scholarly journals Enterohemorrhagic Escherichia coli O157: H7 from healthy dairy cattle in Mid-West Brazil: occurrence and molecular characterization

2014 ◽  
Vol 34 (1) ◽  
pp. 24-28 ◽  
Author(s):  
Edismauro G. Freitas Filho ◽  
Marcos R. A. Ferreira ◽  
Jefferson F. N. Pinto ◽  
Fabrício R. Conceição ◽  
Cecília N. Moreira
Keyword(s):  
Escherichia Coli ◽  
Dairy Cattle ◽  
Molecular Characterization ◽  
Enterohemorrhagic Escherichia Coli ◽  
Highly Pathogenic ◽  
E Coli ◽  
Uremic Syndrome ◽  
Group D ◽  
Virulent Group

Enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 represents the major Shiga toxin-producing E. coli (STEC) strain related to large outbreaks and severe diseases such as hemorrhagic colitis (HC) and the potentially lethal hemolytic uremic syndrome (HUS). The aim of this study was to report the occurrence and molecular characterization of O157:H7 isolates obtained by rectal swab from 52 healthy dairy cattle belonging to 21 farms in Mid-West of Brazil. Detection of 16SrRNA, stx1, stx2, rfbO157, fliCh7, eae, ehxA, saa, cnf1, chuA, yjaA and TSPE4.C2 genes was performed by PCR. The isolates were further characterized by serotyping. Two hundred and sixty E. coli isolates were obtained, of which 126 were characterized as STEC. Two isolates from the same cow were identified as serotype O157:H7. Both isolates presented the stx2, eae, ehxA, saa and cnf1 virulence factor genes and the chuA gene in the phylogenetic classification (virulent group D), suggesting that they were clones. The prevalence of O157:H7 was found to be 1.92% (1/52 animals), demonstrating that healthy dairy cattle from farms in the Mid-West of Brazil are an important reservoir for highly pathogenic E. coli O157:H7.

scholarly journals Isolation and Molecular Characterization of Shigatoxigenic O157 and Non-O157 Escherichia coli in Raw Milk Marketed in Chittagong, Bangladesh

2021 ◽  
Author(s):  
Md. Kauser-Ul Alam ◽  
Nazmul Sarwar ◽  
Shireen Akther ◽  
Monsur Ahmad ◽  
Paritosh Kumar Biswas
Keyword(s):  
Escherichia Coli ◽  
Molecular Characterization ◽  
Raw Milk ◽  
Selective Medium ◽  
Enterohemorrhagic Escherichia Coli ◽  
Macconkey Agar ◽  
Biochemical Tests ◽  
E Coli ◽  
Milk Samples

Background: Quality and microbial safety of milk is demanding day by day as it is considered as a host for pathogenic and spoilage microorganisms. In this study, isolation and molecular characterization of shigatoxigenic O157 and non-O157 Escherichia coli in raw milk marketed in Chittagong, Bangladesh were done on 186 raw milk samples in Bangladesh. Methods: MacConkey agar was initially used to screen for the presence of E. coli and the suspected growth as evidenced by large pink colonies on MacConkey agar. Finally the organism was verified by plating through Eosin Methylene Blue (EMB) agar (a selective medium for E. coli where it produces metallic sheen) and applying standard biochemical tests for E. coli. The presence of virulent genes, Shiga-like toxin (stx1 and stx2), Intimin (eaeA), O157 antigen rfbE and Enterohemorrhagic Escherichia coli (EHEC) Hemolysin (EHEC) hlyA in the contaminating E. coli population was determined by polymerase chain reaction (PCR) run on a thermocycler (Applied Biosystem, 2720 thermal cycler, Singapore). Result: Among the raw milk samples, 33 samples were identified as E.coli positive and among the isolates, 6 (18.18%) were identified as possible EHEC O157 and rest of the isolates (81.82%) were considered as probable non EHEC O157. About, 3.23% (186 samples) EHEC O157 was isolated from raw milk samples. Then all the 33 isolates were taken under PCR assay for the identification of five virulent genes Stx1, Stx2, eaeA, rfbE and hlyA. No virulent genes were found in non- EHEC O157 isolates, but 4 stx2 (66.67%) and 1 hlyA (16.67%) gene were observed in another 4 EHEC O157 isolates out of 6, but one isolates contained the both genes and hence the prevalence of STEC was 2.15% in raw milk. Result indicated poor hygienic standard of raw milk from uncontrolled environments and the increased public health risk of those consuming raw milk from such uncontrolled sources.

scholarly journals Characterization of commensal Escherichia coli isolates from slaughtered sheep in Mexico

2021 ◽  
Vol 15 (11) ◽  
pp. 1755-1760
Author(s):  
Jorge Acosta-Dibarrat ◽  
Edgar Enriquez-Gómez ◽  
Martín Talavera-Rojas ◽  
Edgardo Soriano-Vargas ◽  
Armando Navarro ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Multidrug Resistant ◽  
Phylogenetic Group ◽  
Phylogenetic Groups ◽  
E Coli ◽  
New Information ◽  
Antimicrobial Resistance Genes ◽  
Uremic Syndrome

Introduction: Commensal Escherichia coli is defined as bacteria without known virulence factors that could be playing a specific role in some diseases; however, they could be responsible to disseminate antimicrobial resistance genes to other microorganisms. This study aimed to characterize the commensal E. coli isolates obtained from slaughtered sheep in the central region of Mexico. Methodology: Isolates were classified as commensal E. coli when distinctive genes related to diarrheagenic pathotypes (stx1, stx2, eae, bfp, LT, stp, ipaH, and aggR) were discarded by PCR. Identification of serotype, phylogenetic group, and antimicrobial resistance was also performed. Results: A total of 41 isolates were characterized. The phylogenetic groups found were B1 in 37 isolates (90.2%), A in 2 (4.8%), and 1 isolate (2.4%) for C and D groups. Serotypes associated with diarrhea in humans (O104:H2 and O154:NM) and hemolytic uremic syndrome (O8:NM) were detected. Thirty-three isolates (80%) were resistant to ceftazidime, 23 (56%), to tetracycline 8 (19.5%) to ampicillin, and 1 to amikacin. Six isolates (14.6%) were multidrug-resistant. Conclusions: This study provides new information about commensal E. coli in slaughtered sheep, high percentages of resistance to antibiotics, and different profiles of antimicrobial resistance were found, their dissemination constitute a risk factor towards the consuming population.

scholarly journals Escherichia coli O157:H7: distribution, molecular characterization, antimicrobial resistance patterns and source of contamination of sheep and goat carcasses at an export abattoir, Mojdo, Ethiopia

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Solomon Abreham ◽  
Akafete Teklu ◽  
Eric Cox ◽  
Tesfaye Sisay Tessema
Keyword(s):  
Escherichia Coli ◽  
Molecular Characterization ◽  
Virulence Genes ◽  
Latex Agglutination ◽  
Human Infection ◽  
Systematic Random Sampling ◽  
E Coli ◽  
Source Of Infection ◽  
Resistance Patterns

Abstract Background Cattle have been identified as a major reservoir of E. coli O157:H7 for human infection; the ecology of the organism in sheep and goats is less understood. This study was carried out to determine prevalence, source of infection, antibiotic resistance and molecular characterization of Escherichia coli O157: H7 isolated from sheep and goat. Methods Systematic random sampling was carried out at Modjo export abattoir, Ethiopia, from November 2012 to April 2013 to collect 408 samples from 72 sheep and 32 goats. Samples collected were skin swabs, fecal samples, intestinal mucosal swabs and the inside and outside part of carcasses as well as carcass in contacts such as workers hands, knife, hook and carcass washing water. Then, samples were processed following standard bacteriological procedures. Non-Sorbitol fermenting colonies were tested on latex agglutination test and the positives are subjected to PCR for detection of attaching and effacing genes (eaeA) and shiga toxin producing genes (stx1 and stx2). All E. coli O157:H7 isolates were checked for their susceptibility pattern towards 15 selected antibiotics. Results E. coli O157:H7 were detected in only 20/408 samples (4.9%). Among these 20 positive samples, 70% (14/20), 25% (5/20) and 5% (1/20) were from sheep, goats and knife samples, respectively. No significant associations were found between carcasses and the assumed sources of contaminations. Of all the 20 isolates virulence genes were found in 10 (50%) of them; 3 (15%) with only the eaeA gene and 7(35%) expressing eaeA and stx2 genes. All the isolates were susceptible to Norfloxacin (NOR) (100%). Conclusions The presence of virulence genes shows E. coli O157:H7 is a potential source of human infection in Ethiopia.

Characterization of the Risk to Human Health of Pathogenic Escherichia coli Isolates from Chicken Carcasses

1999 ◽  
Vol 62 (7) ◽  
pp. 741-746 ◽  
Author(s):  
FRANÇOIS CAYA ◽  
JOHN M. FAIRBROTHER ◽  
LOUISE LESSARD ◽  
SYLVAIN QUESSY
Keyword(s):  
Escherichia Coli ◽  
Human Health ◽  
Gel Electrophoresis ◽  
Geographical Area ◽  
Pulsed Field Gel Electrophoresis ◽  
E Coli ◽  
Pathogenic Escherichia Coli ◽  
Uremic Syndrome ◽  
Tract Infections

The purpose of this study was to evaluate the risk for human health associated with pathogenic Escherichia coli isolated from airsacculitis and cellulitis in chickens, by comparing the genotypic and phenotypic profiles of avian E. coli isolates and E. coli strains isolated from sick humans during the same period and in the same geographical area as the avian isolates. A total of 96 isolates and 46 isolates from lesions of cellulitis and airsacculitis, respectively, were obtained. Isolates from the backs of some of the affected and healthy birds and 91 intestinal and extraintestinal isolates from humans with diarrhea, urinary tract infections, or septicemia were examined. The frequency of antimicrobial resistance was in general higher in the avian than in the human isolates. VT1-VT2-Eae and VT2-Eae, pathotypes associated with hemolytic and uremic syndrome and bloody diarrhea in humans, were the most frequently encountered pathotypes in human intestinal isolates but were not recovered from the avian isolates. Aero-Pap-TSH and Aero-TSH were the most frequently encountered pathotypes in avian isolates but were rarely observed in human isolates. No avian isolate was of serogroup O157, whereas many human isolates belonged to this O group. O78 and O2 were the most frequently observed O groups in avian isolates but were rarely found in human isolates. Only two avian isolates demonstrated possible relatedness to human isolates based on pulsed-field gel electrophoresis profiles, but they belonged to different pathotypes. Our results suggest that avian isolates recovered from cellulitis and air sacullitis possess very few of the attributes required to cause diseases in humans. It is also concluded that isolates from cellulitis and airsacculitis do not represent a greater hazard than isolates from the back of healthy birds.

Molecular Characterization of Escherichia Coli Isolates from Food Animals

2019 ◽  
Author(s):  
Sabita Debbarma ◽  
Durlav P Bora ◽  
Razibuddin A Hazarika ◽  
Shantanu Tamuly ◽  
Acheenta G Barua ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Characterization ◽  
Molecular Typing ◽  
Uida Gene ◽  
E Coli ◽  
Food Animals ◽  
Meat Samples ◽  
Pcr Targeting ◽  
Repetitive Extragenic Palindromic

The present study was carried out for isolation and molecular characterization of Escherichia coli from faecal and meat samples of food animals viz. cattle, pigs and poultry. A total of 66 E. coli isolates were recovered from 420 samples of different food animals and further confirmed by PCR targeting E. coli specific uidA gene. These isolates were sensitive to chloramphenicol and ciprofloxacin and resistant to ampicillin and cloxacillin. Out of 66 isolates, 42 were typed into 13 different ‘O’ serogroups, 13 untypable and remaining 11 were identified as rough. Serogroup O84, O101, O118, O120 and O147 were predominant and serogroup O118 was found to be common in the samples of all 3 species of food animals. Five (7.57%) and 3 (4.54%) of E. coli isolates were found to harbor virulent genes, stx2 and est, respectively. Twenty representative E. coli isolates selected randomly from 20 different locations were subjected to molecular typing by PCR targeting Repetitive Extragenic Palindromic (REP) sequences. The region specific molecular types of E. coli could not be detected by using REP-PCR based discrimination.

Characterization of Enterohemorrhagic Escherichia coli on Veal Hides and Carcasses

2016 ◽  
Vol 80 (1) ◽  
pp. 136-145 ◽  
Author(s):  
JOSEPH M. BOSILEVAC ◽  
RONG WANG ◽  
BRANDON E. LUEDTKE ◽  
SUSANNE HINKLEY ◽  
TOMMY L. WHEELER ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enterohemorrhagic Escherichia Coli ◽  
Sample Collection ◽  
Foodborne Illnesses ◽  
Screening Assay ◽  
Department Of Agriculture ◽  
E Coli ◽  
Inspection Service ◽  
Collection Date

ABSTRACT Enterohemorrhagic Escherichia coli (EHEC) are Shiga toxin–producing E. coli associated with the most severe forms of foodborne illnesses. The U.S. Department of Agriculture, Food Safety and Inspection Service has identified a higher percentage of non-O157 EHEC compared with E. coli O157:H7–positive samples collected from veal trimmings than from products produced from other cattle slaughter classes. Therefore samples were collected from hides and preevisceration carcasses at five veal processors to assess E. coli O157:H7 and non-O157 EHEC contamination during bob veal and formula-fed veal dressing procedures. E. coli O157:H7 prevalence was measured by culture isolation and found to be on 20.3% of hides and 6.7% of carcasses. In contrast, a non-O157 EHEC molecular screening assay identified 90.3% of hides and 68.2% of carcasses as positive. Only carcass samples were taken forward to culture confirmation and 38.7% yielded one or more non-O157 EHEC isolates. The recovery of an EHEC varied by plant and sample collection date; values ranged from 2.1 to 87.8% among plants and from 4.2 to 64.2% within the same plant. Three plants were resampled after changes were made to sanitary dressing procedures. Between the two collection times at the three plants, hide-to-carcass transfer of E. coli O157:H7 and non-O157 EHEC was significantly reduced. All adulterant EHEC serogroups (O26, O45, O103, O111, O121, and O145) were isolated from veal carcasses as well as four other potentially pathogenic serogroups (O5, O84, O118, and O177). Bob veal was found to have a greater culture prevalence of E. coli O157:H7 and greater positive molecular screens for non-O157 EHEC than formula-fed veal (P < 0.05), but the percentage of culture-confirmed non-O157 EHEC was not different (P > 0.05) between the two types of calves. EHEC-O26, -O111, and -O121 were found more often in bob veal (P < 0.05), whereas EHEC-O103 was found more often in formula-fed veal (P < 0.05).

scholarly journals Molecular Characterization of UpaB and UpaC, Two New Autotransporter Proteins of Uropathogenic Escherichia coli CFT073

2011 ◽  
Vol 80 (1) ◽  
pp. 321-332 ◽  
Author(s):  
Luke P. Allsopp ◽  
Christophe Beloin ◽  
Glen C. Ulett ◽  
Jaione Valle ◽  
Makrina Totsika ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Characterization ◽  
Host Responses ◽  
Upec Strain ◽  
Content Type ◽  
Pcr Screening ◽  
E Coli ◽  
K 12 ◽  
Encoding Genes

ABSTRACTUropathogenicEscherichia coli(UPEC) is the primary cause of urinary tract infection (UTI) in the developed world. The major factors associated with virulence of UPEC are fimbrial adhesins, which mediate specific attachment to host receptors and trigger innate host responses. Another group of adhesins is represented by the autotransporter (AT) subgroup of proteins. The genome-sequenced prototype UPEC strain CFT073 contains 11 putative AT-encoding genes. In this study, we have performed a detailed molecular characterization of two closely related AT adhesins from CFT073: UpaB (c0426) and UpaC (c0478). PCR screening revealed that theupaBandupaCAT-encoding genes are common inE. coli. TheupaBandupaCgenes were cloned and characterized in a recombinantE. coliK-12 strain background. This revealed that they encode proteins located at the cell surface but possess different functional properties: UpaB mediates adherence to several ECM proteins, while UpaC expression is associated with increased biofilm formation. In CFT073,upaBis expressed whileupaCis transcriptionally repressed by the global regulator H-NS. In competitive colonization experiments employing the mouse UTI model, CFT073 significantly outcompeted itsupaB(but notupaC) isogenic mutant strain in the bladder. This attenuated phenotype was also observed in single-challenge experiments, where deletion of theupaBgene in CFT073 significantly reduced early colonization of the bladder.

scholarly journals Presence and Characterization of a Mosaic Genomic Island Which Distinguishes Sorbitol-Fermenting Enterohemorrhagic Escherichia coli O157:H− from E. coli O157:H7

2005 ◽  
Vol 71 (8) ◽  
pp. 4875-4878 ◽  
Author(s):  
Andreas Janka ◽  
Georg Becker ◽  
Anne-Katharina Sonntag ◽  
Martina Bielaszewska ◽  
Ulrich Dobrindt ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genomic Island ◽  
Enterohemorrhagic Escherichia Coli ◽  
Resistance Locus ◽  
Escherichia Coli O157 ◽  
Related Sequence ◽  
E Coli ◽  
Pcr Targeting

ABSTRACT A mosaic genomic island comprising Shigella resistance locus (SRL) sequences flanked by segments of Escherichia coli O157:H7 strain EDL933 O islands 43, 81, and 82 was identified in sorbitol-fermenting (SF) enterohemorrhagic Escherichia coli (EHEC) O157:H− strain 493/89. This mosaic island is absent from strain EDL933. PCR targeting the SRL-related sequence is a useful tool to distinguish SF EHEC O157:H− from EHEC O157:H7.

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