The economic efficiency of the use of mixed ligand complexes of Zinc, Manganese and Cobalt in the rations of highlyproductive cows of the Ukrain-ian Red-Spotted Dairy breed

The article deals with the results of research on the economic efficiency of the use of mixed ligand complexes of Zinc, Manganese and Cobalt in the feeding rations of highlyproductive cows of Ukrainian Red-Spotted Dairy breed in the first 100 days of lactation. The research was performed on five groups (one control and four experimental) of analogous cows in the conditions of ALC “Terezyne” Bila Tserkva district of Kyiv region. The control was the optimal dose of mixed ligand complexes of Zinc, Manganese and Cobalt, which was previously established with a concentration of 1 kg of dry matter (DM) of the feed mixture (FM), mg: Zinc – 60.8; Manganese – 60.8 and Cobalt – 0.78. For the 2nd experimental group, the concentration of these trace elements was increased by 10 %, and in the 3rd, 4th and 5th experimental groups – on the contrary, the concentration of Zinc, Manganese and Cobalt in 1 kg of DM of feed mixture was reduced by 10 %, 20 and 30 %, respectively, compared with the control. The best results on milk yield of natural and basic fat content were got from cows of the 4th experimental group, where the concentration of Zinc, Manganese and Cobalt due to their mixed ligand complexes in 1 kg of DM of feed mixture was, mg: Zinc – 48.6; Manganese – 48.6; Cobalt – 0.62. The cost of products got from experimental cows ranged from UAH 12702.3 up to UAH 13783.7 and it was the highest in the 4th experimental group. It was set up that for cows of Ukrainian Red-Spotted Dairy breed in the first 100 days of lactation the most economically advantageous was the dose of mixed ligand complexes, in which the concentration of Zinc and Manganese was 48.6 mg, and Cobalt – 0.62 mg/kg DM, which allowed to obtain a profit of 14.37 % more than in the control group. Doses of mixed ligand complexes with a concentration of Zinc and Manganese – 66.9 mg; 54.7 and 42.6 mg, and Cobalt – 0.86 mg; 0.7 and 0.55 mg/kg CF gave a much lower economic effect. Additional profit in the 2nd experimental group amounted to UAH 66.8 or 1.34 %; in the 3rd – UAH 95.6 or 1.92 % and in the 5th – UAH 561.6 or 11.28 %, compared with the control group.

Fattening indicators of young rabbits when feeding Prebiolact-Kr

Measures to increase production are used to address the issue of providing the population with food. During the peak of the industry (1975–1985), Ukraine annually produced 120–165 thousand tons of rabbit meat, 30–45 million skins, and its share in world rabbit production reached 8 %. In the national volume of meat production, the share of highly dietary rabbit accounted for 8–10 %. Rabbit breeding in Ukraine was a very lucrative industry, which employed more than 600 thousand rabbit breeders. However, in recent years, rabbit breeding has declined due to the lack of modern scientific developments on the issues of complete feeding, rabbit keeping technology, low level of breeding and veterinary support. The development of the industry and the lack of coordinating bodies, both in the center and on the ground, are holding back. At present, the rabbit population of Ukraine is 97–98 % concentrated in individual farms and is 1.2–1.3 million breeding and repair livestock, and the rest in farms and breeding entities of various forms of ownership and management. According to the results of state certification, the breeding base of the industry in Ukraine today consists of 1 breeding plant and 7 breeding breeders of rabbit breeds. For feeding young rabbits used complete ration granular feed, the structure of which had different prebiotic content: I control group – the drug was not received, 2 experimental – 1.5, 3 – 2.0, 4 – 2.5 g/goal. per day. The studies were conducted on four groups of young rabbits, selected on the principle of groups of analogues, 25 heads in each. Thus, the total amount of feed increased from 80 g/goal. per day (35–41 days), up to 100 g (42–48 days), 120 g (49–55 days), up to 140 g (56–62 days), up to 160 g (63–69 days) up to 180 g (70–76 days). Feeding of young rabbits when reared for meat Prebiolact-Kr in animals of the second group at 43.1 g, the third 44.6 g and 45.0 g. The use of Prebiolact-Kr in the feeding of young rabbits when fed meat contributes to an increase in fattening performance, which may be the basis for the practical application of this supplement. Perebiolakt-KR showed the best results in feeding fattening and repair young animals at a dose of 2.0 g/goal per day.

Influence of alimentary factors on the absorption of feed nutrients and poultry productivity

The article is a summary of the literature on the main aspects of the modern bird feeding system. Theoretical aspects and results of experimental research of scientists on the substantiation of parameters of protein and amino acid nutrition are given, which is an important factor that, under appropriate conditions can significantly guarantee the realization of the genetic potential of corresponding breeds and crosses of poultry. It is shown that an important aspect of protein nutrition is the ratio of feed amino acids. Their negative interaction can be caused by a deficiency of one or more amino acids, an imbalance between them, antagonism, and toxicity. This is accompanied by effects on various physiological and biochemical processes, significantly affects appetite, intestinal absorption, renal reabsorption and transport of amino acids, their catabolism, rate of protein decomposition, synthesis, and formation of toxic metabolic products. The data show that the required level of energy for the bird's body is provided by carbohydrates (mainly of plant origin) and lipids (fat supplements of various origins). It is noted that lipids promote the absorption, transport, and deposition of fat-soluble vitamins. The effectiveness of using fat supplements as energy depends on their source. At the same time, among the indispensable nutrients that have a significant impact on growth intensity, reproductive quality, poultry productivity, and biological value of products, an important role is played by macro-and micronutrients. Many researchers emphasize that along with the use of traditional feeds in poultry feeding, the possibilities of non-traditional cereals rich in protein and lipids are not fully used. A limiting factor in their use is the presence in their composition of anti-nutrients contained in many feeds. Once in the digestive tract of animals, they negatively affect the absorption of feed nutrients. In most cases, the consumption of such feed by animals is manifested in growth retardation, increased feed consumption, hormonal effects, and, less frequently, in the dysfunction of certain organs.

Relative variability of phenotypic traits and indicators of milk productivity of cows

The research was conducted on cattle of Black-and-White breed (Holstein and Ukrainian Black-and-White dairy breed) at LLC “Veleten” Glukhiv district of Sumy region (n = 1956) and PJSC “Breeding Plant “Stepnoy” of Kamyansko-Dniprovsk district of Zaporizhzhia region (n = 1981). Based on retrospective data analysis (“Uniform-Agri” – program of dairy herd management) for the period from 2004 to 2017 it was studied the live weight of cows in growing period (newborns, 6, 12; 18 months, at first insemination and after the first calving), body measurements of heifers (height at shoulders, chest depth, chest width, chest girth behind the shoulder blades, oblique body length, width of hips, wrist girth) and traits of reproducibility (age at first insemination, age at first calving, duration of service period, insemination index, fertility index) and milk productivity of cows (milk yields for 305 days, fat and protein content in milk for the first, second and third lactation). Based on our previously obtained data by correlation analysis method it was studied the relative variability of phenotypic traits and indicators of dairy productivity of cows. It is established that the connections of live weight of cows during their rearing with traits of milk productivity were multidirectional and ranged from slightly improbable to moderately significant values. At the same time, in both farms, the most significant highly probable connection was noted between the live weight of animals at the age of 12 months and the milk yield of first-borns that indicates the possibility of selecting heifers at the specified age to provide them future high milk productivity. There was significant variability of the relationship between body measurements and the amount of milk yield of cows for the first, second and third lactation. However, cows of both farms, had direct connection, but a bit weaker in animals at PJSC “Breeding Plant “Stepnoy”. Positive correlation coefficients between indicators of exterior and milk yields of cows give reasons to claim the efficiency of indirect selection of heifers by body measurements. Rational management of dairy farming and high profits depend a lot on knowledge of regularities of correlative variability of productive traits of cows and indicators of reproductive capacity. It is established that the connections between the traits of milk productivity and reproductive capacity of animals were different and ranged from weak to medium and from reliable to improbable. The most significant highly reliable, however, with negative correlation coefficients were observed between milk yield of cows for all studied lactations and their age at the first insemination and the first calving

Monitoring of productive and biochemical parameters of young Clarias gariepinus during feeding of Skretting and Roycher AQUA feeds in experimental conditions

The article presents the results of monitoring the productive and biochemical indicators of the growth rate of young clary catfish using dry extruded complete feed for fish, which have proven to be best adapted for use at all stages of growing clary catfish, namely: Skretting and Roycher AQUA in experimental conditions. The research was conducted in the educational and experimental laboratories of Bila Tserkva National Agrarian University from 2019 to 2021. For the experiment, we used the young of the African marble clary catfish (Clarias gariepinus), purchased from one of the fish farms in the Odesa region. Clary catfish fry was kept in four rectangular plastic containers for 90 liters of freshwater. The tanks were two-thirds full (60 liters). The water temperature was maintained at 26 °C. For the experiment, 120 fries were used, which were kept in four plastic tanks by the method of analogs of 30 specimens each. Given that the fry differed in weight (for experiment 1 we selected fry with higher weight, and for experiment 2 – with lower weight), they were divided on the principle of analogs into two experimental and two control groups. Based on the results of monitoring in Experiment 1, fish observations, and mathematical calculation, we found that the feed ratio when fed Skretting and Roycher AQUA starter feed is 0.74 and 0.99 %, respectively. Based on the results of monitoring, fish observations, and mathematical calculation, it was found that the feed ratio in experiment 2 when feeding starter feed Skretting and Roycher AQUA is 0.75 and 1.54 %, respectively. The high feed rate for the use of Roycher AQUA can be explained by overuse due to the grinding of the granules. Growing aquaculture facilities in an artificially formed system – production, allows you to get environmentally friendly fish products all year round. This is a very important criterion in modern environmental conditions.

Influence of early spring feeding on the development of bee families in the natural lands of Polissya

To ensure intensive early spring development of bee colonies in the natural lands of Polissya Zhytomyr region in the winter-spring period it is necessary to feed stimulating food. Therefore, the aim of the work is to study the influence of spring feeding with sugar-honey dough on the development and honey productivity of bee families of Ukrainian breed in conditions of clean and radioactively contaminated natural lands of Zhytomyr Polissya. The influence of feeding bee colonies with sugar-honey dough (powdered sugar, centrifuged honey, water) using pure and radioactively contaminated honey on the intensity of development and honey productivity was studied. To do this, two groups were created with experimental families of Ukrainian bee breeds, selected on the principle of analogs, one of which was in a radioactively clean area, and the other in radioactively contaminated soils 137 Cs within 15 Ki/km² and above. A comparative assessment of the early spring development of families was made: from March 16 to May 20, with an interval of 21 days, the number of open and sealed brood, egg production of queens during the honey harvest season was determined. Honey productivity at the honey harvest from natural lands was determined by the amount of honey obtained in spring and summer. Early spring feeding with sugar-honey dough promotes intensive development of families, and the content of radioactively contaminated honey does not have a negative impact on their development. After the end of the season of honey collection from natural lands, bee families of Ukrainian breed, both in the radioactively contaminated and clean zone, had a medium strength and could successfully overwinter.

Correlations between microsatellite DNA heterozygosity and reproductive traits in Large White sows

The main aim of this paper was to determine whether heterozygosity (assessed using microsatellite genotypes) was correlated with the reproductive traits in sows. The study was conducted on two herds of sows of the Large White sows breed at the Limited Liability Company “Tavriys’ki svyni” (Kherson region, Ukraine) and the Agricultural Private Enterprise “Techmet-Yug” (Mykolayiv region, Ukraine). During the study, we used eleven microsatellite loci recommended by International Society for Animal Genetics (ISAG) – S0101, S0155, S0228, S0355, S0386, Sw24, Sw72, Sw240, Sw857, Sw936 and Sw951. The litter records included information on the total number of piglets born (TNB), number of piglets born alive (NBA), number of stillborn piglets (NSB), frequency of stillborn piglets (FSB), litter size at weaning (NW) in the first five parities. Individual heterozygosity estimates (for each microsatellite loci separately) and microsatellite multilocus heterozygosity (for all used loci) estimates (MLH) were used in our analysis. ANOVA was used to determine the relationship of the dependent effects (reproductive traits) to single locus heterozygosity using two classes: 0 (for homozygous individuals) and 1 (for heterozygous individuals). In addition, the following indicators were calculated for each genotype: the squared distances (d2) between alleles within an individual for each microsatellite loci and mean squared distances (mean d2) between alleles within an individual for 11 microsatellite loci. Spearman’s rank correlation coefficients were used to measure the association between d2 (for each microsatellite loci) and reproductive traits in sows. ANOVA on reproductive traits of sows belonging to different MLH and mean d2 classes was also undertaken. For sows from the Agricultural Private Enterprise “‘Techmet-Yug” were observed negative associations between heterozygosity and reproductive traits. We conclude that care should be taken when crossing between different breeders (English and Hungarian selection) to avoid outbreeding depression.

Quality control of butter

The most common problem among ready-made foods is the adulteration of butter, which is replaced by margarine or vegetable spreads. Milk and other dairy products made from raw milk are not left out. The production and sale of low-quality, counterfeit products poses a direct threat to human health and affects the competitive environment among producers. As a result of research, it should be noted that the raw milk market is not fully saturated, so improving the quality of cream production and processing of milk products, namely butter, competition for suppliers, microbiological improvements, as well as improvements to plants and systems – remains relevant. During the analysis, some physicochemical parameters of butter were studied, namely: acidity of the fat phase, mass fraction of moisture and fat. The acidity index was within the norm, although it differed in all samples: the lowest was found in sample № 1 (1.1 °K), and the highest was in sample № 2 (1.6 °K). As for such an indicator as the content of table salt and the content of fat and pH of plasma, the oils Sweet cream “Selyanske”, 72,6 % TM “Svoya Liniya”, Sweet cream, 73.0 % LLC “Eney”, “Poltavske”, 62.5 % LLC “Techmolprom” do not meet the requirements of DSTU 4399:2005. The mass fraction of fat in butter brands PJSC “Dubnomoloko” and “Pryvat-Fud” – was lower than indicated on the package, by 1.4 % and 2.6 %, respectively, and brands “Svoya Liniya”, LLC “Eney” and Techmolprom LLC on the contrary – by 18.0 %, 18.4 % and 12.5 % more. According to the results of microbiological studies, it was found that the number of mesophilic aerobic and facultatively anaerobic microorganisms, yeasts, fungi and bacteria of the Escherichia coli group in the studied samples of butter of all brands does not exceed the permissible norms. This fact testifies to the relative “purity” of the raw material from which the butter was made, as well as to the observance of veterinary and sanitary and hygienic norms during the production and storage of butter. Summing up the research, it should be noted that, despite the identified physical and chemical shortcomings of all samples of butter, preference should be given to sour cream butter “Selyanske”, 73.0 % of PJSC “Dubnomoloko” and “Selyanske” sweet cream, 73.0 % “Privat- Fud”, as none of them exceeded such indicators as fat content and plasma pH of oil, which may indicate the absence of impurities in vegetable fats in this product.

The effect of the drug “Amiksyn” on the immune status of carp

The productivity of pond fish, in particular carp, largely depends on the intensity of use of aquatic bioresources. The aim of the study was to investigate the effect of “Amiksyn” on the immunological parameters of carp fish, namely: the number of T- and B-lymphocytes, immunoglobulins, the level of circulating immune complexes, lysozyme, phagocytic activity and bactericidal activity of serum. 10 fish were selected for hematological and biochemical studies. The research was conducted in the aquarium of the Department of Aquatic Bioresources of Stepan Gzhytskyi NUVMB Lviv. Fish were kept in the pool for 21 days to adapt to the new conditions, and at the beginning of the series of experiments were placed in aquariums with a volume of 200 liters. Aeration and mechanical filtration of water were provided in the tanks. The temperature during the experiments fluctuated slightly and was 18 ± 1.5 °C. The main hydrochemical parameters corresponded to fish farming standards. In order to adjust the immune response, and thus prevent possible manifestations of fish damage by pathogens, we studied the possibility of using the immunostimulant “Amiksyn”, which was used for 5 – 10 – 15 – 20 days and then dosing 5 – 10 – 15 mg/kg body weight Pisces. The use of “Amiksyn” at a dose of 10 mg/kg of fish weight four times during the fifteen-day period with an interval of 5 days provided a decrease in the number of T-lymphocytes by 9.1 % and B-lymphocytes by 23.0 %; caused the activation of non-specific resistance indicators, namely lysozyme activity increased by 28.7 %, phagocytic activity of leukocytes increased by 14.0 % and bactericidal activity of serum increased by 19.0 %. Therefore, to adjust the immune system and stimulate the metabolic processes of fish in order to prevent the negative effects of various pathogens, the most optimal dose was 10 mg/kg of fish weight within 15 days of use.

Assessment of the quality of the southern Bug waters within the Khmelnytsky region and biotechnological ways to solve their purification

Water resources are the national wealth of each state, an important natural resource, and determine the development opportunities of most sectors of the economic complex of Ukraine. The Southern Bug River is the largest river, the basin of which is completely located in Ukraine. Due to the intensive growth of anthropogenic impact on the studied reservoir and the economic use of its resources, the quality of water in it has significantly decreased. To improve the quality of water in rivers, it is optimal to use biotechnological methods, which provide many opportunities for effective solutions to issues related to monitoring, assessment, and treatment of polluted watercourses. The aim of our study was to assess water safety, the current ecological status of the Southern Bug River within the Khmelnytsky region in three areas (Maryanivske Reservoir, Kopystyn, Shchedrivske Reservoir) and use in domestic needs, with analysis of potential risks to human health, development of new biotechnological and improvement of existing measures to improve water supply. The experimental part of the work was performed on the basis of the Khmelnytsky Regional Department of Water Resources in the laboratory at the address Khmelnytsky, st. Soborna, 29. We conducted a chemical assessment of the Southern Bug River on three indicators: salt composition (I1), tropho-saprobiological (I2), and specific toxic substances (I3). Research of river waters was carried out in order to control the MPC (maximum permissible concentrations) of substances for use in household and food needs of the population and to propose biotechnological methods of river water purification within the Khmelnytsky region. According to our research on hydrochemical characteristics, the water contained elevated levels of NH4, NO2, NO3, PO4, the average values of HSC (chemical oxygen demand) exceeded the MPC in all areas by 2 to 5 times. This may be due to exposure to organic compounds of natural origin. The content of nitrites, nitrates is within normal limits, but the content of ammonium salt exceeds the MPC up to 20 times in the village Kopystyn and from 2 to 7 times in the village of Letychiv. According to many indicators of the salt block, the maximum concentration limit was not exceeded, except for the village Kopystyn, the concentration of Magnesium (Mg) was 84.33 mg/dm3 at a rate of 40.00 mg/dm3. According to specific toxic substances, the water belonged to the III class 4 quality category, Chromium (Cr) was not detected, Manganese (Mn) and Copper (Cu) were within normal limits, although the Copper content was high. The concentration of Iron (Fe) varies in different areas and during the year, there is a case of excess Iron in the village. Kopistin up to 0.86 mg/dm3 at a rate of 0.30 mg/dm3. Iron affects the intensity of phytoplankton development and the qualitative composition of the microflora, so its concentration is subject to seasonal fluctuations. To improve the quality of water in the Southern Bug River, we have proposed the use of biotechnological methods, including those that provide many opportunities to effectively address issues related to monitoring, assessment, and treatment of polluted watercourses.