aline T.A. Chagas ◽  
Michelle D. Oliveira ◽  
Jose M.S. Mezencio ◽  
Eduardo A.M. Silva ◽  
Leandro L. Oliveira ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Reverse Transcriptase ◽  
Epidemiological Surveillance ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Denv Serotypes ◽  
Polymerase Chain ◽  
Pcr Techniques

The <em>Dengue virus</em> is the main arbovirus that affects man in terms of morbidity and mortality. The detection of the virus is very important for epidemiological surveillance, so here we propose to standardize and compare the immunodot blot (IDB) and multiplex reverse transcriptase-polymerase chain reaction (M-RT-PCR) techniques to detect and characterize the dengue virus (DENV) serotypes in samples of <em>Aedes aegypti</em> larvae. Thus, the IDB and M-RT-PCR techniques were standardized using macerated samples of larvae collected in nature. The use of monoclonal antibodies in IDB has not shown great results, but DENV detection through this method was possible using polyclonal antibodies. The distinction of serotypes 1, 2 and 3 was carried out by M-RT-PCR.

Detection of dengue virus serotypes by single-tube multiplex RT-PCR and multiplex real-time PCR assay

2021 ◽  
Author(s):  
Kundan Tandel ◽  
Mahadevan Kumar ◽  
G.S. Bhalla ◽  
S.P.S. Shergill ◽  
Vijaya Swarnim ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Real Time ◽  
Real Time Pcr ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Single Tube ◽  
Dengue Virus Serotypes

scholarly journals Cocirculation of two dengue virus serotypes in individual and pooled samples of Aedes aegypti and Aedes albopictus larvae

2011 ◽  
Vol 44 (1) ◽  
pp. 103-105 ◽  
Author(s):  
José Eduardo Marques Pessanha ◽  
Waleska Teixeira Caiaffa ◽  
Alzira Batista Cecilio ◽  
Felipe Campos de Melo Iani ◽  
Simone Costa Araujo ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Clinical Presentation ◽  
Rt Pcr ◽  
Virus Rna ◽  
Dengue Virus Serotypes ◽  
Belo Horizonte ◽  
The City ◽  
Pooled Samples ◽  
Virological Surveillance

INTRODUCTION: To detect dengue virus, eggs of Aedes sp were collected in the city of Belo Horizonte, Brazil, in 2007. METHODS: Egg samples were subsequently hatched and the larvae were tested for the presence of dengue virus RNA by RT-PCR. RESULTS: Among the Aedes aegypti larvae samples, 163 (37.4%) out of 435 were positive, including 32 (10.9%) of 293 individual larvae samples concomitantly positive for two serotypes. CONCLUSIONS: Virological surveillance detecting coinfected vectors in the field could represent an important strategy for understanding the numerous factors involved in the transmission and clinical presentation of dengue.

Detection of concurrent infection with multiple dengue virus serotypes in Thai children by ELISA and nested RT-PCR assay

2008 ◽  
Vol 153 (12) ◽  
Author(s):  
P. Chinnawirotpisan ◽  
M. P. Mammen ◽  
A. Nisalak ◽  
B. Thaisomboonsuk ◽  
S. Narupiti ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Concurrent Infection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Dengue Virus Serotypes

scholarly journals Tracking the polyclonal neutralizing antibody response to a dengue virus serotype 1 type-specific epitope across two populations in Asia and the Americas

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Daniela V. Andrade ◽  
Colin Warnes ◽  
Ellen Young ◽  
Leah C. Katzelnick ◽  
Angel Balmaseda ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Neutralizing Antibodies ◽  
Neutralizing Antibody ◽  
Sri Lankan ◽  
Human Monoclonal Antibodies ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Dengue Virus Serotypes ◽  
Kinetics Of ◽  
Two Populations

Abstract The four dengue virus serotypes (DENV1-4) cause major public health problems worldwide. Highly neutralizing type-specific human monoclonal antibodies (hmAbs) target conformation-dependent epitopes on the DENV envelope protein, including 1F4, a DENV1 type-specific hmAb. Using a recombinant DENV2 virus displaying the DENV1 1F4 epitope (rDENV2/1), we measured the proportion and kinetics of DENV1 neutralizing antibodies targeting the 1F4 epitope in individuals living in Asia and the Americas where different DENV1 genotypes were circulating. Samples from 20 individuals were analyzed 3 and 18 months post-primary DENV1 infection, alongside samples from 4 individuals collected annually for four years post-primary DENV1 infection, from two studies in Nicaragua. We also analyzed convalescent post-primary DENV1 plasma samples from Sri Lankan individuals. We found that neutralizing antibodies recognizing the 1F4 epitope vary in prevalence across both populations and were detected from 20 days to four years post-infection. Additionally, both populations displayed substantial variability, with a range of high to low proportions of DENV1 type-specific neutralizing antibodies recognizing the 1F4 epitope seen across individuals. Thus, the 1F4 epitope is a major but not exclusive target of type-specific neutralizing antibodies post-primary infection with different DENV1 genotypes in Asia and Latin America, and additional epitopes likely contribute to type-specific neutralization of DENV1.

Prevalence of All Four Dengue Virus Serotypes Confirmed By Using Real Time RT-PCR among Population of Lahore Pakistan

2009 ◽  
Vol 3 (1) ◽  
pp. 1 ◽  
Author(s):  
Fouzia Javed ◽  
Tahir Javed ◽  
Noshin Yusuf ◽  
Abdul Mannan ◽  
Javed Akram ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Real Time ◽  
Rt Pcr ◽  
Dengue Virus Serotypes

scholarly journals System Analysis of Dengue Virus Surveillance in BBTKL PP Surabaya Year 2012–2014

2015 ◽  
Vol 3 (2) ◽  
pp. 146
Author(s):  
Atina Husnayain ◽  
Kurnia Dwi Artanti ◽  
Acub Zaenal
Keyword(s):  
Dengue Virus ◽  
Data Storage ◽  
System Analysis ◽  
Alternative Solution ◽  
Problem Analysis ◽  
Chain Reaction ◽  
Virus Surveillance ◽  
Dengue Virus Serotypes ◽  
Polymerase Chain

ABSTRACTChanging the distribution of dengue virus serotypes has occurred in Indonesia. This condition should be monitored continuously through the Dengue Virus Surveillance. Implementation of Dengue Virus Surveillance also conducted by BBTKL PP Surabaya. The purpose of this study was to determine the workflow, identify problems, set priority problem, find the cause of the problem, and provide the alternative solution related to problems of Dengue Virus Surveillance in BBTKL PP Surabaya. This is a operational research and the informants are officers of Dengue Virus Surveillance in BBTKL PP Surabaya. Data in this study was analyzed descriptive and presented narrative. Results showed that the workflow of Dengue Virus Surveillance in BBTKL PP Surabaya are collecting of patient’s blood and vector specimen, vector survey and collecting the supporting data, Rapid Diagnostic Test (RDT) and Polymerase Chain Reaction (PCR), processing and data analysis, and dissemination the information. The main problems of Dengue Virus Surveillance in BBTKL PP Surabaya is the low quality of the information. Tree problem analysis showed that the cause of problem that can be intervene are incomplete supporting data and data storage. Alternative solution related to problems of Dengue Virus Surveillance in BBTKL PP Surabaya is use of Epi Info.Keyword: surveillance, dengue virus, data management

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