scholarly journals Potential of laticifer fluids for inhibiting Aedes aegypti larval development: evidence for the involvement of proteolytic activity

2009 ◽  
Vol 104 (6) ◽  
pp. 805-812 ◽  
Author(s):  
Márcio V Ramos ◽  
Danielle A Pereira ◽  
Diego P Souza ◽  
Eliane S Araújo ◽  
Cléverson DT Freitas ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Larval Development ◽  
Proteolytic Activity

scholarly journals Production of germ-free mosquitoes via transient colonisation allows stage-specific investigation of host–microbiota interactions

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Ottavia Romoli ◽  
Johan Claes Schönbeck ◽  
Siegfried Hapfelmeier ◽  
Mathilde Gendrin
Keyword(s):  
Energy Storage ◽  
Aedes Aegypti ◽  
Larval Development ◽  
Emerging Adults ◽  
Transcriptome Analysis ◽  
Bacterial Colonisation ◽  
Germ Free ◽  
Functional Studies ◽  
Folate Biosynthesis ◽  
Vector Borne

AbstractThe mosquito microbiota impacts the physiology of its host and is essential for normal larval development, thereby influencing transmission of vector-borne pathogens. Germ-free mosquitoes generated with current methods show larval stunting and developmental deficits. Therefore, functional studies of the mosquito microbiota have so far mostly been limited to antibiotic treatments of emerging adults. In this study, we introduce a method to produce germ-free Aedes aegypti mosquitoes. It is based on reversible colonisation with bacteria genetically modified to allow complete decolonisation at any developmental stage. We show that, unlike germ-free mosquitoes previously produced using sterile diets, reversibly colonised mosquitoes show no developmental retardation and reach the same size as control adults. This allows us to uncouple the study of the microbiota in larvae and adults. In adults, we detect no impact of bacterial colonisation on mosquito fecundity or longevity. In larvae, data from our transcriptome analysis and diet supplementation experiments following decolonisation suggest that bacteria support larval development by contributing to folate biosynthesis and by enhancing energy storage. Our study establishes a tool to study the microbiota in insects and deepens our knowledge on the metabolic contribution of bacteria to mosquito development.

Effects of proteinase inhibitor from Adenanthera pavonina seeds on short- and long term larval development of Aedes aegypti

Biochimie ◽  
2015 ◽  
Vol 112 ◽  
pp. 172-186 ◽  
Author(s):  
Daniele Yumi Sasaki ◽  
Ana Cristina Jacobowski ◽  
Antônio Pancrácio de Souza ◽  
Marlon Henrique Cardoso ◽  
Octávio Luiz Franco ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Larval Development ◽  
Proteinase Inhibitor ◽  
Adenanthera Pavonina ◽  
Short And Long Term

scholarly journals Larval Development of Aedes aegypti and Aedes albopictus in Peri-Urban Brackish Water and Its Implications for Transmission of Arboviral Diseases

2011 ◽  
Vol 5 (11) ◽  
pp. e1369 ◽  
Author(s):  
Ranjan Ramasamy ◽  
Sinnathamby N. Surendran ◽  
Pavilupillai J. Jude ◽  
Sangaralingam Dharshini ◽  
Muthuladchumy Vinobaba
Keyword(s):  
Aedes Aegypti ◽  
Larval Development ◽  
Aedes Albopictus ◽  
Brackish Water ◽  
Arboviral Diseases

scholarly journals Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) Delays Egg Hatching and Larval Development of Progeny

2019 ◽  
Vol 56 (6) ◽  
pp. 1739-1744 ◽  
Author(s):  
Barbara Aparecida Chaves ◽  
Ademir Bentes Vieira Junior ◽  
Karine Renata Dias Silveira ◽  
Andreia da Costa Paz ◽  
Evelyn Beatriz da Costa Vaz ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Larval Development ◽  
Vertical Transmission ◽  
Zika Virus ◽  
Quantitative Polymerase Chain Reaction ◽  
Fourth Generation ◽  
Egg Hatching ◽  
Immature Development ◽  
F1 Progeny ◽  
Virus Exposure

Abstract Zika virus (ZIKV) has emerged as a globally important arbovirus and has been reported from all states of Brazil. The virus is primarily transmitted to humans through the bite of an infective Aedes aegypti (Linnaeus, 1762) or Aedes albopictus (Skuse, 1895). However, it is important to know if ZIKV transmission also occurs from Ae. aegypti through infected eggs to her offspring. Therefore, a ZIKV and dengue virus (DENV) free colony was established from eggs collected in Manaus and maintained until the third–fourth generation in order to conduct ZIKV vertical transmission (VT) experiments which used an infectious bloodmeal as the route of virus exposure. The eggs from ZIKV-infected females were allowed to hatch. The resulting F1 progeny (larvae, pupae, and adults) were quantitative polymerase chain reaction (qPCR) assayed for ZIKV. The viability of ZIKV vertically transmitted to F1 progeny was evaluated by cultivation in C6/36 cells. The effects of ZIKV on immature development of Ae. aegypti was assessed and compared with noninfected mosquitoes. AmazonianAe. aegypti were highly susceptible to ZIKV infection (96.7%), and viable virus passed to their progeny via VT. Moreover, eggs from the ZIKV-infected mosquitoes had a significantly lower hatch rate and the slowest hatching. In addition, the larval development period was slower when compared to noninfected, control mosquitoes. This is the first study to illustrate VT initiated by oral infection of the parental population by using mosquitoes, which originated from the field and a ZIKV strain that is naturally circulating in-country. Additionally, this study suggests that ZIKV present in the Ae. aegypti can modify the mosquito life cycle. The data reported here suggest that VT of ZIKV to progeny from naturally infected females may have a critical epidemiological role in the dissemination and maintenance of the virus circulating in the vector.

scholarly journals Knockout of juvenile hormone receptor, Methoprene-tolerant, induces black larval phenotype in the yellow fever mosquito, Aedes aegypti

2019 ◽  
Vol 116 (43) ◽  
pp. 21501-21507 ◽  
Author(s):  
Guan-Heng Zhu ◽  
Yaoyu Jiao ◽  
Shankar C. R. R. Chereddy ◽  
Mi Young Noh ◽  
Subba Reddy Palli
Keyword(s):  
Aedes Aegypti ◽  
Larval Development ◽  
Yellow Fever ◽  
Blood Meal ◽  
Mode Of Action ◽  
Human Pathogens ◽  
Yellow Fever Mosquito ◽  
Guide Rna ◽  
Larval Stages ◽  
Blood Meal Digestion

The yellow fever mosquito, Aedes aegypti, vectors human pathogens. Juvenile hormones (JH) control almost every aspect of an insect’s life, and JH analogs are currently used to control mosquito larvae. Since RNA interference does not work efficiently during the larval stages of this insect, JH regulation of larval development and mode of action of JH analogs are not well studied. To overcome this limitation, we used a multiple single guide RNA-based CRISPR/Cas9 genome-editing method to knockout the methoprene-tolerant (Met) gene coding for a JH receptor. The Met knockout larvae exhibited a black larval phenotype during the L3 (third instar larvae) and L4 (fourth instar larvae) stages and died before pupation. However, Met knockout did not affect embryonic development or the L1 and L2 stages. Microscopy studies revealed the precocious synthesis of a dark pupal cuticle during the L3 and L4 stages. Gene expression analysis showed that Krüppel homolog 1, a key transcription factor in JH action, was down-regulated, but genes coding for proteins involved in melanization, pupal and adult cuticle synthesis, and blood meal digestion in adults were up-regulated in L4 Met mutants. These data suggest that, during the L3 and L4 stages, Met mediates JH suppression of pupal/adult genes involved in the synthesis and melanization of the cuticle and blood meal digestion. These results help to advance our knowledge of JH regulation of larval development and the mode of action of JH analogs in Ae. aegypti.

scholarly journals Storm drains as larval development and adult resting sites for Aedes aegypti and Aedes albopictus in Salvador, Brazil

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Igor Adolfo Dexheimer Paploski ◽  
Moreno S. Rodrigues ◽  
Vánio André Mugabe ◽  
Mariana Kikuti ◽  
Aline S. Tavares ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Larval Development ◽  
Aedes Albopictus ◽  
Resting Sites

THE DIGESTIVE PROCESSES OF HAEMATOPHAGOUS INSECTS: III. INHIBITION OF TRYPSIN BY HONEY AND THE POSSIBLE FUNCTIONS OF THE OESOPHAGEAL DIVERTICULA OF MOSQUITOES (DIPTERA)

1973 ◽  
Vol 105 (3) ◽  
pp. 433-436 ◽  
Author(s):  
R. H. Gooding ◽  
A. C. Cheung ◽  
B. M. Rolseth
Keyword(s):  
Ascorbic Acid ◽  
Aedes Aegypti ◽  
Proteolytic Activity ◽  
Gluconic Acid ◽  
Culex Pipiens ◽  
Culex Pipiens Quinquefasciatus ◽  
Heat Stable ◽  
Digestive Processes

AbstractHoney inhibits esterolytic and proteolytic activity of trypsin from Aedes aegypti, Culex pipiens quinquefasciatus, and cattle. The inhibitor is heat stable and only a portion of the inhibition capacity is lost from the honey by dialysis. Glucose, fructose, and sucrose have no effect upon trypsin but ascorbic acid and riboflavin are inhibitory while gluconic acid is an activator. Nectar from lilies but not from honeysuckles inhibits C. pipiens quinquefasciatus trypsin.

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