scholarly journals Monitoring the viability of citrus rootstocks seeds stored under refrigeration

2013 ◽  
Vol 35 (1) ◽  
pp. 238-245 ◽  
Author(s):  
Sérgio Alves de Carvalho ◽  
Luis Fernando Carvalho Silva

The citrus nursery tree is produced through the bud grafting process, in which rootstock is usually grown from seed germination. The objective of this research was to evaluate, in two dissimilar environmental conditions, the viability and polyembryony expression of five citrus rootstocks seeds stored in different periods under refrigeration. The rootstock varieties evaluated were: Rangpur lime (Citrus limonia Osb. cv. Limeira), Trifoliate orange (Poncirus trifoliata Raf. cv. Limeira), Citrumelo (P. trifoliata x C. paradisi Macf. cv. Swingle), Sunki mandarin (C. sunki Hort. ex Tanaka) and Volkamer lemon (C. volkameriana Ten. & Pasq. cv. Catania 2). The experimental design was the randomized blocks in a 11 x 5 x 2 factorial scheme, evaluating from time zero to the tenth month of storage, the five varieties of rootstock in two environments: germination and growth B.O.D type chamber (Biological Oxygen Demand - Eletrolab Brand Model FC 122) at 25 °C; and greenhouse seedbed with partial temperature control (22 °C to 36 °C) and humidity control (75-85%). The plot had 24 seeds in four replicates, using trays with substrate in greenhouse and Petri dishes with filter paper in B.O.D. chamber. The seed germination rate and polyembryony expression were evaluated monthly. It was concluded that Trifoliate and Citrumelo Swingle seeds can be stored for up to seven months, while Volkamer lemon, Rangpur lime and Sunki seeds can be stored for up to ten months. The polyembryony expression rate was slightly higher when measured in greenhouse than in B.O.D. chamber and remained stable in both environments until the seventh month, from which dropped sharply. Citrumelo Swingle seeds expressed the highest polyembryony rate (18.8%), followed by Rangpur lime and Volkamer lemon (average value of 13.7%), Sunki (9.4%) and Trifoliate (3.2%). Despite some differences among varieties, the viability of rootstock stored seeds can be monitored either in the greenhouse or in B.O.D. germination chamber, the latter being the faster and more suitable method.

2004 ◽  
Vol 61 (2) ◽  
pp. 151-155 ◽  
Author(s):  
Neusa Maria Colauto Stenzel ◽  
Carmen Silvia Vieira Janeiro Neves

The 'Tahiti' lime (Citrus latifolia Tanaka) is an important commercial citrus cultivar in Brazil. 'Rangpur' lime has being used as its main rootstock, but it is susceptible to root rot caused by Phytophthora, reducing tree longevity. An experiment was set up in a randomized block design, with three trees per plot of each rootstock and four replicates, and run for 12 years, aiming to compare the performance of 'IAC-5 Tahiti' lime, budded on 'Rangpur' lime (Citrus limonia Osb.); 'C-13' citrange (Citrus sinensis (L.) Osb. × Poncirus trifoliata (L.) Raf.); 'African' rough lemon (Citrus jambhiri Lush.); 'Volkamer' lemon (Citrus volkameriana Ten. & Pasq.); trifoliate orange (Poncirus trifoliata (L.) Raf.); 'Sunki' mandarin (Citrus sunki Hort. ex Tan.) and 'Cleopatra' mandarin (Citrus reshni Hort. ex Tan.). Eleven years after the establishment of the orchard, trees with the greatest canopy development were budded on 'C-13' citrange and 'African' rough lemon, and both differed significantly from trees budded on trifoliate orange, 'Sunki' and 'Cleopatra' mandarins, which presented the smallest canopy development. Trees budded on 'Rangpur' lime and 'C-13' citrange had the highest cumulative yields, and were different from trees budded on trifoliate orange, 'Cleopatra' and 'Sunki' mandarins. There was no rootstock effect on mean fruit weight and on the total soluble solid/acid ratio in the juice. The 'Rangpur' lime and the 'Cleopatra' mandarin rootstocks reduced longevity of plants.


Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2533
Author(s):  
Eduardo Augusto Girardi ◽  
Antonio Juliano Ayres ◽  
Luiz Fernando Girotto ◽  
Leandro Peña

Brazil is the largest producer of sweet orange and its juice in the world. Extensive cultivated area is located under an Aw climate in the North–Northwest of the state of São Paulo and the Triângulo of Minas Gerais state, being subjected to severe drought events. Although 56% of the orchards are irrigated in these regions, there is a need for drought tolerant rootstocks as an alternative to traditional genotypes such as Rangpur lime and Volkamer lemon, which are susceptible to the endemic citrus sudden death disease (CSD). In this sense, the tree size and production of Valencia sweet orange grafted onto 23 rootstock genotypes were evaluated over a ten-year period in rainfed cultivation at 7.0 m × 3.0 m spacing. Most evaluated types resulted from the cross of Poncirus trifoliata with Citrus, but two interspecific hybrids of Citrus (Sunki mandarin × Rangpur lime hybrids), the Barnes trifoliate orange and a tetraploid selection of Swingle citrumelo were also tested. Tropical Sunki mandarin was used as the reference control. Those hybrids coming from the cross of Sunki × Flying Dragon induced large tree sizes to Valencia sweet orange as well as the other citrandarins, Tropical Sunki mandarin and the Sunki mandarin × Rangpur lime hybrids, whereas only the tetraploid Swingle citrumelo behaved as a dwarfing rootstock, decreasing the canopy volume by 77% compared to that induced by the most vigorous citrandarin 535. The citrandarins 543 and 602 and the citrange C38 induced the highest mean fruit production, 67.2 kg·tree−1, but they also caused pronounced alternate bearing and only the hybrid 543 led to a high production efficiency consistently. Graft incompatibility symptoms were not observed over the evaluation period, and the canopy shape of Valencia sweet orange was also influenced by the rootstocks tested. Two citrandarins and one citrange were selected as the most promising alternative rootstocks for Valencia sweet orange grown under an Aw climate, even though productivity would likely benefit from supplementary irrigation.


2017 ◽  
Vol 109 (1) ◽  
pp. 89 ◽  
Author(s):  
Soheil Karimi ◽  
Saeid ESHGHI ◽  
Saeid KARIMI ◽  
Saman HASAN-NEZHADIAN

<p>This study evaluates seed germination and growth of sweet corn under NaCl stress (0, 50, and 100 mM), after exposing the seeds to weak (15 mT) or strong (150 mT) magnetic fields (MF) for different durations (0, 6, 12, and 24 hours). Salinity reduced seed germination and plant growth. MF treatments enhanced rate and percentage of germination and improved plant growth, regardless of salinity. Higher germination rate was obtained by the stronger MF, however, the seedling were more vigorous after priming with 15 mT MF. Proline accumulation was observed in parallel with the loss of plant water content under 100 mM NaCl stress. MF prevented proline accumulation by improving water absorption. Positive correlation between H<sub>2</sub>O<sub>2</sub> accumulation and membrane thermostability (MTI) was found after MF treatments, which revealed that MF primed the plant for salinity by H<sub>2</sub>O<sub>2</sub> signaling. However, over-accumulation of H<sub>2</sub>O<sub>2</sub> after prolonged MF exposure adversely affected MTI under severe salt stress. In conclusion, magnetic priming for 6 hours was suggested for enhancing germination and growth of sweet corn under salt stress.</p>


HortScience ◽  
2009 ◽  
Vol 44 (3) ◽  
pp. 595-598 ◽  
Author(s):  
Giuseppe Reforgiato Recupero ◽  
Giuseppe Russo ◽  
Santo Recupero ◽  
Roberto Zurru ◽  
Bruno Deidda ◽  
...  

In 1968, the CRA-Research Center for Citriculture and Mediterranean Crops (CRA-ACM) started a research program aimed at breeding citrus rootstocks. The monoembryonic species C. latipes (Swing.) Tan. was used as the female parent; trifoliate orange [Poncirus trifoliata (L.) Raf.], sour orange, and volkamer lemon (C. volkameriana Pasq.) were used as male parents. The behavior of some of these hybrids tested with other standard rootstocks in Sicily and Sardinia was evaluated. The cultivars under comparison included ‘Washington’ navel orange and ‘SRA 92’ clementine in Sardinia and ‘Tarocco’ orange in Sicily. Our results showed the dramatic influence of rootstock on plant growth and yield; only minor effects on fruit quality were observed. Among the standard rootstocks tested, Swingle citrumelo provided the highest yield. Some of the tested hybrids (F5 P12, F6 P12, and F6 P13) may improve plant yield, thus maintaining good fruit quality. Encouraging data obtained with these hybrids may justify the use of monoembryonic species of the Papeda subgenus for breeding citrus rootstocks.


2004 ◽  
Vol 61 (1) ◽  
pp. 94-99 ◽  
Author(s):  
Carmen Silvia Vieira Janeiro Neves ◽  
Ives Massanori Murata ◽  
Neusa Maria Colauto Stenzel ◽  
Cristiane de Conti Medina ◽  
Andrey Vetorelli Borges ◽  
...  

Field studies on citrus roots are important for genetic selection of cultivars and for management practices such as localized irrigation and fertilization. To characterize root systems of six rootstocks, taking into consideration chemical and physical characteristics of a clayey Typic Hapludox of the Northern State of Paraná, this study was performed having as scion the 'IAC-5 Tahiti' lime [Citrus latifolia (Yu. Tanaka)]. The rootstocks 'Rangpur' lime (C. limonia Osbeck), 'Africa Rough' lemon (C. jambhiri Lush.), 'Sunki' mandarin [C. sunki (Hayata) hort. ex Tan.], Poncirus trifoliata (L.) Raf., 'C13' citrange [C. sinensis (L.) Osb. x P. trifoliata (L.) Raf] and 'Catânia 2' Volkamer lemon (C. volkameriana Ten. & Pasq.) were used applying the trench profile method and the SIARCS® 3.0 software to determine root distribution. 'C-13' citrange had the largest root system. 'Volkamer' lemon and 'Africa Rough' lemon presented the smallest amount of roots. The effective depth for 80 % of roots was 31-53 cm in rows and 67-68 cm in inter-rows. The effective distance of 80 % of roots measured from the tree trunk exceeded the tree canopy for P. trifoliata, 'Sunki' mandarin, and 'Volkamer' and 'Africa Rough' lemons.


2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Daniela Barrera ◽  
Juan Luera ◽  
Kaitlynn Lavallee ◽  
Pushpa Soti

Abstract Background Using native wildflowers for restoring marginal lands has gained considerable popularity. Establishment of wildflowers can be challenging due to several environmental factors. Restoring the microbial community in degraded habitats can potentially result in the native plant performance and habitat restoration. This study was conducted to investigate the impact of native soil microbes and seeding depth on germination of south Texas native wildflowers. Two wildflower species, Ratibida columnifera (Nutt.) (Mexican Hat) and Verbesina encelioides (Cav.) (cowpen daisy), were treated with microbial wash extracted from native soils, and germination rate was recorded for 14-day period. We further analyzed the growth, biomass allocation, and root colonization by mycorrhizal fungi in these two plants growing them in a plant growth chamber for 6 weeks. To determine the impact of seeding depth, we planted the seeds of the two plant species at 2-cm, 6-cm, and 12-cm depth and monitored germination and plant growth. Results The two species responded differently to the seeding depth and microbial wash treatments. Microbial wash treatment resulted in higher germination rate in R. columnifera compared to control, while it did not have any impact on V. encelioides seed germination. While microbial treatment did not influence the total biomass, it had a significant impact on the biomass allocation in both the plant species. R. columnifera seeds germinated at both 2-cm and 6-cm depth and did not germinate at 12 cm, while the V. encelioides seeds germinated only at 2 cm and did not germinate at 6-cm or 12-cm seeding depth. Conclusions While our results are species specific, our results indicate that native soil microbes can potentially improve the seed germination and growth of wildflowers. Our results also indicate the importance of specific seeding depth when sowing wildflower seeds for habitat restoration.


Author(s):  
Vichai PURIPUNYAVANICH ◽  
Arlee TAMMAN ◽  
Piyanuch ORPONG ◽  
Roppon PICHA ◽  
Mayuree LIMTIYAYOTHIN ◽  
...  

Thailand Plasma Focus 2 (TPF-2) is operated at 2.16 kJ of storage energy with argon as a filled gas and can emit sharp x-ray pulses to plant cells in nanosecond (10-9 s) duration. The effects of plasma focus x-ray on seed germination and seedling growth of 14 Thai rice varieties treated under 10 conditions; 0 (control), 2, 4, 6, 8, 10, 12, 14, 16, and 18 plasma focus shots, were investigated. The results show that the germination rates and growth reduction rates were significantly different among the rice varieties. The germination rate of 10 varieties (Jek Chuey, KDML105, LPT123, PTT1, RD41, RD47, RD49, RD67, RD79, and SPR2) decreased when treated with a high number of plasma focus shots. LD50 and GR50 values were calculated, except for KTH17, RD1, RD7, and RD31 whose germination only slightly decreased, or remained constant, even after they were treated by 18 plasma focus shots. HIGHLIGHTS Effects of plasma focus irradiation on germination and growth were assessed on 14 Thai rice varieties  Shoot and root lengths of rice seedlings were measured 10 days after of plasma treatment Plasma focus was operated at 12 kV and the number of plasma focus shots was varied from 0 (control) to 18 shots Sensitivity to plasma irradiation was found to differ among rice seeds tested GRAPHICAL ABSTRACT


1970 ◽  
Vol 5 (1) ◽  
pp. 104-112
Author(s):  
Arya Agung Pranata ◽  
Asil Barus ◽  
Meiriani

The problem of generative propagation of soursop is its seeds take a long time to germinate which are caused by the hard seed coat. The solution to solve that problem is scarification and coconut water soaking to simply the process of imbibition and seed germination and also stimulate the growth of shoot. This objective of the research was to determine the effect of the scarification position and the soaking with various concentrations of coconut water on seed germination and growth of soursop seedling. This research was held at the Faculty of Agriculture field, the University of Sumatera Utara in March - July 2017. The research used a factorial randomized block design with two factors and three replication. The first factor is the scarification position (without scarification, stomach scarification, top scarification) and the second factor is coconut water soaking (0%; 25%; 50%; 75 %). The result of the research showed that the germination rate parameter, germination percentage, crop height, number of leaves, root volume and dry weight of crop significantly higher on without scarification treatment than with scarification treatment. The interaction between the scarification position and soaking with various coconut water concentrations was not significantly affecting the seed germination and growth of soursop seedling.


2019 ◽  
Vol 10 (4) ◽  
pp. 461-466
Author(s):  
Patrícia Marluci Da Conceição ◽  
Fernando Alves De Azevedo ◽  
Gerhard Vitor Ecker ◽  
Marilia Morelli ◽  
Mariangela Cristofani-Yaly

Rangpur lime is the rootstock most used in Brazil, mainly due to its resistance to drought; however, it is susceptible to several diseases, requiring the search for other rootstocks tolerant to factors limiting citrus cultivation. In addition, the Brazilian legislation stipulates that citrus seeds can only be commercialized with at least 50% germination rate. Thus, the aim of this work was to evaluate the physiological quality of seeds of seven citrus rootstocks. The following citrus fruits were harvested: Poncirus trifoliata, Sunki mandarin and their hybrids –citrandarins (70, 106, 254, 299 and 310). Fruit mass, diameter and height were measured. Seeds were extracted from fruits, counting the number of normal seeds (not aborted). Seeds were dried up to 20% water content and stored for 3, 6, 9 and 12 months. The physiological quality of seeds was evaluated before storage (time 0) up to 12 months of storage. The quality of Poncirus trifoliata, Sunki mandarin and citrandarins 70, 106, 254, 299 and 310 seeds was reduced during storage. However, their germination rate remained above 50%, evidencing that seeds of all rootstocks could be marketed until 12 months of storage. The new rootstocks tested have seeds with physiological quality compatible with their parents, in compliance with current legislation.


2011 ◽  
Vol 46 (4) ◽  
pp. 406-411 ◽  
Author(s):  
Mateus Pereira Gonzatto ◽  
Alisson Pacheco Kovaleski ◽  
Eduardo Cesar Brugnara ◽  
Roberto Luis Weiler ◽  
Ivar Antônio Sartori ◽  
...  

The objective of this work was to assess the effect of six rootstocks on yield, fruit quality, and growth of 'Oneco' mandarin during the first seven harvesting seasons, in Butiá, Rio Grande do Sul State, Brazil. The rootstocks evaluated were: 'Swingle' citrumelo (Citrus paradisi × Poncirus trifoliata), 'Caipira' orange (C. sinensis), 'Troyer' citrange (C. sinensis × P. trifoliata), 'Rangpur' lime (C. limonia), 'Volkamer' lemon (C. volkameriana), and 'Flying Dragon' trifoliata orange (P. trifoliata var. monstrosa). Plants budded onto 'Flying Dragon' had the lowest vegetative development, which indicates the dwarfing characteristics of this rootstock, and had the highest mean production efficiency, despite low yield. Plants grafted on 'Volkamer' lemon and 'Rangpur' lime had the highest alternate bearing. Under the experimental conditions evaluated, the most adequate rootstocks for mandarin 'Oneco' are 'Swingle' citrumelo and 'Troyer' citrange, regarding fruit yield and quality.


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