Identificación de ADN mitocondrial Bos taurus en poblaciones de ganado Cebú Brahman colombiano

El continente americano fue colonizado en el siglo XVI por los europeos quienes introdujeron por primera vez el ganado bovino de origen Bos taurus. La introducción de ganado Bos indicus ocurrió muchos años después, con las primeras importaciones desde la India, las cuales incluyeron principalmente machos. Con el fin de estudiar la participación de hembras Bos taurus en el origen del ganado Cebú colombiano, se secuenció un fragmento del ADN mitocondrial de 374 pb (D-Loop) en seis animales de la raza Cebú Brahman colombiano y 20 individuos representativos de las cinco razas criollas colombianas: seis de Blanco Orejinegro (BON), cinco de Costeño con Cuernos (CCC), tres de Romosinuano (ROMO), cuatro de Casanareño (CAS) y dos de San Martinero (SM). Adicionalmente, para el mismo fragmento se secuenciaron dos individuos de la raza española Pirenaica, como referente Bos taurus. La comparación de las secuencias reveló que los animales de la raza Cebú Brahman colombiano analizados presentaron ADN mitocondrial de origen taurino con mayor cercanía respecto de las razas criollas de origen Bos taurus europeo que con relación a las secuencias consenso Bos indicus, frente a las que se hallaron mayores divergencias. Adicionalmente, las divergencias de las razas criollas colombianas con respecto al consenso Bos taurus europeo variaron entre 0,005 y 0,014, resultado que sugiere la participación de matrilineajes Bos taurus en el origen del Cebú Brahman colombiano. Identification of mitochondrial DNA of Bos taurus origin in Colombian Zebu Brahman cattle The American continent was colonised in the XVI century by the Europeans who introduced the Bos taurus cattle. The introduction of Bos indicus cattle was done a few years later with cattle from India, mainly males. In order to study the participation of Bos Taurus females in the origin of the Colombian Zebu cattle, a 374 bp mitochondrial DNA fragment was sequenced (D-Loop) in six animals belonging to Colombian Zebu Brahman breed and 20 individuals representative of he five Colombian native breeds: 6 of Blanco Orejinegro (BON), five of Costeño Con Cuernos (CCC), three of Romosinuano (ROMO), four of Casanareño (CAS) and two of San Martinero (SM). As a reference to Bos taurus, two individuals of the Spanish Pirenaica breed were also sequenced for the same fragment. Comparison between sequences revealed that the Zebu Brahman cattle has mitochondrial DNA of Bos Taurus origin and closer to the native breeds of Spanish origin. Although described as Bos indicus, it showed the lowest genetic divergence when compared with the consensus sequence of European Bos taurus. The genetic divergences of the Colombian native breeds compared with the European Bos Taurus ranged between 0.005 and 0.014. This suggests the participation of Bos taurus matrilineages in the origin of the Colombian Zebu Brahman cattle.

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Cytogenetic and molecular analysis of the Pantaneiro cattle breed

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2006001100006 ◽

2006 ◽

Vol 41 (11) ◽

pp. 1609-1615 ◽

Cited By ~ 6

Author(s):

Érica Cunha Issa ◽

Wilham Jorge ◽

José Robson Bezerra Sereno

Keyword(s):

Mitochondrial Dna ◽

Y Chromosome ◽

Molecular Analysis ◽

Bos Taurus ◽

Bos Indicus ◽

Cattle Breed ◽

Zebu Cattle ◽

European Origin ◽

Paternal Line

The objective of this work was to characterize Pantaneiro cattle genetically through its paternal ancestry by the morphology of the Y chromosome, whether submetacentric or acrocentric, as well as to identify the maternal ancestry through mitochondrial DNA. The karyotype and mitochondrial DNA of 12 bulls of Pantaneiro breed were analyzed. The Y chromosome was analyzed in lymphocyte metaphases and the mitochondrial DNA by diagnosing its haplotype (Bos taurus and Bos indicus). Among Pantaneiro animals analyzed three had a taurine (submetacentric) Y and nine had a zebuine (acrocentric) Y chromosome, suggesting breed contamination by Zebu cattle, once Pantaneiro is considered to be of European origin. The mitochondrial DNA was exclusively of taurine origin, indicating that the participation of zebuines in the formation of the breed occurred entirely through the paternal line.

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Diversity of D-loop mitochondrial DNA (mtDNA) sequence in Bali and Sumba Ongole cattle breeds

Journal of the Indonesian Tropical Animal Agriculture ◽

10.14710/jitaa.44.4.335-345 ◽

2019 ◽

Vol 44 (4) ◽

pp. 335

Author(s):

J. Jakaria ◽

T. Musyaddad ◽

S. Rahayu ◽

M. Muladno ◽

C. Sumantri

Keyword(s):

Mitochondrial Dna ◽

Genetic Distance ◽

Bos Taurus ◽

Bos Indicus ◽

Haplotype Diversity ◽

Complete Sequence ◽

Distance Method ◽

Mtdna Sequence ◽

D Loop ◽

Bali Cattle

This study aimed to investigate the diversity of the complete sequence of D-loop mitochondrial DNA (mtDNA) in Bali and Sumba Ongole (SO) cattlebreeds. A total of 24 blood samples were collected from Bali cattle (19 heads) and SO cattle (5 heads), and were extracted and then analyzed to obtain the sequence of D-loop mt DNA.Multiple alignments of the whole sequence of D-loop mtDNA were determined using clustal W. Genetic distance was calculated using a p-distance method, while the genetic tree was constructed using neighbor-joining (NJ) based on MEGA 6. Haplotype number, haplotype diversity (Hd) and nucleotide diversity (Pi) were analyzed using DnaSP version 6. As a result, the sequence of D-loop mtDNA in Bali cattle (921-1119 bp) and SO cattle (913 bp) was reported to have 8 and 4 haplotypes. Hd and Pi of Bali cattle reached 0.625±0.139 and 0.0266±0.0145, respectively, which wwere different from that of SO cattle, namely 0.900±0.1610 and 0.0064±0.0015, respectively. Specifically, we found 22 bp-repetitive nucleotide in Bali cattle, existing 3-9 times with a length of 66-198 bp present in D-loop mtDNA. This unique feature did not exist in SO cattle. Genetic distance and genetic tree determined according to sequence in hypervariability (HV-1) region of D-loop mtDNA (166 bp) resulted in satisfied separation, successfully classifying Bos javanicus, Bos indicus, and Bos taurus cluster.

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GENEBANK ANALYSIS: SINGLE NUCLEOTIDE POLYMORPHISMS OF ANIMALS MITOCHONDRIAL GENOME UKRAINIAN GRAY AND UKRAINIAN WHITEHEAD CATTLE BREEDS

Animal Breeding and Genetics ◽

10.31073/abg.53.33 ◽

2017 ◽

Vol 53 ◽

pp. 241-248

Author(s):

Yu. V. Podoba ◽

V. O. Pinchuk ◽

V. P. Boroday

Keyword(s):

Mitochondrial Dna ◽

Mitochondrial Genome ◽

Dna Sequences ◽

Bos Taurus ◽

Bos Indicus ◽

Ex Situ ◽

Single Nucleotide ◽

Cattle Breeds ◽

D Loop ◽

Mtdna Haplogroup

Examination of variation in mitochondrial DNA (mtDNA) control region sequences has been pivotal in the elucidation of bovine phylogeography. Initial studies have demonstrated a deep bifurcation in bovine mtDNA phylogeny, which indicates a predomestic divergence between the two major taxa of cattle, humped zebu (Bos indicus) and humpless taurine (Bos taurus). Subsequent genetic investigations have yielded further inference regarding origins within the B. taurus lineage. B. taurus mtDNA sequences fall into one of five ancestral star-like haplotypic clusters, which are geographically distributed. Just one of these clusters, T3, predominates in Western Europe. Symmetrically, diversity within Africa is composed almost exclusively of members of a separate haplotypic cluster, T1, which is rarely detected elsewhere. The almost mutually exclusive geographic distribution of these two haplotypic clusters allows geographical exceptions to be securely identified as secondary introductions. We investigated a comparative analysis of mitochondrial genome sequences for different breeds of cattle (Bos taurus, Bos indicus) with global genetic bank. Mitochondrial DNA sequences from bovine animals (Bos taurus) breeds Ukrainian Whitehead and Ukrainian Gray freely available on the global genetic bank (http://www.ncbi.nlm.nih.gov/Genebank/). Local alignment of sequences for mitochondrial genome of different cattle breeds was performed using the program MEGA 4.0. For the detection of nucleotide replacements used mitochondrial DNA sequence of Bos taurus Hereford breed (Anderson S. at al., 1982) as a reference (accession number V00645). Here we report the analysis results of testing for 9 genotypes Ukrainian Gray mitochondrial DNA sequences showed that one animal (GQ129208) has haplotype Bos indicus, other belongs to haplogroup T1 with European origin mtDNA. Analysis of single nucleotide replacement in one of the hypervariable regions mtDNA (position number 16019-16339) shows, that among 10 submitted genotypes of Ukrainian Whitehead the 3 of them (FJ014303, FJ014298, FJ014294) relating to T1a mtDNA haplogroup of African origin, which characterized by replacement of T to C at position 16255. Also have been two animals (FJ014301, FJ014295) with single nucleotide replacements with relatives to Bos indicus mtDNA haplogroup. We performed alignment with reference sequences (Bos_taurus_v00654.1) and comparative nucleotide sequences analysis of another hypervariable D-loop (position number 1-240) mtDNA with 5 Ukrainian Whitehead genotypes and 5 Ukrainian Gray genotypes represented in genetics bank. Among the Ukrainian Whitehead genotypes (FJ014298, FJ014297, FJ014296, FJ014295, FJ014294) all were polymorphic that characterizes large differentiation these animals for maternal and describe deep heterogeneous parent population of studied group. We determined one animal with genotype FJ014295 was significantly different by the number of segregation sites. The analyzed sequences (FJ014290, FJ014289, FJ014288, FJ014287, FJ014286) of 5 Ukrainian Gray genotypes showed no polymorphism in hypervariable D-loop (position number 1-240) mtDNA. The mtDNA analysis of different species of animals allowed to distribute their mtDNA belonging to European, African and Asian haplogroups. The technique, which allows to differentiate the animals represented by their belonging to the respective haplogroups. The process that gave rise to different genotypes in one lineage is clearly of fundamental importance in understanding intraspecific mitochondrial polymorphism and evolution in mammals. Сomprehensive study genetic material provide more opportunities to optimize costs in-situ conservation of different cattle breeds, to optimize methods and techniques which used in ex-situ conservation programmes of National gene bank of animal genetic resources.

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Differences in copper and selenium metabolism between Angus (Bos taurus) and Brahman (Bos indicus) cattle

Journal of Animal Science ◽

10.1093/jas/skab048 ◽

2021 ◽

Author(s):

J Ranches ◽

R Alves ◽

M Vedovatto ◽

E Anne Palmer ◽

P Moriel ◽

...

Keyword(s):

Bos Taurus ◽

Bos Indicus ◽

First Trimester ◽

Beef Cows ◽

Experimental Unit ◽

University Of Florida ◽

Brahman Cattle ◽

Research And Education ◽

The University ◽

Se Status

Abstract A two-year study was conducted at the University of Florida – IFAS, Range Cattle Research and Education Center (Ona, FL) to evaluate differences in the metabolism of Cu and Se of Angus (Bos taurus) and Brahman (Bos indicus) cattle. Thirty-two pregnant beef cows (n = 8 Brahman and 8 Angus/year) were enrolled in the study in the first trimester of gestation. The study consisted of 3 phases: (1) restriction (d 0 to d 90); (2) supplementation (d 91 to 150), and (3) calving. During all 3 phases, cows were individually fed and housed in partially covered drylot pens. During the restriction and supplementation phases cows were provided a 1.5 kg/d of a grain-based concentrate supplement, which was fortified with flowers of S (50 g of supplemental S/cow daily; restriction phase) or Cu and Se (100 and 3 mg/d of Cu and Se, respectively; supplementation phase). Blood and liver samples were collected from all cows on 30 d intervals and from both cows and calves within 24 h of calving. Colostrum and milk samples were collected at calving and 7 d after birth. All data were analyzed using the MIXED procedure of SAS, where cow and calf were the experimental unit. During the restriction phase, a breed × day effect (P = 0.03) was observed where Brahman had greater liver Cu concentration than Angus cows in all sampling days. For liver Se concentration, a tendency (P = 0.07) for a breed effect was observed where Angus cows tended to have greater liver Se concentration than Brahman. During the supplementation phase, breed (P < 0.001) and day (P < 0.01) effects were observed, where Brahman cows had greater liver Cu concentration than Angus. For liver Se concentration, a day effect (P < 0.001) was observed, where liver Se concentration increased (P < 0.001) from d 90 to 120 and remained unchanged (P = 0.86) until d 150. At calving no effects of breed (P = 0.34) were observed for liver Cu concentration of cows, however, Brahman calves tended (P = 0.09) to have greater liver Cu concentration than Angus calves. For Se liver concentration at calving, Angus cows tended (P = 0.07) to have greater liver Se concentration than Brahman cows, however no breed differences (P = 0.70) were observed for liver Se concentration of calves at birth. In summary substantial differences in multiple indicators of Cu and Se status were observed between Angus and Brahman cattle, implying that Angus and Brahman cattle possibly have different mechanisms to maintain adequate Cu and Se status.

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T-Cell and Antibody Responses to Mycobacterial Antigens in Tuberculin Skin-Test-PositiveBos indicusandBos taurusCattle in Ethiopia

Veterinary Medicine International ◽

10.1155/2012/457872 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6 ◽

Cited By ~ 3

Author(s):

Gobena Ameni ◽

Paul Cockle ◽

Konstantin Lyashchenko ◽

Martin Vordermeier

Keyword(s):

T Cell ◽

Skin Test ◽

Bos Taurus ◽

Bos Indicus ◽

Antigen Recognition ◽

Antibody Responses ◽

Zebu Cattle ◽

Mycobacterial Antigen ◽

Multiple Antigen ◽

Mycobacterial Antigens

Higher IFN-γresponses to mycobacterial antigens were observed inBos taurus(Holsteins) than inBos indicus(Zebu) cattle which could due to differences in antigen recognition profiles between the two breeds. The present study was conducted to evaluate mycobacterial antigen recognition profiles of the two breeds. Twenty-three mycobacterial antigens were tested on 46 skin test positive (24 Zebu and 22 Holstein) using enzyme-linked immunospot assay (ELISPOT) and multiple antigen print immunoassay (MAPIA). Herds from which the study cattle obtained were tested for Fasciola antibody. The T cells from both breeds recognized most of the mycobacterial antigens at lower and comparable frequencies. However, antigens such as CFP-10, ESAT-6, Rv0287, Rv0288, MPB87, Acr-2, Rv3616c, and Rv3879c were recognized at higher frequencies in zebu while higher frequencies of T cell responses were observed to Hsp65 in both breeds. Furthermore, comparable antibody responses were observed in both breeds; MPB83 being the sero-dominant antigen in both breeds. The prevalence of Fasciola antibody was 81% and similar in both breeds. This piece of work could not lead to a definitive conclusion if there are differences in mycobacterial recognition profiles between the two breeds warranting for further similar studies using sound sample size from the two breeds.

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Productivity of European cattle [Bos taurus], Zebu cattle [Bos indicus] and their crosses kept at the Llanos of Venezuela. I. Reproductive efficiency.

Theriogenology ◽

10.1016/0093-691x(75)90165-x ◽

1975 ◽

Vol 3 (3) ◽

pp. 123

Author(s):

W. Jöchle

Keyword(s):

Bos Taurus ◽

Bos Indicus ◽

Reproductive Efficiency ◽

Zebu Cattle ◽

European Cattle

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Bos indicus or Bos taurus mitochondrial DNA - comparison of productive and reproductive breeding values in a Guzerat dairy herd

Genetics and Molecular Research ◽

10.4238/vol7-3gmr449 ◽

2008 ◽

Vol 7 (3) ◽

pp. 592-602 ◽

Cited By ~ 11

Author(s):

J.C.C. Paneto ◽

J.B.S. Ferraz ◽

J.C.C. Balieiro ◽

J.F.F. Bittar ◽

M.B.D. Ferreira ◽

...

Keyword(s):

Mitochondrial Dna ◽

Bos Taurus ◽

Bos Indicus ◽

Dairy Herd ◽

Breeding Values

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Whole genome characterization of autochthonous Bos taurus brachyceros and introduced Bos indicus indicus cattle breeds in Cameroon regarding their adaptive phenotypic traits and pathogen resistance

10.21203/rs.2.20033/v1 ◽

2020 ◽

Author(s):

Archile Eric paguem ◽

Babette Abanda ◽

Mbunkah Daniel Achukwi ◽

Praveen Baskaran ◽

Stefan Czemmel ◽

...

Keyword(s):

Bos Taurus ◽

Bos Indicus ◽

Cattle Breed ◽

Phenotypic Traits ◽

Zebu Cattle ◽

Whole Genome ◽

Genetic Traits ◽

Cattle Breeds ◽

Taurine Breed ◽

Taurine Cattle

Abstract BackgroundWest African indigenous taurine cattle display unique adaptive traits shaped by husbandry management, regional climate and exposure to endemic pathogens. They are less productive with respect to milk and meat production which has been associated with a number of factors, amongst others small size, traditional beliefs and husbandry practices. This resulted in the severe dwindling of their populations size rendering them vulnerable to extinction. The Namchi (Doayo) taurine cattle breed has documented resistance traits against trypanosome infection and exposure to tick infestation. Nonetheless, the historically later introduced Zebu cattle are the main cattle breeds in Africa today, even though they suffer more from locally prevailing pathogens. By using a reference-based whole genome sequencing approach, we sequenced for the first time the genomes of five cattle breeds from Cameroon: the Namchi (Doayo), an endangered trypanotolerant taurine breed, the Kapsiki, an indigenous trypanosusceptible taurine breed, and three Zebu (Bos indicus indicus) breeds: Ngaoundere Gudali, White Fulani and Red Fulani.ResultsApproximately 167 Giga bases of raw sequencing data were generated and mapped to the cattle reference genome UMD3.1. The coverage was 22 to 30-fold. The single nucleotide polymorphisms (SNPs) were compared with reference genomes of European Bos taurus Holstein and of Asian Bos indicus Brahman and the African trypanotolerant N’Dama breeds.Of a total of 50 million SNPs identified, 3.43 million were breed-specific ranging from 0.37 to 0.47 million SNPs in the domestic Cameroonian breeds and approximately 0.58 million constituted of small insertions and deletions. We identified breed specific-non-synonymous variants as genetic traits that could explain certain cattle-breed specific phenotypes such as increased tolerance against trypanosome parasites in the Namchi (Doayo) breed, heat tolerance in the Kapsiki breed, and growth, metabolism and meat quality in the Gudali breeds. Phylogenetic comparison grouped Namchi (Doayo) to the African Zebu clade indicating a hybrid status of the selected animal with a Zebu breed, albeit it showed the Namchi breed’s phenotype.ConclusionsThe findings provide the first comprehensive set of full genome variant data of the most important Cameroonian cattle breeds. The genomic data shall constitute a foundation for breed amelioration whilst exploiting the heritable traits and support conservation efforts for the endangered local cattle breeds.

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Phenotypic characterization of the Saudi Arabian Hassawi cattle breed

Animal Genetic Resources Information ◽

10.1017/s1014233900000900 ◽

1997 ◽

Vol 21 ◽

pp. 35-42 ◽

Cited By ~ 4

Author(s):

T. A. Mohammed

Keyword(s):

Bos Taurus ◽

Bos Indicus ◽

Cattle Breed ◽

Farming System ◽

Phenotypic Characterization ◽

Zebu Cattle ◽

Body Conformation ◽

High Feed ◽

Farming Families

SummaryHassawi cattle breed is a mix of Bos indicus and Bos taurus. The cattle are raised in the Eastern province of the country by farming families in mixed farming system. The breed numbers are declining very fast, from 10 449 head in 1986 to an estimated maximum of 4 500 head at present.The decrease is mainly due to replacement by exotic breeds, the indiscriminate crossing with these exotics, particularly in view of the scarcity of the Hassawi bulls for mating. Animals are small in size, mature body weight 210-270 kg for bulls and 150-200 kg for cows, quite uniform in colour (light red) and body conformation have conspicuously reduced dewlap and umbilical folds and relatively large hump. Animals are heat tolerant, sustain high feed intake under ambient temperature, resistant to many diseases prevailing in the region and cows have good mothering ability. Productivity of the breed in terms of meat and milk is low when compared to that of exotics in high input production environments, but reproduction performance excels that of temperate breeds and zebu cattle.Efforts should be made to stop the decline in the breed numbers and to conserve the breed as an asset for production under harsh environment.

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